Amino acid dipepetide frequency for Synechococcus sp. (strain ATCC 29403 / PCC 7335)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.379AlaAla: 9.379 ± 0.095	0.901AlaCys: 0.901 ± 0.024	5.141AlaAsp: 5.141 ± 0.065	5.804AlaGlu: 5.804 ± 0.072	3.333AlaPhe: 3.333 ± 0.05	6.124AlaGly: 6.124 ± 0.086	1.578AlaHis: 1.578 ± 0.031	6.847AlaIle: 6.847 ± 0.066	3.974AlaLys: 3.974 ± 0.057	10.115AlaLeu: 10.115 ± 0.098	2.02AlaMet: 2.02 ± 0.036	3.203AlaAsn: 3.203 ± 0.049	3.411AlaPro: 3.411 ± 0.056	4.775AlaGln: 4.775 ± 0.055	4.313AlaArg: 4.313 ± 0.052	5.951AlaSer: 5.951 ± 0.064	5.205AlaThr: 5.205 ± 0.057	6.475AlaVal: 6.475 ± 0.078	1.02AlaTrp: 1.02 ± 0.027	2.513AlaTyr: 2.513 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.718CysAla: 0.718 ± 0.021	0.179CysCys: 0.179 ± 0.011	0.631CysAsp: 0.631 ± 0.021	0.561CysGlu: 0.561 ± 0.018	0.454CysPhe: 0.454 ± 0.019	0.763CysGly: 0.763 ± 0.021	0.292CysHis: 0.292 ± 0.015	0.519CysIle: 0.519 ± 0.019	0.319CysLys: 0.319 ± 0.014	1.124CysLeu: 1.124 ± 0.029	0.176CysMet: 0.176 ± 0.011	0.289CysAsn: 0.289 ± 0.013	0.467CysPro: 0.467 ± 0.017	0.527CysGln: 0.527 ± 0.018	0.54CysArg: 0.54 ± 0.017	0.66CysSer: 0.66 ± 0.022	0.451CysThr: 0.451 ± 0.016	0.594CysVal: 0.594 ± 0.018	0.145CysTrp: 0.145 ± 0.009	0.335CysTyr: 0.335 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	4.293AspAla: 4.293 ± 0.054	0.564AspCys: 0.564 ± 0.02	2.875AspAsp: 2.875 ± 0.066	3.314AspGlu: 3.314 ± 0.064	2.412AspPhe: 2.412 ± 0.044	3.928AspGly: 3.928 ± 0.102	0.923AspHis: 0.923 ± 0.028	3.565AspIle: 3.565 ± 0.057	1.912AspLys: 1.912 ± 0.041	5.935AspLeu: 5.935 ± 0.08	0.997AspMet: 0.997 ± 0.028	1.798AspAsn: 1.798 ± 0.036	2.713AspPro: 2.713 ± 0.05	2.411AspGln: 2.411 ± 0.042	3.811AspArg: 3.811 ± 0.052	3.716AspSer: 3.716 ± 0.049	2.989AspThr: 2.989 ± 0.059	3.5AspVal: 3.5 ± 0.055	0.955AspTrp: 0.955 ± 0.027	2.037AspTyr: 2.037 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	6.338GluAla: 6.338 ± 0.071	0.415GluCys: 0.415 ± 0.017	3.221GluAsp: 3.221 ± 0.052	3.872GluGlu: 3.872 ± 0.07	1.953GluPhe: 1.953 ± 0.034	3.818GluGly: 3.818 ± 0.052	1.038GluHis: 1.038 ± 0.031	3.912GluIle: 3.912 ± 0.047	2.857GluLys: 2.857 ± 0.046	6.301GluLeu: 6.301 ± 0.069	1.366GluMet: 1.366 ± 0.028	2.209GluAsn: 2.209 ± 0.038	2.739GluPro: 2.739 ± 0.053	3.532GluGln: 3.532 ± 0.055	3.743GluArg: 3.743 ± 0.057	3.99GluSer: 3.99 ± 0.057	3.858GluThr: 3.858 ± 0.055	4.3GluVal: 4.3 ± 0.058	0.735GluTrp: 0.735 ± 0.021	1.406GluTyr: 1.406 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	3.046PheAla: 3.046 ± 0.047	0.526PheCys: 0.526 ± 0.019	2.52PheAsp: 2.52 ± 0.046	2.518PheGlu: 2.518 ± 0.04	1.646PhePhe: 1.646 ± 0.038	3.118PheGly: 3.118 ± 0.057	0.693PheHis: 0.693 ± 0.02	1.945PheIle: 1.945 ± 0.039	1.335PheLys: 1.335 ± 0.033	3.754PheLeu: 3.754 ± 0.056	0.651PheMet: 0.651 ± 0.019	1.438PheAsn: 1.438 ± 0.03	1.645PhePro: 1.645 ± 0.033	1.453PheGln: 1.453 ± 0.033	1.856PheArg: 1.856 ± 0.035	3.182PheSer: 3.182 ± 0.046	2.086PheThr: 2.086 ± 0.033	2.702PheVal: 2.702 ± 0.054	0.645PheTrp: 0.645 ± 0.022	1.211PheTyr: 1.211 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	5.539GlyAla: 5.539 ± 0.082	0.77GlyCys: 0.77 ± 0.021	4.032GlyAsp: 4.032 ± 0.079	4.24GlyGlu: 4.24 ± 0.064	3.093GlyPhe: 3.093 ± 0.06	5.136GlyGly: 5.136 ± 0.091	1.366GlyHis: 1.366 ± 0.034	4.671GlyIle: 4.671 ± 0.061	3.221GlyLys: 3.221 ± 0.047	7.576GlyLeu: 7.576 ± 0.071	1.574GlyMet: 1.574 ± 0.035	2.697GlyAsn: 2.697 ± 0.065	2.081GlyPro: 2.081 ± 0.037	3.16GlyGln: 3.16 ± 0.046	3.736GlyArg: 3.736 ± 0.059	4.718GlySer: 4.718 ± 0.072	4.003GlyThr: 4.003 ± 0.061	4.7GlyVal: 4.7 ± 0.06	1.167GlyTrp: 1.167 ± 0.028	2.417GlyTyr: 2.417 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	1.273HisAla: 1.273 ± 0.027	0.262HisCys: 0.262 ± 0.014	0.823HisAsp: 0.823 ± 0.024	0.923HisGlu: 0.923 ± 0.024	0.826HisPhe: 0.826 ± 0.022	1.163HisGly: 1.163 ± 0.025	0.66HisHis: 0.66 ± 0.025	1.028HisIle: 1.028 ± 0.024	0.663HisLys: 0.663 ± 0.019	2.187HisLeu: 2.187 ± 0.043	0.308HisMet: 0.308 ± 0.013	0.636HisAsn: 0.636 ± 0.023	1.281HisPro: 1.281 ± 0.029	1.101HisGln: 1.101 ± 0.029	1.306HisArg: 1.306 ± 0.028	1.353HisSer: 1.353 ± 0.035	1.025HisThr: 1.025 ± 0.028	0.935HisVal: 0.935 ± 0.025	0.311HisTrp: 0.311 ± 0.013	0.684HisTyr: 0.684 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.105IleAla: 7.105 ± 0.077	0.66IleCys: 0.66 ± 0.018	3.897IleAsp: 3.897 ± 0.055	4.568IleGlu: 4.568 ± 0.054	2.081IlePhe: 2.081 ± 0.039	4.56IleGly: 4.56 ± 0.067	1.087IleHis: 1.087 ± 0.025	2.648IleIle: 2.648 ± 0.046	2.191IleLys: 2.191 ± 0.035	5.261IleLeu: 5.261 ± 0.071	0.827IleMet: 0.827 ± 0.023	2.108IleAsn: 2.108 ± 0.044	2.897IlePro: 2.897 ± 0.042	2.413IleGln: 2.413 ± 0.038	3.112IleArg: 3.112 ± 0.043	4.214IleSer: 4.214 ± 0.055	3.366IleThr: 3.366 ± 0.044	4.233IleVal: 4.233 ± 0.054	0.715IleTrp: 0.715 ± 0.022	1.536IleTyr: 1.536 ± 0.032	0.001IleXaa: 0.001 ± 0.001
Lys	4.131LysAla: 4.131 ± 0.055	0.24LysCys: 0.24 ± 0.012	2.093LysAsp: 2.093 ± 0.035	2.448LysGlu: 2.448 ± 0.042	1.174LysPhe: 1.174 ± 0.029	2.753LysGly: 2.753 ± 0.047	0.748LysHis: 0.748 ± 0.024	2.226LysIle: 2.226 ± 0.04	1.897LysLys: 1.897 ± 0.048	4.095LysLeu: 4.095 ± 0.053	0.775LysMet: 0.775 ± 0.021	1.263LysAsn: 1.263 ± 0.029	2.041LysPro: 2.041 ± 0.04	2.342LysGln: 2.342 ± 0.04	2.513LysArg: 2.513 ± 0.045	2.634LysSer: 2.634 ± 0.045	2.639LysThr: 2.639 ± 0.047	2.859LysVal: 2.859 ± 0.046	0.407LysTrp: 0.407 ± 0.018	0.919LysTyr: 0.919 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	9.856LeuAla: 9.856 ± 0.092	1.085LeuCys: 1.085 ± 0.028	5.713LeuAsp: 5.713 ± 0.072	6.913LeuGlu: 6.913 ± 0.073	3.76LeuPhe: 3.76 ± 0.048	7.506LeuGly: 7.506 ± 0.072	1.719LeuHis: 1.719 ± 0.039	6.102LeuIle: 6.102 ± 0.061	4.645LeuLys: 4.645 ± 0.051	11.219LeuLeu: 11.219 ± 0.121	2.281LeuMet: 2.281 ± 0.04	3.784LeuAsn: 3.784 ± 0.05	5.642LeuPro: 5.642 ± 0.064	4.635LeuGln: 4.635 ± 0.063	5.636LeuArg: 5.636 ± 0.071	9.231LeuSer: 9.231 ± 0.097	6.4LeuThr: 6.4 ± 0.064	7.172LeuVal: 7.172 ± 0.069	1.449LeuTrp: 1.449 ± 0.03	2.701LeuTyr: 2.701 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	2.092MetAla: 2.092 ± 0.038	0.138MetCys: 0.138 ± 0.008	0.882MetAsp: 0.882 ± 0.024	0.95MetGlu: 0.95 ± 0.031	0.59MetPhe: 0.59 ± 0.019	1.449MetGly: 1.449 ± 0.032	0.375MetHis: 0.375 ± 0.019	1.063MetIle: 1.063 ± 0.026	0.912MetLys: 0.912 ± 0.023	2.174MetLeu: 2.174 ± 0.036	0.534MetMet: 0.534 ± 0.017	0.723MetAsn: 0.723 ± 0.021	1.139MetPro: 1.139 ± 0.027	0.877MetGln: 0.877 ± 0.026	1.123MetArg: 1.123 ± 0.027	1.555MetSer: 1.555 ± 0.03	1.42MetThr: 1.42 ± 0.027	1.492MetVal: 1.492 ± 0.029	0.162MetTrp: 0.162 ± 0.009	0.353MetTyr: 0.353 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.903AsnAla: 2.903 ± 0.039	0.351AsnCys: 0.351 ± 0.014	1.771AsnAsp: 1.771 ± 0.051	1.699AsnGlu: 1.699 ± 0.028	1.319AsnPhe: 1.319 ± 0.03	2.567AsnGly: 2.567 ± 0.084	0.73AsnHis: 0.73 ± 0.024	1.94AsnIle: 1.94 ± 0.033	1.194AsnLys: 1.194 ± 0.027	3.953AsnLeu: 3.953 ± 0.06	0.564AsnMet: 0.564 ± 0.02	1.309AsnAsn: 1.309 ± 0.036	2.224AsnPro: 2.224 ± 0.037	2.024AsnGln: 2.024 ± 0.042	2.261AsnArg: 2.261 ± 0.041	2.404AsnSer: 2.404 ± 0.049	1.913AsnThr: 1.913 ± 0.044	2.096AsnVal: 2.096 ± 0.038	0.568AsnTrp: 0.568 ± 0.019	1.059AsnTyr: 1.059 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	3.963ProAla: 3.963 ± 0.049	0.337ProCys: 0.337 ± 0.015	3.011ProAsp: 3.011 ± 0.052	3.523ProGlu: 3.523 ± 0.054	1.838ProPhe: 1.838 ± 0.034	3.072ProGly: 3.072 ± 0.047	0.862ProHis: 0.862 ± 0.028	2.979ProIle: 2.979 ± 0.046	2.021ProLys: 2.021 ± 0.042	4.688ProLeu: 4.688 ± 0.062	0.935ProMet: 0.935 ± 0.026	1.916ProAsn: 1.916 ± 0.039	2.148ProPro: 2.148 ± 0.056	2.024ProGln: 2.024 ± 0.038	1.891ProArg: 1.891 ± 0.037	3.385ProSer: 3.385 ± 0.056	3.162ProThr: 3.162 ± 0.04	3.469ProVal: 3.469 ± 0.058	0.612ProTrp: 0.612 ± 0.02	1.348ProTyr: 1.348 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	5.051GlnAla: 5.051 ± 0.074	0.353GlnCys: 0.353 ± 0.016	1.872GlnAsp: 1.872 ± 0.035	2.322GlnGlu: 2.322 ± 0.04	1.709GlnPhe: 1.709 ± 0.034	2.799GlnGly: 2.799 ± 0.041	0.899GlnHis: 0.899 ± 0.025	3.045GlnIle: 3.045 ± 0.048	2.041GlnLys: 2.041 ± 0.04	5.763GlnLeu: 5.763 ± 0.072	1.057GlnMet: 1.057 ± 0.028	1.502GlnAsn: 1.502 ± 0.035	2.569GlnPro: 2.569 ± 0.047	3.342GlnGln: 3.342 ± 0.078	3.118GlnArg: 3.118 ± 0.053	3.55GlnSer: 3.55 ± 0.049	3.194GlnThr: 3.194 ± 0.047	3.587GlnVal: 3.587 ± 0.049	0.709GlnTrp: 0.709 ± 0.024	1.139GlnTyr: 1.139 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	4.368ArgAla: 4.368 ± 0.058	0.575ArgCys: 0.575 ± 0.02	2.793ArgAsp: 2.793 ± 0.048	3.075ArgGlu: 3.075 ± 0.049	2.379ArgPhe: 2.379 ± 0.043	3.158ArgGly: 3.158 ± 0.052	1.067ArgHis: 1.067 ± 0.028	3.328ArgIle: 3.328 ± 0.043	2.203ArgLys: 2.203 ± 0.042	6.527ArgLeu: 6.527 ± 0.072	1.225ArgMet: 1.225 ± 0.029	1.886ArgAsn: 1.886 ± 0.034	2.437ArgPro: 2.437 ± 0.043	3.297ArgGln: 3.297 ± 0.045	3.649ArgArg: 3.649 ± 0.055	4.235ArgSer: 4.235 ± 0.057	2.999ArgThr: 2.999 ± 0.043	3.48ArgVal: 3.48 ± 0.048	0.931ArgTrp: 0.931 ± 0.023	2.026ArgTyr: 2.026 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	6.437SerAla: 6.437 ± 0.075	0.62SerCys: 0.62 ± 0.02	4.038SerAsp: 4.038 ± 0.058	4.197SerGlu: 4.197 ± 0.057	2.793SerPhe: 2.793 ± 0.047	5.332SerGly: 5.332 ± 0.081	1.479SerHis: 1.479 ± 0.037	3.981SerIle: 3.981 ± 0.055	2.741SerLys: 2.741 ± 0.041	7.798SerLeu: 7.798 ± 0.079	1.467SerMet: 1.467 ± 0.032	2.548SerAsn: 2.548 ± 0.05	3.561SerPro: 3.561 ± 0.051	3.563SerGln: 3.563 ± 0.055	3.784SerArg: 3.784 ± 0.049	5.36SerSer: 5.36 ± 0.074	4.083SerThr: 4.083 ± 0.062	4.835SerVal: 4.835 ± 0.057	0.966SerTrp: 0.966 ± 0.03	1.956SerTyr: 1.956 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.691ThrAla: 5.691 ± 0.065	0.518ThrCys: 0.518 ± 0.019	3.128ThrAsp: 3.128 ± 0.048	3.405ThrGlu: 3.405 ± 0.053	2.299ThrPhe: 2.299 ± 0.046	4.319ThrGly: 4.319 ± 0.057	1.153ThrHis: 1.153 ± 0.031	3.411ThrIle: 3.411 ± 0.051	2.038ThrLys: 2.038 ± 0.037	6.8ThrLeu: 6.8 ± 0.073	0.952ThrMet: 0.952 ± 0.023	1.907ThrAsn: 1.907 ± 0.039	3.176ThrPro: 3.176 ± 0.041	2.739ThrGln: 2.739 ± 0.045	2.648ThrArg: 2.648 ± 0.046	3.62ThrSer: 3.62 ± 0.052	3.525ThrThr: 3.525 ± 0.053	4.537ThrVal: 4.537 ± 0.057	0.668ThrTrp: 0.668 ± 0.021	1.712ThrTyr: 1.712 ± 0.036	0.001ThrXaa: 0.001 ± 0.001
Val	6.606ValAla: 6.606 ± 0.07	0.75ValCys: 0.75 ± 0.023	4.021ValAsp: 4.021 ± 0.058	4.726ValGlu: 4.726 ± 0.062	2.598ValPhe: 2.598 ± 0.044	5.232ValGly: 5.232 ± 0.061	1.151ValHis: 1.151 ± 0.025	4.069ValIle: 4.069 ± 0.063	2.6ValLys: 2.6 ± 0.043	7.081ValLeu: 7.081 ± 0.07	1.529ValMet: 1.529 ± 0.036	2.308ValAsn: 2.308 ± 0.039	3.103ValPro: 3.103 ± 0.044	2.686ValGln: 2.686 ± 0.04	3.665ValArg: 3.665 ± 0.051	5.077ValSer: 5.077 ± 0.061	3.862ValThr: 3.862 ± 0.056	5.407ValVal: 5.407 ± 0.068	0.87ValTrp: 0.87 ± 0.026	1.669ValTyr: 1.669 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.016TrpAla: 1.016 ± 0.028	0.145TrpCys: 0.145 ± 0.01	0.594TrpAsp: 0.594 ± 0.02	0.749TrpGlu: 0.749 ± 0.024	0.563TrpPhe: 0.563 ± 0.021	0.907TrpGly: 0.907 ± 0.026	0.351TrpHis: 0.351 ± 0.014	0.782TrpIle: 0.782 ± 0.023	0.551TrpLys: 0.551 ± 0.02	1.878TrpLeu: 1.878 ± 0.045	0.351TrpMet: 0.351 ± 0.014	0.488TrpAsn: 0.488 ± 0.019	0.511TrpPro: 0.511 ± 0.019	1.118TrpGln: 1.118 ± 0.028	0.843TrpArg: 0.843 ± 0.028	0.83TrpSer: 0.83 ± 0.023	0.646TrpThr: 0.646 ± 0.021	0.866TrpVal: 0.866 ± 0.026	0.22TrpTrp: 0.22 ± 0.012	0.356TrpTyr: 0.356 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.275TyrAla: 2.275 ± 0.036	0.358TyrCys: 0.358 ± 0.014	1.589TyrAsp: 1.589 ± 0.039	1.796TyrGlu: 1.796 ± 0.039	1.152TyrPhe: 1.152 ± 0.028	2.193TyrGly: 2.193 ± 0.037	0.576TyrHis: 0.576 ± 0.022	1.419TyrIle: 1.419 ± 0.035	0.886TyrLys: 0.886 ± 0.027	3.216TyrLeu: 3.216 ± 0.048	0.41TyrMet: 0.41 ± 0.019	0.836TyrAsn: 0.836 ± 0.025	1.401TyrPro: 1.401 ± 0.031	1.603TyrGln: 1.603 ± 0.032	2.091TyrArg: 2.091 ± 0.037	1.872TyrSer: 1.872 ± 0.032	1.422TyrThr: 1.422 ± 0.034	1.772TyrVal: 1.772 ± 0.035	0.509TyrTrp: 0.509 ± 0.017	0.891TyrTyr: 0.891 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.061XaaXaa: 0.061 ± 0.034

Statistics based on 5475 proteins (1624882 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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