Aquimarina sp. TRL1
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4415 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7H8PPI0|A0A7H8PPI0_9FLAO Ribonuclease Z OS=Aquimarina sp. TRL1 OX=2736252 GN=rnz PE=3 SV=1
MM1 pKa = 7.73 KK2 pKa = 9.14 KK3 pKa = 6.75 TTYY6 pKa = 10.96 LLIVFLGLLYY16 pKa = 10.23 PKK18 pKa = 10.06 FVSAQCEE25 pKa = 4.17 VEE27 pKa = 4.35 EE28 pKa = 4.4 TFSVCDD34 pKa = 3.82 MEE36 pKa = 5.6 TVDD39 pKa = 5.0 HH40 pKa = 7.01 DD41 pKa = 4.54 TNGTPDD47 pKa = 4.37 GIINLFDD54 pKa = 3.39 EE55 pKa = 5.05 FQNVTGQSLTGGTWSIVPKK74 pKa = 10.32 YY75 pKa = 10.57 ASNLNPVTGEE85 pKa = 4.01 VNVWAFPNSTVTDD98 pKa = 3.66 TLEE101 pKa = 4.07 EE102 pKa = 4.39 YY103 pKa = 10.94 VFEE106 pKa = 5.21 FNTPACGDD114 pKa = 3.59 TPAVIFTMILGPFSGVALPLGANNANIEE142 pKa = 3.99 ICDD145 pKa = 3.88 TDD147 pKa = 3.85 TLNLFEE153 pKa = 5.69 ALTSNEE159 pKa = 4.79 SIPAPHH165 pKa = 7.24 LNGTWEE171 pKa = 4.72 FISNSSGAAFTGTFTLNDD189 pKa = 3.55 HH190 pKa = 6.48 SFSADD195 pKa = 2.79 IPYY198 pKa = 10.28 EE199 pKa = 3.81 ITIPPINRR207 pKa = 11.84 EE208 pKa = 3.89 IFEE211 pKa = 4.14 LRR213 pKa = 11.84 YY214 pKa = 9.42 VVPGMTPCATSEE226 pKa = 3.99 EE227 pKa = 4.4 TRR229 pKa = 11.84 VRR231 pKa = 11.84 VSVVRR236 pKa = 11.84 QVSAGIMEE244 pKa = 5.24 AGLICEE250 pKa = 4.27 GDD252 pKa = 3.65 ILAGLHH258 pKa = 6.77 DD259 pKa = 5.07 ADD261 pKa = 4.39 IDD263 pKa = 4.21 LRR265 pKa = 11.84 DD266 pKa = 3.77 DD267 pKa = 3.38 QFLRR271 pKa = 11.84 DD272 pKa = 3.81 EE273 pKa = 4.78 DD274 pKa = 4.51 VGGTWSSQGADD285 pKa = 3.48 PTNQISDD292 pKa = 3.84 EE293 pKa = 3.98 QDD295 pKa = 3.02 SVINLRR301 pKa = 11.84 EE302 pKa = 3.86 IYY304 pKa = 10.76 DD305 pKa = 3.64 NVINGGNNLRR315 pKa = 11.84 FGCVDD320 pKa = 3.98 FGFTYY325 pKa = 10.26 SVKK328 pKa = 10.16 KK329 pKa = 10.44 RR330 pKa = 11.84 SAVCEE335 pKa = 4.3 DD336 pKa = 3.87 DD337 pKa = 5.23 DD338 pKa = 3.93 EE339 pKa = 5.05 TVFFTFFEE347 pKa = 4.01 QLRR350 pKa = 11.84 PFNQAGEE357 pKa = 4.17 IPEE360 pKa = 4.04 VCANEE365 pKa = 4.2 EE366 pKa = 4.26 PGSIDD371 pKa = 5.36 LFDD374 pKa = 4.76 LLEE377 pKa = 4.08 FTNEE381 pKa = 3.53 NGIDD385 pKa = 4.22 FIYY388 pKa = 11.1 NDD390 pKa = 3.71 DD391 pKa = 4.89 AYY393 pKa = 11.26 VKK395 pKa = 9.45 WRR397 pKa = 11.84 MVSGPSDD404 pKa = 3.58 LPLSEE409 pKa = 4.32 DD410 pKa = 3.32 HH411 pKa = 6.92 KK412 pKa = 11.2 GTVQTLGAASGTYY425 pKa = 9.34 VFEE428 pKa = 4.83 FGVSPKK434 pKa = 9.94 INCPGGEE441 pKa = 4.1 AFCDD445 pKa = 3.57 PFAAEE450 pKa = 4.56 GEE452 pKa = 4.28 EE453 pKa = 4.5 GHH455 pKa = 7.0 CEE457 pKa = 4.11 FPCGVLTTQVTIRR470 pKa = 11.84 VLEE473 pKa = 3.85 FDD475 pKa = 4.07 YY476 pKa = 11.32 AGEE479 pKa = 4.25 DD480 pKa = 3.26 TDD482 pKa = 5.92 GINICISEE490 pKa = 4.08 NQIDD494 pKa = 4.43 LRR496 pKa = 11.84 SLLKK500 pKa = 10.35 IEE502 pKa = 4.59 GGNTIVDD509 pKa = 3.48 TGVWTNEE516 pKa = 3.57 AGEE519 pKa = 4.61 EE520 pKa = 3.87 IDD522 pKa = 3.49 NTFVFPEE529 pKa = 4.04 ISSPEE534 pKa = 3.91 AFRR537 pKa = 11.84 FTYY540 pKa = 9.55 TATSDD545 pKa = 3.47 MGCIDD550 pKa = 3.72 AATLEE555 pKa = 4.54 FTIHH559 pKa = 6.74 PLPVAGEE566 pKa = 4.42 DD567 pKa = 3.7 GTASVCSDD575 pKa = 3.42 NLTVTLFDD583 pKa = 4.19 HH584 pKa = 7.45 LEE586 pKa = 4.37 GNPDD590 pKa = 3.25 TTGIWTGPNGYY601 pKa = 8.05 TSTDD605 pKa = 2.81 HH606 pKa = 7.54 LGRR609 pKa = 11.84 FDD611 pKa = 4.82 QNDD614 pKa = 3.52 DD615 pKa = 3.62 TLPILGAGRR624 pKa = 11.84 FRR626 pKa = 11.84 YY627 pKa = 7.99 EE628 pKa = 3.76 VPGNAGCLDD637 pKa = 3.71 SDD639 pKa = 3.83 ASFVDD644 pKa = 4.35 VIIIEE649 pKa = 4.28 PEE651 pKa = 3.87 NIGNDD656 pKa = 3.44 RR657 pKa = 11.84 AEE659 pKa = 4.43 TFCKK663 pKa = 9.62 IDD665 pKa = 3.12 GRR667 pKa = 11.84 VNLFSLLDD675 pKa = 3.81 RR676 pKa = 11.84 NTVRR680 pKa = 11.84 TGTFEE685 pKa = 4.32 DD686 pKa = 4.08 TDD688 pKa = 3.63 DD689 pKa = 3.75 TGALNSEE696 pKa = 4.19 GVVEE700 pKa = 6.23 FEE702 pKa = 4.15 TLTNGIYY709 pKa = 9.99 NFRR712 pKa = 11.84 YY713 pKa = 9.65 IISNTAPCEE722 pKa = 3.96 EE723 pKa = 4.46 SSLNVAIQIVDD734 pKa = 3.79 LPEE737 pKa = 4.26 PQVGDD742 pKa = 3.52 QEE744 pKa = 4.92 FCILDD749 pKa = 3.51 AARR752 pKa = 11.84 LEE754 pKa = 4.87 DD755 pKa = 3.79 IVVDD759 pKa = 3.33 VLNYY763 pKa = 9.71 NWYY766 pKa = 6.93 EE767 pKa = 4.08 TLEE770 pKa = 4.09 GTIPIITNPILLDD783 pKa = 3.49 EE784 pKa = 4.25 QVFYY788 pKa = 10.74 IANVDD793 pKa = 3.63 ANNCEE798 pKa = 4.17 SEE800 pKa = 4.2 RR801 pKa = 11.84 VEE803 pKa = 4.16 VRR805 pKa = 11.84 INILNIGEE813 pKa = 4.29 RR814 pKa = 11.84 SAQGEE819 pKa = 4.73 LCTLDD824 pKa = 3.85 FQDD827 pKa = 5.29 GVSPNNDD834 pKa = 3.47 VINDD838 pKa = 3.82 TFDD841 pKa = 4.14 LVIEE845 pKa = 4.26 DD846 pKa = 4.4 VYY848 pKa = 11.16 NIPEE852 pKa = 4.5 AFPDD856 pKa = 3.64 FDD858 pKa = 4.72 LKK860 pKa = 10.86 IFNRR864 pKa = 11.84 YY865 pKa = 8.99 GSMVYY870 pKa = 10.02 EE871 pKa = 4.52 GNADD875 pKa = 3.55 TEE877 pKa = 4.38 EE878 pKa = 4.1 FRR880 pKa = 11.84 GEE882 pKa = 4.21 SNISLRR888 pKa = 11.84 LGDD891 pKa = 4.92 DD892 pKa = 4.15 LPSGTYY898 pKa = 9.9 FYY900 pKa = 10.74 IFTPNFEE907 pKa = 5.05 NNFPIQGSFYY917 pKa = 10.87 LSRR920 pKa = 5.17
Molecular weight: 101.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.592
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.884
DTASelect 4.101
Thurlkill 3.63
EMBOSS 3.694
Sillero 3.923
Patrickios 1.113
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A7H8PNX5|A0A7H8PNX5_9FLAO M20/M25/M40 family metallo-hydrolase OS=Aquimarina sp. TRL1 OX=2736252 GN=HN014_14800 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.15 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 KK13 pKa = 8.29 NKK15 pKa = 9.34 HH16 pKa = 4.03 GFRR19 pKa = 11.84 EE20 pKa = 4.2 RR21 pKa = 11.84 MASANGRR28 pKa = 11.84 KK29 pKa = 9.1 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.29 KK37 pKa = 10.31 GRR39 pKa = 11.84 KK40 pKa = 7.92 KK41 pKa = 10.69 LSVSSEE47 pKa = 3.9 LRR49 pKa = 11.84 HH50 pKa = 5.43 KK51 pKa = 10.29 HH52 pKa = 5.04
Molecular weight: 6.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.457
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.296
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.018
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.041
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4415
0
4415
1568201
15
9139
355.2
40.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.123 ± 0.042
0.791 ± 0.014
5.621 ± 0.122
6.605 ± 0.051
4.842 ± 0.047
6.452 ± 0.068
1.927 ± 0.025
8.208 ± 0.046
7.683 ± 0.089
8.952 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.984 ± 0.023
5.773 ± 0.044
3.425 ± 0.024
3.484 ± 0.027
3.534 ± 0.038
6.678 ± 0.034
6.524 ± 0.107
6.07 ± 0.038
1.044 ± 0.013
4.28 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here