Arthrobacter phage Abidatro
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A222ZER8|A0A222ZER8_9CAUD Helix-turn-helix DNA-binding domain protein OS=Arthrobacter phage Abidatro OX=2015853 GN=36 PE=4 SV=1
MM1 pKa = 7.46 NGIQNTEE8 pKa = 3.94 VLTANLDD15 pKa = 3.72 DD16 pKa = 5.3 EE17 pKa = 4.87 IGIEE21 pKa = 5.42 ASGSWDD27 pKa = 4.2 AIADD31 pKa = 3.73 QAGNRR36 pKa = 11.84 PTLAGYY42 pKa = 7.86 VQHH45 pKa = 7.84 DD46 pKa = 3.82 DD47 pKa = 4.21 RR48 pKa = 11.84 IVLEE52 pKa = 4.18 FTNGRR57 pKa = 11.84 EE58 pKa = 3.93 LTVRR62 pKa = 11.84 QWQNQPAGQPFDD74 pKa = 3.91 GDD76 pKa = 3.52 LWDD79 pKa = 3.78 VTPLGWSS86 pKa = 3.29
Molecular weight: 9.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.298
IPC2_protein 3.859
IPC_protein 3.795
Toseland 3.592
ProMoST 3.986
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.923
Patrickios 3.757
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.856
Protein with the highest isoelectric point:
>tr|A0A222ZGN3|A0A222ZGN3_9CAUD DNA methylase OS=Arthrobacter phage Abidatro OX=2015853 GN=40 PE=3 SV=1
MM1 pKa = 7.67 SKK3 pKa = 10.35 RR4 pKa = 11.84 PQSVTLEE11 pKa = 4.21 DD12 pKa = 3.61 YY13 pKa = 11.42 ARR15 pKa = 11.84 IHH17 pKa = 6.73 RR18 pKa = 11.84 PDD20 pKa = 3.24 LAAAEE25 pKa = 4.09 AAKK28 pKa = 10.8 LLGVTPRR35 pKa = 11.84 TITRR39 pKa = 11.84 YY40 pKa = 8.92 RR41 pKa = 11.84 ARR43 pKa = 11.84 LGVSQPSHH51 pKa = 6.31 GGAVRR56 pKa = 11.84 PSPEE60 pKa = 4.0 LLAEE64 pKa = 4.18 IAARR68 pKa = 11.84 LDD70 pKa = 3.83 DD71 pKa = 4.11 GWPVKK76 pKa = 9.19 EE77 pKa = 4.14 VCEE80 pKa = 4.21 TFHH83 pKa = 6.4 VCHH86 pKa = 6.03 KK87 pKa = 9.26 TVTRR91 pKa = 11.84 HH92 pKa = 4.58 FPGRR96 pKa = 11.84 AWTRR100 pKa = 11.84 SQIGRR105 pKa = 11.84 HH106 pKa = 5.13 ARR108 pKa = 11.84 LVSSWW113 pKa = 2.96
Molecular weight: 12.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.531
IPC_protein 10.482
Toseland 10.687
ProMoST 10.452
Dawson 10.76
Bjellqvist 10.526
Wikipedia 11.008
Rodwell 10.847
Grimsley 10.818
Solomon 10.935
Lehninger 10.906
Nozaki 10.687
DTASelect 10.511
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.716
Patrickios 10.628
IPC_peptide 10.935
IPC2_peptide 9.736
IPC2.peptide.svr19 8.644
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
66
0
66
12334
37
793
186.9
20.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.686 ± 0.475
0.73 ± 0.111
5.4 ± 0.229
6.543 ± 0.323
2.716 ± 0.171
9.875 ± 0.392
1.386 ± 0.137
3.389 ± 0.23
3.494 ± 0.266
9.129 ± 0.273
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.043 ± 0.12
2.408 ± 0.187
5.91 ± 0.266
2.7 ± 0.188
7.467 ± 0.459
5.205 ± 0.221
5.311 ± 0.362
8.7 ± 0.28
2.124 ± 0.171
1.784 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here