Aerococcus viridans ATCC 11563 = CCUG 4311
Average proteome isoelectric point is 5.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1929 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D4YEF0|D4YEF0_9LACT Uncharacterized protein OS=Aerococcus viridans ATCC 11563 = CCUG 4311 OX=655812 GN=HMPREF0061_0239 PE=4 SV=1
MM1 pKa = 7.21 NKK3 pKa = 10.08 KK4 pKa = 10.48 LGILSLTALSLVLAACGNGGTTADD28 pKa = 3.77 NEE30 pKa = 4.13 EE31 pKa = 3.92 AAKK34 pKa = 10.31 SIKK37 pKa = 9.31 VTVNGEE43 pKa = 4.03 LASLDD48 pKa = 4.42 SISSTDD54 pKa = 3.41 SPSQNTIANVMEE66 pKa = 4.57 GLYY69 pKa = 10.35 RR70 pKa = 11.84 PADD73 pKa = 3.6 NGSNEE78 pKa = 4.2 LGVAAEE84 pKa = 4.41 EE85 pKa = 4.42 PTVSEE90 pKa = 5.32 DD91 pKa = 2.92 GLTYY95 pKa = 10.63 TFTIRR100 pKa = 11.84 DD101 pKa = 3.66 DD102 pKa = 4.67 ADD104 pKa = 3.23 WSNGDD109 pKa = 3.88 PVTAQDD115 pKa = 5.14 FVYY118 pKa = 9.49 TYY120 pKa = 10.47 QKK122 pKa = 9.56 TVTPEE127 pKa = 4.2 TIGGNANKK135 pKa = 10.14 FYY137 pKa = 10.21 MIEE140 pKa = 3.91 NAEE143 pKa = 4.35 AIANGEE149 pKa = 4.23 AEE151 pKa = 4.56 PDD153 pKa = 3.65 TLGVKK158 pKa = 10.5 AIDD161 pKa = 4.1 DD162 pKa = 3.73 KK163 pKa = 11.47 TLEE166 pKa = 4.17 FTLASPTTYY175 pKa = 8.96 FTDD178 pKa = 4.24 LLATPYY184 pKa = 10.14 YY185 pKa = 10.81 LPVNHH190 pKa = 6.3 NVATEE195 pKa = 4.17 LGSDD199 pKa = 3.53 YY200 pKa = 10.31 GTSAEE205 pKa = 3.96 NAVYY209 pKa = 10.46 NGPFEE214 pKa = 4.3 VSDD217 pKa = 3.65 WNSTEE222 pKa = 4.51 IEE224 pKa = 3.92 WTLTKK229 pKa = 10.52 NDD231 pKa = 4.44 EE232 pKa = 4.08 YY233 pKa = 10.95 WDD235 pKa = 4.07 ADD237 pKa = 4.07 NVDD240 pKa = 4.6 LDD242 pKa = 3.98 QIDD245 pKa = 3.37 WVMSKK250 pKa = 10.74 EE251 pKa = 3.77 NSTNVNLFEE260 pKa = 5.84 GGEE263 pKa = 3.98 LQYY266 pKa = 11.19 TEE268 pKa = 3.42 ITAPYY273 pKa = 8.27 VRR275 pKa = 11.84 EE276 pKa = 4.02 YY277 pKa = 11.12 QDD279 pKa = 2.97 TDD281 pKa = 3.54 VLHH284 pKa = 6.98 IEE286 pKa = 4.27 PKK288 pKa = 10.8 GMTGYY293 pKa = 9.46 MVFNTQSSPTNNVHH307 pKa = 6.14 FRR309 pKa = 11.84 RR310 pKa = 11.84 AISQAYY316 pKa = 9.53 DD317 pKa = 2.93 KK318 pKa = 10.83 EE319 pKa = 4.52 AFVDD323 pKa = 4.23 AVLDD327 pKa = 4.72 DD328 pKa = 4.58 GSQAAGGWIPSDD340 pKa = 3.58 FGSDD344 pKa = 3.61 PEE346 pKa = 4.22 SGIDD350 pKa = 3.61 FRR352 pKa = 11.84 EE353 pKa = 4.09 EE354 pKa = 3.68 NGDD357 pKa = 4.21 LMTFDD362 pKa = 5.35 LEE364 pKa = 4.3 AAQEE368 pKa = 4.03 EE369 pKa = 4.7 WALALEE375 pKa = 4.33 EE376 pKa = 5.16 LGTDD380 pKa = 3.35 TVEE383 pKa = 4.91 IEE385 pKa = 4.78 LLTSDD390 pKa = 4.06 TDD392 pKa = 3.56 TSKK395 pKa = 10.33 ATSEE399 pKa = 3.96 YY400 pKa = 10.83 LQAQLQTNLPGLTVTVRR417 pKa = 11.84 NVPLKK422 pKa = 10.49 SRR424 pKa = 11.84 QAITGTGEE432 pKa = 3.44 FDD434 pKa = 3.47 IVYY437 pKa = 8.4 GTYY440 pKa = 10.05 NPSYY444 pKa = 10.96 ADD446 pKa = 3.23 PTAFLDD452 pKa = 4.25 FFITGSPLSSGDD464 pKa = 3.8 FSDD467 pKa = 3.35 ATYY470 pKa = 11.07 DD471 pKa = 3.52 EE472 pKa = 5.47 LMTEE476 pKa = 4.62 GKK478 pKa = 7.96 ITNALDD484 pKa = 3.61 PAARR488 pKa = 11.84 WDD490 pKa = 3.68 NFLAAEE496 pKa = 4.54 KK497 pKa = 10.97 YY498 pKa = 10.32 LVEE501 pKa = 4.65 DD502 pKa = 4.11 AAAVSPIYY510 pKa = 10.39 QGAYY514 pKa = 10.47 ANLIDD519 pKa = 4.39 PALEE523 pKa = 3.97 NVINQPNGVAQYY535 pKa = 10.79 YY536 pKa = 9.5 RR537 pKa = 11.84 AATYY541 pKa = 10.9 NVTEE545 pKa = 4.17
Molecular weight: 59.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.757
IPC_protein 3.783
Toseland 3.567
ProMoST 3.91
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.656
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.706
Nozaki 3.859
DTASelect 4.075
Thurlkill 3.605
EMBOSS 3.681
Sillero 3.897
Patrickios 1.418
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.789
Protein with the highest isoelectric point:
>tr|D4YHD1|D4YHD1_9LACT Thioredoxin-like_fold domain-containing protein OS=Aerococcus viridans ATCC 11563 = CCUG 4311 OX=655812 GN=HMPREF0061_1270 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 9.2 KK9 pKa = 7.96 RR10 pKa = 11.84 HH11 pKa = 4.8 RR12 pKa = 11.84 QKK14 pKa = 10.16 VHH16 pKa = 5.49 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 10.16 NGRR28 pKa = 11.84 HH29 pKa = 4.13 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.53 GRR39 pKa = 11.84 KK40 pKa = 9.06 VISAA44 pKa = 4.05
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1929
0
1929
571387
37
3427
296.2
33.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.115 ± 0.067
0.436 ± 0.014
6.407 ± 0.042
7.141 ± 0.064
4.298 ± 0.043
6.733 ± 0.057
1.896 ± 0.028
7.594 ± 0.054
5.821 ± 0.052
9.096 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.725 ± 0.029
4.848 ± 0.041
3.394 ± 0.034
4.335 ± 0.052
3.947 ± 0.042
5.76 ± 0.056
5.746 ± 0.038
7.102 ± 0.045
0.847 ± 0.018
3.758 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here