Sphaeropsis sapinea RNA virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Victorivirus

Average proteome isoelectric point is 7.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q9YXE7|Q9YXE7_9VIRU Coat protein OS=Sphaeropsis sapinea RNA virus 1 OX=73497 PE=4 SV=1
MM1 pKa = 7.65PPISTAQWYY10 pKa = 9.35HH11 pKa = 5.63SLLLKK16 pKa = 10.75SLFVNRR22 pKa = 11.84FRR24 pKa = 11.84QSSLFGGRR32 pKa = 11.84VPLIPNTTIDD42 pKa = 3.95GYY44 pKa = 9.62ITTHH48 pKa = 5.19QHH50 pKa = 4.68HH51 pKa = 6.07TTPNFPMEE59 pKa = 4.19HH60 pKa = 6.88ALRR63 pKa = 11.84NAFLAAVIASPRR75 pKa = 11.84GGTLLTAPTKK85 pKa = 10.3KK86 pKa = 10.44GKK88 pKa = 8.93EE89 pKa = 3.95KK90 pKa = 10.42KK91 pKa = 10.43APLATYY97 pKa = 9.71RR98 pKa = 11.84RR99 pKa = 11.84YY100 pKa = 9.78RR101 pKa = 11.84ANIRR105 pKa = 11.84TEE107 pKa = 3.77STIAGNVDD115 pKa = 2.82ARR117 pKa = 11.84VAGIQYY123 pKa = 9.82EE124 pKa = 3.76IGARR128 pKa = 11.84YY129 pKa = 9.07EE130 pKa = 4.09RR131 pKa = 11.84VGEE134 pKa = 4.02AFAPVPEE141 pKa = 4.13EE142 pKa = 3.66QVLFEE147 pKa = 4.08AAYY150 pKa = 9.16PSSAALAEE158 pKa = 4.36DD159 pKa = 3.91FVGFARR165 pKa = 11.84KK166 pKa = 9.52YY167 pKa = 9.7SNFSATFAHH176 pKa = 6.78SSLAGLVEE184 pKa = 4.46RR185 pKa = 11.84VARR188 pKa = 11.84TLGALTVFPSGTFDD202 pKa = 3.05QDD204 pKa = 3.55AIRR207 pKa = 11.84GGRR210 pKa = 11.84PLMIAALGTLDD221 pKa = 4.52GPVNSLAGSVFIPRR235 pKa = 11.84LVDD238 pKa = 3.7SVISPDD244 pKa = 3.37VFTILINAAAGEE256 pKa = 4.37GSRR259 pKa = 11.84VITDD263 pKa = 3.5VLEE266 pKa = 4.52LDD268 pKa = 3.27ATTRR272 pKa = 11.84RR273 pKa = 11.84PIVPTLRR280 pKa = 11.84DD281 pKa = 3.22SSMLLPCVEE290 pKa = 4.4ALRR293 pKa = 11.84ILGANMAACDD303 pKa = 3.93QGPLFAFALVRR314 pKa = 11.84GLNAVLSLVGHH325 pKa = 5.75TDD327 pKa = 3.14EE328 pKa = 6.4AGVTRR333 pKa = 11.84DD334 pKa = 2.94IFRR337 pKa = 11.84VSGFDD342 pKa = 3.09IPFGGIHH349 pKa = 6.86FGLEE353 pKa = 4.17PYY355 pKa = 10.67AGLPAVASNAGADD368 pKa = 3.41ACCYY372 pKa = 10.64VDD374 pKa = 4.53ALLMTSGALVAHH386 pKa = 7.19CDD388 pKa = 3.33PGQEE392 pKa = 4.04YY393 pKa = 10.38GGRR396 pKa = 11.84WFPTVLQGTGPDD408 pKa = 3.53TAEE411 pKa = 3.82VRR413 pKa = 11.84PGQSQEE419 pKa = 3.79GTADD423 pKa = 3.12MANRR427 pKa = 11.84NRR429 pKa = 11.84GLLASSMPRR438 pKa = 11.84FAEE441 pKa = 4.62HH442 pKa = 6.27YY443 pKa = 10.31ARR445 pKa = 11.84GLGRR449 pKa = 11.84LFAIEE454 pKa = 3.69GDD456 pKa = 3.28ARR458 pKa = 11.84IVVNILSASARR469 pKa = 11.84LLPDD473 pKa = 2.89NCRR476 pKa = 11.84HH477 pKa = 5.59LRR479 pKa = 11.84YY480 pKa = 9.33PSVSPYY486 pKa = 10.22FWVEE490 pKa = 3.85PTSLLPPDD498 pKa = 4.44FLGTAAEE505 pKa = 4.26LNGCGSLAMRR515 pKa = 11.84GTSRR519 pKa = 11.84TRR521 pKa = 11.84QAWEE525 pKa = 4.36DD526 pKa = 3.34IEE528 pKa = 5.0RR529 pKa = 11.84VGDD532 pKa = 3.58EE533 pKa = 4.47DD534 pKa = 4.53VTFTGYY540 pKa = 10.15NVAMPFARR548 pKa = 11.84SSWFLIHH555 pKa = 6.88WLNHH559 pKa = 5.39PANGLGALRR568 pKa = 11.84VRR570 pKa = 11.84QLDD573 pKa = 3.84PNGVIHH579 pKa = 7.1PGPCADD585 pKa = 4.09NPDD588 pKa = 3.12VRR590 pKa = 11.84DD591 pKa = 3.44RR592 pKa = 11.84VEE594 pKa = 4.02ASLPITDD601 pKa = 4.11YY602 pKa = 11.27LWVRR606 pKa = 11.84GQSPFPAPGEE616 pKa = 3.96FLNLTGTLGLLARR629 pKa = 11.84HH630 pKa = 5.93LTMDD634 pKa = 4.71DD635 pKa = 3.69DD636 pKa = 5.37GIPTEE641 pKa = 4.03EE642 pKa = 4.26HH643 pKa = 6.72LPTGRR648 pKa = 11.84EE649 pKa = 3.78FRR651 pKa = 11.84DD652 pKa = 3.37TAVTVVAGRR661 pKa = 11.84PVGLPNGAHH670 pKa = 6.39NAYY673 pKa = 10.26DD674 pKa = 3.66SQARR678 pKa = 11.84RR679 pKa = 11.84ARR681 pKa = 11.84TRR683 pKa = 11.84ATRR686 pKa = 11.84EE687 pKa = 3.73LAASAARR694 pKa = 11.84ARR696 pKa = 11.84LYY698 pKa = 11.07GRR700 pKa = 11.84ATVAEE705 pKa = 4.27MPILTSAPVLRR716 pKa = 11.84AAPPRR721 pKa = 11.84PEE723 pKa = 4.19AVNPMEE729 pKa = 4.98GGGPGHH735 pKa = 7.34DD736 pKa = 4.0LARR739 pKa = 11.84HH740 pKa = 5.42SGMGRR745 pKa = 11.84HH746 pKa = 5.96TYY748 pKa = 10.23PPDD751 pKa = 3.53RR752 pKa = 11.84EE753 pKa = 4.28QRR755 pKa = 11.84GDD757 pKa = 3.43PAVPVPQHH765 pKa = 4.83QALRR769 pKa = 11.84APQMPRR775 pKa = 11.84PGPNPQGGGVIPPPPPLPPSGGGDD799 pKa = 3.33GSGPSSGGSCAQEE812 pKa = 4.32DD813 pKa = 4.8TNPAPPVAPGPAQPDD828 pKa = 4.06GPANEE833 pKa = 4.18

Molecular weight:
88.67 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q9YXE7|Q9YXE7_9VIRU Coat protein OS=Sphaeropsis sapinea RNA virus 1 OX=73497 PE=4 SV=1
MM1 pKa = 7.45NKK3 pKa = 10.13FIDD6 pKa = 3.68RR7 pKa = 11.84AEE9 pKa = 4.01QFGPVGDD16 pKa = 4.13YY17 pKa = 10.88LLSVVEE23 pKa = 4.5RR24 pKa = 11.84FPTVHH29 pKa = 6.84SYY31 pKa = 11.91GEE33 pKa = 4.07LSFVDD38 pKa = 3.17ALTRR42 pKa = 11.84LRR44 pKa = 11.84AMSNNLHH51 pKa = 6.81ANDD54 pKa = 4.67ALLPAAVSLLCLPFPLQVEE73 pKa = 4.51MSRR76 pKa = 11.84ADD78 pKa = 3.55VYY80 pKa = 11.78ALLVKK85 pKa = 9.59GTSLPFNFVRR95 pKa = 11.84STAHH99 pKa = 6.63LHH101 pKa = 6.01PLPDD105 pKa = 3.81DD106 pKa = 3.65VGRR109 pKa = 11.84PGSTRR114 pKa = 11.84YY115 pKa = 9.69SGRR118 pKa = 11.84FFARR122 pKa = 11.84LLNDD126 pKa = 2.99HH127 pKa = 6.29SFRR130 pKa = 11.84RR131 pKa = 11.84SCFPTKK137 pKa = 10.58KK138 pKa = 9.53MLPAEE143 pKa = 4.35VKK145 pKa = 10.36PNLALAPLIRR155 pKa = 11.84SFSTCAGPTLAGHH168 pKa = 6.73FLRR171 pKa = 11.84HH172 pKa = 5.67LAGHH176 pKa = 4.86VTEE179 pKa = 5.02DD180 pKa = 2.97VAMAAMVYY188 pKa = 10.54AHH190 pKa = 7.0GLVACYY196 pKa = 9.34GQKK199 pKa = 10.76GYY201 pKa = 10.72DD202 pKa = 3.01IAAWLVLRR210 pKa = 11.84PAAAKK215 pKa = 10.45GISTAIKK222 pKa = 9.99ALGANAHH229 pKa = 6.67DD230 pKa = 5.18FGAVLCEE237 pKa = 3.71AQTLLGRR244 pKa = 11.84AVSTIDD250 pKa = 3.43LAHH253 pKa = 6.03EE254 pKa = 4.08AEE256 pKa = 4.52YY257 pKa = 10.9RR258 pKa = 11.84CDD260 pKa = 3.74PDD262 pKa = 5.2LVAKK266 pKa = 9.51QVIEE270 pKa = 4.23PGEE273 pKa = 4.04EE274 pKa = 3.88LRR276 pKa = 11.84SHH278 pKa = 6.03IRR280 pKa = 11.84AVLAMEE286 pKa = 4.5LAGRR290 pKa = 11.84DD291 pKa = 3.62LSLPDD296 pKa = 4.28LDD298 pKa = 4.68SWWSSRR304 pKa = 11.84WLWCVNGSQNDD315 pKa = 3.4ASSRR319 pKa = 11.84LLGIDD324 pKa = 3.01TARR327 pKa = 11.84FRR329 pKa = 11.84EE330 pKa = 4.13FHH332 pKa = 5.07TRR334 pKa = 11.84EE335 pKa = 3.9YY336 pKa = 11.11RR337 pKa = 11.84RR338 pKa = 11.84MASEE342 pKa = 4.4ALTHH346 pKa = 6.23EE347 pKa = 5.76PITSWDD353 pKa = 3.49GYY355 pKa = 7.88TNISASPKK363 pKa = 9.75LEE365 pKa = 4.18HH366 pKa = 6.54GKK368 pKa = 7.98TRR370 pKa = 11.84AIFACDD376 pKa = 3.1TRR378 pKa = 11.84SYY380 pKa = 11.07FAFEE384 pKa = 4.1WLLGATQKK392 pKa = 10.68AWRR395 pKa = 11.84NHH397 pKa = 5.39RR398 pKa = 11.84VLLDD402 pKa = 3.81PGGGGHH408 pKa = 7.34LGISRR413 pKa = 11.84RR414 pKa = 11.84VRR416 pKa = 11.84SFMKK420 pKa = 10.36HH421 pKa = 5.01GGVNLMLDD429 pKa = 3.53FDD431 pKa = 5.41DD432 pKa = 5.75FNSHH436 pKa = 6.89HH437 pKa = 6.62SLNSQRR443 pKa = 11.84MLFGEE448 pKa = 4.29LCDD451 pKa = 3.95RR452 pKa = 11.84ANAPGWYY459 pKa = 9.23RR460 