Epibacterium ulvae
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3832 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G5QT75|A0A1G5QT75_9RHOB Hemoglobin/transferrin/lactoferrin receptor protein OS=Epibacterium ulvae OX=1156985 GN=SAMN04488118_105310 PE=3 SV=1
MM1 pKa = 7.29 VFFGILCGMVFGGITALTGVISFDD25 pKa = 3.41 FSIWVALASYY35 pKa = 10.52 SVVGSTAAVITTVTLYY51 pKa = 10.67 AWSSSDD57 pKa = 5.66 DD58 pKa = 3.54 DD59 pKa = 3.84 WSDD62 pKa = 3.57 YY63 pKa = 11.56 DD64 pKa = 4.36 DD65 pKa = 4.79 NSRR68 pKa = 11.84 KK69 pKa = 9.87 DD70 pKa = 3.41 DD71 pKa = 3.69 SLEE74 pKa = 3.92 LFGSHH79 pKa = 7.1 EE80 pKa = 4.18 IDD82 pKa = 3.61 DD83 pKa = 4.13 QKK85 pKa = 11.45 VGSSIAAA92 pKa = 3.53
Molecular weight: 9.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.643
IPC_protein 3.605
Toseland 3.389
ProMoST 3.834
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.643
Rodwell 3.439
Grimsley 3.3
Solomon 3.605
Lehninger 3.567
Nozaki 3.783
DTASelect 4.062
Thurlkill 3.478
EMBOSS 3.643
Sillero 3.745
Patrickios 1.875
IPC_peptide 3.592
IPC2_peptide 3.706
IPC2.peptide.svr19 3.719
Protein with the highest isoelectric point:
>tr|A0A1G5RJ14|A0A1G5RJ14_9RHOB Flagellar L-ring protein OS=Epibacterium ulvae OX=1156985 GN=flgH PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3832
0
3832
1182256
39
2141
308.5
33.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.244 ± 0.049
0.947 ± 0.014
6.012 ± 0.038
6.057 ± 0.036
3.891 ± 0.026
8.062 ± 0.041
2.161 ± 0.022
5.427 ± 0.026
3.753 ± 0.027
10.184 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.673 ± 0.019
3.037 ± 0.023
4.726 ± 0.025
3.812 ± 0.027
6.053 ± 0.037
5.693 ± 0.03
5.59 ± 0.027
7.048 ± 0.031
1.332 ± 0.017
2.298 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here