Sulfolobales archaeon SCGC AB-777_K20
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 579 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T9WQN8|A0A2T9WQN8_9CREN 50S ribosomal protein L40e OS=Sulfolobales archaeon SCGC AB-777_K20 OX=2005261 GN=rpl40e PE=3 SV=1
MM1 pKa = 7.37 VLLKK5 pKa = 10.92 CPVCGGDD12 pKa = 3.71 VQVDD16 pKa = 3.88 DD17 pKa = 4.12 NALPGEE23 pKa = 4.54 VIEE26 pKa = 4.89 HH27 pKa = 6.15 EE28 pKa = 4.41 CGALLEE34 pKa = 4.94 VYY36 pKa = 9.91 MKK38 pKa = 10.58 DD39 pKa = 2.69 GKK41 pKa = 10.65 LALRR45 pKa = 11.84 VAEE48 pKa = 4.76 QIGEE52 pKa = 4.19 DD53 pKa = 3.17 WGEE56 pKa = 3.7
Molecular weight: 6.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.14
IPC2_protein 4.19
IPC_protein 4.037
Toseland 3.872
ProMoST 4.101
Dawson 3.999
Bjellqvist 4.24
Wikipedia 3.91
Rodwell 3.872
Grimsley 3.783
Solomon 3.986
Lehninger 3.935
Nozaki 4.126
DTASelect 4.266
Thurlkill 3.91
EMBOSS 3.923
Sillero 4.151
Patrickios 3.121
IPC_peptide 3.986
IPC2_peptide 4.139
IPC2.peptide.svr19 4.075
Protein with the highest isoelectric point:
>tr|A0A2T9WR56|A0A2T9WR56_9CREN Zpr1 domain-containing protein OS=Sulfolobales archaeon SCGC AB-777_K20 OX=2005261 GN=DDW06_00375 PE=3 SV=1
MM1 pKa = 7.73 PRR3 pKa = 11.84 PALRR7 pKa = 11.84 SRR9 pKa = 11.84 SLARR13 pKa = 11.84 YY14 pKa = 7.55 YY15 pKa = 11.08 VRR17 pKa = 11.84 TPGGRR22 pKa = 11.84 TVIHH26 pKa = 6.05 YY27 pKa = 7.5 EE28 pKa = 3.76 RR29 pKa = 11.84 KK30 pKa = 9.29 RR31 pKa = 11.84 NGKK34 pKa = 7.87 ATCAVCGGEE43 pKa = 4.4 LRR45 pKa = 11.84 GVPTNQVYY53 pKa = 10.02 KK54 pKa = 10.51 YY55 pKa = 10.08 SKK57 pKa = 8.34 TEE59 pKa = 3.68 KK60 pKa = 10.37 RR61 pKa = 11.84 PEE63 pKa = 3.68 RR64 pKa = 11.84 PYY66 pKa = 11.4 AGTLCHH72 pKa = 6.79 RR73 pKa = 11.84 CLEE76 pKa = 4.16 KK77 pKa = 10.69 LIKK80 pKa = 9.84 QAVRR84 pKa = 11.84 SS85 pKa = 3.89
Molecular weight: 9.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.897
IPC_protein 10.672
Toseland 10.599
ProMoST 10.335
Dawson 10.745
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.979
Grimsley 10.804
Solomon 10.818
Lehninger 10.789
Nozaki 10.613
DTASelect 10.467
Thurlkill 10.613
EMBOSS 10.994
Sillero 10.672
Patrickios 10.701
IPC_peptide 10.833
IPC2_peptide 9.663
IPC2.peptide.svr19 8.31
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
579
0
579
150371
19
1461
259.7
29.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.944 ± 0.105
0.683 ± 0.038
4.886 ± 0.088
7.609 ± 0.11
3.967 ± 0.074
6.928 ± 0.083
1.315 ± 0.037
6.974 ± 0.102
7.092 ± 0.104
10.495 ± 0.132
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.143 ± 0.044
3.707 ± 0.086
4.188 ± 0.067
2.046 ± 0.05
5.735 ± 0.093
6.391 ± 0.083
4.71 ± 0.095
9.038 ± 0.089
0.898 ± 0.027
4.252 ± 0.08
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here