Dichanthelium oligosanthes
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 26451 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E5V8F8|A0A1E5V8F8_9POAL Uncharacterized protein OS=Dichanthelium oligosanthes OX=888268 GN=BAE44_0017539 PE=4 SV=1
MM1 pKa = 8.07 DD2 pKa = 4.75 CTHH5 pKa = 6.92 HH6 pKa = 6.37 PHH8 pKa = 6.9 PPVPSPEE15 pKa = 4.3 PSHH18 pKa = 6.86 APAPPPTAAPQEE30 pKa = 4.13 ATGDD34 pKa = 4.1 SEE36 pKa = 5.39 SDD38 pKa = 3.53 TPVSVLLTGYY48 pKa = 10.67 DD49 pKa = 3.9 DD50 pKa = 4.72 EE51 pKa = 7.71 DD52 pKa = 4.23 DD53 pKa = 5.17 AEE55 pKa = 4.44 SCSGGGDD62 pKa = 3.95 GVCWGATSAAADD74 pKa = 3.84 DD75 pKa = 4.08 SDD77 pKa = 4.75 RR78 pKa = 11.84 DD79 pKa = 3.9 DD80 pKa = 3.68 VAVEE84 pKa = 3.89 GDD86 pKa = 3.64 EE87 pKa = 5.01 DD88 pKa = 5.17 DD89 pKa = 5.99 EE90 pKa = 4.84 VDD92 pKa = 3.01 SWMAVPWWRR101 pKa = 11.84 RR102 pKa = 11.84 TVVEE106 pKa = 4.52 DD107 pKa = 3.69 AAAHH111 pKa = 5.73 SGGGSVGGAAGVHH124 pKa = 5.14 AAEE127 pKa = 4.52 SNRR130 pKa = 11.84 LFWEE134 pKa = 4.01 ACIAHH139 pKa = 7.11 GYY141 pKa = 8.68
Molecular weight: 14.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.846
IPC_protein 3.834
Toseland 3.63
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.668
Grimsley 3.541
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.19
Thurlkill 3.681
EMBOSS 3.783
Sillero 3.961
Patrickios 1.074
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|A0A1E5VR02|A0A1E5VR02_9POAL ATPase_AAA_core domain-containing protein (Fragment) OS=Dichanthelium oligosanthes OX=888268 GN=BAE44_0011438 PE=3 SV=1
MM1 pKa = 7.87 AGRR4 pKa = 11.84 PAAIHH9 pKa = 5.29 LHH11 pKa = 4.57 QRR13 pKa = 11.84 VIAGRR18 pKa = 11.84 RR19 pKa = 11.84 ARR21 pKa = 11.84 LLVNAGALRR30 pKa = 11.84 AHH32 pKa = 7.26 LSRR35 pKa = 11.84 RR36 pKa = 11.84 VRR38 pKa = 11.84 HH39 pKa = 5.54 HH40 pKa = 6.45 PRR42 pKa = 11.84 RR43 pKa = 11.84 HH44 pKa = 3.6 VHH46 pKa = 6.39 ALL48 pKa = 3.24
Molecular weight: 5.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.501
Grimsley 13.042
Solomon 13.495
Lehninger 13.393
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.237
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26451
0
26451
9714948
9
4827
367.3
40.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.719 ± 0.028
1.932 ± 0.008
5.387 ± 0.013
5.871 ± 0.014
3.719 ± 0.01
7.411 ± 0.015
2.444 ± 0.007
4.21 ± 0.012
4.722 ± 0.015
9.638 ± 0.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.396 ± 0.006
3.369 ± 0.011
5.745 ± 0.018
3.377 ± 0.01
6.329 ± 0.017
8.181 ± 0.017
4.76 ± 0.009
6.902 ± 0.012
1.305 ± 0.006
2.573 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here