Amino acid dipepetide frequency for Saprolegnia diclina (strain VS20)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.733AlaAla: 12.733 ± 0.067	1.914AlaCys: 1.914 ± 0.019	4.963AlaAsp: 4.963 ± 0.031	4.409AlaGlu: 4.409 ± 0.032	4.021AlaPhe: 4.021 ± 0.027	4.827AlaGly: 4.827 ± 0.038	2.771AlaHis: 2.771 ± 0.021	5.042AlaIle: 5.042 ± 0.026	5.213AlaLys: 5.213 ± 0.037	11.845AlaLeu: 11.845 ± 0.055	3.072AlaMet: 3.072 ± 0.019	3.371AlaAsn: 3.371 ± 0.026	6.53AlaPro: 6.53 ± 0.044	3.716AlaGln: 3.716 ± 0.029	5.657AlaArg: 5.657 ± 0.036	9.218AlaSer: 9.218 ± 0.043	8.556AlaThr: 8.556 ± 0.042	7.033AlaVal: 7.033 ± 0.039	1.389AlaTrp: 1.389 ± 0.013	2.786AlaTyr: 2.786 ± 0.022	0.0AlaXaa: 0.0 ± 0.0
Cys	1.821CysAla: 1.821 ± 0.019	0.384CysCys: 0.384 ± 0.008	1.081CysAsp: 1.081 ± 0.013	0.719CysGlu: 0.719 ± 0.01	0.724CysPhe: 0.724 ± 0.01	1.237CysGly: 1.237 ± 0.015	0.54CysHis: 0.54 ± 0.008	0.88CysIle: 0.88 ± 0.011	0.695CysLys: 0.695 ± 0.011	1.839CysLeu: 1.839 ± 0.015	0.456CysMet: 0.456 ± 0.007	0.596CysAsn: 0.596 ± 0.01	0.982CysPro: 0.982 ± 0.019	0.553CysGln: 0.553 ± 0.008	0.996CysArg: 0.996 ± 0.012	1.248CysSer: 1.248 ± 0.014	1.182CysThr: 1.182 ± 0.013	1.524CysVal: 1.524 ± 0.016	0.218CysTrp: 0.218 ± 0.006	0.506CysTyr: 0.506 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.961AspAla: 7.961 ± 0.04	0.845AspCys: 0.845 ± 0.012	5.802AspAsp: 5.802 ± 0.05	4.253AspGlu: 4.253 ± 0.031	1.809AspPhe: 1.809 ± 0.017	3.886AspGly: 3.886 ± 0.026	1.35AspHis: 1.35 ± 0.013	2.345AspIle: 2.345 ± 0.019	2.048AspLys: 2.048 ± 0.02	4.979AspLeu: 4.979 ± 0.027	1.488AspMet: 1.488 ± 0.014	1.514AspAsn: 1.514 ± 0.016	2.48AspPro: 2.48 ± 0.019	1.439AspGln: 1.439 ± 0.015	2.631AspArg: 2.631 ± 0.021	2.65AspSer: 2.65 ± 0.019	2.872AspThr: 2.872 ± 0.021	5.538AspVal: 5.538 ± 0.027	0.682AspTrp: 0.682 ± 0.009	1.269AspTyr: 1.269 ± 0.016	0.0AspXaa: 0.0 ± 0.0
Glu	6.091GluAla: 6.091 ± 0.044	0.828GluCys: 0.828 ± 0.01	2.772GluAsp: 2.772 ± 0.025	2.692GluGlu: 2.692 ± 0.03	1.54GluPhe: 1.54 ± 0.017	2.171GluGly: 2.171 ± 0.018	1.488GluHis: 1.488 ± 0.014	2.149GluIle: 2.149 ± 0.016	2.597GluLys: 2.597 ± 0.026	5.443GluLeu: 5.443 ± 0.036	1.488GluMet: 1.488 ± 0.014	1.472GluAsn: 1.472 ± 0.015	2.311GluPro: 2.311 ± 0.02	1.574GluGln: 1.574 ± 0.015	3.197GluArg: 3.197 ± 0.026	3.204GluSer: 3.204 ± 0.021	3.487GluThr: 3.487 ± 0.026	3.181GluVal: 3.181 ± 0.023	0.735GluTrp: 0.735 ± 0.01	1.435GluTyr: 1.435 ± 0.015	0.0GluXaa: 0.0 ± 0.0
Phe	3.724PheAla: 3.724 ± 0.026	0.751PheCys: 0.751 ± 0.01	2.202PheAsp: 2.202 ± 0.017	2.059PheGlu: 2.059 ± 0.017	1.526PhePhe: 1.526 ± 0.018	2.811PheGly: 2.811 ± 0.022	0.986PheHis: 0.986 ± 0.01	1.178PheIle: 1.178 ± 0.014	1.248PheLys: 1.248 ± 0.014	3.769PheLeu: 3.769 ± 0.027	0.751PheMet: 0.751 ± 0.01	1.191PheAsn: 1.191 ± 0.014	1.505PhePro: 1.505 ± 0.014	1.403PheGln: 1.403 ± 0.014	1.887PheArg: 1.887 ± 0.017	2.347PheSer: 2.347 ± 0.016	1.916PheThr: 1.916 ± 0.017	3.043PheVal: 3.043 ± 0.022	0.468PheTrp: 0.468 ± 0.008	1.062PheTyr: 1.062 ± 0.012	0.0PheXaa: 0.0 ± 0.0
Gly	5.55GlyAla: 5.55 ± 0.038	1.14GlyCys: 1.14 ± 0.015	3.363GlyAsp: 3.363 ± 0.028	2.133GlyGlu: 2.133 ± 0.019	2.33GlyPhe: 2.33 ± 0.019	3.905GlyGly: 3.905 ± 0.049	1.953GlyHis: 1.953 ± 0.018	2.435GlyIle: 2.435 ± 0.019	2.266GlyLys: 2.266 ± 0.022	5.636GlyLeu: 5.636 ± 0.033	1.262GlyMet: 1.262 ± 0.014	1.939GlyAsn: 1.939 ± 0.019	2.337GlyPro: 2.337 ± 0.022	1.808GlyGln: 1.808 ± 0.016	3.2GlyArg: 3.2 ± 0.024	4.073GlySer: 4.073 ± 0.039	3.177GlyThr: 3.177 ± 0.028	4.265GlyVal: 4.265 ± 0.028	0.793GlyTrp: 0.793 ± 0.011	1.848GlyTyr: 1.848 ± 0.02	0.0GlyXaa: 0.0 ± 0.0
His	3.394HisAla: 3.394 ± 0.024	0.592HisCys: 0.592 ± 0.01	1.753HisAsp: 1.753 ± 0.018	1.653HisGlu: 1.653 ± 0.016	1.002HisPhe: 1.002 ± 0.012	2.053HisGly: 2.053 ± 0.017	0.949HisHis: 0.949 ± 0.013	1.128HisIle: 1.128 ± 0.01	0.999HisLys: 0.999 ± 0.012	3.074HisLeu: 3.074 ± 0.023	0.702HisMet: 0.702 ± 0.01	0.748HisAsn: 0.748 ± 0.01	1.42HisPro: 1.42 ± 0.014	0.934HisGln: 0.934 ± 0.012	1.869HisArg: 1.869 ± 0.018	1.541HisSer: 1.541 ± 0.015	1.502HisThr: 1.502 ± 0.014	2.665HisVal: 2.665 ± 0.023	0.435HisTrp: 0.435 ± 0.008	0.725HisTyr: 0.725 ± 0.009	0.0HisXaa: 0.0 ± 0.0
Ile	4.735IleAla: 4.735 ± 0.027	0.747IleCys: 0.747 ± 0.009	2.568IleAsp: 2.568 ± 0.02	2.481IleGlu: 2.481 ± 0.017	1.467IlePhe: 1.467 ± 0.016	2.418IleGly: 2.418 ± 0.022	0.942IleHis: 0.942 ± 0.01	1.285IleIle: 1.285 ± 0.015	1.665IleLys: 1.665 ± 0.017	3.74IleLeu: 3.74 ± 0.029	0.824IleMet: 0.824 ± 0.011	1.259IleAsn: 1.259 ± 0.013	1.848IlePro: 1.848 ± 0.014	1.604IleGln: 1.604 ± 0.014	1.905IleArg: 1.905 ± 0.017	2.448IleSer: 2.448 ± 0.018	2.084IleThr: 2.084 ± 0.017	3.438IleVal: 3.438 ± 0.021	0.446IleTrp: 0.446 ± 0.007	1.116IleTyr: 1.116 ± 0.011	0.0IleXaa: 0.0 ± 0.0
Lys	4.572LysAla: 4.572 ± 0.032	0.688LysCys: 0.688 ± 0.011	2.263LysAsp: 2.263 ± 0.02	2.283LysGlu: 2.283 ± 0.02	1.123LysPhe: 1.123 ± 0.013	1.915LysGly: 1.915 ± 0.018	1.265LysHis: 1.265 ± 0.013	1.533LysIle: 1.533 ± 0.014	2.928LysLys: 2.928 ± 0.04	4.133LysLeu: 4.133 ± 0.029	1.05LysMet: 1.05 ± 0.012	1.207LysAsn: 1.207 ± 0.014	2.303LysPro: 2.303 ± 0.022	1.742LysGln: 1.742 ± 0.02	3.075LysArg: 3.075 ± 0.027	2.64LysSer: 2.64 ± 0.019	2.792LysThr: 2.792 ± 0.022	2.605LysVal: 2.605 ± 0.021	0.555LysTrp: 0.555 ± 0.009	1.194LysTyr: 1.194 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	10.917LeuAla: 10.917 ± 0.051	2.035LeuCys: 2.035 ± 0.019	6.155LeuAsp: 6.155 ± 0.036	5.979LeuGlu: 5.979 ± 0.041	3.706LeuPhe: 3.706 ± 0.028	5.886LeuGly: 5.886 ± 0.033	3.796LeuHis: 3.796 ± 0.029	2.865LeuIle: 2.865 ± 0.02	3.873LeuLys: 3.873 ± 0.028	12.293LeuLeu: 12.293 ± 0.07	2.001LeuMet: 2.001 ± 0.016	2.811LeuAsn: 2.811 ± 0.021	5.903LeuPro: 5.903 ± 0.03	5.392LeuGln: 5.392 ± 0.033	7.141LeuArg: 7.141 ± 0.038	7.548LeuSer: 7.548 ± 0.032	5.7LeuThr: 5.7 ± 0.027	7.97LeuVal: 7.97 ± 0.044	1.349LeuTrp: 1.349 ± 0.016	2.806LeuTyr: 2.806 ± 0.02	0.0LeuXaa: 0.0 ± 0.0
Met	2.94MetAla: 2.94 ± 0.018	0.363MetCys: 0.363 ± 0.007	1.629MetAsp: 1.629 ± 0.016	1.338MetGlu: 1.338 ± 0.014	0.662MetPhe: 0.662 ± 0.01	1.313MetGly: 1.313 ± 0.013	0.792MetHis: 0.792 ± 0.011	0.726MetIle: 0.726 ± 0.011	0.923MetLys: 0.923 ± 0.012	2.597MetLeu: 2.597 ± 0.02	0.616MetMet: 0.616 ± 0.01	0.716MetAsn: 0.716 ± 0.009	1.22MetPro: 1.22 ± 0.013	1.141MetGln: 1.141 ± 0.011	1.396MetArg: 1.396 ± 0.014	1.607MetSer: 1.607 ± 0.015	1.755MetThr: 1.755 ± 0.016	1.552MetVal: 1.552 ± 0.013	0.295MetTrp: 0.295 ± 0.007	0.736MetTyr: 0.736 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.491AsnAla: 3.491 ± 0.028	0.547AsnCys: 0.547 ± 0.009	2.076AsnAsp: 2.076 ± 0.017	1.687AsnGlu: 1.687 ± 0.016	0.946AsnPhe: 0.946 ± 0.011	2.277AsnGly: 2.277 ± 0.023	0.741AsnHis: 0.741 ± 0.009	1.247AsnIle: 1.247 ± 0.014	1.165AsnLys: 1.165 ± 0.015	2.649AsnLeu: 2.649 ± 0.019	0.783AsnMet: 0.783 ± 0.01	1.093AsnAsn: 1.093 ± 0.015	1.504AsnPro: 1.504 ± 0.015	1.086AsnGln: 1.086 ± 0.011	1.373AsnArg: 1.373 ± 0.012	1.644AsnSer: 1.644 ± 0.013	1.658AsnThr: 1.658 ± 0.016	2.71AsnVal: 2.71 ± 0.019	0.408AsnTrp: 0.408 ± 0.008	0.807AsnTyr: 0.807 ± 0.009	0.0AsnXaa: 0.0 ± 0.0
Pro	5.302ProAla: 5.302 ± 0.031	0.717ProCys: 0.717 ± 0.012	2.444ProAsp: 2.444 ± 0.02	2.583ProGlu: 2.583 ± 0.02	1.757ProPhe: 1.757 ± 0.016	2.366ProGly: 2.366 ± 0.022	1.34ProHis: 1.34 ± 0.014	2.045ProIle: 2.045 ± 0.015	2.288ProLys: 2.288 ± 0.02	5.294ProLeu: 5.294 ± 0.033	1.393ProMet: 1.393 ± 0.012	1.605ProAsn: 1.605 ± 0.015	4.465ProPro: 4.465 ± 0.042	1.578ProGln: 1.578 ± 0.017	3.236ProArg: 3.236 ± 0.025	5.477ProSer: 5.477 ± 0.035	4.925ProThr: 4.925 ± 0.038	3.637ProVal: 3.637 ± 0.029	0.684ProTrp: 0.684 ± 0.009	1.231ProTyr: 1.231 ± 0.013	0.0ProXaa: 0.0 ± 0.0
Gln	3.936GlnAla: 3.936 ± 0.03	0.783GlnCys: 0.783 ± 0.011	1.671GlnAsp: 1.671 ± 0.017	1.678GlnGlu: 1.678 ± 0.018	1.223GlnPhe: 1.223 ± 0.013	1.718GlnGly: 1.718 ± 0.013	1.312GlnHis: 1.312 ± 0.015	1.347GlnIle: 1.347 ± 0.012	1.531GlnLys: 1.531 ± 0.016	4.628GlnLeu: 4.628 ± 0.032	0.883GlnMet: 0.883 ± 0.01	0.953GlnAsn: 0.953 ± 0.01	1.824GlnPro: 1.824 ± 0.017	1.89GlnGln: 1.89 ± 0.029	2.954GlnArg: 2.954 ± 0.022	2.532GlnSer: 2.532 ± 0.021	2.121GlnThr: 2.121 ± 0.019	2.467GlnVal: 2.467 ± 0.018	0.682GlnTrp: 0.682 ± 0.009	1.123GlnTyr: 1.123 ± 0.012	0.0GlnXaa: 0.0 ± 0.0
Arg	5.584ArgAla: 5.584 ± 0.032	1.124ArgCys: 1.124 ± 0.013	3.271ArgAsp: 3.271 ± 0.025	2.713ArgGlu: 2.713 ± 0.023	2.235ArgPhe: 2.235 ± 0.018	3.075ArgGly: 3.075 ± 0.024	2.017ArgHis: 2.017 ± 0.017	2.407ArgIle: 2.407 ± 0.018	2.674ArgLys: 2.674 ± 0.024	6.463ArgLeu: 6.463 ± 0.037	1.345ArgMet: 1.345 ± 0.015	1.736ArgAsn: 1.736 ± 0.014	2.941ArgPro: 2.941 ± 0.023	2.5ArgGln: 2.5 ± 0.019	4.82ArgArg: 4.82 ± 0.045	4.112ArgSer: 4.112 ± 0.026	3.108ArgThr: 3.108 ± 0.02	4.144ArgVal: 4.144 ± 0.026	0.852ArgTrp: 0.852 ± 0.012	1.576ArgTyr: 1.576 ± 0.015	0.0ArgXaa: 0.0 ± 0.0
Ser	7.118SerAla: 7.118 ± 0.037	1.223SerCys: 1.223 ± 0.012	3.821SerAsp: 3.821 ± 0.025	2.866SerGlu: 2.866 ± 0.019	2.912SerPhe: 2.912 ± 0.019	3.972SerGly: 3.972 ± 0.046	1.76SerHis: 1.76 ± 0.014	3.384SerIle: 3.384 ± 0.025	2.668SerLys: 2.668 ± 0.023	7.622SerLeu: 7.622 ± 0.038	2.02SerMet: 2.02 ± 0.017	2.168SerAsn: 2.168 ± 0.017	4.456SerPro: 4.456 ± 0.032	2.142SerGln: 2.142 ± 0.019	3.623SerArg: 3.623 ± 0.024	7.108SerSer: 7.108 ± 0.046	5.384SerThr: 5.384 ± 0.033	5.363SerVal: 5.363 ± 0.035	0.945SerTrp: 0.945 ± 0.01	1.922SerTyr: 1.922 ± 0.018	0.0SerXaa: 0.0 ± 0.0
Thr	6.257ThrAla: 6.257 ± 0.037	1.179ThrCys: 1.179 ± 0.015	3.029ThrAsp: 3.029 ± 0.02	2.731ThrGlu: 2.731 ± 0.02	2.381ThrPhe: 2.381 ± 0.019	3.071ThrGly: 3.071 ± 0.032	1.617ThrHis: 1.617 ± 0.014	2.845ThrIle: 2.845 ± 0.02	3.005ThrLys: 3.005 ± 0.023	6.972ThrLeu: 6.972 ± 0.035	1.624ThrMet: 1.624 ± 0.015	2.075ThrAsn: 2.075 ± 0.018	4.559ThrPro: 4.559 ± 0.033	2.196ThrGln: 2.196 ± 0.019	3.192ThrArg: 3.192 ± 0.02	5.635ThrSer: 5.635 ± 0.033	5.707ThrThr: 5.707 ± 0.043	4.026ThrVal: 4.026 ± 0.031	0.895ThrTrp: 0.895 ± 0.011	1.824ThrTyr: 1.824 ± 0.017	0.0ThrXaa: 0.0 ± 0.0
Val	8.989ValAla: 8.989 ± 0.045	1.424ValCys: 1.424 ± 0.013	4.594ValAsp: 4.594 ± 0.025	3.643ValGlu: 3.643 ± 0.025	2.792ValPhe: 2.792 ± 0.021	4.031ValGly: 4.031 ± 0.028	2.341ValHis: 2.341 ± 0.019	2.475ValIle: 2.475 ± 0.019	2.452ValLys: 2.452 ± 0.021	8.702ValLeu: 8.702 ± 0.047	1.468ValMet: 1.468 ± 0.014	2.001ValAsn: 2.001 ± 0.016	4.007ValPro: 4.007 ± 0.027	3.095ValGln: 3.095 ± 0.024	4.085ValArg: 4.085 ± 0.023	4.792ValSer: 4.792 ± 0.032	4.242ValThr: 4.242 ± 0.033	6.724ValVal: 6.724 ± 0.035	0.954ValTrp: 0.954 ± 0.011	2.192ValTyr: 2.192 ± 0.017	0.0ValXaa: 0.0 ± 0.0
Trp	1.124TrpAla: 1.124 ± 0.015	0.265TrpCys: 0.265 ± 0.006	0.684TrpAsp: 0.684 ± 0.009	0.498TrpGlu: 0.498 ± 0.008	0.522TrpPhe: 0.522 ± 0.007	0.625TrpGly: 0.625 ± 0.01	0.444TrpHis: 0.444 ± 0.009	0.552TrpIle: 0.552 ± 0.009	0.578TrpLys: 0.578 ± 0.009	1.527TrpLeu: 1.527 ± 0.016	0.338TrpMet: 0.338 ± 0.007	0.46TrpAsn: 0.46 ± 0.007	0.631TrpPro: 0.631 ± 0.011	0.57TrpGln: 0.57 ± 0.01	1.0TrpArg: 1.0 ± 0.016	1.06TrpSer: 1.06 ± 0.012	1.023TrpThr: 1.023 ± 0.014	0.864TrpVal: 0.864 ± 0.012	0.187TrpTrp: 0.187 ± 0.005	0.392TrpTyr: 0.392 ± 0.007	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.825TyrAla: 2.825 ± 0.02	0.632TyrCys: 0.632 ± 0.009	1.701TyrAsp: 1.701 ± 0.016	1.458TyrGlu: 1.458 ± 0.019	1.149TyrPhe: 1.149 ± 0.013	1.859TyrGly: 1.859 ± 0.019	0.735TyrHis: 0.735 ± 0.01	1.153TyrIle: 1.153 ± 0.011	1.049TyrLys: 1.049 ± 0.012	2.918TyrLeu: 2.918 ± 0.023	0.751TyrMet: 0.751 ± 0.008	0.975TyrAsn: 0.975 ± 0.011	1.142TyrPro: 1.142 ± 0.013	0.891TyrGln: 0.891 ± 0.01	1.46TyrArg: 1.46 ± 0.014	1.593TyrSer: 1.593 ± 0.015	1.631TyrThr: 1.631 ± 0.016	2.255TyrVal: 2.255 ± 0.019	0.375TyrTrp: 0.375 ± 0.007	0.951TyrTyr: 0.951 ± 0.011	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 18047 proteins (8784450 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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