Amino acid dipepetide frequency for Arthrobacter phage Abidatro

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.46AlaAla: 22.46 ± 1.681
0.973AlaCys: 0.973 ± 0.392
8.919AlaAsp: 8.919 ± 0.994
8.838AlaGlu: 8.838 ± 0.913
3.162AlaPhe: 3.162 ± 0.671
16.054AlaGly: 16.054 ± 1.068
2.108AlaHis: 2.108 ± 0.506
5.108AlaIle: 5.108 ± 0.583
3.73AlaLys: 3.73 ± 0.534
10.216AlaLeu: 10.216 ± 0.995
2.676AlaMet: 2.676 ± 0.388
3.0AlaAsn: 3.0 ± 0.432
6.649AlaPro: 6.649 ± 0.917
2.676AlaGln: 2.676 ± 0.429
9.892AlaArg: 9.892 ± 1.1
6.649AlaSer: 6.649 ± 1.019
6.162AlaThr: 6.162 ± 0.838
12.162AlaVal: 12.162 ± 1.178
2.919AlaTrp: 2.919 ± 0.432
2.514AlaTyr: 2.514 ± 0.355
0.0AlaXaa: 0.0 ± 0.0
Cys
0.649CysAla: 0.649 ± 0.228
0.0CysCys: 0.0 ± 0.0
0.243CysAsp: 0.243 ± 0.149
0.405CysGlu: 0.405 ± 0.159
0.0CysPhe: 0.0 ± 0.0
1.378CysGly: 1.378 ± 0.393
0.243CysHis: 0.243 ± 0.143
0.243CysIle: 0.243 ± 0.134
0.0CysLys: 0.0 ± 0.0
0.811CysLeu: 0.811 ± 0.285
0.162CysMet: 0.162 ± 0.105
0.243CysAsn: 0.243 ± 0.151
0.324CysPro: 0.324 ± 0.156
0.324CysGln: 0.324 ± 0.161
0.649CysArg: 0.649 ± 0.237
0.73CysSer: 0.73 ± 0.28
0.405CysThr: 0.405 ± 0.166
0.324CysVal: 0.324 ± 0.166
0.162CysTrp: 0.162 ± 0.124
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
6.243AspAla: 6.243 ± 0.582
0.243AspCys: 0.243 ± 0.135
2.757AspAsp: 2.757 ± 0.561
4.46AspGlu: 4.46 ± 0.562
1.946AspPhe: 1.946 ± 0.332
5.351AspGly: 5.351 ± 0.793
1.216AspHis: 1.216 ± 0.299
1.459AspIle: 1.459 ± 0.327
1.297AspLys: 1.297 ± 0.357
5.27AspLeu: 5.27 ± 0.703
1.216AspMet: 1.216 ± 0.382
0.973AspAsn: 0.973 ± 0.259
3.568AspPro: 3.568 ± 0.51
1.459AspGln: 1.459 ± 0.322
3.487AspArg: 3.487 ± 0.491
3.0AspSer: 3.0 ± 0.534
2.351AspThr: 2.351 ± 0.525
5.027AspVal: 5.027 ± 0.883
1.297AspTrp: 1.297 ± 0.276
1.378AspTyr: 1.378 ± 0.349
0.0AspXaa: 0.0 ± 0.0
Glu
7.46GluAla: 7.46 ± 0.738
0.568GluCys: 0.568 ± 0.197
2.676GluAsp: 2.676 ± 0.465
4.784GluGlu: 4.784 ± 0.766
2.108GluPhe: 2.108 ± 0.509
4.622GluGly: 4.622 ± 0.545
0.811GluHis: 0.811 ± 0.295
2.514GluIle: 2.514 ± 0.474
2.432GluLys: 2.432 ± 0.405
8.27GluLeu: 8.27 ± 0.824
1.297GluMet: 1.297 ± 0.266
1.297GluAsn: 1.297 ± 0.314
3.649GluPro: 3.649 ± 0.587
2.514GluGln: 2.514 ± 0.563
5.838GluArg: 5.838 ± 0.61
2.027GluSer: 2.027 ± 0.333
2.838GluThr: 2.838 ± 0.477
6.568GluVal: 6.568 ± 0.711
1.622GluTrp: 1.622 ± 0.436
1.459GluTyr: 1.459 ± 0.307
0.0GluXaa: 0.0 ± 0.0
Phe
2.838PheAla: 2.838 ± 0.458
0.081PheCys: 0.081 ± 0.086
1.865PheAsp: 1.865 ± 0.376
2.351PheGlu: 2.351 ± 0.533
0.73PhePhe: 0.73 ± 0.233
2.514PheGly: 2.514 ± 0.414
0.568PheHis: 0.568 ± 0.225
0.649PheIle: 0.649 ± 0.218
1.622PheLys: 1.622 ± 0.315
1.946PheLeu: 1.946 ± 0.336
0.892PheMet: 0.892 ± 0.263
0.649PheAsn: 0.649 ± 0.19
1.135PhePro: 1.135 ± 0.307
0.324PheGln: 0.324 ± 0.13
2.595PheArg: 2.595 ± 0.536
1.297PheSer: 1.297 ± 0.336
1.622PheThr: 1.622 ± 0.377
2.189PheVal: 2.189 ± 0.42
0.486PheTrp: 0.486 ± 0.198
0.811PheTyr: 0.811 ± 0.276
0.0PheXaa: 0.0 ± 0.0
Gly
12.568GlyAla: 12.568 ± 1.136
1.135GlyCys: 1.135 ± 0.262
5.757GlyAsp: 5.757 ± 0.628
5.757GlyGlu: 5.757 ± 0.66
2.351GlyPhe: 2.351 ± 0.5
8.919GlyGly: 8.919 ± 1.031
1.459GlyHis: 1.459 ± 0.31
3.649GlyIle: 3.649 ± 0.636
3.243GlyLys: 3.243 ± 0.498
8.27GlyLeu: 8.27 ± 0.922
2.108GlyMet: 2.108 ± 0.377
2.351GlyAsn: 2.351 ± 0.455
6.243GlyPro: 6.243 ± 0.587
3.487GlyGln: 3.487 ± 0.452
8.108GlyArg: 8.108 ± 0.774
5.108GlySer: 5.108 ± 0.672
4.865GlyThr: 4.865 ± 0.599
8.514GlyVal: 8.514 ± 0.805
2.676GlyTrp: 2.676 ± 0.519
2.189GlyTyr: 2.189 ± 0.413
0.0GlyXaa: 0.0 ± 0.0
His
1.216HisAla: 1.216 ± 0.273
0.081HisCys: 0.081 ± 0.078
0.973HisAsp: 0.973 ± 0.337
0.486HisGlu: 0.486 ± 0.173
0.405HisPhe: 0.405 ± 0.145
1.459HisGly: 1.459 ± 0.388
0.243HisHis: 0.243 ± 0.163
0.486HisIle: 0.486 ± 0.225
0.73HisLys: 0.73 ± 0.194
1.459HisLeu: 1.459 ± 0.338
0.162HisMet: 0.162 ± 0.114
0.162HisAsn: 0.162 ± 0.122
1.054HisPro: 1.054 ± 0.303
0.811HisGln: 0.811 ± 0.21
0.73HisArg: 0.73 ± 0.215
0.649HisSer: 0.649 ± 0.25
0.486HisThr: 0.486 ± 0.194
1.135HisVal: 1.135 ± 0.329
0.568HisTrp: 0.568 ± 0.273
0.568HisTyr: 0.568 ± 0.279
0.0HisXaa: 0.0 ± 0.0
Ile
5.027IleAla: 5.027 ± 0.698
0.243IleCys: 0.243 ± 0.133
1.784IleAsp: 1.784 ± 0.354
1.946IleGlu: 1.946 ± 0.451
1.054IlePhe: 1.054 ± 0.249
3.811IleGly: 3.811 ± 0.419
0.73IleHis: 0.73 ± 0.282
0.811IleIle: 0.811 ± 0.224
1.216IleLys: 1.216 ± 0.374
3.0IleLeu: 3.0 ± 0.516
1.216IleMet: 1.216 ± 0.318
0.405IleAsn: 0.405 ± 0.184
2.189IlePro: 2.189 ± 0.502
1.216IleGln: 1.216 ± 0.291
2.027IleArg: 2.027 ± 0.406
1.216IleSer: 1.216 ± 0.283
2.432IleThr: 2.432 ± 0.387
2.676IleVal: 2.676 ± 0.458
0.486IleTrp: 0.486 ± 0.215
0.405IleTyr: 0.405 ± 0.175
0.0IleXaa: 0.0 ± 0.0
Lys
4.784LysAla: 4.784 ± 0.736
0.081LysCys: 0.081 ± 0.081
1.946LysAsp: 1.946 ± 0.51
1.703LysGlu: 1.703 ± 0.294
1.459LysPhe: 1.459 ± 0.31
3.324LysGly: 3.324 ± 0.82
0.324LysHis: 0.324 ± 0.146
1.216LysIle: 1.216 ± 0.274
1.216LysLys: 1.216 ± 0.321
3.162LysLeu: 3.162 ± 0.457
1.216LysMet: 1.216 ± 0.315
0.892LysAsn: 0.892 ± 0.266
2.757LysPro: 2.757 ± 0.606
0.973LysGln: 0.973 ± 0.281
2.027LysArg: 2.027 ± 0.377
2.351LysSer: 2.351 ± 0.394
2.676LysThr: 2.676 ± 0.561
2.108LysVal: 2.108 ± 0.405
0.486LysTrp: 0.486 ± 0.162
0.243LysTyr: 0.243 ± 0.129
0.0LysXaa: 0.0 ± 0.0
Leu
12.649LeuAla: 12.649 ± 1.156
0.892LeuCys: 0.892 ± 0.282
5.514LeuAsp: 5.514 ± 0.84
7.541LeuGlu: 7.541 ± 0.676
1.622LeuPhe: 1.622 ± 0.405
8.676LeuGly: 8.676 ± 0.827
0.568LeuHis: 0.568 ± 0.257
3.0LeuIle: 3.0 ± 0.455
3.811LeuLys: 3.811 ± 0.802
8.27LeuLeu: 8.27 ± 0.891
1.703LeuMet: 1.703 ± 0.32
1.865LeuAsn: 1.865 ± 0.37
5.919LeuPro: 5.919 ± 0.646
2.027LeuGln: 2.027 ± 0.344
7.622LeuArg: 7.622 ± 0.813
4.378LeuSer: 4.378 ± 0.67
5.433LeuThr: 5.433 ± 0.699
7.135LeuVal: 7.135 ± 0.944
1.459LeuTrp: 1.459 ± 0.357
1.216LeuTyr: 1.216 ± 0.291
0.0LeuXaa: 0.0 ± 0.0
Met
3.081MetAla: 3.081 ± 0.438
0.0MetCys: 0.0 ± 0.0
1.135MetAsp: 1.135 ± 0.312
1.135MetGlu: 1.135 ± 0.277
0.324MetPhe: 0.324 ± 0.141
3.162MetGly: 3.162 ± 0.68
0.081MetHis: 0.081 ± 0.09
0.568MetIle: 0.568 ± 0.215
0.568MetLys: 0.568 ± 0.196
1.459MetLeu: 1.459 ± 0.32
0.162MetMet: 0.162 ± 0.113
0.324MetAsn: 0.324 ± 0.178
0.811MetPro: 0.811 ± 0.25
0.324MetGln: 0.324 ± 0.166
1.378MetArg: 1.378 ± 0.299
1.946MetSer: 1.946 ± 0.425
1.946MetThr: 1.946 ± 0.347
1.622MetVal: 1.622 ± 0.385
0.324MetTrp: 0.324 ± 0.148
0.081MetTyr: 0.081 ± 0.079
0.0MetXaa: 0.0 ± 0.0
Asn
2.595AsnAla: 2.595 ± 0.484
0.081AsnCys: 0.081 ± 0.096
1.216AsnAsp: 1.216 ± 0.286
2.108AsnGlu: 2.108 ± 0.42
0.73AsnPhe: 0.73 ± 0.3
2.838AsnGly: 2.838 ± 0.365
0.0AsnHis: 0.0 ± 0.0
0.73AsnIle: 0.73 ± 0.261
1.054AsnLys: 1.054 ± 0.34
2.757AsnLeu: 2.757 ± 0.434
0.486AsnMet: 0.486 ± 0.173
0.405AsnAsn: 0.405 ± 0.302
0.811AsnPro: 0.811 ± 0.272
0.649AsnGln: 0.649 ± 0.206
2.027AsnArg: 2.027 ± 0.441
1.378AsnSer: 1.378 ± 0.31
0.892AsnThr: 0.892 ± 0.235
2.27AsnVal: 2.27 ± 0.455
0.649AsnTrp: 0.649 ± 0.265
0.405AsnTyr: 0.405 ± 0.151
0.0AsnXaa: 0.0 ± 0.0
Pro
11.027ProAla: 11.027 ± 1.176
0.568ProCys: 0.568 ± 0.226
3.487ProAsp: 3.487 ± 0.53
4.865ProGlu: 4.865 ± 0.634
1.297ProPhe: 1.297 ± 0.366
6.649ProGly: 6.649 ± 0.804
0.649ProHis: 0.649 ± 0.243
1.541ProIle: 1.541 ± 0.359
1.784ProLys: 1.784 ± 0.401
3.73ProLeu: 3.73 ± 0.513
0.73ProMet: 0.73 ± 0.227
0.973ProAsn: 0.973 ± 0.266
2.919ProPro: 2.919 ± 0.52
1.054ProGln: 1.054 ± 0.256
3.973ProArg: 3.973 ± 0.458
4.054ProSer: 4.054 ± 0.75
2.108ProThr: 2.108 ± 0.495
5.595ProVal: 5.595 ± 0.633
1.622ProTrp: 1.622 ± 0.285
0.486ProTyr: 0.486 ± 0.214
0.0ProXaa: 0.0 ± 0.0
Gln
4.135GlnAla: 4.135 ± 0.591
0.162GlnCys: 0.162 ± 0.114
1.297GlnAsp: 1.297 ± 0.3
1.622GlnGlu: 1.622 ± 0.304
0.811GlnPhe: 0.811 ± 0.224
1.946GlnGly: 1.946 ± 0.319
0.486GlnHis: 0.486 ± 0.191
1.135GlnIle: 1.135 ± 0.294
0.811GlnLys: 0.811 ± 0.252
3.324GlnLeu: 3.324 ± 0.454
0.324GlnMet: 0.324 ± 0.159
1.216GlnAsn: 1.216 ± 0.321
1.622GlnPro: 1.622 ± 0.37
0.73GlnGln: 0.73 ± 0.274
1.378GlnArg: 1.378 ± 0.448
0.405GlnSer: 0.405 ± 0.17
1.378GlnThr: 1.378 ± 0.355
3.081GlnVal: 3.081 ± 0.503
0.649GlnTrp: 0.649 ± 0.171
0.486GlnTyr: 0.486 ± 0.196
0.0GlnXaa: 0.0 ± 0.0
Arg
9.649ArgAla: 9.649 ± 1.079
0.486ArgCys: 0.486 ± 0.202
3.973ArgAsp: 3.973 ± 0.669
3.811ArgGlu: 3.811 ± 0.486
2.919ArgPhe: 2.919 ± 0.405
7.297ArgGly: 7.297 ± 0.993
1.216ArgHis: 1.216 ± 0.345
3.081ArgIle: 3.081 ± 0.535
3.0ArgLys: 3.0 ± 0.459
7.135ArgLeu: 7.135 ± 0.702
1.541ArgMet: 1.541 ± 0.354
2.027ArgAsn: 2.027 ± 0.402
4.297ArgPro: 4.297 ± 0.68
1.865ArgGln: 1.865 ± 0.331
6.487ArgArg: 6.487 ± 0.948
3.324ArgSer: 3.324 ± 0.535
4.297ArgThr: 4.297 ± 0.592
5.433ArgVal: 5.433 ± 0.691
1.054ArgTrp: 1.054 ± 0.26
1.784ArgTyr: 1.784 ± 0.428
0.0ArgXaa: 0.0 ± 0.0
Ser
7.135SerAla: 7.135 ± 0.63
0.243SerCys: 0.243 ± 0.133
1.622SerAsp: 1.622 ± 0.375
2.514SerGlu: 2.514 ± 0.365
1.378SerPhe: 1.378 ± 0.348
6.649SerGly: 6.649 ± 0.756
1.378SerHis: 1.378 ± 0.29
2.027SerIle: 2.027 ± 0.427
2.108SerLys: 2.108 ± 0.456
4.297SerLeu: 4.297 ± 0.485
1.216SerMet: 1.216 ± 0.262
1.054SerAsn: 1.054 ± 0.286
3.081SerPro: 3.081 ± 0.564
1.135SerGln: 1.135 ± 0.338
3.811SerArg: 3.811 ± 0.657
3.324SerSer: 3.324 ± 0.51
3.081SerThr: 3.081 ± 0.415
4.297SerVal: 4.297 ± 0.597
1.216SerTrp: 1.216 ± 0.271
0.486SerTyr: 0.486 ± 0.199
0.0SerXaa: 0.0 ± 0.0
Thr
8.027ThrAla: 8.027 ± 0.915
0.081ThrCys: 0.081 ± 0.072
2.351ThrAsp: 2.351 ± 0.422
3.568ThrGlu: 3.568 ± 0.599
1.054ThrPhe: 1.054 ± 0.246
4.865ThrGly: 4.865 ± 0.705
0.486ThrHis: 0.486 ± 0.165
2.432ThrIle: 2.432 ± 0.416
2.108ThrLys: 2.108 ± 0.406
4.865ThrLeu: 4.865 ± 0.642
0.73ThrMet: 0.73 ± 0.194
2.027ThrAsn: 2.027 ± 0.466
3.568ThrPro: 3.568 ± 0.503
0.811ThrGln: 0.811 ± 0.196
2.595ThrArg: 2.595 ± 0.463
2.919ThrSer: 2.919 ± 0.427
3.0ThrThr: 3.0 ± 0.479
5.27ThrVal: 5.27 ± 0.587
0.811ThrTrp: 0.811 ± 0.253
1.541ThrTyr: 1.541 ± 0.294
0.0ThrXaa: 0.0 ± 0.0
Val
11.108ValAla: 11.108 ± 0.902
0.811ValCys: 0.811 ± 0.274
3.73ValAsp: 3.73 ± 0.578
6.0ValGlu: 6.0 ± 0.699
2.595ValPhe: 2.595 ± 0.413
6.0ValGly: 6.0 ± 1.068
0.811ValHis: 0.811 ± 0.298
2.027ValIle: 2.027 ± 0.463
3.0ValLys: 3.0 ± 0.535
9.081ValLeu: 9.081 ± 0.977
1.378ValMet: 1.378 ± 0.296
2.595ValAsn: 2.595 ± 0.471
6.324ValPro: 6.324 ± 0.778
3.487ValGln: 3.487 ± 0.521
6.649ValArg: 6.649 ± 0.954
4.054ValSer: 4.054 ± 0.624
5.108ValThr: 5.108 ± 0.641
8.595ValVal: 8.595 ± 0.869
2.027ValTrp: 2.027 ± 0.37
1.622ValTyr: 1.622 ± 0.371
0.0ValXaa: 0.0 ± 0.0
Trp
2.595TrpAla: 2.595 ± 0.415
0.162TrpCys: 0.162 ± 0.105
1.378TrpAsp: 1.378 ± 0.284
0.73TrpGlu: 0.73 ± 0.222
0.568TrpPhe: 0.568 ± 0.226
1.622TrpGly: 1.622 ± 0.345
0.405TrpHis: 0.405 ± 0.17
0.73TrpIle: 0.73 ± 0.362
0.486TrpLys: 0.486 ± 0.189
2.432TrpLeu: 2.432 ± 0.431
0.649TrpMet: 0.649 ± 0.216
0.973TrpAsn: 0.973 ± 0.261
1.135TrpPro: 1.135 ± 0.258
0.892TrpGln: 0.892 ± 0.254
1.622TrpArg: 1.622 ± 0.36
1.054TrpSer: 1.054 ± 0.275
1.216TrpThr: 1.216 ± 0.285
1.784TrpVal: 1.784 ± 0.343
0.649TrpTrp: 0.649 ± 0.198
0.162TrpTyr: 0.162 ± 0.107
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.622TyrAla: 1.622 ± 0.33
0.405TyrCys: 0.405 ± 0.161
1.378TyrAsp: 1.378 ± 0.291
0.811TyrGlu: 0.811 ± 0.254
0.649TyrPhe: 0.649 ± 0.225
1.622TyrGly: 1.622 ± 0.322
0.081TyrHis: 0.081 ± 0.08
0.486TyrIle: 0.486 ± 0.203
0.73TyrLys: 0.73 ± 0.29
1.541TyrLeu: 1.541 ± 0.352
0.486TyrMet: 0.486 ± 0.207
0.649TyrAsn: 0.649 ± 0.198
1.054TyrPro: 1.054 ± 0.303
0.243TyrGln: 0.243 ± 0.137
1.784TyrArg: 1.784 ± 0.373
2.189TyrSer: 2.189 ± 0.507
0.811TyrThr: 0.811 ± 0.272
1.216TyrVal: 1.216 ± 0.251
0.081TyrTrp: 0.081 ± 0.078
0.0TyrTyr: 0.0 ± 0.0
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (12334 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski