Rhododendron virus A
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E1B261|E1B261_9VIRU Putative coat protein OS=Rhododendron virus A OX=878260 PE=4 SV=1
MM1 pKa = 7.78 EE2 pKa = 5.6 DD3 pKa = 3.06 RR4 pKa = 11.84 RR5 pKa = 11.84 AEE7 pKa = 4.13 DD8 pKa = 4.46 RR9 pKa = 11.84 LDD11 pKa = 4.38 DD12 pKa = 4.86 DD13 pKa = 5.18 LPAGGGAPPPLRR25 pKa = 11.84 GLPPLPRR32 pKa = 11.84 AEE34 pKa = 4.14 PTDD37 pKa = 4.25 AEE39 pKa = 4.13 LQAAIDD45 pKa = 3.66 EE46 pKa = 4.61 AVAVVYY52 pKa = 9.38 EE53 pKa = 4.19 AGMPAGRR60 pKa = 11.84 FTVQRR65 pKa = 11.84 VHH67 pKa = 7.07 EE68 pKa = 4.6 IGLTVEE74 pKa = 4.45 VFVKK78 pKa = 9.63 QARR81 pKa = 11.84 AVFGGKK87 pKa = 9.29 NVDD90 pKa = 3.42 QADD93 pKa = 4.34 LIFTTGIKK101 pKa = 9.67 MGVCGSLRR109 pKa = 11.84 TMEE112 pKa = 4.42 PAGFWEE118 pKa = 4.71 VIRR121 pKa = 11.84 WARR124 pKa = 11.84 SNVGRR129 pKa = 11.84 QALEE133 pKa = 3.77 TGQKK137 pKa = 8.59 VKK139 pKa = 10.69 KK140 pKa = 10.82 VEE142 pKa = 4.21 DD143 pKa = 3.63 KK144 pKa = 11.06 RR145 pKa = 11.84 AGTQTPNEE153 pKa = 4.1 VALCQIFTMQQGIMAEE169 pKa = 4.18 EE170 pKa = 4.24 VKK172 pKa = 10.32 EE173 pKa = 4.08 ARR175 pKa = 11.84 ASTQQEE181 pKa = 3.64 IDD183 pKa = 3.19 EE184 pKa = 4.33 LTRR187 pKa = 11.84 LLRR190 pKa = 11.84 LKK192 pKa = 10.34 RR193 pKa = 11.84 AEE195 pKa = 4.0 QVKK198 pKa = 10.22 VLAEE202 pKa = 4.01 IKK204 pKa = 10.48 DD205 pKa = 3.61 KK206 pKa = 11.31 YY207 pKa = 10.69 FPANIWEE214 pKa = 4.41 EE215 pKa = 3.79 PAEE218 pKa = 4.08 AEE220 pKa = 4.08 RR221 pKa = 11.84 NARR224 pKa = 11.84 CWEE227 pKa = 4.25 VYY229 pKa = 10.07 SAALVTAGRR238 pKa = 11.84 PAPIKK243 pKa = 10.02 TEE245 pKa = 3.85 AAFKK249 pKa = 10.6 LAIDD253 pKa = 4.29 AYY255 pKa = 9.86 KK256 pKa = 11.02 NFVDD260 pKa = 4.13 TEE262 pKa = 4.18 FKK264 pKa = 10.52 TRR266 pKa = 11.84 FIRR269 pKa = 11.84 SDD271 pKa = 3.14 EE272 pKa = 4.18 HH273 pKa = 7.42 QVALRR278 pKa = 11.84 KK279 pKa = 9.17 FADD282 pKa = 3.64 EE283 pKa = 5.12 RR284 pKa = 11.84 IHH286 pKa = 6.66 FLDD289 pKa = 6.09 GIGEE293 pKa = 4.17 PKK295 pKa = 10.29 KK296 pKa = 10.81 AGTFRR301 pKa = 11.84 SLLAVLGVEE310 pKa = 4.99 PSAQTTTHH318 pKa = 5.1 TEE320 pKa = 3.69 EE321 pKa = 5.61 RR322 pKa = 11.84 NDD324 pKa = 3.55 EE325 pKa = 4.29 DD326 pKa = 5.44 DD327 pKa = 4.46 SGGEE331 pKa = 3.8 SDD333 pKa = 3.7 VARR336 pKa = 11.84 GEE338 pKa = 4.16 EE339 pKa = 4.32 PEE341 pKa = 4.34 PEE343 pKa = 4.38 AGGGTSRR350 pKa = 11.84 PGASGRR356 pKa = 11.84 GAGRR360 pKa = 11.84 GKK362 pKa = 10.52 GKK364 pKa = 10.25 RR365 pKa = 11.84 KK366 pKa = 9.41 VVPEE370 pKa = 3.84 RR371 pKa = 11.84 RR372 pKa = 11.84 SLRR375 pKa = 11.84 SSKK378 pKa = 9.29 TGRR381 pKa = 11.84 VTDD384 pKa = 3.53 VSVQGAQGDD393 pKa = 4.3 GGASRR398 pKa = 11.84 PKK400 pKa = 9.9 HH401 pKa = 5.49 RR402 pKa = 11.84 RR403 pKa = 11.84 RR404 pKa = 3.44
Molecular weight: 44.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.552
IPC2_protein 6.547
IPC_protein 6.766
Toseland 6.795
ProMoST 7.293
Dawson 7.22
Bjellqvist 7.015
Wikipedia 7.19
Rodwell 7.19
Grimsley 6.883
Solomon 7.293
Lehninger 7.293
Nozaki 7.337
DTASelect 7.454
Thurlkill 7.497
EMBOSS 7.556
Sillero 7.571
Patrickios 5.105
IPC_peptide 7.293
IPC2_peptide 6.927
IPC2.peptide.svr19 6.927
Protein with the highest isoelectric point:
>tr|E1B261|E1B261_9VIRU Putative coat protein OS=Rhododendron virus A OX=878260 PE=4 SV=1
MM1 pKa = 7.78 EE2 pKa = 5.6 DD3 pKa = 3.06 RR4 pKa = 11.84 RR5 pKa = 11.84 AEE7 pKa = 4.13 DD8 pKa = 4.46 RR9 pKa = 11.84 LDD11 pKa = 4.38 DD12 pKa = 4.86 DD13 pKa = 5.18 LPAGGGAPPPLRR25 pKa = 11.84 GLPPLPRR32 pKa = 11.84 AEE34 pKa = 4.14 PTDD37 pKa = 4.25 AEE39 pKa = 4.13 LQAAIDD45 pKa = 3.66 EE46 pKa = 4.61 AVAVVYY52 pKa = 9.38 EE53 pKa = 4.19 AGMPAGRR60 pKa = 11.84 FTVQRR65 pKa = 11.84 VHH67 pKa = 7.07 EE68 pKa = 4.6 IGLTVEE74 pKa = 4.45 VFVKK78 pKa = 9.63 QARR81 pKa = 11.84 AVFGGKK87 pKa = 9.29 NVDD90 pKa = 3.42 QADD93 pKa = 4.34 LIFTTGIKK101 pKa = 9.67 MGVCGSLRR109 pKa = 11.84 TMEE112 pKa = 4.42 PAGFWEE118 pKa = 4.71 VIRR121 pKa = 11.84 WARR124 pKa = 11.84 SNVGRR129 pKa = 11.84 QALEE133 pKa = 3.77 TGQKK137 pKa = 8.59 VKK139 pKa = 10.69 KK140 pKa = 10.82 VEE142 pKa = 4.21 DD143 pKa = 3.63 KK144 pKa = 11.06 RR145 pKa = 11.84 AGTQTPNEE153 pKa = 4.1 VALCQIFTMQQGIMAEE169 pKa = 4.18 EE170 pKa = 4.24 VKK172 pKa = 10.32 EE173 pKa = 4.08 ARR175 pKa = 11.84 ASTQQEE181 pKa = 3.64 IDD183 pKa = 3.19 EE184 pKa = 4.33 LTRR187 pKa = 11.84 LLRR190 pKa = 11.84 LKK192 pKa = 10.34 RR193 pKa = 11.84 AEE195 pKa = 4.0 QVKK198 pKa = 10.22 VLAEE202 pKa = 4.01 IKK204 pKa = 10.48 DD205 pKa = 3.61 KK206 pKa = 11.31 YY207 pKa = 10.69 FPANIWEE214 pKa = 4.41 EE215 pKa = 3.79 PAEE218 pKa = 4.08 AEE220 pKa = 4.08 RR221 pKa = 11.84 NARR224 pKa = 11.84 CWEE227 pKa = 4.25 VYY229 pKa = 10.07 SAALVTAGRR238 pKa = 11.84 PAPIKK243 pKa = 10.02 TEE245 pKa = 3.85 AAFKK249 pKa = 10.6 LAIDD253 pKa = 4.29 AYY255 pKa = 9.86 KK256 pKa = 11.02 NFVDD260 pKa = 4.13 TEE262 pKa = 4.18 FKK264 pKa = 10.52 TRR266 pKa = 11.84 FIRR269 pKa = 11.84 SDD271 pKa = 3.14 EE272 pKa = 4.18 HH273 pKa = 7.42 QVALRR278 pKa = 11.84 KK279 pKa = 9.17 FADD282 pKa = 3.64 EE283 pKa = 5.12 RR284 pKa = 11.84 IHH286 pKa = 6.66 FLDD289 pKa = 6.09 GIGEE293 pKa = 4.17 PKK295 pKa = 10.29 KK296 pKa = 10.81 AGTFRR301 pKa = 11.84 SLLAVLGVEE310 pKa = 4.99 PSAQTTTHH318 pKa = 5.1 TEE320 pKa = 3.69 EE321 pKa = 5.61 RR322 pKa = 11.84 NDD324 pKa = 3.55 EE325 pKa = 4.29 DD326 pKa = 5.44 DD327 pKa = 4.46 SGGEE331 pKa = 3.8 SDD333 pKa = 3.7 VARR336 pKa = 11.84 GEE338 pKa = 4.16 EE339 pKa = 4.32 PEE341 pKa = 4.34 PEE343 pKa = 4.38 AGGGTSRR350 pKa = 11.84 PGASGRR356 pKa = 11.84 GAGRR360 pKa = 11.84 GKK362 pKa = 10.95 GNARR366 pKa = 11.84 LSRR369 pKa = 11.84 NVEE372 pKa = 3.43 VSAQVRR378 pKa = 11.84 LGEE381 pKa = 4.01 LQTYY385 pKa = 8.15 RR386 pKa = 11.84 FRR388 pKa = 11.84 VHH390 pKa = 7.71 RR391 pKa = 11.84 EE392 pKa = 3.76 MVEE395 pKa = 3.62 HH396 pKa = 6.74 RR397 pKa = 11.84 VRR399 pKa = 11.84 SIAVARR405 pKa = 11.84 SRR407 pKa = 11.84 FEE409 pKa = 3.66 AGIRR413 pKa = 11.84 RR414 pKa = 11.84 IIGGGEE420 pKa = 3.64 MRR422 pKa = 11.84 SWYY425 pKa = 9.02 TDD427 pKa = 2.35 SRR429 pKa = 11.84 MYY431 pKa = 10.69 RR432 pKa = 11.84 GGGNSNDD439 pKa = 3.26 AMKK442 pKa = 10.46 LLSAASVVTPGKK454 pKa = 10.44 FLDD457 pKa = 3.1 QCYY460 pKa = 9.29 RR461 pKa = 11.84 VSSARR466 pKa = 11.84 KK467 pKa = 9.09 ALRR470 pKa = 11.84 LPSGLRR476 pKa = 11.84 VPDD479 pKa = 3.96 SLAEE483 pKa = 4.24 CQCKK487 pKa = 8.36 TFNDD491 pKa = 3.69 QASAGPFLRR500 pKa = 11.84 AFGIKK505 pKa = 9.53 KK506 pKa = 10.18 KK507 pKa = 10.4 YY508 pKa = 9.44 GLRR511 pKa = 11.84 RR512 pKa = 11.84 MLEE515 pKa = 3.73 EE516 pKa = 4.28 FMWGIYY522 pKa = 9.83 DD523 pKa = 3.73 RR524 pKa = 11.84 YY525 pKa = 10.54 GDD527 pKa = 3.54 GRR529 pKa = 11.84 GDD531 pKa = 3.49 EE532 pKa = 4.19 RR533 pKa = 11.84 SLPFITGRR541 pKa = 11.84 IGFRR545 pKa = 11.84 TKK547 pKa = 10.67 LLEE550 pKa = 4.26 EE551 pKa = 4.75 GEE553 pKa = 4.39 ALRR556 pKa = 11.84 KK557 pKa = 8.94 IRR559 pKa = 11.84 NGEE562 pKa = 3.83 PLGRR566 pKa = 11.84 AVMMLDD572 pKa = 3.21 ALEE575 pKa = 4.77 QISSSPLYY583 pKa = 10.49 NILSGYY589 pKa = 9.64 CGRR592 pKa = 11.84 NRR594 pKa = 11.84 NKK596 pKa = 9.97 GWCEE600 pKa = 3.55 FRR602 pKa = 11.84 NTTVRR607 pKa = 11.84 ASSDD611 pKa = 2.72 WGLLYY616 pKa = 10.9 SEE618 pKa = 4.79 VEE620 pKa = 3.84 RR621 pKa = 11.84 AKK623 pKa = 10.95 CIVEE627 pKa = 4.21 LDD629 pKa = 3.0 WSKK632 pKa = 10.98 FDD634 pKa = 3.92 RR635 pKa = 11.84 EE636 pKa = 4.15 RR637 pKa = 11.84 PSRR640 pKa = 11.84 DD641 pKa = 2.65 ILFVIKK647 pKa = 10.31 VIISCFEE654 pKa = 3.81 PRR656 pKa = 11.84 NGRR659 pKa = 11.84 EE660 pKa = 3.61 RR661 pKa = 11.84 RR662 pKa = 11.84 LLEE665 pKa = 4.13 AYY667 pKa = 10.01 EE668 pKa = 4.08 IMLEE672 pKa = 3.9 RR673 pKa = 11.84 ALVHH677 pKa = 7.07 RR678 pKa = 11.84 LLLTDD683 pKa = 3.78 AGGVLEE689 pKa = 4.47 MEE691 pKa = 4.59 GMVPSGSLWTGWLDD705 pKa = 3.23 TAMNILYY712 pKa = 9.79 IRR714 pKa = 11.84 AACLGAGIASDD725 pKa = 4.99 LYY727 pKa = 11.14 SSRR730 pKa = 11.84 CAGDD734 pKa = 4.48 DD735 pKa = 3.47 NLTLFWEE742 pKa = 4.63 DD743 pKa = 3.25 QSDD746 pKa = 4.52 GILGLVKK753 pKa = 10.19 QHH755 pKa = 6.07 LNNWFRR761 pKa = 11.84 AGIKK765 pKa = 10.55 DD766 pKa = 3.61 EE767 pKa = 5.05 DD768 pKa = 4.61 FIICRR773 pKa = 11.84 GPYY776 pKa = 9.02 YY777 pKa = 10.15 VEE779 pKa = 5.41 RR780 pKa = 11.84 YY781 pKa = 8.48 QATFPIGTDD790 pKa = 3.26 LSEE793 pKa = 4.36 GTSRR797 pKa = 11.84 LMDD800 pKa = 3.42 SAIWVRR806 pKa = 11.84 LEE808 pKa = 3.86 GGPIIDD814 pKa = 3.84 ANRR817 pKa = 11.84 GLSHH821 pKa = 6.37 RR822 pKa = 11.84 WEE824 pKa = 4.12 YY825 pKa = 11.01 RR826 pKa = 11.84 FHH828 pKa = 7.2 GKK830 pKa = 9.25 PKK832 pKa = 10.31 FLSCYY837 pKa = 8.02 WLPDD841 pKa = 3.23 GRR843 pKa = 11.84 PIRR846 pKa = 11.84 PAHH849 pKa = 6.66 DD850 pKa = 3.97 CLEE853 pKa = 4.17 KK854 pKa = 11.01 LLWPEE859 pKa = 5.03 GIHH862 pKa = 7.16 GDD864 pKa = 3.83 LEE866 pKa = 4.49 TYY868 pKa = 8.56 EE869 pKa = 4.24 AAVISMIVDD878 pKa = 3.26 NPFNHH883 pKa = 6.73 HH884 pKa = 6.14 NVNHH888 pKa = 5.58 MLVRR892 pKa = 11.84 YY893 pKa = 9.31 VLIQQIKK900 pKa = 7.99 RR901 pKa = 11.84 QVVSPMTAEE910 pKa = 4.87 DD911 pKa = 3.38 IVYY914 pKa = 9.97 LCKK917 pKa = 10.52 FRR919 pKa = 11.84 DD920 pKa = 3.69 RR921 pKa = 11.84 EE922 pKa = 4.45 GGSVPYY928 pKa = 10.42 PMIGPWRR935 pKa = 11.84 RR936 pKa = 11.84 GQKK939 pKa = 8.65 QGRR942 pKa = 11.84 MEE944 pKa = 5.31 DD945 pKa = 3.81 YY946 pKa = 10.69 PEE948 pKa = 3.94 VGRR951 pKa = 11.84 DD952 pKa = 3.03 IKK954 pKa = 10.92 NLKK957 pKa = 10.06 GFVAGISTLYY967 pKa = 9.94 ARR969 pKa = 11.84 KK970 pKa = 10.09 GGGGIDD976 pKa = 2.85 AWRR979 pKa = 11.84 FMDD982 pKa = 5.64 IIRR985 pKa = 11.84 GDD987 pKa = 3.45 ADD989 pKa = 3.81 LGQGQFGNEE998 pKa = 3.74 VLEE1001 pKa = 4.09 WARR1004 pKa = 11.84 WLGRR1008 pKa = 11.84 HH1009 pKa = 5.47 EE1010 pKa = 4.1 VTKK1013 pKa = 10.05 FLRR1016 pKa = 11.84 PIKK1019 pKa = 10.24 RR1020 pKa = 11.84 MRR1022 pKa = 11.84 AEE1024 pKa = 3.95 HH1025 pKa = 6.55 GEE1027 pKa = 4.13 VIIEE1031 pKa = 4.19 GEE1033 pKa = 4.17 GRR1035 pKa = 11.84 RR1036 pKa = 11.84 QAEE1039 pKa = 3.75 AALRR1043 pKa = 11.84 ALRR1046 pKa = 11.84 EE1047 pKa = 3.86 ILNEE1051 pKa = 3.96 GTHH1054 pKa = 6.65 RR1055 pKa = 11.84 SCLDD1059 pKa = 3.13 FSFFLSDD1066 pKa = 3.84 RR1067 pKa = 11.84 LRR1069 pKa = 11.84 ASCEE1073 pKa = 3.71 DD1074 pKa = 3.41 PRR1076 pKa = 11.84 RR1077 pKa = 3.67
Molecular weight: 121.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.901
IPC2_protein 7.322
IPC_protein 7.161
Toseland 6.883
ProMoST 7.819
Dawson 7.849
Bjellqvist 8.273
Wikipedia 7.761
Rodwell 7.863
Grimsley 6.956
Solomon 7.878
Lehninger 7.907
Nozaki 8.492
DTASelect 7.995
Thurlkill 8.053
EMBOSS 8.068
Sillero 8.375
Patrickios 4.635
IPC_peptide 7.878
IPC2_peptide 7.366
IPC2.peptide.svr19 7.305
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1481
404
1077
740.5
82.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.386 ± 1.094
1.418 ± 0.329
5.672 ± 0.01
8.845 ± 0.514
3.781 ± 0.154
9.588 ± 0.032
1.553 ± 0.153
5.064 ± 0.657
4.727 ± 0.591
7.833 ± 0.92
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.161 ± 0.329
2.498 ± 0.372
4.591 ± 0.415
3.309 ± 0.438
10.128 ± 0.231
4.794 ± 0.406
4.456 ± 0.722
6.347 ± 0.645
1.688 ± 0.34
2.161 ± 0.569
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here