Nilaparvata lugens reovirus
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q83863|Q83863_9REOV 95.1KD putative nonstructural protein OS=Nilaparvata lugens reovirus OX=33724 PE=4 SV=1
MM1 pKa = 7.61 AFKK4 pKa = 10.51 FDD6 pKa = 3.95 ASNLNKK12 pKa = 9.24 TRR14 pKa = 11.84 QALATMSNPSFQPKK28 pKa = 9.85 GFPSNSTQFNGSNGRR43 pKa = 11.84 KK44 pKa = 9.62 GKK46 pKa = 10.53 FPDD49 pKa = 3.5 AKK51 pKa = 9.62 KK52 pKa = 7.26 TTLSSPPLFNEE63 pKa = 3.8 SQKK66 pKa = 10.77 PKK68 pKa = 9.31 TVSSMITSPIIGSDD82 pKa = 3.72 RR83 pKa = 11.84 IFTSSEE89 pKa = 3.91 FIHH92 pKa = 5.8 PQKK95 pKa = 10.74 FVMTEE100 pKa = 3.78 YY101 pKa = 9.69 VQKK104 pKa = 10.86 SIAKK108 pKa = 9.27 FEE110 pKa = 4.27 THH112 pKa = 6.7 GSIDD116 pKa = 3.83 PFAKK120 pKa = 10.52 KK121 pKa = 9.88 PDD123 pKa = 3.82 FDD125 pKa = 4.62 DD126 pKa = 4.19 LCLILYY132 pKa = 7.74 TPAASKK138 pKa = 11.08 HH139 pKa = 5.08 EE140 pKa = 4.18 LSLANAQLVCSDD152 pKa = 3.26 IDD154 pKa = 3.81 SLNLHH159 pKa = 6.14 SMHH162 pKa = 6.88 VIDD165 pKa = 4.04 GTKK168 pKa = 9.89 LYY170 pKa = 11.48 SMAANVQYY178 pKa = 11.25 LNAGVIYY185 pKa = 10.07 LPKK188 pKa = 9.96 SHH190 pKa = 7.1 WDD192 pKa = 3.02 AFTSKK197 pKa = 7.69 THH199 pKa = 5.79 AHH201 pKa = 5.92 VKK203 pKa = 9.76 MEE205 pKa = 4.27 MNFACSPVHH214 pKa = 6.54 LNKK217 pKa = 10.14 LRR219 pKa = 11.84 LAHH222 pKa = 6.17 WMSPFLLTKK231 pKa = 10.22 MSVSFQLQVEE241 pKa = 4.64 IYY243 pKa = 10.45 DD244 pKa = 3.65 HH245 pKa = 7.2 FEE247 pKa = 3.86 QFVKK251 pKa = 9.62 NTHH254 pKa = 6.47 ANVDD258 pKa = 3.17 SPMRR262 pKa = 11.84 VFGSKK267 pKa = 9.78 NGKK270 pKa = 8.78 EE271 pKa = 4.29 CGSLEE276 pKa = 4.14 PEE278 pKa = 4.34 EE279 pKa = 5.75 DD280 pKa = 3.68 LDD282 pKa = 5.06 SPLDD286 pKa = 3.71 FSKK289 pKa = 11.59 NNVCLIDD296 pKa = 3.82 VNDD299 pKa = 4.7 DD300 pKa = 3.26 FTSAEE305 pKa = 3.8 RR306 pKa = 11.84 KK307 pKa = 7.41 MHH309 pKa = 5.8 VNLNVFRR316 pKa = 11.84 NAITFVKK323 pKa = 10.59 KK324 pKa = 10.51 EE325 pKa = 4.07 FGRR328 pKa = 11.84 ANGGNSLVQKK338 pKa = 9.72 MRR340 pKa = 11.84 ASKK343 pKa = 10.9 VFLLYY348 pKa = 10.44 FDD350 pKa = 5.62 LNNTSEE356 pKa = 4.11 TRR358 pKa = 11.84 NRR360 pKa = 11.84 KK361 pKa = 8.49 QRR363 pKa = 11.84 DD364 pKa = 3.42 PSVVNTDD371 pKa = 3.3 TLKK374 pKa = 11.19 AFDD377 pKa = 4.02 GFNTVFVIVDD387 pKa = 3.95 SEE389 pKa = 4.33 GMIASSVSMSSVEE402 pKa = 4.08 AMLMSLKK409 pKa = 10.22 QVSLVDD415 pKa = 3.13 YY416 pKa = 10.13 HH417 pKa = 7.73 LNYY420 pKa = 10.65 CSDD423 pKa = 3.09 VLMNEE428 pKa = 3.77 GHH430 pKa = 6.55 VYY432 pKa = 11.03 SLMLSCICKK441 pKa = 8.67 TLSFGGRR448 pKa = 11.84 ILISSWIHH456 pKa = 5.89 ALFEE460 pKa = 4.6 GEE462 pKa = 4.8 KK463 pKa = 9.21 KK464 pKa = 8.67 TATEE468 pKa = 3.97 TFEE471 pKa = 4.07 EE472 pKa = 4.4 LKK474 pKa = 10.68 KK475 pKa = 11.23 LKK477 pKa = 10.45 DD478 pKa = 3.83 VEE480 pKa = 4.51 DD481 pKa = 4.2 KK482 pKa = 11.37 KK483 pKa = 10.76 PDD485 pKa = 3.05 AMTARR490 pKa = 11.84 KK491 pKa = 7.37 PTTDD495 pKa = 3.13 EE496 pKa = 4.56 NEE498 pKa = 4.58 DD499 pKa = 3.4 EE500 pKa = 4.45 NEE502 pKa = 4.0 AAKK505 pKa = 9.26 TAFDD509 pKa = 3.3 KK510 pKa = 11.43 HH511 pKa = 6.52 LDD513 pKa = 3.55 KK514 pKa = 11.47 VEE516 pKa = 4.9 KK517 pKa = 10.22 ILSEE521 pKa = 4.29 DD522 pKa = 4.24 SEE524 pKa = 4.66 NQDD527 pKa = 2.84 GTQTDD532 pKa = 3.7 TEE534 pKa = 4.18 EE535 pKa = 5.24 RR536 pKa = 11.84 LTQTTAAPNPIEE548 pKa = 4.31 TTFPAAQTQPDD559 pKa = 3.71 QTQKK563 pKa = 9.77 TEE565 pKa = 4.08 STDD568 pKa = 3.57 QNDD571 pKa = 3.89 SNVQDD576 pKa = 3.72 SQEE579 pKa = 4.06 NQSKK583 pKa = 10.83 DD584 pKa = 3.36 NQTRR588 pKa = 11.84 SHH590 pKa = 7.5 PLPDD594 pKa = 3.52 QDD596 pKa = 5.66 SVDD599 pKa = 4.34 DD600 pKa = 3.87 STKK603 pKa = 10.77 EE604 pKa = 3.79 SDD606 pKa = 3.56 ASSSATFDD614 pKa = 3.17 SATEE618 pKa = 4.08 FKK620 pKa = 10.32 EE621 pKa = 3.84 AANASVLVDD630 pKa = 3.92 LQDD633 pKa = 3.32 IFSNASSQTAQSSTTSSTKK652 pKa = 9.51 STSDD656 pKa = 2.78 QATFKK661 pKa = 10.32 NTVSKK666 pKa = 9.32 QTDD669 pKa = 3.24 SRR671 pKa = 11.84 SAFPDD676 pKa = 3.12 IQEE679 pKa = 4.41 KK680 pKa = 9.91 QLPAPPNSLDD690 pKa = 3.89 DD691 pKa = 5.16 GISASMGLPPPKK703 pKa = 10.06 IMTVDD708 pKa = 3.46 EE709 pKa = 4.36 VEE711 pKa = 4.08 AALFGTKK718 pKa = 9.99 EE719 pKa = 4.13 NGSVASPVKK728 pKa = 10.31 NVPIHH733 pKa = 6.27 EE734 pKa = 4.37 KK735 pKa = 10.68 ASPKK739 pKa = 9.51 NANGRR744 pKa = 11.84 TLPPVPEE751 pKa = 4.18 EE752 pKa = 4.06 VPIGNVGGPRR762 pKa = 11.84 QLKK765 pKa = 10.47 VSFDD769 pKa = 3.94 DD770 pKa = 6.41 LMDD773 pKa = 4.44 FSQNASAVQNSCPSNNIPPSPSEE796 pKa = 3.86 SSEE799 pKa = 4.01 TTKK802 pKa = 10.52 QQSSSCGSSNNPSSSTGYY820 pKa = 10.79 VGDD823 pKa = 3.8 NTKK826 pKa = 9.6 TEE828 pKa = 4.33 PLPTTPSPSDD838 pKa = 3.21 AASTKK843 pKa = 10.75 SVDD846 pKa = 3.41 NDD848 pKa = 3.7 ALSTPPNGNVDD859 pKa = 3.45 DD860 pKa = 4.96 HH861 pKa = 5.72 QAKK864 pKa = 10.41 SNPSSVFDD872 pKa = 4.08 FTASCQSSKK881 pKa = 11.02 SDD883 pKa = 4.13 SGLCTPTASTTSDD896 pKa = 3.23 PNQALFYY903 pKa = 10.28 QQFSFIKK910 pKa = 9.89 EE911 pKa = 4.15 YY912 pKa = 11.18 NCTPEE917 pKa = 3.88 NLRR920 pKa = 11.84 LLLEE924 pKa = 4.23 TVRR927 pKa = 11.84 ARR929 pKa = 11.84 LIPMHH934 pKa = 6.64 LVHH937 pKa = 7.08 PEE939 pKa = 3.77 VVPNLDD945 pKa = 3.75 SVCEE949 pKa = 3.96 PRR951 pKa = 11.84 NLSLAFGLSTLCYY964 pKa = 10.27 DD965 pKa = 4.19 PNPNTGCC972 pKa = 3.92
Molecular weight: 106.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.032
IPC2_protein 5.003
IPC_protein 4.991
Toseland 4.94
ProMoST 5.156
Dawson 5.003
Bjellqvist 5.13
Wikipedia 4.889
Rodwell 4.902
Grimsley 4.863
Solomon 5.003
Lehninger 4.965
Nozaki 5.13
DTASelect 5.308
Thurlkill 4.952
EMBOSS 4.94
Sillero 5.194
Patrickios 4.177
IPC_peptide 5.003
IPC2_peptide 5.181
IPC2.peptide.svr19 5.154
Protein with the highest isoelectric point:
>tr|Q83865|Q83865_9REOV 33KD putative nonstructural protein OS=Nilaparvata lugens reovirus OX=33724 PE=4 SV=1
MM1 pKa = 7.36 EE2 pKa = 5.3 HH3 pKa = 7.26 LEE5 pKa = 4.6 TITDD9 pKa = 5.05 LIFPIFINRR18 pKa = 11.84 FRR20 pKa = 11.84 QNKK23 pKa = 8.22 YY24 pKa = 9.32 NNRR27 pKa = 11.84 SNTKK31 pKa = 9.89 LLNKK35 pKa = 9.44 PSPVDD40 pKa = 3.0 IKK42 pKa = 10.71 RR43 pKa = 11.84 YY44 pKa = 7.49 YY45 pKa = 10.47 SSYY48 pKa = 9.95 NSQHH52 pKa = 6.1 AVLLRR57 pKa = 11.84 VTYY60 pKa = 8.97 MPHH63 pKa = 6.27 HH64 pKa = 6.22 EE65 pKa = 5.19 RR66 pKa = 11.84 YY67 pKa = 9.2 LQSLNIQDD75 pKa = 3.65 AQKK78 pKa = 10.68 RR79 pKa = 11.84 GYY81 pKa = 6.93 TAKK84 pKa = 10.72 NLDD87 pKa = 3.87 LMWYY91 pKa = 10.02 RR92 pKa = 11.84 LFTGTQRR99 pKa = 11.84 GIYY102 pKa = 8.89 YY103 pKa = 9.25 PPHH106 pKa = 5.87 VFNNITKK113 pKa = 10.08 KK114 pKa = 10.9 YY115 pKa = 9.52 GFGLIMNDD123 pKa = 2.59 IQTKK127 pKa = 10.35 VDD129 pKa = 4.01 LEE131 pKa = 4.63 TKK133 pKa = 7.49 NTHH136 pKa = 5.15 YY137 pKa = 11.07 VVVDD141 pKa = 3.87 FDD143 pKa = 3.88 EE144 pKa = 6.74 AEE146 pKa = 4.01 EE147 pKa = 4.2 SDD149 pKa = 5.04 NGIQNPGTIIQFSGNGIPMKK169 pKa = 10.63 YY170 pKa = 9.87 KK171 pKa = 10.74 SSTNKK176 pKa = 9.55 QFLNNIIKK184 pKa = 10.56 LEE186 pKa = 3.95 RR187 pKa = 11.84 KK188 pKa = 9.42 ILDD191 pKa = 3.14 EE192 pKa = 4.27 SKK194 pKa = 10.8 RR195 pKa = 11.84 VDD197 pKa = 3.71 PLALLNEE204 pKa = 5.04 SIQTEE209 pKa = 4.33 CAKK212 pKa = 11.04 GNVVKK217 pKa = 10.41 LQKK220 pKa = 10.49 RR221 pKa = 11.84 EE222 pKa = 4.34 DD223 pKa = 3.11 ISFAFEE229 pKa = 3.98 CYY231 pKa = 9.67 EE232 pKa = 3.8 YY233 pKa = 10.55 TGNYY237 pKa = 8.59 VKK239 pKa = 10.82 GNSFIKK245 pKa = 10.16 MLLNVDD251 pKa = 4.53 FTPIYY256 pKa = 7.93 YY257 pKa = 9.88 QYY259 pKa = 11.55 YY260 pKa = 8.39 LTDD263 pKa = 3.36 TTNEE267 pKa = 3.78 IEE269 pKa = 4.35 SYY271 pKa = 9.37 PVYY274 pKa = 10.38 VQSVLNRR281 pKa = 11.84 MKK283 pKa = 10.64 LVRR286 pKa = 11.84 VPKK289 pKa = 10.25 IEE291 pKa = 3.86 NEE293 pKa = 3.72 IMNGVMPTEE302 pKa = 4.36 IAISTCGIVNVRR314 pKa = 11.84 FNGWVFTNLAPRR326 pKa = 11.84 SKK328 pKa = 10.11 FRR330 pKa = 11.84 LYY332 pKa = 9.04 GTEE335 pKa = 4.07 KK336 pKa = 9.93 IKK338 pKa = 10.85 HH339 pKa = 6.17 FIMNYY344 pKa = 8.09 VSSFSEE350 pKa = 4.23 YY351 pKa = 10.71 QMVPYY356 pKa = 10.05 FEE358 pKa = 6.23 SGAEE362 pKa = 3.67 YY363 pKa = 10.37 TMRR366 pKa = 11.84 IGRR369 pKa = 11.84 PIATSFSTFSRR380 pKa = 11.84 EE381 pKa = 3.59 RR382 pKa = 11.84 MAEE385 pKa = 3.87 NFKK388 pKa = 10.99 DD389 pKa = 3.79 AVRR392 pKa = 11.84 EE393 pKa = 3.85 RR394 pKa = 11.84 MPIWVVANKK403 pKa = 10.55 GSGKK407 pKa = 8.23 TVLRR411 pKa = 11.84 KK412 pKa = 9.4 EE413 pKa = 4.02 LEE415 pKa = 4.19 EE416 pKa = 4.41 IGFNVIDD423 pKa = 3.68 SDD425 pKa = 4.09 AYY427 pKa = 8.96 GWFVSKK433 pKa = 10.41 VAYY436 pKa = 9.02 IHH438 pKa = 7.27 KK439 pKa = 9.88 EE440 pKa = 3.97 VNQNTLSTIEE450 pKa = 4.6 LSQDD454 pKa = 3.29 HH455 pKa = 6.91 INRR458 pKa = 11.84 LVSEE462 pKa = 4.28 VLEE465 pKa = 4.07 EE466 pKa = 4.3 DD467 pKa = 3.7 RR468 pKa = 11.84 GISYY472 pKa = 10.13 FNVIMYY478 pKa = 10.6 SLTKK482 pKa = 9.8 QKK484 pKa = 10.9 RR485 pKa = 11.84 DD486 pKa = 3.16 INKK489 pKa = 8.8 VLTFPYY495 pKa = 9.06 VTHH498 pKa = 6.96 RR499 pKa = 11.84 WNDD502 pKa = 3.17 TLTTFGKK509 pKa = 10.4 VFTEE513 pKa = 4.29 IADD516 pKa = 3.89 SPAIGYY522 pKa = 8.21 PRR524 pKa = 11.84 FIEE527 pKa = 4.58 GCMEE531 pKa = 3.89 YY532 pKa = 10.72 FRR534 pKa = 11.84 VRR536 pKa = 11.84 EE537 pKa = 4.08 TLHH540 pKa = 6.76 KK541 pKa = 10.17 PIIFFAHH548 pKa = 6.11 TEE550 pKa = 4.22 SEE552 pKa = 4.1 LSRR555 pKa = 11.84 ISIRR559 pKa = 11.84 HH560 pKa = 5.27 FSCVIYY566 pKa = 10.86 NSIDD570 pKa = 3.28 SRR572 pKa = 11.84 SILSKK577 pKa = 10.41 RR578 pKa = 11.84 ADD580 pKa = 3.39 PVVEE584 pKa = 4.43 LMLHH588 pKa = 6.62 AYY590 pKa = 10.68 YY591 pKa = 11.34 YY592 pKa = 8.74 MNQVAIDD599 pKa = 4.65 KK600 pKa = 10.71 IPFSLFRR607 pKa = 11.84 QWLGMSNKK615 pKa = 9.26 VVDD618 pKa = 4.54 EE619 pKa = 4.42 SVVAQLIVRR628 pKa = 11.84 EE629 pKa = 4.23
Molecular weight: 73.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.062
IPC2_protein 8.2
IPC_protein 8.097
Toseland 8.448
ProMoST 8.536
Dawson 8.887
Bjellqvist 8.829
Wikipedia 9.063
Rodwell 8.975
Grimsley 8.843
Solomon 8.96
Lehninger 8.916
Nozaki 8.799
DTASelect 8.741
Thurlkill 8.785
EMBOSS 9.004
Sillero 8.975
Patrickios 4.202
IPC_peptide 8.946
IPC2_peptide 7.541
IPC2.peptide.svr19 7.664
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
8916
206
1442
810.5
92.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.957 ± 0.293
1.447 ± 0.196
6.18 ± 0.302
5.002 ± 0.366
5.316 ± 0.239
4.565 ± 0.25
2.131 ± 0.146
7.279 ± 0.511
6.292 ± 0.262
8.636 ± 0.408
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.838 ± 0.099
6.819 ± 0.239
3.892 ± 0.312
3.297 ± 0.174
4.307 ± 0.339
8.457 ± 0.523
6.886 ± 0.242
6.359 ± 0.251
0.729 ± 0.073
4.61 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here