Cestrum yellow leaf curling virus (CmYLCV)
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q7TD13|MVP_CYLCV Putative movement protein OS=Cestrum yellow leaf curling virus OX=175814 GN=ORF I PE=3 SV=1
MM1 pKa = 6.71 SQEE4 pKa = 4.25 RR5 pKa = 11.84 VDD7 pKa = 3.7 KK8 pKa = 10.8 LRR10 pKa = 11.84 KK11 pKa = 9.51 LRR13 pKa = 11.84 IEE15 pKa = 4.01 FEE17 pKa = 4.48 GIIQRR22 pKa = 11.84 MEE24 pKa = 3.75 VAKK27 pKa = 10.84 NLLRR31 pKa = 11.84 EE32 pKa = 4.35 LEE34 pKa = 4.19 EE35 pKa = 3.88 TTDD38 pKa = 3.25 RR39 pKa = 11.84 VRR41 pKa = 11.84 VDD43 pKa = 3.51 RR44 pKa = 11.84 LQMEE48 pKa = 4.5 VGFLRR53 pKa = 11.84 DD54 pKa = 3.5 YY55 pKa = 11.94 ADD57 pKa = 3.63 INCMIEE63 pKa = 3.8 KK64 pKa = 10.47 DD65 pKa = 3.75 RR66 pKa = 11.84 IKK68 pKa = 10.28 EE69 pKa = 3.95 QSSSSSATRR78 pKa = 11.84 TTQEE82 pKa = 4.2 PSLHH86 pKa = 6.6 LPDD89 pKa = 3.98
Molecular weight: 10.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.411
IPC2_protein 5.512
IPC_protein 5.359
Toseland 5.308
ProMoST 5.219
Dawson 5.334
Bjellqvist 5.486
Wikipedia 5.194
Rodwell 5.258
Grimsley 5.232
Solomon 5.334
Lehninger 5.296
Nozaki 5.461
DTASelect 5.588
Thurlkill 5.308
EMBOSS 5.245
Sillero 5.537
Patrickios 4.774
IPC_peptide 5.347
IPC2_peptide 5.537
IPC2.peptide.svr19 5.72
Protein with the highest isoelectric point:
>sp|Q7TD09|CAPSD_CYLCV Capsid protein OS=Cestrum yellow leaf curling virus OX=175814 GN=ORF IV PE=3 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.69 SKK4 pKa = 10.84 GNPNATFITVKK15 pKa = 10.51 INDD18 pKa = 3.78 VFINAYY24 pKa = 9.51 VDD26 pKa = 3.57 TGATICLADD35 pKa = 4.29 PKK37 pKa = 10.8 IKK39 pKa = 10.7 LKK41 pKa = 8.32 WVKK44 pKa = 8.92 MEE46 pKa = 4.13 KK47 pKa = 9.85 PIKK50 pKa = 10.15 ISIADD55 pKa = 3.65 KK56 pKa = 9.91 SVQEE60 pKa = 3.63 IWHH63 pKa = 5.86 RR64 pKa = 11.84 AEE66 pKa = 4.6 MVEE69 pKa = 3.63 IWIRR73 pKa = 11.84 NYY75 pKa = 10.62 KK76 pKa = 9.68 FVAATVCQKK85 pKa = 10.91 SSGMDD90 pKa = 3.02 FVIGNNFLRR99 pKa = 11.84 LYY101 pKa = 10.62 QPFIQGLNYY110 pKa = 9.78 IKK112 pKa = 10.66 LRR114 pKa = 11.84 APLDD118 pKa = 3.44 KK119 pKa = 10.91 DD120 pKa = 3.18 INQPSKK126 pKa = 10.34 MIYY129 pKa = 9.97 IPVTTPSKK137 pKa = 10.18 ILQFAILEE145 pKa = 4.15 KK146 pKa = 10.78 LQDD149 pKa = 3.57 ILFEE153 pKa = 4.12 LHH155 pKa = 5.48 VQEE158 pKa = 4.74 NSKK161 pKa = 9.08 TPLEE165 pKa = 4.38 LKK167 pKa = 10.58 VSSTLEE173 pKa = 4.09 EE174 pKa = 4.27 VCDD177 pKa = 4.13 EE178 pKa = 4.5 NPLDD182 pKa = 3.88 VKK184 pKa = 10.04 NTNTEE189 pKa = 3.87 LVKK192 pKa = 10.44 IEE194 pKa = 4.91 LINPEE199 pKa = 4.19 KK200 pKa = 10.46 EE201 pKa = 4.11 VNVPNNIPYY210 pKa = 10.29 SLRR213 pKa = 11.84 DD214 pKa = 3.34 INEE217 pKa = 4.23 FSQEE221 pKa = 3.98 CADD224 pKa = 4.17 LVRR227 pKa = 11.84 KK228 pKa = 9.91 GIIEE232 pKa = 4.08 EE233 pKa = 4.41 SKK235 pKa = 10.75 SPHH238 pKa = 6.11 SAPAFYY244 pKa = 10.93 VEE246 pKa = 3.96 NHH248 pKa = 5.87 NEE250 pKa = 3.65 IKK252 pKa = 10.1 RR253 pKa = 11.84 KK254 pKa = 8.33 KK255 pKa = 8.87 RR256 pKa = 11.84 RR257 pKa = 11.84 MVINYY262 pKa = 7.65 KK263 pKa = 10.32 ALNKK267 pKa = 9.17 ATIGNAHH274 pKa = 7.33 KK275 pKa = 10.52 LPRR278 pKa = 11.84 IDD280 pKa = 5.46 SILTKK285 pKa = 10.75 VKK287 pKa = 10.01 GSNWFSTLDD296 pKa = 3.37 AKK298 pKa = 10.57 SGYY301 pKa = 7.83 WQLRR305 pKa = 11.84 LHH307 pKa = 6.32 PQSKK311 pKa = 9.67 PLTAFSCPPQKK322 pKa = 10.42 HH323 pKa = 4.95 YY324 pKa = 10.35 QWNVLPFGLKK334 pKa = 8.94 QAPGIYY340 pKa = 9.8 QNFMDD345 pKa = 5.07 KK346 pKa = 10.78 NLEE349 pKa = 3.97 GLEE352 pKa = 4.29 NFCLAYY358 pKa = 9.76 IDD360 pKa = 5.6 DD361 pKa = 4.18 ILVFTNSSRR370 pKa = 11.84 EE371 pKa = 3.86 EE372 pKa = 4.13 HH373 pKa = 6.21 LSKK376 pKa = 10.98 LLVVLEE382 pKa = 4.09 RR383 pKa = 11.84 CKK385 pKa = 10.93 EE386 pKa = 3.8 KK387 pKa = 11.11 GLILSKK393 pKa = 10.56 KK394 pKa = 8.55 KK395 pKa = 10.74 AIIARR400 pKa = 11.84 QTIDD404 pKa = 3.73 FLGLTLQEE412 pKa = 4.1 NGEE415 pKa = 4.31 IKK417 pKa = 10.47 LQPNVLEE424 pKa = 4.28 KK425 pKa = 11.42 LEE427 pKa = 4.6 LFPDD431 pKa = 4.64 AIEE434 pKa = 5.46 DD435 pKa = 3.63 RR436 pKa = 11.84 KK437 pKa = 10.26 QLQRR441 pKa = 11.84 FLGCLNYY448 pKa = 9.98 IADD451 pKa = 3.78 KK452 pKa = 11.38 GFLKK456 pKa = 10.68 EE457 pKa = 3.8 IAKK460 pKa = 7.49 EE461 pKa = 4.3 TKK463 pKa = 9.51 NLYY466 pKa = 9.85 PKK468 pKa = 10.78 VSITNPWHH476 pKa = 6.39 WSDD479 pKa = 5.14 LDD481 pKa = 3.93 SKK483 pKa = 11.07 LVNQIKK489 pKa = 10.27 KK490 pKa = 9.43 KK491 pKa = 10.83 CKK493 pKa = 9.91 DD494 pKa = 3.46 LSPLYY499 pKa = 10.05 FPKK502 pKa = 10.71 PEE504 pKa = 5.05 DD505 pKa = 3.52 YY506 pKa = 11.22 LIIEE510 pKa = 4.65 TDD512 pKa = 3.18 ASGDD516 pKa = 3.26 TWAGCLKK523 pKa = 10.42 AAEE526 pKa = 4.35 LLFPKK531 pKa = 9.43 GTKK534 pKa = 9.7 NKK536 pKa = 9.81 VVEE539 pKa = 4.38 RR540 pKa = 11.84 LCKK543 pKa = 7.76 YY544 pKa = 8.67 TSGIFSSAEE553 pKa = 3.51 QKK555 pKa = 9.33 YY556 pKa = 7.05 TVHH559 pKa = 6.39 EE560 pKa = 4.52 KK561 pKa = 9.01 EE562 pKa = 4.01 TLAALKK568 pKa = 9.71 TMRR571 pKa = 11.84 KK572 pKa = 7.89 WKK574 pKa = 10.82 AEE576 pKa = 3.98 LLPKK580 pKa = 10.42 EE581 pKa = 4.03 FTLRR585 pKa = 11.84 TDD587 pKa = 2.87 SSYY590 pKa = 8.73 VTGFARR596 pKa = 11.84 HH597 pKa = 5.41 NLKK600 pKa = 10.81 ANYY603 pKa = 9.1 NQGRR607 pKa = 11.84 LVRR610 pKa = 11.84 WQLEE614 pKa = 3.95 FLQYY618 pKa = 9.13 PARR621 pKa = 11.84 VEE623 pKa = 4.16 YY624 pKa = 10.46 IKK626 pKa = 11.13 GEE628 pKa = 4.27 KK629 pKa = 10.01 NSLADD634 pKa = 3.34 TLTRR638 pKa = 11.84 EE639 pKa = 4.59 WKK641 pKa = 9.22 QQQ643 pKa = 3.03
Molecular weight: 74.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.576
IPC2_protein 8.448
IPC_protein 8.287
Toseland 9.107
ProMoST 8.887
Dawson 9.399
Bjellqvist 9.18
Wikipedia 9.516
Rodwell 9.721
Grimsley 9.414
Solomon 9.428
Lehninger 9.385
Nozaki 9.37
DTASelect 9.092
Thurlkill 9.282
EMBOSS 9.56
Sillero 9.414
Patrickios 4.889
IPC_peptide 9.414
IPC2_peptide 7.732
IPC2.peptide.svr19 7.727
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2455
89
643
350.7
40.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.807 ± 0.592
1.711 ± 0.34
4.888 ± 0.463
9.328 ± 0.839
4.196 ± 0.29
4.033 ± 0.286
1.548 ± 0.3
7.617 ± 0.411
11.039 ± 0.511
8.88 ± 1.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.2 ± 0.486
5.092 ± 0.515
4.684 ± 0.343
4.644 ± 0.427
4.236 ± 0.561
7.291 ± 0.819
5.092 ± 0.174
4.562 ± 0.586
1.018 ± 0.304
3.136 ± 0.483
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here