Bat mastadenovirus
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G9EAM2|A0A3G9EAM2_9ADEN Pre-hexon-linking protein IIIa OS=Bat mastadenovirus OX=740971 PE=2 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.44 VFTVPPSFDD11 pKa = 3.36 LEE13 pKa = 4.42 NYY15 pKa = 9.23 VQGLLDD21 pKa = 4.29 DD22 pKa = 4.4 WRR24 pKa = 11.84 PDD26 pKa = 3.3 CCLGEE31 pKa = 4.19 PCSRR35 pKa = 11.84 SPSPPSLYY43 pKa = 10.65 EE44 pKa = 4.32 LFDD47 pKa = 3.79 LTPPASPVLPCVPAAPDD64 pKa = 3.98 SPPSSPVLCDD74 pKa = 3.16 DD75 pKa = 5.05 CYY77 pKa = 11.26 FEE79 pKa = 6.32 ADD81 pKa = 3.75 EE82 pKa = 4.83 VLSPCCTEE90 pKa = 3.87 VSSTTEE96 pKa = 3.84 VLSTPSVSPIPTPVLDD112 pKa = 3.69 PTITEE117 pKa = 3.97 EE118 pKa = 4.02 MLLCLEE124 pKa = 4.7 EE125 pKa = 4.45 MPSFDD130 pKa = 5.83 DD131 pKa = 3.57 EE132 pKa = 5.03 DD133 pKa = 4.02 EE134 pKa = 4.41 VRR136 pKa = 11.84 SSSSSFEE143 pKa = 3.89 HH144 pKa = 6.43 WSGSLEE150 pKa = 4.18 PASGPSMGCLRR161 pKa = 11.84 CAFYY165 pKa = 10.55 QDD167 pKa = 3.24 RR168 pKa = 11.84 GEE170 pKa = 4.51 ASLCGLCYY178 pKa = 10.45 LKK180 pKa = 10.81 ALSEE184 pKa = 4.8 VPFSMPKK191 pKa = 9.76 RR192 pKa = 11.84 SGAAEE197 pKa = 4.12 EE198 pKa = 4.55 EE199 pKa = 4.69 VSSSEE204 pKa = 3.82 SDD206 pKa = 3.69 VIFVGQSKK214 pKa = 10.41 RR215 pKa = 11.84 KK216 pKa = 8.52 SPCGFVSPAKK226 pKa = 9.85 RR227 pKa = 11.84 QKK229 pKa = 9.33 TDD231 pKa = 2.79 EE232 pKa = 4.43 PLPVQTEE239 pKa = 4.01 PLDD242 pKa = 4.45 LSIKK246 pKa = 9.63 PRR248 pKa = 11.84 PEE250 pKa = 3.33
Molecular weight: 27.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.855
IPC2_protein 4.126
IPC_protein 4.075
Toseland 3.897
ProMoST 4.164
Dawson 4.024
Bjellqvist 4.202
Wikipedia 3.91
Rodwell 3.91
Grimsley 3.808
Solomon 4.024
Lehninger 3.973
Nozaki 4.139
DTASelect 4.291
Thurlkill 3.91
EMBOSS 3.923
Sillero 4.19
Patrickios 1.252
IPC_peptide 4.024
IPC2_peptide 4.177
IPC2.peptide.svr19 4.085
Protein with the highest isoelectric point:
>tr|A0A3G9EWN5|A0A3G9EWN5_9ADEN U exon OS=Bat mastadenovirus OX=740971 PE=4 SV=1
MM1 pKa = 7.28 ARR3 pKa = 11.84 KK4 pKa = 9.29 NVTLRR9 pKa = 11.84 LRR11 pKa = 11.84 LPVRR15 pKa = 11.84 TRR17 pKa = 11.84 LSGTGRR23 pKa = 11.84 RR24 pKa = 11.84 PRR26 pKa = 11.84 RR27 pKa = 11.84 TRR29 pKa = 11.84 GMRR32 pKa = 11.84 CGRR35 pKa = 11.84 MKK37 pKa = 10.87 GGFLPALIPLIAAAIGAVPGIASVALQAARR67 pKa = 11.84 HH68 pKa = 4.96
Molecular weight: 7.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.452
IPC2_protein 10.979
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.735
Wikipedia 13.217
Rodwell 12.31
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.735
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.047
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.132
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26
4
30
10069
55
1138
335.6
37.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.737 ± 0.347
1.986 ± 0.357
4.837 ± 0.198
5.909 ± 0.491
4.171 ± 0.195
5.691 ± 0.31
2.096 ± 0.19
4.678 ± 0.204
4.648 ± 0.419
9.385 ± 0.39
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.473 ± 0.203
5.115 ± 0.396
6.336 ± 0.331
4.4 ± 0.241
5.879 ± 0.529
7.091 ± 0.298
6.277 ± 0.334
6.555 ± 0.298
1.232 ± 0.091
3.506 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here