Croton yellow vein mosaic virus
Average proteome isoelectric point is 7.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8B8N5|Q8B8N5_9GEMI Transcriptional activator protein OS=Croton yellow vein mosaic virus OX=207697 GN=AC2 PE=3 SV=1
MM1 pKa = 7.45 PRR3 pKa = 11.84 INSFCVNAKK12 pKa = 10.44 NIFLTYY18 pKa = 8.78 PKK20 pKa = 10.44 CPIPKK25 pKa = 9.32 EE26 pKa = 3.88 QMLEE30 pKa = 3.64 ILKK33 pKa = 10.73 NISCPSNRR41 pKa = 11.84 LFIRR45 pKa = 11.84 VAQEE49 pKa = 3.49 KK50 pKa = 9.43 HH51 pKa = 5.42 QDD53 pKa = 3.28 GSMHH57 pKa = 5.64 IHH59 pKa = 7.06 ALIQFKK65 pKa = 10.72 GKK67 pKa = 10.47 SKK69 pKa = 10.22 FRR71 pKa = 11.84 NPRR74 pKa = 11.84 HH75 pKa = 6.17 FDD77 pKa = 3.17 VTHH80 pKa = 7.04 PNSSTQFHH88 pKa = 7.29 PNFQGAKK95 pKa = 9.08 SSSDD99 pKa = 3.06 VKK101 pKa = 11.23 SYY103 pKa = 10.83 IEE105 pKa = 4.63 KK106 pKa = 10.79 DD107 pKa = 2.7 SDD109 pKa = 4.05 YY110 pKa = 11.01 IDD112 pKa = 3.23 WGEE115 pKa = 3.76 FQIDD119 pKa = 3.21 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGQQTANDD132 pKa = 3.91 AAAEE136 pKa = 3.94 ALNAGSTEE144 pKa = 3.66 AALAIIRR151 pKa = 11.84 EE152 pKa = 4.23 KK153 pKa = 10.87 LPKK156 pKa = 10.46 DD157 pKa = 4.77 FIFQYY162 pKa = 11.21 HH163 pKa = 5.5 NLKK166 pKa = 10.79 CNLDD170 pKa = 4.15 RR171 pKa = 11.84 IFTPPMEE178 pKa = 4.56 VYY180 pKa = 10.67 VSPFSSSSFDD190 pKa = 3.41 QVPEE194 pKa = 3.98 EE195 pKa = 4.13 LEE197 pKa = 3.58 EE198 pKa = 3.9 WAAEE202 pKa = 4.15 NVVSAAARR210 pKa = 11.84 PIRR213 pKa = 11.84 PISIVIEE220 pKa = 4.23 GDD222 pKa = 3.2 SRR224 pKa = 11.84 TGKK227 pKa = 8.52 TMWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.38 LDD245 pKa = 4.18 LSPKK249 pKa = 10.15 VYY251 pKa = 11.07 SNDD254 pKa = 2.25 AWYY257 pKa = 10.92 NVIDD261 pKa = 5.2 DD262 pKa = 4.41 VDD264 pKa = 3.61 PHH266 pKa = 5.85 YY267 pKa = 11.0 LKK269 pKa = 10.7 HH270 pKa = 6.2 FKK272 pKa = 10.76 EE273 pKa = 4.49 FMGAQKK279 pKa = 10.58 DD280 pKa = 3.71 WQSNTKK286 pKa = 9.54 YY287 pKa = 10.57 GKK289 pKa = 9.12 PVQIKK294 pKa = 10.41 GGIPTIFLCNPGPDD308 pKa = 3.27 SSYY311 pKa = 11.58 KK312 pKa = 10.6 EE313 pKa = 3.96 FLDD316 pKa = 3.79 EE317 pKa = 4.63 EE318 pKa = 4.37 KK319 pKa = 11.05 NAALKK324 pKa = 10.45 NWALKK329 pKa = 10.17 NATFITLEE337 pKa = 4.26 GPLYY341 pKa = 10.84 SGSNQSAAQDD351 pKa = 3.81 SQEE354 pKa = 3.99 GDD356 pKa = 3.33 QASTSS361 pKa = 3.49
Molecular weight: 40.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.952
IPC2_protein 5.906
IPC_protein 5.982
Toseland 6.376
ProMoST 6.338
Dawson 6.287
Bjellqvist 6.275
Wikipedia 6.3
Rodwell 6.275
Grimsley 6.517
Solomon 6.287
Lehninger 6.275
Nozaki 6.561
DTASelect 6.751
Thurlkill 6.766
EMBOSS 6.737
Sillero 6.664
Patrickios 4.202
IPC_peptide 6.3
IPC2_peptide 6.59
IPC2.peptide.svr19 6.607
Protein with the highest isoelectric point:
>tr|Q8B8N8|Q8B8N8_9GEMI AC5 protein OS=Croton yellow vein mosaic virus OX=207697 GN=AC5 PE=4 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.87 IIISTPASKK16 pKa = 10.3 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.07 SPYY27 pKa = 9.85 VNRR30 pKa = 11.84 AAAPIVRR37 pKa = 11.84 VTKK40 pKa = 10.71 AKK42 pKa = 10.29 AWANRR47 pKa = 11.84 PMYY50 pKa = 9.8 RR51 pKa = 11.84 KK52 pKa = 8.75 PRR54 pKa = 11.84 MYY56 pKa = 10.82 RR57 pKa = 11.84 MYY59 pKa = 10.57 RR60 pKa = 11.84 SPDD63 pKa = 3.29 VPKK66 pKa = 10.81 GCEE69 pKa = 4.39 GPCKK73 pKa = 10.37 VQSFDD78 pKa = 3.26 AKK80 pKa = 11.06 NDD82 pKa = 3.27 IGHH85 pKa = 6.11 MGKK88 pKa = 10.22 VICLSDD94 pKa = 3.33 VTRR97 pKa = 11.84 GIGLTHH103 pKa = 6.67 RR104 pKa = 11.84 VGKK107 pKa = 9.78 RR108 pKa = 11.84 FCVKK112 pKa = 10.05 SLYY115 pKa = 10.31 FVGKK119 pKa = 9.14 IWMDD123 pKa = 3.43 EE124 pKa = 4.06 NIKK127 pKa = 9.32 TKK129 pKa = 10.61 NHH131 pKa = 6.02 TNTVMFWIVRR141 pKa = 11.84 DD142 pKa = 3.87 RR143 pKa = 11.84 RR144 pKa = 11.84 PSGTPNDD151 pKa = 3.78 FQQVFNVYY159 pKa = 10.67 DD160 pKa = 4.09 NEE162 pKa = 4.37 PSTATVKK169 pKa = 10.59 NDD171 pKa = 3.0 QRR173 pKa = 11.84 DD174 pKa = 3.36 RR175 pKa = 11.84 FQVLRR180 pKa = 11.84 RR181 pKa = 11.84 FQATVTGGQYY191 pKa = 10.18 AAKK194 pKa = 8.87 EE195 pKa = 3.58 QAIIRR200 pKa = 11.84 KK201 pKa = 8.88 FYY203 pKa = 10.48 RR204 pKa = 11.84 VNNYY208 pKa = 7.79 VVYY211 pKa = 10.32 NHH213 pKa = 6.0 QEE215 pKa = 3.43 AGKK218 pKa = 10.41 YY219 pKa = 8.39 EE220 pKa = 4.09 NHH222 pKa = 6.52 TEE224 pKa = 3.99 NALLLYY230 pKa = 7.29 MACTHH235 pKa = 7.07 ASNPVYY241 pKa = 9.99 ATLKK245 pKa = 8.67 VRR247 pKa = 11.84 SYY249 pKa = 10.98 FYY251 pKa = 11.25 DD252 pKa = 3.13 SVTNN256 pKa = 3.98
Molecular weight: 29.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.265
IPC2_protein 9.545
IPC_protein 9.765
Toseland 10.072
ProMoST 9.823
Dawson 10.306
Bjellqvist 10.014
Wikipedia 10.496
Rodwell 10.643
Grimsley 10.379
Solomon 10.321
Lehninger 10.292
Nozaki 10.101
DTASelect 9.999
Thurlkill 10.145
EMBOSS 10.482
Sillero 10.218
Patrickios 10.058
IPC_peptide 10.321
IPC2_peptide 8.873
IPC2.peptide.svr19 8.527
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1195
85
361
170.7
19.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.941 ± 0.859
2.259 ± 0.479
4.937 ± 0.473
4.268 ± 0.599
4.351 ± 0.498
4.937 ± 0.426
4.017 ± 0.754
5.941 ± 0.534
5.607 ± 0.835
6.276 ± 0.808
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.51 ± 0.652
5.774 ± 0.622
6.36 ± 0.71
4.77 ± 0.501
6.444 ± 0.897
8.368 ± 1.64
5.607 ± 0.593
6.527 ± 0.972
1.674 ± 0.381
3.431 ± 0.689
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here