Podoviridae sp. ctLUJ1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; unclassified Schitoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W8N1|A0A5Q2W8N1_9CAUD DNA-directed RNA polymerase subunit alpha OS=Podoviridae sp. ctLUJ1 OX=2656710 PE=4 SV=1
MM1 pKa = 7.77FYY3 pKa = 10.55TRR5 pKa = 11.84IEE7 pKa = 4.1FLPLNEE13 pKa = 4.19GEE15 pKa = 3.88IFYY18 pKa = 10.65FIMLFFWCFSSEE30 pKa = 4.06FF31 pKa = 3.7

Molecular weight: 3.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W3M6|A0A5Q2W3M6_9CAUD Uncharacterized protein OS=Podoviridae sp. ctLUJ1 OX=2656710 PE=4 SV=1
MM1 pKa = 7.17VGSFFLPVSAARR13 pKa = 11.84SLRR16 pKa = 11.84AADD19 pKa = 3.51SAKK22 pKa = 10.61QKK24 pKa = 8.71GTKK27 pKa = 8.92EE28 pKa = 4.16KK29 pKa = 10.93YY30 pKa = 10.41LSDD33 pKa = 3.63SNKK36 pKa = 9.75PNRR39 pKa = 3.89

Molecular weight: 4.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

22778

29

3446

271.2

30.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.701 ± 0.638

0.922 ± 0.16

5.598 ± 0.124

6.765 ± 0.238

3.639 ± 0.196

5.778 ± 0.273

1.901 ± 0.124

6.528 ± 0.276

6.954 ± 0.27

8.93 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.586 ± 0.147

5.562 ± 0.133

3.749 ± 0.187

3.648 ± 0.238

3.898 ± 0.152

6.634 ± 0.332

6.818 ± 0.291

6.761 ± 0.16

1.005 ± 0.134

3.622 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski