Fusibacter sp. A1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Eubacteriales Family XII. Incertae Sedis; Fusibacter; unclassified Fusibacter

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3729 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q1ZTF1|A0A4Q1ZTF1_9FIRM Ribonuclease PH OS=Fusibacter sp. A1 OX=2283630 GN=rph PE=3 SV=1
MM1 pKa = 7.31KK2 pKa = 10.01RR3 pKa = 11.84HH4 pKa = 5.56FKK6 pKa = 9.87VRR8 pKa = 11.84HH9 pKa = 4.76VFSFVLILALVMAGVACQKK28 pKa = 10.34QLSAKK33 pKa = 8.02EE34 pKa = 4.17TVEE37 pKa = 3.83RR38 pKa = 11.84LYY40 pKa = 11.08EE41 pKa = 3.92EE42 pKa = 4.55SLNLDD47 pKa = 3.48AYY49 pKa = 9.35SAKK52 pKa = 10.6FEE54 pKa = 4.79GDD56 pKa = 2.77MSMEE60 pKa = 5.1FIGEE64 pKa = 4.22TDD66 pKa = 3.45PMIEE70 pKa = 4.09QYY72 pKa = 11.47LGMFKK77 pKa = 10.31NIKK80 pKa = 9.43MSGDD84 pKa = 3.48MQYY87 pKa = 11.48KK88 pKa = 8.83DD89 pKa = 3.24TDD91 pKa = 4.16RR92 pKa = 11.84IGDD95 pKa = 3.49YY96 pKa = 11.54AMNYY100 pKa = 7.98TLDD103 pKa = 3.75MNGMSMAIEE112 pKa = 4.98FYY114 pKa = 10.86FDD116 pKa = 3.61GSRR119 pKa = 11.84MIVNYY124 pKa = 9.49PLLPNYY130 pKa = 9.69IVIDD134 pKa = 4.13LPEE137 pKa = 4.79LLGMLNEE144 pKa = 4.1EE145 pKa = 3.97QEE147 pKa = 4.53APVEE151 pKa = 3.84LDD153 pKa = 3.31YY154 pKa = 11.69EE155 pKa = 4.94SIVQDD160 pKa = 4.09LEE162 pKa = 4.19ALTSEE167 pKa = 4.75FMPKK171 pKa = 10.45LSGEE175 pKa = 4.12IIKK178 pKa = 11.0LIDD181 pKa = 3.78EE182 pKa = 4.74NSLEE186 pKa = 4.39LLDD189 pKa = 5.23EE190 pKa = 4.33YY191 pKa = 11.16TFTVDD196 pKa = 4.05GEE198 pKa = 4.92SVTSKK203 pKa = 10.61AVKK206 pKa = 10.53VNLDD210 pKa = 3.47TDD212 pKa = 3.94LMIGMMNSLFEE223 pKa = 3.86QAKK226 pKa = 9.07NSEE229 pKa = 4.2VVYY232 pKa = 10.19QIVKK236 pKa = 10.42KK237 pKa = 10.85YY238 pKa = 10.92DD239 pKa = 3.09IDD241 pKa = 4.71DD242 pKa = 3.88EE243 pKa = 4.68VGSFEE248 pKa = 5.75DD249 pKa = 3.86YY250 pKa = 10.67QAGFDD255 pKa = 3.67GLKK258 pKa = 10.63ASFEE262 pKa = 4.32EE263 pKa = 4.31EE264 pKa = 4.04MNLSEE269 pKa = 5.16FEE271 pKa = 4.28EE272 pKa = 4.26MMKK275 pKa = 10.58NISYY279 pKa = 8.96TYY281 pKa = 10.45ILGYY285 pKa = 10.3DD286 pKa = 2.83KK287 pKa = 11.02NYY289 pKa = 10.4RR290 pKa = 11.84VNHH293 pKa = 5.7MALDD297 pKa = 4.02LTMKK301 pKa = 10.13IDD303 pKa = 4.1DD304 pKa = 4.18EE305 pKa = 5.18SIGTEE310 pKa = 3.38IEE312 pKa = 3.98MTMGAVYY319 pKa = 10.92AMNYY323 pKa = 8.08DD324 pKa = 3.32AKK326 pKa = 10.59EE327 pKa = 3.82IEE329 pKa = 4.49FPEE332 pKa = 4.33LTDD335 pKa = 3.78EE336 pKa = 4.2NSMSIMEE343 pKa = 5.12LFPDD347 pKa = 3.93LSGYY351 pKa = 10.66GSYY354 pKa = 11.05EE355 pKa = 4.03DD356 pKa = 3.87GGDD359 pKa = 3.62YY360 pKa = 10.95EE361 pKa = 6.14DD362 pKa = 4.48YY363 pKa = 11.5VPVNYY368 pKa = 10.24FIEE371 pKa = 4.5EE372 pKa = 4.03QVLMLDD378 pKa = 6.11DD379 pKa = 4.22IAQDD383 pKa = 3.58LTLLTEE389 pKa = 3.99TTEE392 pKa = 3.81IEE394 pKa = 4.44EE395 pKa = 4.24VFEE398 pKa = 4.61SYY400 pKa = 11.17VYY402 pKa = 10.73DD403 pKa = 3.69SYY405 pKa = 11.88FEE407 pKa = 4.15VMNLYY412 pKa = 10.2YY413 pKa = 9.92ATSEE417 pKa = 4.44GEE419 pKa = 4.07MILYY423 pKa = 8.73PNQEE427 pKa = 4.2LPDD430 pKa = 3.85NYY432 pKa = 10.78DD433 pKa = 3.01PRR435 pKa = 11.84EE436 pKa = 4.08RR437 pKa = 11.84PWYY440 pKa = 9.62TEE442 pKa = 3.75ALLQGTMIGEE452 pKa = 4.73LYY454 pKa = 10.42EE455 pKa = 4.57DD456 pKa = 3.86AASGRR461 pKa = 11.84FVQSISKK468 pKa = 9.03TIVLDD473 pKa = 3.8GEE475 pKa = 4.69VIGVVGIDD483 pKa = 3.3IFVDD487 pKa = 3.67

Molecular weight: 55.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q1ZTC1|A0A4Q1ZTC1_9FIRM DUF1846 domain-containing protein OS=Fusibacter sp. A1 OX=2283630 GN=DWB64_14555 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 8.57KK9 pKa = 7.61RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.25RR14 pKa = 11.84EE15 pKa = 3.66HH16 pKa = 6.26GFRR19 pKa = 11.84KK20 pKa = 9.93RR21 pKa = 11.84MSSSAGRR28 pKa = 11.84NVLKK32 pKa = 10.44RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84NKK41 pKa = 10.5LSAA44 pKa = 3.94

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3729

0

3729

1207282

31

2347

323.8

36.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.73 ± 0.042

0.889 ± 0.015

5.958 ± 0.032

7.175 ± 0.04

4.382 ± 0.029

6.616 ± 0.041

2.123 ± 0.02

8.07 ± 0.035

6.84 ± 0.03

9.934 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.076 ± 0.019

4.331 ± 0.024

3.025 ± 0.023

2.996 ± 0.022

3.991 ± 0.026

6.401 ± 0.032

5.474 ± 0.034

7.317 ± 0.026

0.806 ± 0.015

3.866 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski