Amino acid dipepetide frequency for Aquamicrobium aerolatum DSM 21857

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.655AlaAla: 15.655 ± 0.184	0.973AlaCys: 0.973 ± 0.032	6.618AlaAsp: 6.618 ± 0.084	7.469AlaGlu: 7.469 ± 0.104	4.458AlaPhe: 4.458 ± 0.081	10.07AlaGly: 10.07 ± 0.114	2.275AlaHis: 2.275 ± 0.043	6.82AlaIle: 6.82 ± 0.1	3.84AlaLys: 3.84 ± 0.071	12.882AlaLeu: 12.882 ± 0.138	3.656AlaMet: 3.656 ± 0.063	3.015AlaAsn: 3.015 ± 0.06	5.072AlaPro: 5.072 ± 0.079	4.195AlaGln: 4.195 ± 0.074	8.325AlaArg: 8.325 ± 0.097	6.712AlaSer: 6.712 ± 0.08	6.045AlaThr: 6.045 ± 0.081	8.459AlaVal: 8.459 ± 0.087	1.328AlaTrp: 1.328 ± 0.037	2.633AlaTyr: 2.633 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.864CysAla: 0.864 ± 0.028	0.09CysCys: 0.09 ± 0.01	0.505CysAsp: 0.505 ± 0.022	0.414CysGlu: 0.414 ± 0.018	0.329CysPhe: 0.329 ± 0.018	0.811CysGly: 0.811 ± 0.031	0.209CysHis: 0.209 ± 0.014	0.421CysIle: 0.421 ± 0.022	0.161CysLys: 0.161 ± 0.011	0.686CysLeu: 0.686 ± 0.026	0.154CysMet: 0.154 ± 0.01	0.197CysAsn: 0.197 ± 0.011	0.408CysPro: 0.408 ± 0.022	0.227CysGln: 0.227 ± 0.013	0.555CysArg: 0.555 ± 0.026	0.441CysSer: 0.441 ± 0.022	0.354CysThr: 0.354 ± 0.019	0.608CysVal: 0.608 ± 0.025	0.105CysTrp: 0.105 ± 0.01	0.181CysTyr: 0.181 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	6.871AspAla: 6.871 ± 0.08	0.444AspCys: 0.444 ± 0.022	3.415AspAsp: 3.415 ± 0.075	3.889AspGlu: 3.889 ± 0.069	2.254AspPhe: 2.254 ± 0.044	5.097AspGly: 5.097 ± 0.082	1.308AspHis: 1.308 ± 0.04	3.235AspIle: 3.235 ± 0.06	1.815AspLys: 1.815 ± 0.046	5.761AspLeu: 5.761 ± 0.08	1.454AspMet: 1.454 ± 0.033	1.497AspAsn: 1.497 ± 0.04	3.111AspPro: 3.111 ± 0.054	1.709AspGln: 1.709 ± 0.04	4.135AspArg: 4.135 ± 0.067	2.177AspSer: 2.177 ± 0.043	2.696AspThr: 2.696 ± 0.058	4.288AspVal: 4.288 ± 0.062	0.891AspTrp: 0.891 ± 0.031	1.534AspTyr: 1.534 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	7.669GluAla: 7.669 ± 0.113	0.332GluCys: 0.332 ± 0.019	2.911GluAsp: 2.911 ± 0.051	3.463GluGlu: 3.463 ± 0.069	1.984GluPhe: 1.984 ± 0.043	4.332GluGly: 4.332 ± 0.061	1.233GluHis: 1.233 ± 0.035	3.934GluIle: 3.934 ± 0.065	2.464GluLys: 2.464 ± 0.051	5.554GluLeu: 5.554 ± 0.067	1.71GluMet: 1.71 ± 0.041	1.829GluAsn: 1.829 ± 0.041	2.751GluPro: 2.751 ± 0.059	2.297GluGln: 2.297 ± 0.055	5.155GluArg: 5.155 ± 0.079	2.407GluSer: 2.407 ± 0.045	3.622GluThr: 3.622 ± 0.062	3.923GluVal: 3.923 ± 0.063	0.722GluTrp: 0.722 ± 0.027	1.035GluTyr: 1.035 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	4.465PheAla: 4.465 ± 0.079	0.392PheCys: 0.392 ± 0.019	2.754PheAsp: 2.754 ± 0.057	2.326PheGlu: 2.326 ± 0.052	1.534PhePhe: 1.534 ± 0.048	3.834PheGly: 3.834 ± 0.074	0.796PheHis: 0.796 ± 0.026	1.945PheIle: 1.945 ± 0.047	1.004PheLys: 1.004 ± 0.033	3.533PheLeu: 3.533 ± 0.072	0.942PheMet: 0.942 ± 0.03	1.089PheAsn: 1.089 ± 0.031	1.606PhePro: 1.606 ± 0.033	1.06PheGln: 1.06 ± 0.029	2.341PheArg: 2.341 ± 0.052	2.43PheSer: 2.43 ± 0.056	1.964PheThr: 1.964 ± 0.045	2.978PheVal: 2.978 ± 0.063	0.585PheTrp: 0.585 ± 0.025	0.977PheTyr: 0.977 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	8.827GlyAla: 8.827 ± 0.112	0.737GlyCys: 0.737 ± 0.031	4.423GlyAsp: 4.423 ± 0.078	4.834GlyGlu: 4.834 ± 0.078	3.617GlyPhe: 3.617 ± 0.052	6.828GlyGly: 6.828 ± 0.12	1.832GlyHis: 1.832 ± 0.049	4.845GlyIle: 4.845 ± 0.076	3.385GlyLys: 3.385 ± 0.069	8.326GlyLeu: 8.326 ± 0.106	2.489GlyMet: 2.489 ± 0.053	2.388GlyAsn: 2.388 ± 0.055	3.086GlyPro: 3.086 ± 0.048	2.751GlyGln: 2.751 ± 0.055	5.685GlyArg: 5.685 ± 0.078	4.685GlySer: 4.685 ± 0.064	4.613GlyThr: 4.613 ± 0.074	6.232GlyVal: 6.232 ± 0.079	1.228GlyTrp: 1.228 ± 0.034	2.298GlyTyr: 2.298 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.351HisAla: 2.351 ± 0.054	0.215HisCys: 0.215 ± 0.014	1.271HisAsp: 1.271 ± 0.041	1.174HisGlu: 1.174 ± 0.031	0.848HisPhe: 0.848 ± 0.027	1.975HisGly: 1.975 ± 0.048	0.552HisHis: 0.552 ± 0.026	1.02HisIle: 1.02 ± 0.028	0.512HisLys: 0.512 ± 0.022	1.994HisLeu: 1.994 ± 0.042	0.526HisMet: 0.526 ± 0.02	0.507HisAsn: 0.507 ± 0.022	1.239HisPro: 1.239 ± 0.035	0.639HisGln: 0.639 ± 0.025	1.398HisArg: 1.398 ± 0.04	0.998HisSer: 0.998 ± 0.031	0.864HisThr: 0.864 ± 0.032	1.569HisVal: 1.569 ± 0.04	0.307HisTrp: 0.307 ± 0.016	0.584HisTyr: 0.584 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.719IleAla: 7.719 ± 0.107	0.557IleCys: 0.557 ± 0.025	3.831IleAsp: 3.831 ± 0.064	3.854IleGlu: 3.854 ± 0.078	1.942IlePhe: 1.942 ± 0.045	5.125IleGly: 5.125 ± 0.081	1.004IleHis: 1.004 ± 0.032	2.773IleIle: 2.773 ± 0.064	1.48IleLys: 1.48 ± 0.04	4.687IleLeu: 4.687 ± 0.084	1.19IleMet: 1.19 ± 0.033	1.651IleAsn: 1.651 ± 0.042	2.334IlePro: 2.334 ± 0.049	1.318IleGln: 1.318 ± 0.037	3.423IleArg: 3.423 ± 0.053	3.415IleSer: 3.415 ± 0.051	2.777IleThr: 2.777 ± 0.056	4.835IleVal: 4.835 ± 0.066	0.643IleTrp: 0.643 ± 0.024	1.23IleTyr: 1.23 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.237LysAla: 4.237 ± 0.069	0.153LysCys: 0.153 ± 0.013	1.701LysAsp: 1.701 ± 0.047	1.692LysGlu: 1.692 ± 0.044	0.925LysPhe: 0.925 ± 0.034	2.54LysGly: 2.54 ± 0.051	0.65LysHis: 0.65 ± 0.027	1.86LysIle: 1.86 ± 0.046	1.36LysLys: 1.36 ± 0.04	3.355LysLeu: 3.355 ± 0.058	0.884LysMet: 0.884 ± 0.03	0.944LysAsn: 0.944 ± 0.031	2.066LysPro: 2.066 ± 0.046	1.1LysGln: 1.1 ± 0.032	2.433LysArg: 2.433 ± 0.055	1.875LysSer: 1.875 ± 0.044	1.905LysThr: 1.905 ± 0.048	2.557LysVal: 2.557 ± 0.051	0.383LysTrp: 0.383 ± 0.018	0.629LysTyr: 0.629 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	12.748LeuAla: 12.748 ± 0.137	0.797LeuCys: 0.797 ± 0.029	5.891LeuAsp: 5.891 ± 0.076	5.544LeuGlu: 5.544 ± 0.078	3.702LeuPhe: 3.702 ± 0.076	7.896LeuGly: 7.896 ± 0.097	1.797LeuHis: 1.797 ± 0.05	5.199LeuIle: 5.199 ± 0.079	3.553LeuLys: 3.553 ± 0.061	9.13LeuLeu: 9.13 ± 0.137	2.489LeuMet: 2.489 ± 0.05	2.657LeuAsn: 2.657 ± 0.053	5.207LeuPro: 5.207 ± 0.075	2.788LeuGln: 2.788 ± 0.055	6.482LeuArg: 6.482 ± 0.085	6.619LeuSer: 6.619 ± 0.078	5.383LeuThr: 5.383 ± 0.077	7.547LeuVal: 7.547 ± 0.1	1.034LeuTrp: 1.034 ± 0.032	2.076LeuTyr: 2.076 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	3.315MetAla: 3.315 ± 0.054	0.171MetCys: 0.171 ± 0.013	1.208MetAsp: 1.208 ± 0.032	1.282MetGlu: 1.282 ± 0.036	0.837MetPhe: 0.837 ± 0.029	1.942MetGly: 1.942 ± 0.043	0.44MetHis: 0.44 ± 0.021	1.518MetIle: 1.518 ± 0.044	1.065MetLys: 1.065 ± 0.033	2.697MetLeu: 2.697 ± 0.055	0.83MetMet: 0.83 ± 0.027	0.918MetAsn: 0.918 ± 0.028	1.494MetPro: 1.494 ± 0.042	0.943MetGln: 0.943 ± 0.026	2.015MetArg: 2.015 ± 0.047	1.849MetSer: 1.849 ± 0.047	2.013MetThr: 2.013 ± 0.045	1.922MetVal: 1.922 ± 0.04	0.228MetTrp: 0.228 ± 0.015	0.356MetTyr: 0.356 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.37AsnAla: 3.37 ± 0.06	0.219AsnCys: 0.219 ± 0.014	1.564AsnAsp: 1.564 ± 0.04	1.427AsnGlu: 1.427 ± 0.034	1.059AsnPhe: 1.059 ± 0.035	2.554AsnGly: 2.554 ± 0.048	0.576AsnHis: 0.576 ± 0.023	1.549AsnIle: 1.549 ± 0.041	0.762AsnLys: 0.762 ± 0.026	2.656AsnLeu: 2.656 ± 0.051	0.703AsnMet: 0.703 ± 0.025	0.793AsnAsn: 0.793 ± 0.029	1.916AsnPro: 1.916 ± 0.041	0.843AsnGln: 0.843 ± 0.028	1.866AsnArg: 1.866 ± 0.043	1.414AsnSer: 1.414 ± 0.041	1.353AsnThr: 1.353 ± 0.039	2.159AsnVal: 2.159 ± 0.052	0.46AsnTrp: 0.46 ± 0.022	0.707AsnTyr: 0.707 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	5.643ProAla: 5.643 ± 0.097	0.269ProCys: 0.269 ± 0.016	3.359ProAsp: 3.359 ± 0.065	3.493ProGlu: 3.493 ± 0.058	2.03ProPhe: 2.03 ± 0.042	3.837ProGly: 3.837 ± 0.063	1.064ProHis: 1.064 ± 0.03	2.346ProIle: 2.346 ± 0.048	1.556ProLys: 1.556 ± 0.039	4.458ProLeu: 4.458 ± 0.064	1.166ProMet: 1.166 ± 0.032	1.345ProAsn: 1.345 ± 0.031	1.956ProPro: 1.956 ± 0.048	1.824ProGln: 1.824 ± 0.05	2.711ProArg: 2.711 ± 0.055	2.844ProSer: 2.844 ± 0.057	2.369ProThr: 2.369 ± 0.047	4.139ProVal: 4.139 ± 0.067	0.611ProTrp: 0.611 ± 0.027	1.216ProTyr: 1.216 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.996GlnAla: 3.996 ± 0.075	0.199GlnCys: 0.199 ± 0.014	1.558GlnAsp: 1.558 ± 0.04	1.723GlnGlu: 1.723 ± 0.04	1.219GlnPhe: 1.219 ± 0.035	2.277GlnGly: 2.277 ± 0.047	0.665GlnHis: 0.665 ± 0.027	2.099GlnIle: 2.099 ± 0.043	1.14GlnLys: 1.14 ± 0.033	3.091GlnLeu: 3.091 ± 0.058	1.043GlnMet: 1.043 ± 0.033	0.972GlnAsn: 0.972 ± 0.036	1.77GlnPro: 1.77 ± 0.044	1.327GlnGln: 1.327 ± 0.041	2.517GlnArg: 2.517 ± 0.051	1.859GlnSer: 1.859 ± 0.039	1.794GlnThr: 1.794 ± 0.041	2.226GlnVal: 2.226 ± 0.043	0.382GlnTrp: 0.382 ± 0.018	0.586GlnTyr: 0.586 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	7.559ArgAla: 7.559 ± 0.092	0.408ArgCys: 0.408 ± 0.018	4.173ArgAsp: 4.173 ± 0.062	4.308ArgGlu: 4.308 ± 0.067	2.939ArgPhe: 2.939 ± 0.055	4.604ArgGly: 4.604 ± 0.063	1.67ArgHis: 1.67 ± 0.037	4.083ArgIle: 4.083 ± 0.067	2.511ArgLys: 2.511 ± 0.049	7.349ArgLeu: 7.349 ± 0.098	1.972ArgMet: 1.972 ± 0.042	2.095ArgAsn: 2.095 ± 0.043	3.161ArgPro: 3.161 ± 0.058	2.633ArgGln: 2.633 ± 0.052	5.285ArgArg: 5.285 ± 0.092	3.791ArgSer: 3.791 ± 0.054	3.328ArgThr: 3.328 ± 0.055	4.561ArgVal: 4.561 ± 0.071	0.95ArgTrp: 0.95 ± 0.031	1.709ArgTyr: 1.709 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	6.323SerAla: 6.323 ± 0.075	0.432SerCys: 0.432 ± 0.024	3.182SerAsp: 3.182 ± 0.056	3.017SerGlu: 3.017 ± 0.055	2.393SerPhe: 2.393 ± 0.052	5.708SerGly: 5.708 ± 0.086	1.114SerHis: 1.114 ± 0.035	3.069SerIle: 3.069 ± 0.057	1.682SerLys: 1.682 ± 0.036	5.504SerLeu: 5.504 ± 0.076	1.53SerMet: 1.53 ± 0.041	1.521SerAsn: 1.521 ± 0.039	2.584SerPro: 2.584 ± 0.048	1.827SerGln: 1.827 ± 0.041	3.802SerArg: 3.802 ± 0.061	3.296SerSer: 3.296 ± 0.061	2.839SerThr: 2.839 ± 0.059	4.392SerVal: 4.392 ± 0.063	0.77SerTrp: 0.77 ± 0.031	1.331SerTyr: 1.331 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	5.998ThrAla: 5.998 ± 0.086	0.398ThrCys: 0.398 ± 0.021	2.786ThrAsp: 2.786 ± 0.065	2.655ThrGlu: 2.655 ± 0.054	2.04ThrPhe: 2.04 ± 0.046	5.031ThrGly: 5.031 ± 0.07	1.047ThrHis: 1.047 ± 0.028	3.232ThrIle: 3.232 ± 0.05	1.473ThrLys: 1.473 ± 0.042	5.687ThrLeu: 5.687 ± 0.076	1.375ThrMet: 1.375 ± 0.041	1.413ThrAsn: 1.413 ± 0.042	3.138ThrPro: 3.138 ± 0.053	1.574ThrGln: 1.574 ± 0.04	3.382ThrArg: 3.382 ± 0.052	2.963ThrSer: 2.963 ± 0.048	2.912ThrThr: 2.912 ± 0.059	4.277ThrVal: 4.277 ± 0.073	0.608ThrTrp: 0.608 ± 0.028	1.208ThrTyr: 1.208 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	9.28ValAla: 9.28 ± 0.108	0.588ValCys: 0.588 ± 0.026	4.267ValAsp: 4.267 ± 0.057	4.921ValGlu: 4.921 ± 0.076	2.913ValPhe: 2.913 ± 0.054	5.696ValGly: 5.696 ± 0.085	1.454ValHis: 1.454 ± 0.041	4.179ValIle: 4.179 ± 0.067	2.34ValLys: 2.34 ± 0.042	7.349ValLeu: 7.349 ± 0.086	2.081ValMet: 2.081 ± 0.047	2.044ValAsn: 2.044 ± 0.051	3.634ValPro: 3.634 ± 0.06	2.076ValGln: 2.076 ± 0.049	4.852ValArg: 4.852 ± 0.068	4.544ValSer: 4.544 ± 0.065	4.5ValThr: 4.5 ± 0.076	6.141ValVal: 6.141 ± 0.073	0.923ValTrp: 0.923 ± 0.032	1.534ValTyr: 1.534 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	1.2TrpAla: 1.2 ± 0.035	0.124TrpCys: 0.124 ± 0.011	0.585TrpAsp: 0.585 ± 0.023	0.532TrpGlu: 0.532 ± 0.022	0.547TrpPhe: 0.547 ± 0.026	0.802TrpGly: 0.802 ± 0.028	0.318TrpHis: 0.318 ± 0.018	0.646TrpIle: 0.646 ± 0.023	0.504TrpLys: 0.504 ± 0.021	1.582TrpLeu: 1.582 ± 0.04	0.366TrpMet: 0.366 ± 0.017	0.46TrpAsn: 0.46 ± 0.022	0.675TrpPro: 0.675 ± 0.027	0.555TrpGln: 0.555 ± 0.023	1.044TrpArg: 1.044 ± 0.033	0.776TrpSer: 0.776 ± 0.026	0.712TrpThr: 0.712 ± 0.026	0.791TrpVal: 0.791 ± 0.029	0.223TrpTrp: 0.223 ± 0.016	0.307TrpTyr: 0.307 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.409TyrAla: 2.409 ± 0.052	0.221TyrCys: 0.221 ± 0.015	1.579TyrAsp: 1.579 ± 0.043	1.308TyrGlu: 1.308 ± 0.034	0.984TyrPhe: 0.984 ± 0.031	2.152TyrGly: 2.152 ± 0.048	0.537TyrHis: 0.537 ± 0.019	1.016TyrIle: 1.016 ± 0.033	0.646TyrLys: 0.646 ± 0.027	2.259TyrLeu: 2.259 ± 0.045	0.483TyrMet: 0.483 ± 0.023	0.617TyrAsn: 0.617 ± 0.026	1.028TyrPro: 1.028 ± 0.034	0.758TyrGln: 0.758 ± 0.029	1.684TyrArg: 1.684 ± 0.041	1.221TyrSer: 1.221 ± 0.034	1.152TyrThr: 1.152 ± 0.032	1.712TyrVal: 1.712 ± 0.045	0.367TyrTrp: 0.367 ± 0.018	0.619TyrTyr: 0.619 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3495 proteins (1083778 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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