Mycoplasma wenyonii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 629 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328PJL0|A0A328PJL0_9MOLU 30S ribosomal protein S13 OS=Mycoplasma wenyonii OX=65123 GN=rpsM PE=3 SV=1
MM1 pKa = 7.61CPEE4 pKa = 4.01QSLQQLPQDD13 pKa = 3.55SSSAQKK19 pKa = 10.42SEE21 pKa = 3.88QQLEE25 pKa = 4.48VVEE28 pKa = 4.26QQWWWWEE35 pKa = 3.79WQQEE39 pKa = 4.31DD40 pKa = 4.02IQQQEE45 pKa = 4.2PQQWWHH51 pKa = 6.49ICLQQQDD58 pKa = 3.08IDD60 pKa = 4.1LPP62 pKa = 4.18

Molecular weight: 7.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328PJF4|A0A328PJF4_9MOLU Tryptophan--tRNA ligase OS=Mycoplasma wenyonii OX=65123 GN=trpS PE=3 SV=1
MM1 pKa = 7.32AVSPRR6 pKa = 11.84RR7 pKa = 11.84VSKK10 pKa = 10.51SRR12 pKa = 11.84KK13 pKa = 8.25GMRR16 pKa = 11.84NSHH19 pKa = 6.25AGIYY23 pKa = 10.32SLAPLMKK30 pKa = 10.02CPKK33 pKa = 9.63CAEE36 pKa = 4.0QVRR39 pKa = 11.84LHH41 pKa = 7.07RR42 pKa = 11.84ICKK45 pKa = 9.51CGQYY49 pKa = 10.12RR50 pKa = 11.84NKK52 pKa = 10.63KK53 pKa = 9.3IFF55 pKa = 3.45

Molecular weight: 6.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

629

0

629

174754

27

2655

277.8

31.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.387 ± 0.072

1.209 ± 0.033

4.501 ± 0.071

7.82 ± 0.105

5.361 ± 0.088

5.631 ± 0.097

1.489 ± 0.034

7.2 ± 0.077

9.669 ± 0.101

10.757 ± 0.135

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.421 ± 0.03

5.345 ± 0.062

3.197 ± 0.051

3.861 ± 0.064

3.812 ± 0.063

8.631 ± 0.117

5.235 ± 0.078

5.242 ± 0.079

1.595 ± 0.045

3.637 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski