Amino acid dipepetide frequency for Streptococcus phage IPP27

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.929AlaAla: 2.929 ± 0.634
0.488AlaCys: 0.488 ± 0.192
4.101AlaAsp: 4.101 ± 0.495
6.346AlaGlu: 6.346 ± 0.664
2.05AlaPhe: 2.05 ± 0.5
4.101AlaGly: 4.101 ± 0.587
0.879AlaHis: 0.879 ± 0.261
5.956AlaIle: 5.956 ± 0.638
5.956AlaLys: 5.956 ± 0.923
4.491AlaLeu: 4.491 ± 0.756
1.562AlaMet: 1.562 ± 0.359
4.198AlaAsn: 4.198 ± 0.628
1.074AlaPro: 1.074 ± 0.26
3.027AlaGln: 3.027 ± 0.578
2.734AlaArg: 2.734 ± 0.483
4.491AlaSer: 4.491 ± 0.697
4.687AlaThr: 4.687 ± 0.721
4.003AlaVal: 4.003 ± 0.78
0.586AlaTrp: 0.586 ± 0.259
1.953AlaTyr: 1.953 ± 0.405
0.0AlaXaa: 0.0 ± 0.0
Cys
0.098CysAla: 0.098 ± 0.092
0.0CysCys: 0.0 ± 0.0
0.586CysAsp: 0.586 ± 0.217
0.488CysGlu: 0.488 ± 0.243
0.098CysPhe: 0.098 ± 0.101
0.195CysGly: 0.195 ± 0.173
0.098CysHis: 0.098 ± 0.099
0.195CysIle: 0.195 ± 0.146
0.195CysLys: 0.195 ± 0.134
0.586CysLeu: 0.586 ± 0.265
0.098CysMet: 0.098 ± 0.096
0.293CysAsn: 0.293 ± 0.173
0.293CysPro: 0.293 ± 0.174
0.098CysGln: 0.098 ± 0.099
0.391CysArg: 0.391 ± 0.232
0.391CysSer: 0.391 ± 0.185
0.098CysThr: 0.098 ± 0.098
0.293CysVal: 0.293 ± 0.164
0.195CysTrp: 0.195 ± 0.144
0.586CysTyr: 0.586 ± 0.257
0.0CysXaa: 0.0 ± 0.0
Asp
4.589AspAla: 4.589 ± 0.816
0.391AspCys: 0.391 ± 0.194
4.784AspAsp: 4.784 ± 0.818
4.979AspGlu: 4.979 ± 0.604
3.417AspPhe: 3.417 ± 0.495
4.882AspGly: 4.882 ± 0.853
0.879AspHis: 0.879 ± 0.275
4.589AspIle: 4.589 ± 0.654
4.979AspLys: 4.979 ± 0.721
5.956AspLeu: 5.956 ± 1.015
1.757AspMet: 1.757 ± 0.35
4.589AspAsn: 4.589 ± 0.798
1.953AspPro: 1.953 ± 0.402
1.074AspGln: 1.074 ± 0.409
1.757AspArg: 1.757 ± 0.437
3.417AspSer: 3.417 ± 0.393
2.636AspThr: 2.636 ± 0.503
3.124AspVal: 3.124 ± 0.541
0.781AspTrp: 0.781 ± 0.297
3.124AspTyr: 3.124 ± 0.471
0.0AspXaa: 0.0 ± 0.0
Glu
5.272GluAla: 5.272 ± 0.692
0.391GluCys: 0.391 ± 0.209
3.905GluAsp: 3.905 ± 0.563
4.589GluGlu: 4.589 ± 0.87
2.246GluPhe: 2.246 ± 0.374
3.71GluGly: 3.71 ± 0.602
1.367GluHis: 1.367 ± 0.46
6.151GluIle: 6.151 ± 0.893
6.054GluLys: 6.054 ± 0.924
7.811GluLeu: 7.811 ± 0.988
1.66GluMet: 1.66 ± 0.462
4.101GluAsn: 4.101 ± 0.826
1.367GluPro: 1.367 ± 0.402
4.101GluGln: 4.101 ± 0.628
3.905GluArg: 3.905 ± 0.716
4.589GluSer: 4.589 ± 0.554
5.077GluThr: 5.077 ± 0.681
5.565GluVal: 5.565 ± 0.925
1.269GluTrp: 1.269 ± 0.338
3.905GluTyr: 3.905 ± 0.508
0.0GluXaa: 0.0 ± 0.0
Phe
2.05PheAla: 2.05 ± 0.421
0.098PheCys: 0.098 ± 0.096
3.32PheAsp: 3.32 ± 0.653
4.198PheGlu: 4.198 ± 0.685
2.636PhePhe: 2.636 ± 0.614
2.929PheGly: 2.929 ± 0.658
0.293PheHis: 0.293 ± 0.172
1.367PheIle: 1.367 ± 0.548
3.222PheLys: 3.222 ± 0.538
3.027PheLeu: 3.027 ± 0.401
1.074PheMet: 1.074 ± 0.323
2.539PheAsn: 2.539 ± 0.525
0.879PhePro: 0.879 ± 0.382
1.66PheGln: 1.66 ± 0.338
1.269PheArg: 1.269 ± 0.371
2.246PheSer: 2.246 ± 0.563
2.734PheThr: 2.734 ± 0.406
3.222PheVal: 3.222 ± 0.528
0.488PheTrp: 0.488 ± 0.221
1.757PheTyr: 1.757 ± 0.4
0.0PheXaa: 0.0 ± 0.0
Gly
4.394GlyAla: 4.394 ± 0.675
0.195GlyCys: 0.195 ± 0.145
3.222GlyAsp: 3.222 ± 0.59
3.613GlyGlu: 3.613 ± 0.541
3.124GlyPhe: 3.124 ± 0.492
3.808GlyGly: 3.808 ± 0.803
0.976GlyHis: 0.976 ± 0.229
4.687GlyIle: 4.687 ± 0.703
5.761GlyLys: 5.761 ± 0.835
5.37GlyLeu: 5.37 ± 0.754
1.66GlyMet: 1.66 ± 0.502
2.636GlyAsn: 2.636 ± 0.533
0.586GlyPro: 0.586 ± 0.248
3.32GlyGln: 3.32 ± 0.547
3.124GlyArg: 3.124 ± 0.59
2.929GlySer: 2.929 ± 0.642
2.734GlyThr: 2.734 ± 0.471
3.124GlyVal: 3.124 ± 0.646
0.976GlyTrp: 0.976 ± 0.426
3.808GlyTyr: 3.808 ± 0.485
0.0GlyXaa: 0.0 ± 0.0
His
0.781HisAla: 0.781 ± 0.3
0.098HisCys: 0.098 ± 0.087
1.074HisAsp: 1.074 ± 0.283
1.074HisGlu: 1.074 ± 0.318
1.074HisPhe: 1.074 ± 0.301
1.074HisGly: 1.074 ± 0.345
0.293HisHis: 0.293 ± 0.162
1.172HisIle: 1.172 ± 0.321
1.465HisLys: 1.465 ± 0.466
0.683HisLeu: 0.683 ± 0.235
0.391HisMet: 0.391 ± 0.193
1.367HisAsn: 1.367 ± 0.406
0.879HisPro: 0.879 ± 0.296
0.683HisGln: 0.683 ± 0.272
0.195HisArg: 0.195 ± 0.135
0.195HisSer: 0.195 ± 0.144
0.586HisThr: 0.586 ± 0.198
0.781HisVal: 0.781 ± 0.317
0.098HisTrp: 0.098 ± 0.099
0.293HisTyr: 0.293 ± 0.192
0.0HisXaa: 0.0 ± 0.0
Ile
5.565IleAla: 5.565 ± 0.65
0.195IleCys: 0.195 ± 0.141
5.468IleAsp: 5.468 ± 0.868
6.444IleGlu: 6.444 ± 0.894
2.343IlePhe: 2.343 ± 0.423
4.198IleGly: 4.198 ± 0.556
0.879IleHis: 0.879 ± 0.28
4.296IleIle: 4.296 ± 0.554
7.42IleLys: 7.42 ± 0.743
6.542IleLeu: 6.542 ± 0.726
1.757IleMet: 1.757 ± 0.36
3.905IleAsn: 3.905 ± 0.691
2.539IlePro: 2.539 ± 0.496
2.05IleGln: 2.05 ± 0.457
2.246IleArg: 2.246 ± 0.477
5.077IleSer: 5.077 ± 0.761
3.71IleThr: 3.71 ± 0.712
3.905IleVal: 3.905 ± 0.532
1.074IleTrp: 1.074 ± 0.434
2.246IleTyr: 2.246 ± 0.578
0.0IleXaa: 0.0 ± 0.0
Lys
6.249LysAla: 6.249 ± 0.728
0.391LysCys: 0.391 ± 0.188
4.687LysAsp: 4.687 ± 0.722
8.006LysGlu: 8.006 ± 0.732
3.417LysPhe: 3.417 ± 0.575
4.296LysGly: 4.296 ± 0.653
1.465LysHis: 1.465 ± 0.477
6.835LysIle: 6.835 ± 0.883
8.494LysLys: 8.494 ± 1.369
7.713LysLeu: 7.713 ± 1.026
2.05LysMet: 2.05 ± 0.494
4.979LysAsn: 4.979 ± 0.667
2.148LysPro: 2.148 ± 0.426
3.32LysGln: 3.32 ± 0.557
3.613LysArg: 3.613 ± 0.638
5.761LysSer: 5.761 ± 0.871
6.151LysThr: 6.151 ± 0.888
5.565LysVal: 5.565 ± 0.76
0.976LysTrp: 0.976 ± 0.355
2.929LysTyr: 2.929 ± 0.527
0.0LysXaa: 0.0 ± 0.0
Leu
6.835LeuAla: 6.835 ± 0.794
0.879LeuCys: 0.879 ± 0.388
7.616LeuAsp: 7.616 ± 0.794
7.03LeuGlu: 7.03 ± 0.852
2.539LeuPhe: 2.539 ± 0.508
4.394LeuGly: 4.394 ± 0.889
0.879LeuHis: 0.879 ± 0.387
6.346LeuIle: 6.346 ± 0.716
8.397LeuLys: 8.397 ± 1.143
8.104LeuLeu: 8.104 ± 1.186
2.148LeuMet: 2.148 ± 0.5
3.905LeuAsn: 3.905 ± 0.579
2.636LeuPro: 2.636 ± 0.544
3.222LeuGln: 3.222 ± 0.536
3.32LeuArg: 3.32 ± 0.556
6.151LeuSer: 6.151 ± 0.75
5.272LeuThr: 5.272 ± 0.829
5.175LeuVal: 5.175 ± 0.642
0.976LeuTrp: 0.976 ± 0.267
2.246LeuTyr: 2.246 ± 0.472
0.0LeuXaa: 0.0 ± 0.0
Met
1.953MetAla: 1.953 ± 0.377
0.098MetCys: 0.098 ± 0.099
1.855MetAsp: 1.855 ± 0.411
1.953MetGlu: 1.953 ± 0.371
0.683MetPhe: 0.683 ± 0.186
1.465MetGly: 1.465 ± 0.369
0.098MetHis: 0.098 ± 0.096
1.757MetIle: 1.757 ± 0.486
2.05MetLys: 2.05 ± 0.363
2.636MetLeu: 2.636 ± 0.478
0.586MetMet: 0.586 ± 0.198
1.172MetAsn: 1.172 ± 0.394
0.879MetPro: 0.879 ± 0.318
0.488MetGln: 0.488 ± 0.238
1.367MetArg: 1.367 ± 0.367
1.465MetSer: 1.465 ± 0.337
2.148MetThr: 2.148 ± 0.423
2.05MetVal: 2.05 ± 0.477
0.488MetTrp: 0.488 ± 0.28
0.586MetTyr: 0.586 ± 0.232
0.0MetXaa: 0.0 ± 0.0
Asn
4.101AsnAla: 4.101 ± 0.558
0.391AsnCys: 0.391 ± 0.169
3.222AsnAsp: 3.222 ± 0.677
4.491AsnGlu: 4.491 ± 0.617
2.831AsnPhe: 2.831 ± 0.489
5.272AsnGly: 5.272 ± 0.758
1.269AsnHis: 1.269 ± 0.313
4.882AsnIle: 4.882 ± 0.728
4.198AsnLys: 4.198 ± 0.663
4.784AsnLeu: 4.784 ± 0.779
1.66AsnMet: 1.66 ± 0.387
4.003AsnAsn: 4.003 ± 0.548
2.148AsnPro: 2.148 ± 0.574
1.953AsnGln: 1.953 ± 0.471
3.124AsnArg: 3.124 ± 0.611
3.613AsnSer: 3.613 ± 0.435
3.515AsnThr: 3.515 ± 0.482
2.831AsnVal: 2.831 ± 0.497
0.879AsnTrp: 0.879 ± 0.313
2.343AsnTyr: 2.343 ± 0.512
0.0AsnXaa: 0.0 ± 0.0
Pro
1.172ProAla: 1.172 ± 0.333
0.195ProCys: 0.195 ± 0.131
1.757ProAsp: 1.757 ± 0.409
2.831ProGlu: 2.831 ± 0.6
1.172ProPhe: 1.172 ± 0.443
1.66ProGly: 1.66 ± 0.416
0.781ProHis: 0.781 ± 0.257
2.539ProIle: 2.539 ± 0.497
2.831ProLys: 2.831 ± 0.462
1.269ProLeu: 1.269 ± 0.306
0.391ProMet: 0.391 ± 0.19
2.343ProAsn: 2.343 ± 0.469
0.391ProPro: 0.391 ± 0.184
0.488ProGln: 0.488 ± 0.194
0.683ProArg: 0.683 ± 0.259
1.367ProSer: 1.367 ± 0.277
1.367ProThr: 1.367 ± 0.353
2.246ProVal: 2.246 ± 0.539
0.098ProTrp: 0.098 ± 0.084
0.879ProTyr: 0.879 ± 0.28
0.0ProXaa: 0.0 ± 0.0
Gln
2.539GlnAla: 2.539 ± 0.492
0.098GlnCys: 0.098 ± 0.091
1.562GlnAsp: 1.562 ± 0.292
1.953GlnGlu: 1.953 ± 0.488
1.465GlnPhe: 1.465 ± 0.421
1.66GlnGly: 1.66 ± 0.319
0.586GlnHis: 0.586 ± 0.267
2.929GlnIle: 2.929 ± 0.541
3.613GlnLys: 3.613 ± 0.69
3.222GlnLeu: 3.222 ± 0.61
0.781GlnMet: 0.781 ± 0.276
3.417GlnAsn: 3.417 ± 0.614
0.879GlnPro: 0.879 ± 0.274
1.953GlnGln: 1.953 ± 0.516
1.757GlnArg: 1.757 ± 0.437
2.441GlnSer: 2.441 ± 0.44
2.636GlnThr: 2.636 ± 0.407
2.441GlnVal: 2.441 ± 0.418
0.195GlnTrp: 0.195 ± 0.129
1.66GlnTyr: 1.66 ± 0.333
0.0GlnXaa: 0.0 ± 0.0
Arg
1.562ArgAla: 1.562 ± 0.37
0.391ArgCys: 0.391 ± 0.19
2.246ArgAsp: 2.246 ± 0.527
2.831ArgGlu: 2.831 ± 0.718
1.074ArgPhe: 1.074 ± 0.31
2.05ArgGly: 2.05 ± 0.485
0.781ArgHis: 0.781 ± 0.226
2.539ArgIle: 2.539 ± 0.585
3.905ArgLys: 3.905 ± 0.736
5.272ArgLeu: 5.272 ± 1.005
1.757ArgMet: 1.757 ± 0.317
3.124ArgAsn: 3.124 ± 0.543
0.976ArgPro: 0.976 ± 0.266
1.465ArgGln: 1.465 ± 0.405
2.441ArgArg: 2.441 ± 0.717
1.66ArgSer: 1.66 ± 0.404
3.222ArgThr: 3.222 ± 0.644
2.246ArgVal: 2.246 ± 0.588
0.781ArgTrp: 0.781 ± 0.327
1.172ArgTyr: 1.172 ± 0.327
0.0ArgXaa: 0.0 ± 0.0
Ser
3.515SerAla: 3.515 ± 0.624
0.195SerCys: 0.195 ± 0.134
2.929SerAsp: 2.929 ± 0.517
3.905SerGlu: 3.905 ± 0.687
1.855SerPhe: 1.855 ± 0.473
3.027SerGly: 3.027 ± 0.514
0.879SerHis: 0.879 ± 0.266
4.491SerIle: 4.491 ± 0.849
5.468SerLys: 5.468 ± 0.936
6.444SerLeu: 6.444 ± 0.874
1.465SerMet: 1.465 ± 0.362
3.808SerAsn: 3.808 ± 0.492
1.465SerPro: 1.465 ± 0.443
2.441SerGln: 2.441 ± 0.409
2.343SerArg: 2.343 ± 0.497
4.687SerSer: 4.687 ± 0.803
3.515SerThr: 3.515 ± 0.546
3.515SerVal: 3.515 ± 0.606
0.391SerTrp: 0.391 ± 0.147
2.539SerTyr: 2.539 ± 0.489
0.0SerXaa: 0.0 ± 0.0
Thr
4.784ThrAla: 4.784 ± 0.803
0.098ThrCys: 0.098 ± 0.101
3.808ThrAsp: 3.808 ± 0.428
4.784ThrGlu: 4.784 ± 0.736
2.539ThrPhe: 2.539 ± 0.531
5.175ThrGly: 5.175 ± 0.821
0.293ThrHis: 0.293 ± 0.162
4.882ThrIle: 4.882 ± 0.658
4.687ThrLys: 4.687 ± 0.704
6.249ThrLeu: 6.249 ± 0.784
2.05ThrMet: 2.05 ± 0.453
4.101ThrAsn: 4.101 ± 0.57
2.148ThrPro: 2.148 ± 0.468
1.855ThrGln: 1.855 ± 0.436
1.855ThrArg: 1.855 ± 0.465
1.953ThrSer: 1.953 ± 0.487
4.003ThrThr: 4.003 ± 0.641
3.417ThrVal: 3.417 ± 0.668
0.586ThrTrp: 0.586 ± 0.228
2.148ThrTyr: 2.148 ± 0.616
0.0ThrXaa: 0.0 ± 0.0
Val
4.296ValAla: 4.296 ± 0.53
0.195ValCys: 0.195 ± 0.142
4.198ValAsp: 4.198 ± 0.555
4.101ValGlu: 4.101 ± 0.82
2.734ValPhe: 2.734 ± 0.404
4.198ValGly: 4.198 ± 0.827
0.586ValHis: 0.586 ± 0.231
4.101ValIle: 4.101 ± 0.66
5.272ValLys: 5.272 ± 0.76
3.613ValLeu: 3.613 ± 0.528
1.66ValMet: 1.66 ± 0.372
4.198ValAsn: 4.198 ± 0.855
1.367ValPro: 1.367 ± 0.357
1.855ValGln: 1.855 ± 0.378
2.636ValArg: 2.636 ± 0.469
3.222ValSer: 3.222 ± 0.539
4.979ValThr: 4.979 ± 0.737
4.003ValVal: 4.003 ± 0.811
0.488ValTrp: 0.488 ± 0.213
2.246ValTyr: 2.246 ± 0.488
0.0ValXaa: 0.0 ± 0.0
Trp
0.488TrpAla: 0.488 ± 0.198
0.293TrpCys: 0.293 ± 0.158
0.488TrpAsp: 0.488 ± 0.211
1.172TrpGlu: 1.172 ± 0.294
1.074TrpPhe: 1.074 ± 0.31
0.293TrpGly: 0.293 ± 0.151
0.195TrpHis: 0.195 ± 0.147
0.293TrpIle: 0.293 ± 0.152
0.879TrpLys: 0.879 ± 0.294
1.074TrpLeu: 1.074 ± 0.449
0.293TrpMet: 0.293 ± 0.166
0.586TrpAsn: 0.586 ± 0.36
0.195TrpPro: 0.195 ± 0.118
0.683TrpGln: 0.683 ± 0.288
0.683TrpArg: 0.683 ± 0.233
0.879TrpSer: 0.879 ± 0.34
0.488TrpThr: 0.488 ± 0.178
0.879TrpVal: 0.879 ± 0.296
0.293TrpTrp: 0.293 ± 0.223
0.683TrpTyr: 0.683 ± 0.506
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.441TyrAla: 2.441 ± 0.528
0.195TyrCys: 0.195 ± 0.13
2.831TyrAsp: 2.831 ± 0.44
2.246TyrGlu: 2.246 ± 0.53
2.636TyrPhe: 2.636 ± 0.505
1.66TyrGly: 1.66 ± 0.514
0.781TyrHis: 0.781 ± 0.28
1.855TyrIle: 1.855 ± 0.488
4.198TyrLys: 4.198 ± 0.591
3.222TyrLeu: 3.222 ± 0.587
0.879TyrMet: 0.879 ± 0.312
2.246TyrAsn: 2.246 ± 0.442
1.66TyrPro: 1.66 ± 0.423
2.05TyrGln: 2.05 ± 0.436
2.05TyrArg: 2.05 ± 0.493
2.343TyrSer: 2.343 ± 0.539
1.855TyrThr: 1.855 ± 0.479
1.66TyrVal: 1.66 ± 0.43
0.293TyrTrp: 0.293 ± 0.164
1.855TyrTyr: 1.855 ± 0.635
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (10243 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski