Sphaeropsis sapinea RNA virus 1
Average proteome isoelectric point is 7.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9YXE7|Q9YXE7_9VIRU Coat protein OS=Sphaeropsis sapinea RNA virus 1 OX=73497 PE=4 SV=1
MM1 pKa = 7.65 PPISTAQWYY10 pKa = 9.35 HH11 pKa = 5.63 SLLLKK16 pKa = 10.75 SLFVNRR22 pKa = 11.84 FRR24 pKa = 11.84 QSSLFGGRR32 pKa = 11.84 VPLIPNTTIDD42 pKa = 3.95 GYY44 pKa = 9.62 ITTHH48 pKa = 5.19 QHH50 pKa = 4.68 HH51 pKa = 6.07 TTPNFPMEE59 pKa = 4.19 HH60 pKa = 6.88 ALRR63 pKa = 11.84 NAFLAAVIASPRR75 pKa = 11.84 GGTLLTAPTKK85 pKa = 10.3 KK86 pKa = 10.44 GKK88 pKa = 8.93 EE89 pKa = 3.95 KK90 pKa = 10.42 KK91 pKa = 10.43 APLATYY97 pKa = 9.71 RR98 pKa = 11.84 RR99 pKa = 11.84 YY100 pKa = 9.78 RR101 pKa = 11.84 ANIRR105 pKa = 11.84 TEE107 pKa = 3.77 STIAGNVDD115 pKa = 2.82 ARR117 pKa = 11.84 VAGIQYY123 pKa = 9.82 EE124 pKa = 3.76 IGARR128 pKa = 11.84 YY129 pKa = 9.07 EE130 pKa = 4.09 RR131 pKa = 11.84 VGEE134 pKa = 4.02 AFAPVPEE141 pKa = 4.13 EE142 pKa = 3.66 QVLFEE147 pKa = 4.08 AAYY150 pKa = 9.16 PSSAALAEE158 pKa = 4.36 DD159 pKa = 3.91 FVGFARR165 pKa = 11.84 KK166 pKa = 9.52 YY167 pKa = 9.7 SNFSATFAHH176 pKa = 6.78 SSLAGLVEE184 pKa = 4.46 RR185 pKa = 11.84 VARR188 pKa = 11.84 TLGALTVFPSGTFDD202 pKa = 3.05 QDD204 pKa = 3.55 AIRR207 pKa = 11.84 GGRR210 pKa = 11.84 PLMIAALGTLDD221 pKa = 4.52 GPVNSLAGSVFIPRR235 pKa = 11.84 LVDD238 pKa = 3.7 SVISPDD244 pKa = 3.37 VFTILINAAAGEE256 pKa = 4.37 GSRR259 pKa = 11.84 VITDD263 pKa = 3.5 VLEE266 pKa = 4.52 LDD268 pKa = 3.27 ATTRR272 pKa = 11.84 RR273 pKa = 11.84 PIVPTLRR280 pKa = 11.84 DD281 pKa = 3.22 SSMLLPCVEE290 pKa = 4.4 ALRR293 pKa = 11.84 ILGANMAACDD303 pKa = 3.93 QGPLFAFALVRR314 pKa = 11.84 GLNAVLSLVGHH325 pKa = 5.75 TDD327 pKa = 3.14 EE328 pKa = 6.4 AGVTRR333 pKa = 11.84 DD334 pKa = 2.94 IFRR337 pKa = 11.84 VSGFDD342 pKa = 3.09 IPFGGIHH349 pKa = 6.86 FGLEE353 pKa = 4.17 PYY355 pKa = 10.67 AGLPAVASNAGADD368 pKa = 3.41 ACCYY372 pKa = 10.64 VDD374 pKa = 4.53 ALLMTSGALVAHH386 pKa = 7.19 CDD388 pKa = 3.33 PGQEE392 pKa = 4.04 YY393 pKa = 10.38 GGRR396 pKa = 11.84 WFPTVLQGTGPDD408 pKa = 3.53 TAEE411 pKa = 3.82 VRR413 pKa = 11.84 PGQSQEE419 pKa = 3.79 GTADD423 pKa = 3.12 MANRR427 pKa = 11.84 NRR429 pKa = 11.84 GLLASSMPRR438 pKa = 11.84 FAEE441 pKa = 4.62 HH442 pKa = 6.27 YY443 pKa = 10.31 ARR445 pKa = 11.84 GLGRR449 pKa = 11.84 LFAIEE454 pKa = 3.69 GDD456 pKa = 3.28 ARR458 pKa = 11.84 IVVNILSASARR469 pKa = 11.84 LLPDD473 pKa = 2.89 NCRR476 pKa = 11.84 HH477 pKa = 5.59 LRR479 pKa = 11.84 YY480 pKa = 9.33 PSVSPYY486 pKa = 10.22 FWVEE490 pKa = 3.85 PTSLLPPDD498 pKa = 4.44 FLGTAAEE505 pKa = 4.26 LNGCGSLAMRR515 pKa = 11.84 GTSRR519 pKa = 11.84 TRR521 pKa = 11.84 QAWEE525 pKa = 4.36 DD526 pKa = 3.34 IEE528 pKa = 5.0 RR529 pKa = 11.84 VGDD532 pKa = 3.58 EE533 pKa = 4.47 DD534 pKa = 4.53 VTFTGYY540 pKa = 10.15 NVAMPFARR548 pKa = 11.84 SSWFLIHH555 pKa = 6.88 WLNHH559 pKa = 5.39 PANGLGALRR568 pKa = 11.84 VRR570 pKa = 11.84 QLDD573 pKa = 3.84 PNGVIHH579 pKa = 7.1 PGPCADD585 pKa = 4.09 NPDD588 pKa = 3.12 VRR590 pKa = 11.84 DD591 pKa = 3.44 RR592 pKa = 11.84 VEE594 pKa = 4.02 ASLPITDD601 pKa = 4.11 YY602 pKa = 11.27 LWVRR606 pKa = 11.84 GQSPFPAPGEE616 pKa = 3.96 FLNLTGTLGLLARR629 pKa = 11.84 HH630 pKa = 5.93 LTMDD634 pKa = 4.71 DD635 pKa = 3.69 DD636 pKa = 5.37 GIPTEE641 pKa = 4.03 EE642 pKa = 4.26 HH643 pKa = 6.72 LPTGRR648 pKa = 11.84 EE649 pKa = 3.78 FRR651 pKa = 11.84 DD652 pKa = 3.37 TAVTVVAGRR661 pKa = 11.84 PVGLPNGAHH670 pKa = 6.39 NAYY673 pKa = 10.26 DD674 pKa = 3.66 SQARR678 pKa = 11.84 RR679 pKa = 11.84 ARR681 pKa = 11.84 TRR683 pKa = 11.84 ATRR686 pKa = 11.84 EE687 pKa = 3.73 LAASAARR694 pKa = 11.84 ARR696 pKa = 11.84 LYY698 pKa = 11.07 GRR700 pKa = 11.84 ATVAEE705 pKa = 4.27 MPILTSAPVLRR716 pKa = 11.84 AAPPRR721 pKa = 11.84 PEE723 pKa = 4.19 AVNPMEE729 pKa = 4.98 GGGPGHH735 pKa = 7.34 DD736 pKa = 4.0 LARR739 pKa = 11.84 HH740 pKa = 5.42 SGMGRR745 pKa = 11.84 HH746 pKa = 5.96 TYY748 pKa = 10.23 PPDD751 pKa = 3.53 RR752 pKa = 11.84 EE753 pKa = 4.28 QRR755 pKa = 11.84 GDD757 pKa = 3.43 PAVPVPQHH765 pKa = 4.83 QALRR769 pKa = 11.84 APQMPRR775 pKa = 11.84 PGPNPQGGGVIPPPPPLPPSGGGDD799 pKa = 3.33 GSGPSSGGSCAQEE812 pKa = 4.32 DD813 pKa = 4.8 TNPAPPVAPGPAQPDD828 pKa = 4.06 GPANEE833 pKa = 4.18
Molecular weight: 88.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.123
IPC2_protein 6.008
IPC_protein 6.109
Toseland 6.504
ProMoST 6.427
Dawson 6.427
Bjellqvist 6.415
Wikipedia 6.453
Rodwell 6.415
Grimsley 6.664
Solomon 6.427
Lehninger 6.427
Nozaki 6.707
DTASelect 6.898
Thurlkill 6.912
EMBOSS 6.883
Sillero 6.81
Patrickios 4.342
IPC_peptide 6.44
IPC2_peptide 6.81
IPC2.peptide.svr19 6.704
Protein with the highest isoelectric point:
>tr|Q9YXE7|Q9YXE7_9VIRU Coat protein OS=Sphaeropsis sapinea RNA virus 1 OX=73497 PE=4 SV=1
MM1 pKa = 7.45 NKK3 pKa = 10.13 FIDD6 pKa = 3.68 RR7 pKa = 11.84 AEE9 pKa = 4.01 QFGPVGDD16 pKa = 4.13 YY17 pKa = 10.88 LLSVVEE23 pKa = 4.5 RR24 pKa = 11.84 FPTVHH29 pKa = 6.84 SYY31 pKa = 11.91 GEE33 pKa = 4.07 LSFVDD38 pKa = 3.17 ALTRR42 pKa = 11.84 LRR44 pKa = 11.84 AMSNNLHH51 pKa = 6.81 ANDD54 pKa = 4.67 ALLPAAVSLLCLPFPLQVEE73 pKa = 4.51 MSRR76 pKa = 11.84 ADD78 pKa = 3.55 VYY80 pKa = 11.78 ALLVKK85 pKa = 9.59 GTSLPFNFVRR95 pKa = 11.84 STAHH99 pKa = 6.63 LHH101 pKa = 6.01 PLPDD105 pKa = 3.81 DD106 pKa = 3.65 VGRR109 pKa = 11.84 PGSTRR114 pKa = 11.84 YY115 pKa = 9.69 SGRR118 pKa = 11.84 FFARR122 pKa = 11.84 LLNDD126 pKa = 2.99 HH127 pKa = 6.29 SFRR130 pKa = 11.84 RR131 pKa = 11.84 SCFPTKK137 pKa = 10.58 KK138 pKa = 9.53 MLPAEE143 pKa = 4.35 VKK145 pKa = 10.36 PNLALAPLIRR155 pKa = 11.84 SFSTCAGPTLAGHH168 pKa = 6.73 FLRR171 pKa = 11.84 HH172 pKa = 5.67 LAGHH176 pKa = 4.86 VTEE179 pKa = 5.02 DD180 pKa = 2.97 VAMAAMVYY188 pKa = 10.54 AHH190 pKa = 7.0 GLVACYY196 pKa = 9.34 GQKK199 pKa = 10.76 GYY201 pKa = 10.72 DD202 pKa = 3.01 IAAWLVLRR210 pKa = 11.84 PAAAKK215 pKa = 10.45 GISTAIKK222 pKa = 9.99 ALGANAHH229 pKa = 6.67 DD230 pKa = 5.18 FGAVLCEE237 pKa = 3.71 AQTLLGRR244 pKa = 11.84 AVSTIDD250 pKa = 3.43 LAHH253 pKa = 6.03 EE254 pKa = 4.08 AEE256 pKa = 4.52 YY257 pKa = 10.9 RR258 pKa = 11.84 CDD260 pKa = 3.74 PDD262 pKa = 5.2 LVAKK266 pKa = 9.51 QVIEE270 pKa = 4.23 PGEE273 pKa = 4.04 EE274 pKa = 3.88 LRR276 pKa = 11.84 SHH278 pKa = 6.03 IRR280 pKa = 11.84 AVLAMEE286 pKa = 4.5 LAGRR290 pKa = 11.84 DD291 pKa = 3.62 LSLPDD296 pKa = 4.28 LDD298 pKa = 4.68 SWWSSRR304 pKa = 11.84 WLWCVNGSQNDD315 pKa = 3.4 ASSRR319 pKa = 11.84 LLGIDD324 pKa = 3.01 TARR327 pKa = 11.84 FRR329 pKa = 11.84 EE330 pKa = 4.13 FHH332 pKa = 5.07 TRR334 pKa = 11.84 EE335 pKa = 3.9 YY336 pKa = 11.11 RR337 pKa = 11.84 RR338 pKa = 11.84 MASEE342 pKa = 4.4 ALTHH346 pKa = 6.23 EE347 pKa = 5.76 PITSWDD353 pKa = 3.49 GYY355 pKa = 7.88 TNISASPKK363 pKa = 9.75 LEE365 pKa = 4.18 HH366 pKa = 6.54 GKK368 pKa = 7.98 TRR370 pKa = 11.84 AIFACDD376 pKa = 3.1 TRR378 pKa = 11.84 SYY380 pKa = 11.07 FAFEE384 pKa = 4.1 WLLGATQKK392 pKa = 10.68 AWRR395 pKa = 11.84 NHH397 pKa = 5.39 RR398 pKa = 11.84 VLLDD402 pKa = 3.81 PGGGGHH408 pKa = 7.34 LGISRR413 pKa = 11.84 RR414 pKa = 11.84 VRR416 pKa = 11.84 SFMKK420 pKa = 10.36 HH421 pKa = 5.01 GGVNLMLDD429 pKa = 3.53 FDD431 pKa = 5.41 DD432 pKa = 5.75 FNSHH436 pKa = 6.89 HH437 pKa = 6.62 SLNSQRR443 pKa = 11.84 MLFGEE448 pKa = 4.29 LCDD451 pKa = 3.95 RR452 pKa = 11.84 ANAPGWYY459 pKa = 9.23 RR460 pKa = 11.84 KK461 pKa = 9.7 VLSDD465 pKa = 3.05 SWGKK469 pKa = 7.92 MHH471 pKa = 7.04 VNIAGHH477 pKa = 4.98 MRR479 pKa = 11.84 PWLGTLPSGHH489 pKa = 7.16 RR490 pKa = 11.84 GTTIVNSVLNAAYY503 pKa = 9.67 IRR505 pKa = 11.84 MALGGPAFDD514 pKa = 5.23 KK515 pKa = 10.34 LTSLHH520 pKa = 6.47 TGDD523 pKa = 4.85 DD524 pKa = 3.79 VYY526 pKa = 11.3 IRR528 pKa = 11.84 ADD530 pKa = 3.4 TLTSCEE536 pKa = 4.21 WILDD540 pKa = 3.61 RR541 pKa = 11.84 VRR543 pKa = 11.84 SVGCRR548 pKa = 11.84 INPAKK553 pKa = 10.39 QSVGFGTGEE562 pKa = 4.26 FLRR565 pKa = 11.84 MAITQRR571 pKa = 11.84 EE572 pKa = 4.18 TRR574 pKa = 11.84 GYY576 pKa = 9.74 LARR579 pKa = 11.84 SVASFVSGNWTNQNPLDD596 pKa = 4.22 PADD599 pKa = 4.17 ALRR602 pKa = 11.84 TAIVSTRR609 pKa = 11.84 ALINRR614 pKa = 11.84 SGRR617 pKa = 11.84 QDD619 pKa = 3.3 YY620 pKa = 11.13 ARR622 pKa = 11.84 LVGPALRR629 pKa = 11.84 LSHH632 pKa = 7.16 PLGTRR637 pKa = 11.84 NIIRR641 pKa = 11.84 LLSGDD646 pKa = 3.56 VALDD650 pKa = 3.9 DD651 pKa = 4.42 GPVYY655 pKa = 10.26 NTDD658 pKa = 2.56 GRR660 pKa = 11.84 IVHH663 pKa = 6.23 FRR665 pKa = 11.84 VEE667 pKa = 4.21 GQIEE671 pKa = 4.11 DD672 pKa = 3.92 ALPVSEE678 pKa = 4.82 RR679 pKa = 11.84 WPHH682 pKa = 5.68 NATDD686 pKa = 4.35 AYY688 pKa = 10.53 LLSHH692 pKa = 6.09 VSEE695 pKa = 4.59 VEE697 pKa = 3.97 RR698 pKa = 11.84 AALAATGTDD707 pKa = 3.18 AAALMLASSFSKK719 pKa = 10.24 GHH721 pKa = 6.2 SSLGSARR728 pKa = 11.84 RR729 pKa = 11.84 QVVRR733 pKa = 11.84 VAKK736 pKa = 10.54 RR737 pKa = 11.84 EE738 pKa = 3.91 TTRR741 pKa = 11.84 AHH743 pKa = 6.41 GFADD747 pKa = 4.47 ASDD750 pKa = 3.9 LLHH753 pKa = 7.48 RR754 pKa = 11.84 DD755 pKa = 3.17 RR756 pKa = 11.84 DD757 pKa = 3.85 KK758 pKa = 11.87 VGVLKK763 pKa = 10.65 AYY765 pKa = 9.85 PLVNLVKK772 pKa = 10.53 NRR774 pKa = 11.84 LRR776 pKa = 11.84 KK777 pKa = 8.33 EE778 pKa = 4.36 TISEE782 pKa = 4.03 LLILAGHH789 pKa = 6.23 TPGADD794 pKa = 3.64 PYY796 pKa = 9.11 RR797 pKa = 11.84 QAFGEE802 pKa = 4.21 HH803 pKa = 7.0 PEE805 pKa = 4.15 SKK807 pKa = 10.7 NIIGSLSHH815 pKa = 6.91 SDD817 pKa = 3.09 ASALGKK823 pKa = 9.07 ATRR826 pKa = 11.84 SGNIYY831 pKa = 8.43 TLTPVFVV838 pKa = 3.75
Molecular weight: 92.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.652
IPC2_protein 8.507
IPC_protein 8.448
Toseland 8.829
ProMoST 8.916
Dawson 9.268
Bjellqvist 9.238
Wikipedia 9.428
Rodwell 9.355
Grimsley 9.253
Solomon 9.326
Lehninger 9.282
Nozaki 9.341
DTASelect 9.107
Thurlkill 9.18
EMBOSS 9.385
Sillero 9.37
Patrickios 4.787
IPC_peptide 9.311
IPC2_peptide 8.17
IPC2.peptide.svr19 7.751
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1671
833
838
835.5
90.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.55 ± 0.432
1.197 ± 0.088
5.386 ± 0.259
4.249 ± 0.235
3.83 ± 0.009
8.917 ± 0.878
3.291 ± 0.579
3.71 ± 0.008
1.915 ± 0.808
10.293 ± 0.88
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.855 ± 0.041
3.591 ± 0.082
7.121 ± 1.957
2.214 ± 0.321
8.259 ± 0.162
6.822 ± 0.797
5.685 ± 0.239
6.463 ± 0.015
1.257 ± 0.313
2.394 ± 0.085
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here