Candidatus Phycorickettsia trachydisci

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Rickettsiaceae; Candidatus Phycorickettsia

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1P988|A0A2P1P988_9RICK tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase OS=Candidatus Phycorickettsia trachydisci OX=2115978 GN=miaB PE=3 SV=1
MM1 pKa = 7.39QNITNMEE8 pKa = 4.26EE9 pKa = 4.19TNKK12 pKa = 9.76IPAKK16 pKa = 10.55LYY18 pKa = 10.77DD19 pKa = 4.44KK20 pKa = 10.49YY21 pKa = 11.03HH22 pKa = 5.85QSQYY26 pKa = 11.26SADD29 pKa = 3.72DD30 pKa = 3.77LEE32 pKa = 5.72IYY34 pKa = 8.76STTVEE39 pKa = 3.99ITGDD43 pKa = 3.49DD44 pKa = 3.39TDD46 pKa = 3.54

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1P965|A0A2P1P965_9RICK Uncharacterized protein OS=Candidatus Phycorickettsia trachydisci OX=2115978 GN=phytr_8920 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.27FMKK5 pKa = 9.54RR6 pKa = 11.84TYY8 pKa = 10.09QPSKK12 pKa = 9.73LVRR15 pKa = 11.84KK16 pKa = 9.15RR17 pKa = 11.84RR18 pKa = 11.84HH19 pKa = 4.42GFRR22 pKa = 11.84ARR24 pKa = 11.84MATAGGRR31 pKa = 11.84AILNRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.7KK40 pKa = 9.85GRR42 pKa = 11.84KK43 pKa = 8.66VLSAA47 pKa = 4.05

Molecular weight: 5.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1244

0

1244

442354

31

4901

355.6

40.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.708 ± 0.1

1.141 ± 0.028

5.69 ± 0.051

6.271 ± 0.079

4.564 ± 0.073

5.661 ± 0.082

2.125 ± 0.04

8.68 ± 0.09

8.258 ± 0.071

10.358 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.165 ± 0.034

5.752 ± 0.054

3.406 ± 0.062

3.821 ± 0.056

3.435 ± 0.06

7.135 ± 0.069

4.807 ± 0.076

5.598 ± 0.057

0.81 ± 0.022

3.617 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski