Pelistega indica
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2121 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V8G9K0|V8G9K0_9BURK Uncharacterized protein OS=Pelistega indica OX=1414851 GN=V757_02280 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 9.87 PYY4 pKa = 9.86 TLEE7 pKa = 3.97 PNRR10 pKa = 11.84 NGEE13 pKa = 4.05 GLHH16 pKa = 6.87 IGIVCGRR23 pKa = 11.84 FNEE26 pKa = 4.54 EE27 pKa = 2.96 IGYY30 pKa = 10.59 AEE32 pKa = 5.31 LNACLDD38 pKa = 3.84 EE39 pKa = 5.66 LGEE42 pKa = 4.35 LGVDD46 pKa = 3.97 EE47 pKa = 4.41 SQIMVVTVPGALEE60 pKa = 3.92 MGLALQQMALSYY72 pKa = 10.99 EE73 pKa = 4.16 FDD75 pKa = 3.39 ALIALGAVIRR85 pKa = 11.84 GEE87 pKa = 4.25 TYY89 pKa = 10.16 HH90 pKa = 7.23 FEE92 pKa = 4.34 VVSNEE97 pKa = 3.42 SAAAITRR104 pKa = 11.84 VGLDD108 pKa = 3.19 MNIPIANGILTCEE121 pKa = 4.24 TDD123 pKa = 3.08 EE124 pKa = 4.33 QAQEE128 pKa = 3.78 RR129 pKa = 11.84 AEE131 pKa = 4.36 TKK133 pKa = 10.69 GRR135 pKa = 11.84 DD136 pKa = 3.59 CAVCAVEE143 pKa = 3.94 MANLVEE149 pKa = 4.24 ALIPEE154 pKa = 4.33 SDD156 pKa = 4.35 DD157 pKa = 4.38 DD158 pKa = 5.1 EE159 pKa = 6.25 EE160 pKa = 6.27 YY161 pKa = 11.27 DD162 pKa = 5.69 DD163 pKa = 6.13 DD164 pKa = 6.79 DD165 pKa = 6.65 DD166 pKa = 6.55 YY167 pKa = 12.15 EE168 pKa = 6.38 DD169 pKa = 4.42 EE170 pKa = 5.38 DD171 pKa = 4.44 EE172 pKa = 4.26 EE173 pKa = 4.58
Molecular weight: 18.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.694
IPC_protein 3.668
Toseland 3.478
ProMoST 3.783
Dawson 3.63
Bjellqvist 3.821
Wikipedia 3.528
Rodwell 3.503
Grimsley 3.389
Solomon 3.617
Lehninger 3.579
Nozaki 3.745
DTASelect 3.897
Thurlkill 3.516
EMBOSS 3.541
Sillero 3.783
Patrickios 0.731
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.712
Protein with the highest isoelectric point:
>tr|V8G2C7|V8G2C7_9BURK GntR family transcriptional regulator OS=Pelistega indica OX=1414851 GN=V757_07555 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 9.39 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2121
0
2121
608854
29
2684
287.1
31.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.647 ± 0.066
0.921 ± 0.018
5.205 ± 0.045
5.971 ± 0.052
4.171 ± 0.036
6.853 ± 0.053
2.232 ± 0.028
6.881 ± 0.048
5.7 ± 0.045
10.238 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.568 ± 0.027
4.356 ± 0.037
4.196 ± 0.032
4.563 ± 0.047
4.578 ± 0.046
6.175 ± 0.042
5.562 ± 0.042
6.854 ± 0.047
1.219 ± 0.02
3.105 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here