Aquitalea magnusonii
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4103 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F3K7A7|A0A0F3K7A7_9NEIS Anticodon nuclease OS=Aquitalea magnusonii OX=332411 GN=VI06_17720 PE=4 SV=1
MM1 pKa = 7.23 NAVSYY6 pKa = 8.79 VIRR9 pKa = 11.84 DD10 pKa = 3.81 AVCAGIPCPADD21 pKa = 3.11 GCAGDD26 pKa = 5.7 KK27 pKa = 10.89 INLDD31 pKa = 3.91 TNSVNDD37 pKa = 4.3 PDD39 pKa = 3.88 PTFLVVAKK47 pKa = 10.16 SDD49 pKa = 4.18 AIHH52 pKa = 7.59 DD53 pKa = 3.9 IFAGVAVLIDD63 pKa = 3.44 VSCRR67 pKa = 11.84 DD68 pKa = 3.35 GG69 pKa = 3.63
Molecular weight: 7.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.935
IPC_protein 3.884
Toseland 3.643
ProMoST 4.113
Dawson 3.923
Bjellqvist 4.088
Wikipedia 3.961
Rodwell 3.719
Grimsley 3.567
Solomon 3.91
Lehninger 3.859
Nozaki 4.062
DTASelect 4.406
Thurlkill 3.745
EMBOSS 3.948
Sillero 4.024
Patrickios 1.964
IPC_peptide 3.897
IPC2_peptide 3.986
IPC2.peptide.svr19 3.919
Protein with the highest isoelectric point:
>tr|A0A0F3K457|A0A0F3K457_9NEIS BolA family transcriptional regulator OS=Aquitalea magnusonii OX=332411 GN=VI06_20745 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSTTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.24 RR14 pKa = 11.84 THH16 pKa = 5.92 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.7 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVIAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.8 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4103
0
4103
1282727
29
4629
312.6
34.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.489 ± 0.05
1.044 ± 0.013
5.201 ± 0.032
5.199 ± 0.036
3.576 ± 0.025
7.746 ± 0.034
2.429 ± 0.019
4.899 ± 0.027
3.555 ± 0.033
11.671 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.638 ± 0.021
2.981 ± 0.028
4.71 ± 0.026
5.013 ± 0.038
6.072 ± 0.033
5.955 ± 0.038
4.787 ± 0.035
7.078 ± 0.03
1.441 ± 0.018
2.515 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here