pKa = 11.84KK461 pKa = 9.7VLSDD465 pKa = 3.05SWGKK469 pKa = 7.92MHH471 pKa = 7.04VNIAGHH477 pKa = 4.98MRR479 pKa = 11.84PWLGTLPSGHH489 pKa = 7.16RR490 pKa = 11.84GTTIVNSVLNAAYY503 pKa = 9.67IRR505 pKa = 11.84MALGGPAFDD514 pKa = 5.23KK515 pKa = 10.34LTSLHH520 pKa = 6.47TGDD523 pKa = 4.85DD524 pKa = 3.79VYY526 pKa = 11.3IRR528 pKa = 11.84ADD530 pKa = 3.4TLTSCEE536 pKa = 4.21WILDD540 pKa = 3.61RR541 pKa = 11.84VRR543 pKa = 11.84SVGCRR548 pKa = 11.84INPAKK553 pKa = 10.39QSVGFGTGEE562 pKa = 4.26FLRR565 pKa = 11.84MAITQRR571 pKa = 11.84EE572 pKa = 4.18TRR574 pKa = 11.84GYY576 pKa = 9.74LARR579 pKa = 11.84SVASFVSGNWTNQNPLDD596 pKa = 4.22PADD599 pKa = 4.17ALRR602 pKa = 11.84TAIVSTRR609 pKa = 11.84ALINRR614 pKa = 11.84SGRR617 pKa = 11.84QDD619 pKa = 3.3YY620 pKa = 11.13ARR622 pKa = 11.84LVGPALRR629 pKa = 11.84LSHH632 pKa = 7.16PLGTRR637 pKa = 11.84NIIRR641 pKa = 11.84LLSGDD646 pKa = 3.56VALDD650 pKa = 3.9DD651 pKa = 4.42GPVYY655 pKa = 10.26NTDD658 pKa = 2.56GRR660 pKa = 11.84IVHH663 pKa = 6.23FRR665 pKa = 11.84VEE667 pKa = 4.21GQIEE671 pKa = 4.11DD672 pKa = 3.92ALPVSEE678 pKa = 4.82RR679 pKa = 11.84WPHH682 pKa = 5.68NATDD686 pKa = 4.35AYY688 pKa = 10.53LLSHH692 pKa = 6.09VSEE695 pKa = 4.59VEE697 pKa = 3.97RR698 pKa = 11.84AALAATGTDD707 pKa = 3.18AAALMLASSFSKK719 pKa = 10.24GHH721 pKa = 6.2SSLGSARR728 pKa = 11.84RR729 pKa = 11.84QVVRR733 pKa = 11.84VAKK736 pKa = 10.54RR737 pKa = 11.84EE738 pKa = 3.91TTRR741 pKa = 11.84AHH743 pKa = 6.41GFADD747 pKa = 4.47ASDD750 pKa = 3.9LLHH753 pKa = 7.48RR754 pKa = 11.84DD755 pKa = 3.17RR756 pKa = 11.84DD757 pKa = 3.85KK758 pKa = 11.87VGVLKK763 pKa = 10.65AYY765 pKa = 9.85PLVNLVKK772 pKa = 10.53NRR774 pKa = 11.84LRR776 pKa = 11.84KK777 pKa = 8.33EE778 pKa = 4.36TISEE782 pKa = 4.03LLILAGHH789 pKa = 6.23TPGADD794 pKa = 3.64PYY796 pKa = 9.11RR797 pKa = 11.84QAFGEE802 pKa = 4.21HH803 pKa = 7.0PEE805 pKa = 4.15SKK807 pKa = 10.7NIIGSLSHH815 pKa = 6.91SDD817 pKa = 3.09ASALGKK823 pKa = 9.07ATRR826 pKa = 11.84SGNIYY831 pKa = 8.43TLTPVFVV838 pKa = 3.75

Molecular weight:
92.16 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1671

833

838

835.5

90.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.55 ± 0.432

1.197 ± 0.088

5.386 ± 0.259

4.249 ± 0.235

3.83 ± 0.009

8.917 ± 0.878

3.291 ± 0.579

3.71 ± 0.008

1.915 ± 0.808

10.293 ± 0.88

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.855 ± 0.041

3.591 ± 0.082

7.121 ± 1.957

2.214 ± 0.321

8.259 ± 0.162

6.822 ± 0.797

5.685 ± 0.239

6.463 ± 0.015

1.257 ± 0.313

2.394 ± 0.085

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski