Giesbergeria anulus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria;

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9LF74|A0A1H9LF74_9BURK tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase OS=Giesbergeria anulus OX=180197 GN=miaB PE=3 SV=1
MM1 pKa = 7.71SFATTQKK8 pKa = 10.25FLSVNYY14 pKa = 9.59GVTMQQALDD23 pKa = 4.0FVAASLEE30 pKa = 4.25SPQTIFNVCKK40 pKa = 9.98QAAITNQMLADD51 pKa = 4.83IISPVLPDD59 pKa = 3.65VLPSMVAQYY68 pKa = 9.51FASFGLDD75 pKa = 3.03ATQLDD80 pKa = 5.02PVTPPDD86 pKa = 3.68GGAVIAKK93 pKa = 6.75TVNVTGAGQHH103 pKa = 5.7TDD105 pKa = 2.98AAQDD109 pKa = 3.42NTSYY113 pKa = 10.57MIGLNGEE120 pKa = 4.2YY121 pKa = 10.23NYY123 pKa = 10.13TIQNFAAGDD132 pKa = 3.74KK133 pKa = 10.92LIFPAANLATVINEE147 pKa = 4.17SFTDD151 pKa = 3.98GQLVVNYY158 pKa = 10.16AFAGQMATITLVGVPDD174 pKa = 4.17SLDD177 pKa = 3.66SNIFGPTSFKK187 pKa = 11.03SAFGSDD193 pKa = 3.17ALNN196 pKa = 3.45

Molecular weight: 20.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9PU60|A0A1H9PU60_9BURK DNA helicase OS=Giesbergeria anulus OX=180197 GN=SAMN02982919_02527 PE=3 SV=1
MM1 pKa = 7.6AFFPRR6 pKa = 11.84KK7 pKa = 9.93LIAAAMLAALSLPSLAQMPAPQPGAAPAPMVAPHH41 pKa = 5.94HH42 pKa = 6.08AVRR45 pKa = 11.84KK46 pKa = 8.66KK47 pKa = 8.91ANHH50 pKa = 5.56VKK52 pKa = 9.63RR53 pKa = 11.84HH54 pKa = 4.64SARR57 pKa = 11.84HH58 pKa = 4.95HH59 pKa = 5.68RR60 pKa = 11.84AHH62 pKa = 7.02RR63 pKa = 11.84AHH65 pKa = 6.92RR66 pKa = 11.84AHH68 pKa = 6.86RR69 pKa = 11.84RR70 pKa = 11.84AHH72 pKa = 5.63RR73 pKa = 11.84AARR76 pKa = 3.69

Molecular weight: 8.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3181

0

3181

1039527

21

3029

326.8

35.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.312 ± 0.06

0.969 ± 0.015

5.001 ± 0.03

5.29 ± 0.038

3.305 ± 0.025

7.62 ± 0.042

2.494 ± 0.023

4.53 ± 0.029

3.522 ± 0.044

11.001 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.38 ± 0.019

2.842 ± 0.033

5.181 ± 0.037

5.164 ± 0.043

6.202 ± 0.037

5.619 ± 0.031

5.489 ± 0.042

7.401 ± 0.035

1.457 ± 0.023

2.221 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski