Nilaparvata lugens reovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Fijivirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q83863|Q83863_9REOV 95.1KD putative nonstructural protein OS=Nilaparvata lugens reovirus OX=33724 PE=4 SV=1
MM1 pKa = 7.61AFKK4 pKa = 10.51FDD6 pKa = 3.95ASNLNKK12 pKa = 9.24TRR14 pKa = 11.84QALATMSNPSFQPKK28 pKa = 9.85GFPSNSTQFNGSNGRR43 pKa = 11.84KK44 pKa = 9.62GKK46 pKa = 10.53FPDD49 pKa = 3.5AKK51 pKa = 9.62KK52 pKa = 7.26TTLSSPPLFNEE63 pKa = 3.8SQKK66 pKa = 10.77PKK68 pKa = 9.31TVSSMITSPIIGSDD82 pKa = 3.72RR83 pKa = 11.84IFTSSEE89 pKa = 3.91FIHH92 pKa = 5.8PQKK95 pKa = 10.74FVMTEE100 pKa = 3.78YY101 pKa = 9.69VQKK104 pKa = 10.86SIAKK108 pKa = 9.27FEE110 pKa = 4.27THH112 pKa = 6.7GSIDD116 pKa = 3.83PFAKK120 pKa = 10.52KK121 pKa = 9.88PDD123 pKa = 3.82FDD125 pKa = 4.62DD126 pKa = 4.19LCLILYY132 pKa = 7.74TPAASKK138 pKa = 11.08HH139 pKa = 5.08EE140 pKa = 4.18LSLANAQLVCSDD152 pKa = 3.26IDD154 pKa = 3.81SLNLHH159 pKa = 6.14SMHH162 pKa = 6.88VIDD165 pKa = 4.04GTKK168 pKa = 9.89LYY170 pKa = 11.48SMAANVQYY178 pKa = 11.25LNAGVIYY185 pKa = 10.07LPKK188 pKa = 9.96SHH190 pKa = 7.1WDD192 pKa = 3.02AFTSKK197 pKa = 7.69THH199 pKa = 5.79AHH201 pKa = 5.92VKK203 pKa = 9.76MEE205 pKa = 4.27MNFACSPVHH214 pKa = 6.54LNKK217 pKa = 10.14LRR219 pKa = 11.84LAHH222 pKa = 6.17WMSPFLLTKK231 pKa = 10.22MSVSFQLQVEE241 pKa = 4.64IYY243 pKa = 10.45DD244 pKa = 3.65HH245 pKa = 7.2FEE247 pKa = 3.86QFVKK251 pKa = 9.62NTHH254 pKa = 6.47ANVDD258 pKa = 3.17SPMRR262 pKa = 11.84VFGSKK267 pKa = 9.78NGKK270 pKa = 8.78EE271 pKa = 4.29CGSLEE276 pKa = 4.14PEE278 pKa = 4.34EE279 pKa = 5.75DD280 pKa = 3.68LDD282 pKa = 5.06SPLDD286 pKa = 3.71FSKK289 pKa = 11.59NNVCLIDD296 pKa = 3.82VNDD299 pKa = 4.7DD300 pKa = 3.26FTSAEE305 pKa = 3.8RR306 pKa = 11.84KK307 pKa = 7.41MHH309 pKa = 5.8VNLNVFRR316 pKa = 11.84NAITFVKK323 pKa = 10.59KK324 pKa = 10.51EE325 pKa = 4.07FGRR328 pKa = 11.84ANGGNSLVQKK338 pKa = 9.72MRR340 pKa = 11.84ASKK343 pKa = 10.9VFLLYY348 pKa = 10.44FDD350 pKa = 5.62LNNTSEE356 pKa = 4.11TRR358 pKa = 11.84NRR360 pKa = 11.84KK361 pKa = 8.49QRR363 pKa = 11.84DD364 pKa = 3.42PSVVNTDD371 pKa = 3.3TLKK374 pKa = 11.19AFDD377 pKa = 4.02GFNTVFVIVDD387 pKa = 3.95SEE389 pKa = 4.33GMIASSVSMSSVEE402 pKa = 4.08AMLMSLKK409 pKa = 10.22QVSLVDD415 pKa = 3.13YY416 pKa = 10.13HH417 pKa = 7.73LNYY420 pKa = 10.65CSDD423 pKa = 3.09VLMNEE428 pKa = 3.77GHH430 pKa = 6.55VYY432 pKa = 11.03SLMLSCICKK441 pKa = 8.67TLSFGGRR448 pKa = 11.84ILISSWIHH456 pKa = 5.89ALFEE460 pKa = 4.6GEE462 pKa = 4.8KK463 pKa = 9.21KK464 pKa = 8.67TATEE468 pKa = 3.97TFEE471 pKa = 4.07EE472 pKa = 4.4LKK474 pKa = 10.68KK475 pKa = 11.23LKK477 pKa = 10.45DD478 pKa = 3.83VEE480 pKa = 4.51DD481 pKa = 4.2KK482 pKa = 11.37KK483 pKa = 10.76PDD485 pKa = 3.05AMTARR490 pKa = 11.84KK491 pKa = 7.37PTTDD495 pKa = 3.13EE496 pKa = 4.56NEE498 pKa = 4.58DD499 pKa = 3.4EE500 pKa = 4.45NEE502 pKa = 4.0AAKK505 pKa = 9.26TAFDD509 pKa = 3.3KK510 pKa = 11.43HH511 pKa = 6.52LDD513 pKa = 3.55KK514 pKa = 11.47VEE516 pKa = 4.9KK517 pKa = 10.22ILSEE521 pKa = 4.29DD522 pKa = 4.24SEE524 pKa = 4.66NQDD527 pKa = 2.84GTQTDD532 pKa = 3.7TEE534 pKa = 4.18EE535 pKa = 5.24RR536 pKa = 11.84LTQTTAAPNPIEE548 pKa = 4.31TTFPAAQTQPDD559 pKa = 3.71QTQKK563 pKa = 9.77TEE565 pKa = 4.08STDD568 pKa = 3.57QNDD571 pKa = 3.89SNVQDD576 pKa = 3.72SQEE579 pKa = 4.06NQSKK583 pKa = 10.83DD584 pKa = 3.36NQTRR588 pKa = 11.84SHH590 pKa = 7.5PLPDD594 pKa = 3.52QDD596 pKa = 5.66SVDD599 pKa = 4.34DD600 pKa = 3.87STKK603 pKa = 10.77EE604 pKa = 3.79SDD606 pKa = 3.56ASSSATFDD614 pKa = 3.17SATEE618 pKa = 4.08FKK620 pKa = 10.32EE621 pKa = 3.84AANASVLVDD630 pKa = 3.92LQDD633 pKa = 3.32IFSNASSQTAQSSTTSSTKK652 pKa = 9.51STSDD656 pKa = 2.78QATFKK661 pKa = 10.32NTVSKK666 pKa = 9.32QTDD669 pKa = 3.24SRR671 pKa = 11.84SAFPDD676 pKa = 3.12IQEE679 pKa = 4.41KK680 pKa = 9.91QLPAPPNSLDD690 pKa = 3.89DD691 pKa = 5.16GISASMGLPPPKK703 pKa = 10.06IMTVDD708 pKa = 3.46EE709 pKa = 4.36VEE711 pKa = 4.08AALFGTKK718 pKa = 9.99EE719 pKa = 4.13NGSVASPVKK728 pKa = 10.31NVPIHH733 pKa = 6.27EE734 pKa = 4.37KK735 pKa = 10.68ASPKK739 pKa = 9.51NANGRR744 pKa = 11.84TLPPVPEE751 pKa = 4.18EE752 pKa = 4.06VPIGNVGGPRR762 pKa = 11.84QLKK765 pKa = 10.47VSFDD769 pKa = 3.94DD770 pKa = 6.41LMDD773 pKa = 4.44FSQNASAVQNSCPSNNIPPSPSEE796 pKa = 3.86SSEE799 pKa = 4.01TTKK802 pKa = 10.52QQSSSCGSSNNPSSSTGYY820 pKa = 10.79VGDD823 pKa = 3.8NTKK826 pKa = 9.6TEE828 pKa = 4.33PLPTTPSPSDD838 pKa = 3.21AASTKK843 pKa = 10.75SVDD846 pKa = 3.41NDD848 pKa = 3.7ALSTPPNGNVDD859 pKa = 3.45DD860 pKa = 4.96HH861 pKa = 5.72QAKK864 pKa = 10.41SNPSSVFDD872 pKa = 4.08FTASCQSSKK881 pKa = 11.02SDD883 pKa = 4.13SGLCTPTASTTSDD896 pKa = 3.23PNQALFYY903 pKa = 10.28QQFSFIKK910 pKa = 9.89EE911 pKa = 4.15YY912 pKa = 11.18NCTPEE917 pKa = 3.88NLRR920 pKa = 11.84LLLEE924 pKa = 4.23TVRR927 pKa = 11.84ARR929 pKa = 11.84LIPMHH934 pKa = 6.64LVHH937 pKa = 7.08PEE939 pKa = 3.77VVPNLDD945 pKa = 3.75SVCEE949 pKa = 3.96PRR951 pKa = 11.84NLSLAFGLSTLCYY964 pKa = 10.27DD965 pKa = 4.19PNPNTGCC972 pKa = 3.92

Molecular weight:
106.41 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q83865|Q83865_9REOV 33KD putative nonstructural protein OS=Nilaparvata lugens reovirus OX=33724 PE=4 SV=1
MM1 pKa = 7.36EE2 pKa = 5.3HH3 pKa = 7.26LEE5 pKa = 4.6TITDD9 pKa = 5.05LIFPIFINRR18 pKa = 11.84FRR20 pKa = 11.84QNKK23 pKa = 8.22YY24 pKa = 9.32NNRR27 pKa = 11.84SNTKK31 pKa = 9.89LLNKK35 pKa = 9.44PSPVDD40 pKa = 3.0IKK42 pKa = 10.71RR43 pKa = 11.84YY44 pKa = 7.49YY45 pKa = 10.47SSYY48 pKa = 9.95NSQHH52 pKa = 6.1AVLLRR57 pKa = 11.84VTYY60 pKa = 8.97MPHH63 pKa = 6.27HH64 pKa = 6.22EE65 pKa = 5.19RR66 pKa = 11.84YY67 pKa = 9.2LQSLNIQDD75 pKa = 3.65AQKK78 pKa = 10.68RR79 pKa = 11.84GYY81 pKa = 6.93TAKK84 pKa = 10.72NLDD87 pKa = 3.87LMWYY91 pKa = 10.02RR92 pKa = 11.84LFTGTQRR99 pKa = 11.84GIYY102 pKa = 8.89YY103 pKa = 9.25PPHH106 pKa = 5.87VFNNITKK113 pKa = 10.08KK114 pKa = 10.9YY115 pKa = 9.52GFGLIMNDD123 pKa = 2.59IQTKK127 pKa = 10.35VDD129 pKa = 4.01LEE131 pKa = 4.63TKK133 pKa = 7.49NTHH136 pKa = 5.15YY137 pKa = 11.07VVVDD141 pKa = 3.87FDD143 pKa = 3.88EE144 pKa = 6.74AEE146 pKa = 4.01EE147 pKa = 4.2SDD149 pKa = 5.04NGIQNPGTIIQFSGNGIPMKK169 pKa = 10.63YY170 pKa = 9.87KK171 pKa = 10.74SSTNKK176 pKa = 9.55QFLNNIIKK184 pKa = 10.56LEE186 pKa = 3.95RR187 pKa = 11.84KK188 pKa = 9.42ILDD191 pKa = 3.14EE192 pKa = 4.27SKK194 pKa = 10.8RR195 pKa = 11.84VDD197 pKa = 3.71PLALLNEE204 pKa = 5.04SIQTEE209 pKa = 4.33CAKK212 pKa = 11.04GNVVKK217 pKa = 10.41LQKK220 pKa = 10.49RR221 pKa = 11.84EE222 pKa = 4.34DD223 pKa = 3.11ISFAFEE229 pKa = 3.98CYY231 pKa = 9.67EE232 pKa = 3.8YY233 pKa = 10.55TGNYY237 pKa = 8.59VKK239 pKa = 10.82GNSFIKK245 pKa = 10.16MLLNVDD251 pKa = 4.53FTPIYY256 pKa = 7.93YY257 pKa = 9.88QYY259 pKa = 11.55YY260 pKa = 8.39LTDD263 pKa = 3.36TTNEE267 pKa = 3.78IEE269 pKa = 4.35SYY271 pKa = 9.37PVYY274 pKa = 10.38VQSVLNRR281 pKa = 11.84MKK283 pKa = 10.64LVRR286 pKa = 11.84VPKK289 pKa = 10.25IEE291 pKa = 3.86NEE293 pKa = 3.72IMNGVMPTEE302 pKa = 4.36IAISTCGIVNVRR314 pKa = 11.84FNGWVFTNLAPRR326 pKa = 11.84SKK328 pKa = 10.11FRR330 pKa = 11.84LYY332 pKa = 9.04GTEE335 pKa = 4.07KK336 pKa = 9.93IKK338 pKa = 10.85HH339 pKa = 6.17FIMNYY344 pKa = 8.09VSSFSEE350 pKa = 4.23YY351 pKa = 10.71QMVPYY356 pKa = 10.05FEE358 pKa = 6.23SGAEE362 pKa = 3.67YY363 pKa = 10.37TMRR366 pKa = 11.84IGRR369 pKa = 11.84PIATSFSTFSRR380 pKa = 11.84EE381 pKa = 3.59RR382 pKa = 11.84MAEE385 pKa = 3.87NFKK388 pKa = 10.99DD389 pKa = 3.79AVRR392 pKa = 11.84EE393 pKa = 3.85RR394 pKa = 11.84MPIWVVANKK403 pKa = 10.55GSGKK407 pKa = 8.23TVLRR411 pKa = 11.84KK412 pKa = 9.4EE413 pKa = 4.02LEE415 pKa = 4.19EE416 pKa = 4.41IGFNVIDD423 pKa = 3.68SDD425 pKa = 4.09AYY427 pKa = 8.96GWFVSKK433 pKa = 10.41VAYY436 pKa = 9.02IHH438 pKa = 7.27KK439 pKa = 9.88EE440 pKa = 3.97VNQNTLSTIEE450 pKa = 4.6LSQDD454 pKa = 3.29HH455 pKa = 6.91INRR458 pKa = 11.84LVSEE462 pKa = 4.28VLEE465 pKa = 4.07EE466 pKa = 4.3DD467 pKa = 3.7RR468 pKa = 11.84GISYY472 pKa = 10.13FNVIMYY478 pKa = 10.6SLTKK482 pKa = 9.8QKK484 pKa = 10.9RR485 pKa = 11.84DD486 pKa = 3.16INKK489 pKa = 8.8VLTFPYY495 pKa = 9.06VTHH498 pKa = 6.96RR499 pKa = 11.84WNDD502 pKa = 3.17TLTTFGKK509 pKa = 10.4VFTEE513 pKa = 4.29IADD516 pKa = 3.89SPAIGYY522 pKa = 8.21PRR524 pKa = 11.84FIEE527 pKa = 4.58GCMEE531 pKa = 3.89YY532 pKa = 10.72FRR534 pKa = 11.84VRR536 pKa = 11.84EE537 pKa = 4.08TLHH540 pKa = 6.76KK541 pKa = 10.17PIIFFAHH548 pKa = 6.11TEE550 pKa = 4.22SEE552 pKa = 4.1LSRR555 pKa = 11.84ISIRR559 pKa = 11.84HH560 pKa = 5.27FSCVIYY566 pKa = 10.86NSIDD570 pKa = 3.28SRR572 pKa = 11.84SILSKK577 pKa = 10.41RR578 pKa = 11.84ADD580 pKa = 3.39PVVEE584 pKa = 4.43LMLHH588 pKa = 6.62AYY590 pKa = 10.68YY591 pKa = 11.34YY592 pKa = 8.74MNQVAIDD599 pKa = 4.65KK600 pKa = 10.71IPFSLFRR607 pKa = 11.84QWLGMSNKK615 pKa = 9.26VVDD618 pKa = 4.54EE619 pKa = 4.42SVVAQLIVRR628 pKa = 11.84EE629 pKa = 4.23

Molecular weight:
73.48 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

8916

206

1442

810.5

92.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.957 ± 0.293

1.447 ± 0.196

6.18 ± 0.302

5.002 ± 0.366

5.316 ± 0.239

4.565 ± 0.25

2.131 ± 0.146

7.279 ± 0.511

6.292 ± 0.262

8.636 ± 0.408

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.838 ± 0.099

6.819 ± 0.239

3.892 ± 0.312

3.297 ± 0.174

4.307 ± 0.339

8.457 ± 0.523

6.886 ± 0.242

6.359 ± 0.251

0.729 ± 0.073

4.61 ± 0.415

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski