Synechococcus sp. (strain ATCC 29403 / PCC 7335)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5475 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4WTC7|B4WTC7_SYNS7 Uncharacterized protein OS=Synechococcus sp. (strain ATCC 29403 / PCC 7335) OX=91464 GN=S7335_137 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 9.19RR3 pKa = 11.84TTSLASARR11 pKa = 11.84FSGVCLATAALAALPLSAFAQQLPDD36 pKa = 3.43YY37 pKa = 10.06SYY39 pKa = 11.49VGLGGGDD46 pKa = 3.64DD47 pKa = 3.74GFVINSKK54 pKa = 8.38ITLGDD59 pKa = 3.37NFSIRR64 pKa = 11.84PSVATDD70 pKa = 3.56FDD72 pKa = 5.1FDD74 pKa = 4.28DD75 pKa = 5.18SEE77 pKa = 4.49DD78 pKa = 3.58VSYY81 pKa = 11.15LLPITYY87 pKa = 10.2DD88 pKa = 3.5LNAVDD93 pKa = 3.91GGGRR97 pKa = 11.84VYY99 pKa = 10.78PFVGAGLGGDD109 pKa = 4.36LGDD112 pKa = 5.49DD113 pKa = 3.67STIDD117 pKa = 3.54FAITGGVDD125 pKa = 3.04YY126 pKa = 10.88RR127 pKa = 11.84FANRR131 pKa = 11.84WVANGAVNYY140 pKa = 10.62LPFADD145 pKa = 4.03GDD147 pKa = 4.01EE148 pKa = 4.5VDD150 pKa = 4.38FSLGVGYY157 pKa = 8.19TFGGSYY163 pKa = 10.71

Molecular weight: 17.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4WJQ9|B4WJQ9_SYNS7 Uncharacterized protein OS=Synechococcus sp. (strain ATCC 29403 / PCC 7335) OX=91464 GN=S7335_3306 PE=4 SV=1
MM1 pKa = 7.4VKK3 pKa = 10.0RR4 pKa = 11.84SHH6 pKa = 6.63IAATRR11 pKa = 11.84LQLTRR16 pKa = 11.84LRR18 pKa = 11.84LISHH22 pKa = 7.11QINQLLGLMRR32 pKa = 11.84LLMMISRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84SGKK46 pKa = 9.91LL47 pKa = 3.05

Molecular weight: 5.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5475

0

5475

1624882

29

2932

296.8

32.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.208 ± 0.039

0.975 ± 0.012

5.465 ± 0.034

6.16 ± 0.038

3.797 ± 0.025

6.99 ± 0.047

1.875 ± 0.021

5.948 ± 0.028

4.02 ± 0.032

10.745 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.0 ± 0.018

3.485 ± 0.025

4.719 ± 0.027

4.962 ± 0.035

5.564 ± 0.034

7.154 ± 0.038

5.825 ± 0.025

6.901 ± 0.028

1.376 ± 0.017

2.827 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski