Bordetella phage BPP-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Rauchvirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q775D6|FIBD_BPBPP Tail fiber receptor-binding protein OS=Bordetella phage BPP-1 OX=194699 GN=mtd PE=1 SV=1
MM1 pKa = 7.43SVQSTDD7 pKa = 3.4RR8 pKa = 11.84VAGPYY13 pKa = 9.06PCNGLTTQFPFDD25 pKa = 3.57FKK27 pKa = 11.36VFNTGEE33 pKa = 4.18VVAILSDD40 pKa = 3.62ADD42 pKa = 3.89GVEE45 pKa = 4.37STLTLGTDD53 pKa = 3.39YY54 pKa = 10.94TVALNDD60 pKa = 3.95NQDD63 pKa = 3.85SNPGGTLTTLATYY76 pKa = 8.18ATGYY80 pKa = 10.5KK81 pKa = 8.59ITLTSEE87 pKa = 4.27VPNTQPEE94 pKa = 4.44TLTNQGGFYY103 pKa = 10.28PKK105 pKa = 10.33VIEE108 pKa = 4.35HH109 pKa = 6.67ALDD112 pKa = 3.43RR113 pKa = 11.84LVIQIQQLADD123 pKa = 3.29KK124 pKa = 10.85LSRR127 pKa = 11.84TVQAPISGGLSSSEE141 pKa = 3.79ILEE144 pKa = 4.08QLAEE148 pKa = 3.98QLPLVSAVYY157 pKa = 10.08GHH159 pKa = 7.72LANIDD164 pKa = 3.41AVATNEE170 pKa = 3.85ADD172 pKa = 3.26IDD174 pKa = 4.04TVAASVGAVDD184 pKa = 4.66TVAGDD189 pKa = 4.96LGGTWAAGVSYY200 pKa = 10.67DD201 pKa = 3.95FGSIAVPPIGNTSPPGGNIVIVANSIGNVDD231 pKa = 3.7TVAEE235 pKa = 4.36NIGDD239 pKa = 3.65VSTVSTHH246 pKa = 6.03LSSMLAVANDD256 pKa = 3.24IDD258 pKa = 4.51SVVSVAGDD266 pKa = 3.74LEE268 pKa = 5.05NIDD271 pKa = 4.68AVADD275 pKa = 3.53NAANINTVAGANANVNTVASNILDD299 pKa = 3.67VGTVAGNIDD308 pKa = 4.01DD309 pKa = 4.26VQAVAGNAANINVVADD325 pKa = 3.47NADD328 pKa = 4.11NINATAANQANINAAVGNADD348 pKa = 4.1NINAAVANQANINAVVGNANNINAVAANEE377 pKa = 4.45GNVNTVVDD385 pKa = 4.14NLADD389 pKa = 3.71VQTVAGIAADD399 pKa = 3.52VSTVAEE405 pKa = 4.08NEE407 pKa = 4.0AAVAALGNDD416 pKa = 3.46LTGQPMVIDD425 pKa = 4.19YY426 pKa = 11.11GDD428 pKa = 4.81LSPASNPAAPAGVLGAVWANAEE450 pKa = 4.1NIAAVAEE457 pKa = 4.37NIAVIIEE464 pKa = 4.07AANNLPAILAALSGALVPANNLSDD488 pKa = 4.1LADD491 pKa = 3.99PAAARR496 pKa = 11.84ANIGLADD503 pKa = 4.26LGGIAA508 pKa = 5.16

Molecular weight: 50.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q775A7|Q775A7_BPBPP Bbp38 OS=Bordetella phage BPP-1 OX=194699 GN=bbp38 PE=4 SV=1
MM1 pKa = 7.82KK2 pKa = 10.28NRR4 pKa = 11.84QPVQTTLPPEE14 pKa = 4.23AMRR17 pKa = 11.84LLQQAAQTPITRR29 pKa = 11.84ADD31 pKa = 3.25PLARR35 pKa = 11.84VKK37 pKa = 10.7AIEE40 pKa = 4.12KK41 pKa = 8.44ATEE44 pKa = 3.94RR45 pKa = 11.84VKK47 pKa = 10.66RR48 pKa = 11.84QYY50 pKa = 10.99PHH52 pKa = 6.84FFKK55 pKa = 10.82EE56 pKa = 4.04

Molecular weight: 6.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

13651

56

2052

278.6

30.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.699 ± 0.538

0.769 ± 0.165

6.38 ± 0.241

5.685 ± 0.288

3.143 ± 0.146

8.241 ± 0.358

1.795 ± 0.17

3.978 ± 0.208

4.168 ± 0.276

8.556 ± 0.352

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.212 ± 0.151

3.443 ± 0.389

4.688 ± 0.227

4.498 ± 0.436

6.637 ± 0.344

4.959 ± 0.261

6.102 ± 0.331

6.659 ± 0.312

1.641 ± 0.151

2.747 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski