Acinetobacter sp. ANC 4999

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Acinetobacter; unclassified Acinetobacter

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2794 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1Y3CEN1|A0A1Y3CEN1_9GAMM DNA-binding protein HU OS=Acinetobacter sp. ANC 4999 OX=1977882 GN=B9T28_09810 PE=3 SV=1
MM1 pKa = 7.7KK2 pKa = 10.11KK3 pKa = 9.69YY4 pKa = 10.65QCIVCGWIYY13 pKa = 11.09DD14 pKa = 4.04EE15 pKa = 5.55AEE17 pKa = 3.9GWPQDD22 pKa = 3.97GIAAGTKK29 pKa = 9.28WDD31 pKa = 5.81DD32 pKa = 4.6IPDD35 pKa = 3.76DD36 pKa = 4.19WTCPDD41 pKa = 4.02CGVSKK46 pKa = 11.14VDD48 pKa = 3.99FEE50 pKa = 4.42MVEE53 pKa = 3.84II54 pKa = 4.69

Molecular weight:
6.16 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1Y3C6L8|A0A1Y3C6L8_9GAMM Uncharacterized protein OS=Acinetobacter sp. ANC 4999 OX=1977882 GN=B9T28_14260 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.55RR3 pKa = 11.84TFQPSEE9 pKa = 3.97LKK11 pKa = 10.13RR12 pKa = 11.84KK13 pKa = 8.98RR14 pKa = 11.84VHH16 pKa = 6.36GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4AGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84HH40 pKa = 5.1SLTVV44 pKa = 3.06

Molecular weight:
5.18 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2794

0

2794

873642

19

3994

312.7

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.54 ± 0.049

0.936 ± 0.014

5.211 ± 0.041

5.74 ± 0.048

4.283 ± 0.038

6.53 ± 0.071

2.398 ± 0.027

7.147 ± 0.041

5.742 ± 0.045

10.372 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.564 ± 0.028

4.673 ± 0.059

3.803 ± 0.031

5.406 ± 0.052

4.112 ± 0.039

6.148 ± 0.038

5.42 ± 0.049

6.549 ± 0.043

1.223 ± 0.018

3.201 ± 0.029

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski