Mycolicibacterium neoaurum VKM Ac-1815D

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycolicibacterium; Mycolicibacterium neoaurum

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4995 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5XAL3|V5XAL3_MYCNE MerR family transcriptional regulator OS=Mycolicibacterium neoaurum VKM Ac-1815D OX=700508 GN=D174_07550 PE=4 SV=1
MM1 pKa = 7.98RR2 pKa = 11.84DD3 pKa = 3.99DD4 pKa = 5.41LPPGLPPDD12 pKa = 4.75PFADD16 pKa = 4.31DD17 pKa = 4.42PSDD20 pKa = 3.64PSAALDD26 pKa = 4.5AIEE29 pKa = 5.41PGLPLDD35 pKa = 3.93PQEE38 pKa = 4.44RR39 pKa = 11.84SAVEE43 pKa = 3.96ADD45 pKa = 3.59LADD48 pKa = 3.99LAVYY52 pKa = 8.4EE53 pKa = 4.77ALLAHH58 pKa = 6.71KK59 pKa = 9.46GIRR62 pKa = 11.84GLVVCCDD69 pKa = 4.16DD70 pKa = 3.98CQQDD74 pKa = 4.42HH75 pKa = 5.31YY76 pKa = 11.02HH77 pKa = 6.72DD78 pKa = 4.0WDD80 pKa = 3.57MLRR83 pKa = 11.84ANLLQLLVDD92 pKa = 4.02GTVRR96 pKa = 11.84PHH98 pKa = 6.27EE99 pKa = 4.31PAYY102 pKa = 10.66DD103 pKa = 4.13PEE105 pKa = 6.04PDD107 pKa = 5.08AYY109 pKa = 9.99VTWDD113 pKa = 3.35YY114 pKa = 11.62CRR116 pKa = 11.84GYY118 pKa = 11.49ADD120 pKa = 4.25ASLNEE125 pKa = 4.24ATTEE129 pKa = 3.89HH130 pKa = 7.27DD131 pKa = 3.64GFRR134 pKa = 4.1

Molecular weight: 14.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5XJF9|V5XJF9_MYCNE Uncharacterized protein OS=Mycolicibacterium neoaurum VKM Ac-1815D OX=700508 GN=D174_24130 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84LCRR5 pKa = 11.84LPVVPVARR13 pKa = 11.84ARR15 pKa = 11.84PVVAQVVPARR25 pKa = 11.84LVAVRR30 pKa = 11.84APVVPVLRR38 pKa = 11.84RR39 pKa = 11.84LQSVVRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84VVLPVVVRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84VVLPVAALLPVVLPVVALRR76 pKa = 11.84SVVALPWVVAPRR88 pKa = 11.84WVAVLPWVVAPRR100 pKa = 11.84WVAVLPWVVAPPP112 pKa = 3.53

Molecular weight: 12.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4995

0

4995

1627888

28

5015

325.9

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.222 ± 0.049

0.769 ± 0.009

6.392 ± 0.029

5.147 ± 0.032

3.041 ± 0.02

8.97 ± 0.026

2.163 ± 0.016

4.454 ± 0.023

2.073 ± 0.022

9.927 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.023 ± 0.014

2.187 ± 0.017

5.726 ± 0.03

2.965 ± 0.02

7.071 ± 0.035

5.343 ± 0.021

6.23 ± 0.027

8.72 ± 0.031

1.464 ± 0.015

2.111 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski