Dictyocaulus viviparus (Bovine lungworm)
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14161 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D8XDV2|A0A0D8XDV2_DICVI Deoxyribonuclease-2 domain protein OS=Dictyocaulus viviparus OX=29172 GN=DICVIV_11222 PE=3 SV=1
MM1 pKa = 7.09 YY2 pKa = 10.48 LIISFLIIVLSLLTWADD19 pKa = 3.54 TLFTQSAGVKK29 pKa = 9.66 GILMCGDD36 pKa = 3.68 RR37 pKa = 11.84 PLSNTKK43 pKa = 10.1 VKK45 pKa = 10.73 LYY47 pKa = 10.68 DD48 pKa = 4.46 DD49 pKa = 5.38 DD50 pKa = 5.24 SGPDD54 pKa = 3.7 LDD56 pKa = 5.77 DD57 pKa = 4.86 LLAEE61 pKa = 4.48 GTTDD65 pKa = 4.26 SMGQFLLYY73 pKa = 10.64 GHH75 pKa = 6.64 TSEE78 pKa = 5.9 IMTIDD83 pKa = 3.5 PKK85 pKa = 11.4 LNIYY89 pKa = 9.64 HH90 pKa = 7.4 DD91 pKa = 4.81 CDD93 pKa = 4.46 DD94 pKa = 5.42 SITVDD99 pKa = 4.08 PLIISCC105 pKa = 5.1
Molecular weight: 11.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.834
IPC_protein 3.834
Toseland 3.592
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.846
Rodwell 3.656
Grimsley 3.503
Solomon 3.834
Lehninger 3.795
Nozaki 3.986
DTASelect 4.291
Thurlkill 3.681
EMBOSS 3.859
Sillero 3.961
Patrickios 0.896
IPC_peptide 3.834
IPC2_peptide 3.923
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A0D8X6D0|A0A0D8X6D0_DICVI Kinesin-like protein OS=Dictyocaulus viviparus OX=29172 GN=DICVIV_13976 PE=3 SV=1
MM1 pKa = 7.27 LRR3 pKa = 11.84 KK4 pKa = 9.77 SSLLQQRR11 pKa = 11.84 NRR13 pKa = 11.84 CQQQQQQKK21 pKa = 10.21 KK22 pKa = 9.13 NRR24 pKa = 11.84 HH25 pKa = 3.68 QQRR28 pKa = 11.84 RR29 pKa = 11.84 NRR31 pKa = 11.84 NLQQQKK37 pKa = 10.46 KK38 pKa = 10.01 SPHH41 pKa = 4.86 QQQKK45 pKa = 9.53 SQNQQRR51 pKa = 11.84 QRR53 pKa = 11.84 KK54 pKa = 8.38 NLLQQQKK61 pKa = 9.53 SQNLRR66 pKa = 11.84 QQKK69 pKa = 10.0 KK70 pKa = 10.1 SPHH73 pKa = 4.84 QQQKK77 pKa = 8.52 SQNRR81 pKa = 11.84 QLQKK85 pKa = 10.75 RR86 pKa = 11.84 SLLRR90 pKa = 11.84 LRR92 pKa = 11.84 KK93 pKa = 9.19 NRR95 pKa = 11.84 SLRR98 pKa = 11.84 RR99 pKa = 11.84 PKK101 pKa = 10.67 KK102 pKa = 10.02 NLHH105 pKa = 5.83 LQQKK109 pKa = 9.3
Molecular weight: 13.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.501
IPC2_protein 11.155
IPC_protein 12.72
Toseland 12.896
ProMoST 13.378
Dawson 12.896
Bjellqvist 12.881
Wikipedia 13.364
Rodwell 12.691
Grimsley 12.925
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.398
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.103
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14161
0
14161
4630289
27
6005
327.0
36.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.137 ± 0.02
2.26 ± 0.015
5.427 ± 0.016
6.362 ± 0.023
4.377 ± 0.015
5.291 ± 0.027
2.481 ± 0.01
6.267 ± 0.018
5.844 ± 0.023
9.194 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.641 ± 0.009
4.706 ± 0.013
4.517 ± 0.022
3.833 ± 0.015
5.956 ± 0.018
8.275 ± 0.025
5.56 ± 0.019
6.567 ± 0.016
1.106 ± 0.008
3.201 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here