Aquamicrobium aerolatum DSM 21857

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Aquamicrobium; Aquamicrobium aerolatum

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3495 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3T2P5|A0A1I3T2P5_9RHIZ Sterol carrier protein OS=Aquamicrobium aerolatum DSM 21857 OX=1121003 GN=SAMN03080618_03537 PE=4 SV=1
MM1 pKa = 7.73ALTHH5 pKa = 6.75LSFDD9 pKa = 2.85ATYY12 pKa = 11.36YY13 pKa = 7.12MTEE16 pKa = 4.17RR17 pKa = 11.84PDD19 pKa = 3.35VLTAYY24 pKa = 10.19VNAGAEE30 pKa = 4.44TGTGMNWAQFAEE42 pKa = 3.84QHH44 pKa = 6.1YY45 pKa = 11.26NDD47 pKa = 4.76FGWKK51 pKa = 9.44EE52 pKa = 4.17GYY54 pKa = 9.71NPNAIFDD61 pKa = 3.85TSEE64 pKa = 3.73YY65 pKa = 10.24LAANIDD71 pKa = 3.8VLNAGVNPFQHH82 pKa = 6.19YY83 pKa = 9.81LQFGAYY89 pKa = 7.3EE90 pKa = 3.98KK91 pKa = 10.38RR92 pKa = 11.84APSDD96 pKa = 3.41SFISFEE102 pKa = 4.12DD103 pKa = 4.24FDD105 pKa = 3.87WEE107 pKa = 4.66TYY109 pKa = 10.55LGANSDD115 pKa = 3.73LTDD118 pKa = 3.72AGIEE122 pKa = 4.18TAEE125 pKa = 4.36DD126 pKa = 4.08AYY128 pKa = 10.86GHH130 pKa = 5.91YY131 pKa = 10.95VLFGQFEE138 pKa = 4.36VRR140 pKa = 11.84DD141 pKa = 4.23GKK143 pKa = 10.08PEE145 pKa = 3.74EE146 pKa = 5.2AIPSVPGEE154 pKa = 4.48TYY156 pKa = 10.57TLTTGVDD163 pKa = 3.05AGADD167 pKa = 3.65FTGTADD173 pKa = 3.38NDD175 pKa = 3.74TFRR178 pKa = 11.84AFDD181 pKa = 3.47MDD183 pKa = 4.39GPSGTAGATLQSWDD197 pKa = 4.15ILDD200 pKa = 4.14GGAGVDD206 pKa = 3.7TLNIATGAAADD217 pKa = 3.88NAAPTLRR224 pKa = 11.84NIEE227 pKa = 4.47IINNAHH233 pKa = 7.26LGQTINLASATGVQQIWTDD252 pKa = 3.25MTGFTGTAATRR263 pKa = 11.84YY264 pKa = 9.49NDD266 pKa = 3.4ASVATIFGIKK276 pKa = 9.4GAEE279 pKa = 4.12GSNSDD284 pKa = 4.09VNITFADD291 pKa = 3.77SLEE294 pKa = 4.25GDD296 pKa = 3.75TTVNFALEE304 pKa = 4.02GNAAGSYY311 pKa = 10.42AGFYY315 pKa = 10.98LEE317 pKa = 6.11DD318 pKa = 3.69EE319 pKa = 4.5GVEE322 pKa = 4.03NAVITVAEE330 pKa = 4.47GNGGFTTVSGVSSVTASGAGDD351 pKa = 3.43FGFYY355 pKa = 9.43TWDD358 pKa = 3.22NTTIEE363 pKa = 4.61SFNASAVTGDD373 pKa = 3.47VYY375 pKa = 10.98LTGAAGGTAAAFDD388 pKa = 4.07EE389 pKa = 4.83DD390 pKa = 4.84ANISSGAGNDD400 pKa = 3.47RR401 pKa = 11.84IYY403 pKa = 11.28VGNSDD408 pKa = 3.81GALTISTGAGNDD420 pKa = 3.63IIVSGSGNDD429 pKa = 3.77TIIAGAGKK437 pKa = 10.07NDD439 pKa = 3.52LTGGLGEE446 pKa = 4.53DD447 pKa = 4.01TFVLDD452 pKa = 4.48IKK454 pKa = 10.67GTVLGSLDD462 pKa = 4.05VINDD466 pKa = 3.42FNFGDD471 pKa = 3.96AQDD474 pKa = 3.68TLVFGEE480 pKa = 4.65TEE482 pKa = 3.91LTNDD486 pKa = 2.99NFASVGIAVSYY497 pKa = 11.26NDD499 pKa = 4.68LRR501 pKa = 11.84EE502 pKa = 4.05LAQGAFSDD510 pKa = 4.36DD511 pKa = 3.41VSFVAGTFGADD522 pKa = 2.51TYY524 pKa = 11.4VFHH527 pKa = 7.76DD528 pKa = 3.94ADD530 pKa = 4.14ADD532 pKa = 4.13GVADD536 pKa = 4.41AAVKK540 pKa = 10.7LIGTGSLLGADD551 pKa = 4.1AFEE554 pKa = 4.47VAAVV558 pKa = 3.41

Molecular weight: 57.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3J2R1|A0A1I3J2R1_9RHIZ Purine-binding chemotaxis protein CheW OS=Aquamicrobium aerolatum DSM 21857 OX=1121003 GN=SAMN03080618_00763 PE=4 SV=1
MM1 pKa = 7.31FCSTMRR7 pKa = 11.84SISNNHH13 pKa = 4.8VRR15 pKa = 11.84SEE17 pKa = 3.69ADD19 pKa = 3.08IIVVRR24 pKa = 11.84QRR26 pKa = 11.84PGHH29 pKa = 6.36PSQGLLIWGNTVFPCALGRR48 pKa = 11.84GGIKK52 pKa = 10.07ALKK55 pKa = 10.16RR56 pKa = 11.84EE57 pKa = 4.24GDD59 pKa = 3.46GATPLGSTRR68 pKa = 11.84LLYY71 pKa = 10.54GWFRR75 pKa = 11.84HH76 pKa = 5.29GKK78 pKa = 8.55IEE80 pKa = 4.32SRR82 pKa = 11.84RR83 pKa = 11.84SGLALRR89 pKa = 11.84QITPSDD95 pKa = 3.74GWCDD99 pKa = 3.36APADD103 pKa = 4.2RR104 pKa = 11.84NYY106 pKa = 10.59NRR108 pKa = 11.84PVTLPYY114 pKa = 9.5PASSEE119 pKa = 3.97TMEE122 pKa = 4.6RR123 pKa = 11.84KK124 pKa = 9.79DD125 pKa = 4.65RR126 pKa = 11.84LYY128 pKa = 10.92DD129 pKa = 3.26CCIVMDD135 pKa = 3.86YY136 pKa = 10.78NIRR139 pKa = 11.84PRR141 pKa = 11.84RR142 pKa = 11.84RR143 pKa = 11.84GRR145 pKa = 11.84GSAIFFHH152 pKa = 6.63IAKK155 pKa = 9.69PGFPPTQGCIAVEE168 pKa = 3.93PQVMQRR174 pKa = 11.84LLPHH178 pKa = 6.75LSPRR182 pKa = 11.84TRR184 pKa = 11.84IKK186 pKa = 10.59VVRR189 pKa = 3.95

Molecular weight: 21.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3495

0

3495

1083778

39

2826

310.1

33.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.05 ± 0.055

0.772 ± 0.011

5.758 ± 0.032

5.933 ± 0.041

3.855 ± 0.029

8.311 ± 0.04

2.054 ± 0.022

5.579 ± 0.034

3.325 ± 0.032

9.854 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.704 ± 0.022

2.795 ± 0.023

4.824 ± 0.027

3.244 ± 0.025

6.909 ± 0.041

5.632 ± 0.025

5.319 ± 0.031

7.531 ± 0.035

1.275 ± 0.018

2.275 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski