Streptococcus satellite phage Javan529

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZQ16|A0A4D5ZQ16_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan529 OX=2558730 GN=JavanS529_0028 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84TFSDD6 pKa = 3.68TPKK9 pKa = 10.71QFMFTYY15 pKa = 9.75QCKK18 pKa = 10.39DD19 pKa = 2.96YY20 pKa = 9.71DD21 pKa = 4.03TARR24 pKa = 11.84VTSTAILGYY33 pKa = 8.7ITGTYY38 pKa = 7.79EE39 pKa = 3.86QNLAEE44 pKa = 4.27ATLNGDD50 pKa = 3.7GYY52 pKa = 11.66LEE54 pKa = 3.88VTYY57 pKa = 10.94FEE59 pKa = 5.3DD60 pKa = 4.58KK61 pKa = 10.97SINFNLKK68 pKa = 10.17RR69 pKa = 11.84ICDD72 pKa = 3.8SFKK75 pKa = 10.88DD76 pKa = 3.81YY77 pKa = 11.31CNQPEE82 pKa = 4.1DD83 pKa = 3.82MEE85 pKa = 4.59GEE87 pKa = 4.27KK88 pKa = 10.75

Molecular weight: 10.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZQJ6|A0A4D5ZQJ6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan529 OX=2558730 GN=JavanS529_0010 PE=4 SV=1
MM1 pKa = 7.49AGSFRR6 pKa = 11.84QHH8 pKa = 6.21HH9 pKa = 6.18PAFDD13 pKa = 3.68NMLLADD19 pKa = 3.8DD20 pKa = 5.86RR21 pKa = 11.84IYY23 pKa = 11.22KK24 pKa = 9.5NRR26 pKa = 11.84RR27 pKa = 11.84PTVEE31 pKa = 3.82QNKK34 pKa = 8.53EE35 pKa = 3.78QQHH38 pKa = 5.43LKK40 pKa = 10.3KK41 pKa = 10.5LKK43 pKa = 8.78KK44 pKa = 9.76KK45 pKa = 9.78RR46 pKa = 11.84RR47 pKa = 11.84KK48 pKa = 9.22GQQ50 pKa = 3.06

Molecular weight: 6.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30

0

30

3672

37

562

122.4

14.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.739 ± 0.49

0.681 ± 0.228

5.746 ± 0.368

8.143 ± 0.581

4.521 ± 0.339

4.684 ± 0.436

1.797 ± 0.225

6.944 ± 0.493

9.777 ± 0.535

10.022 ± 0.631

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.478 ± 0.257

5.256 ± 0.365

3.077 ± 0.442

4.766 ± 0.33

4.902 ± 0.343

5.692 ± 0.336

5.855 ± 0.5

5.011 ± 0.452

0.953 ± 0.175

4.956 ± 0.397
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski