Streptococcus phage IPP27

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SAR8|A0A1S5SAR8_9CAUD Uncharacterized protein OS=Streptococcus phage IPP27 OX=1916167 GN=IPP27_00010 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.59KK3 pKa = 9.98NDD5 pKa = 3.55LFIDD9 pKa = 4.05VSSHH13 pKa = 5.28NGYY16 pKa = 10.62DD17 pKa = 3.0ITGILANMGTQNTIIKK33 pKa = 9.88ISEE36 pKa = 3.99STNYY40 pKa = 10.47LNPCLSAQIEE50 pKa = 4.1QSNPVGFYY58 pKa = 10.05HH59 pKa = 6.6FAWFGGDD66 pKa = 2.88IEE68 pKa = 4.28EE69 pKa = 4.71AEE71 pKa = 3.98RR72 pKa = 11.84EE73 pKa = 3.93ARR75 pKa = 11.84YY76 pKa = 9.33FLDD79 pKa = 4.07NVPQKK84 pKa = 11.06VKK86 pKa = 10.38YY87 pKa = 10.42LCLDD91 pKa = 3.96YY92 pKa = 10.92EE93 pKa = 4.44DD94 pKa = 5.0HH95 pKa = 7.98ASGDD99 pKa = 3.59KK100 pKa = 10.11QANTDD105 pKa = 2.87ACIRR109 pKa = 11.84FMEE112 pKa = 4.15ILKK115 pKa = 10.52EE116 pKa = 3.95NGYY119 pKa = 10.25EE120 pKa = 4.01PIYY123 pKa = 10.7YY124 pKa = 9.76SYY126 pKa = 11.64KK127 pKa = 10.54PFTLNNIYY135 pKa = 10.49YY136 pKa = 8.85EE137 pKa = 4.13QILAKK142 pKa = 10.21FPNSLWIAGYY152 pKa = 10.38GLNDD156 pKa = 3.34GNADD160 pKa = 3.61FEE162 pKa = 5.01YY163 pKa = 10.02FPSMDD168 pKa = 4.51GIRR171 pKa = 11.84WWQYY175 pKa = 10.51SSNPYY180 pKa = 10.19DD181 pKa = 3.89KK182 pKa = 11.26NIVLLDD188 pKa = 3.93DD189 pKa = 5.01EE190 pKa = 4.48EE191 pKa = 5.67AKK193 pKa = 10.77PKK195 pKa = 9.45WKK197 pKa = 10.61RR198 pKa = 11.84NDD200 pKa = 3.37TGWWYY205 pKa = 9.68EE206 pKa = 4.17YY207 pKa = 10.63PNGAYY212 pKa = 9.41PKK214 pKa = 10.07EE215 pKa = 4.05EE216 pKa = 3.92WEE218 pKa = 4.62KK219 pKa = 10.87IDD221 pKa = 4.81GIWYY225 pKa = 9.08YY226 pKa = 10.9FDD228 pKa = 3.22EE229 pKa = 5.15RR230 pKa = 11.84GYY232 pKa = 10.89SIASRR237 pKa = 11.84WLKK240 pKa = 11.05DD241 pKa = 3.4DD242 pKa = 3.93GKK244 pKa = 10.08WYY246 pKa = 9.81YY247 pKa = 10.97LKK249 pKa = 10.83EE250 pKa = 4.24NGEE253 pKa = 4.2MAIGWVFVNGKK264 pKa = 8.62WYY266 pKa = 10.87YY267 pKa = 10.95LDD269 pKa = 4.4ASGAMVTGWVQYY281 pKa = 10.6KK282 pKa = 10.69DD283 pKa = 3.29KK284 pKa = 10.79LYY286 pKa = 10.72HH287 pKa = 6.22LKK289 pKa = 10.64EE290 pKa = 4.38EE291 pKa = 4.2NGEE294 pKa = 4.09MSSKK298 pKa = 10.61EE299 pKa = 3.88LVKK302 pKa = 10.95VEE304 pKa = 3.94GGWYY308 pKa = 9.42YY309 pKa = 11.32VNEE312 pKa = 4.72DD313 pKa = 3.49GSRR316 pKa = 11.84SDD318 pKa = 4.45KK319 pKa = 10.76PAFDD323 pKa = 4.03VLPDD327 pKa = 3.69GLITTKK333 pKa = 10.88

Molecular weight: 39.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SAG1|A0A1S5SAG1_9CAUD Uncharacterized protein OS=Streptococcus phage IPP27 OX=1916167 GN=IPP27_00048 PE=4 SV=1
MM1 pKa = 6.99VKK3 pKa = 10.05NRR5 pKa = 11.84ASVTSGYY12 pKa = 10.24IIDD15 pKa = 3.56VTARR19 pKa = 11.84KK20 pKa = 9.6VSYY23 pKa = 10.78INGLSAIFDD32 pKa = 4.39KK33 pKa = 10.89ILKK36 pKa = 10.16KK37 pKa = 11.19VMM39 pKa = 3.8

Molecular weight: 4.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

10243

39

873

209.0

23.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.561 ± 0.395

0.527 ± 0.097

6.365 ± 0.314

7.703 ± 0.328

4.1 ± 0.216

6.004 ± 0.428

1.425 ± 0.16

7.185 ± 0.294

8.904 ± 0.39

8.806 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.165

6.151 ± 0.188

2.646 ± 0.178

3.778 ± 0.22

4.149 ± 0.393

5.887 ± 0.255

6.141 ± 0.232

6.004 ± 0.252

1.191 ± 0.175

3.895 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski