Plasticicumulans lactativorans
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3868 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R2L2Z5|A0A4R2L2Z5_9GAMM Uncharacterized protein (TIGR00369 family) OS=Plasticicumulans lactativorans OX=1133106 GN=EV699_11165 PE=4 SV=1
MM1 pKa = 7.48 SNIPSDD7 pKa = 3.96 LLYY10 pKa = 10.8 ASSHH14 pKa = 4.0 EE15 pKa = 4.15 WVRR18 pKa = 11.84 QEE20 pKa = 3.7 EE21 pKa = 4.5 DD22 pKa = 3.35 GTVTVGISDD31 pKa = 4.04 HH32 pKa = 6.49 AQTALGDD39 pKa = 3.76 LVYY42 pKa = 11.12 VEE44 pKa = 4.47 VPEE47 pKa = 4.43 AGRR50 pKa = 11.84 EE51 pKa = 4.06 VEE53 pKa = 4.09 AAEE56 pKa = 4.12 ACAVVEE62 pKa = 4.41 SVKK65 pKa = 10.67 AASDD69 pKa = 3.76 VYY71 pKa = 11.29 APIAGEE77 pKa = 4.1 VVEE80 pKa = 5.93 ANAQLADD87 pKa = 3.62 QPEE90 pKa = 4.77 LVNQDD95 pKa = 3.29 PYY97 pKa = 10.6 GAGWLFRR104 pKa = 11.84 LRR106 pKa = 11.84 PSGGVDD112 pKa = 3.13 GLLSAEE118 pKa = 4.52 AYY120 pKa = 9.58 AATLADD126 pKa = 3.55
Molecular weight: 13.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.91
IPC_protein 3.834
Toseland 3.656
ProMoST 3.923
Dawson 3.808
Bjellqvist 4.012
Wikipedia 3.706
Rodwell 3.668
Grimsley 3.567
Solomon 3.795
Lehninger 3.745
Nozaki 3.935
DTASelect 4.075
Thurlkill 3.694
EMBOSS 3.719
Sillero 3.948
Patrickios 1.863
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.896
Protein with the highest isoelectric point:
>tr|A0A4R2L5Z3|A0A4R2L5Z3_9GAMM Uncharacterized protein DUF3108 OS=Plasticicumulans lactativorans OX=1133106 GN=EV699_11232 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLHH11 pKa = 6.3 RR12 pKa = 11.84 KK13 pKa = 6.84 RR14 pKa = 11.84 THH16 pKa = 5.74 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.38 NGRR28 pKa = 11.84 LVIKK32 pKa = 10.35 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.21 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTPP44 pKa = 3.93
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3868
0
3868
1298026
25
2898
335.6
36.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.402 ± 0.068
1.0 ± 0.012
5.485 ± 0.033
5.514 ± 0.034
3.414 ± 0.023
8.687 ± 0.034
2.325 ± 0.018
3.968 ± 0.035
2.228 ± 0.029
11.662 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.888 ± 0.02
2.117 ± 0.024
5.576 ± 0.039
3.085 ± 0.023
8.172 ± 0.044
4.295 ± 0.026
4.726 ± 0.022
7.765 ± 0.039
1.426 ± 0.017
2.268 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here