Streptococcus satellite phage Javan479

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZQ03|A0A4D5ZQ03_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan479 OX=2558720 GN=JavanS479_0021 PE=4 SV=1
MM1 pKa = 7.7AVFNQFWAYY10 pKa = 7.59QTLFQPTAQDD20 pKa = 2.62IKK22 pKa = 11.07LAILGYY28 pKa = 8.48FTGTLWACEE37 pKa = 4.0VSVLEE42 pKa = 4.53DD43 pKa = 3.27VEE45 pKa = 4.18LGYY48 pKa = 10.69RR49 pKa = 11.84VIVSYY54 pKa = 9.57VSCFDD59 pKa = 3.68KK60 pKa = 10.88SDD62 pKa = 3.75RR63 pKa = 11.84LLKK66 pKa = 10.14IAQAVEE72 pKa = 3.87NSQLGG77 pKa = 3.51

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZPZ8|A0A4D5ZPZ8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan479 OX=2558720 GN=JavanS479_0011 PE=4 SV=1
MM1 pKa = 7.47TYY3 pKa = 10.91KK4 pKa = 10.67LVVSDD9 pKa = 4.29EE10 pKa = 4.29VKK12 pKa = 10.72KK13 pKa = 10.48QLKK16 pKa = 10.59KK17 pKa = 9.09MDD19 pKa = 3.44KK20 pKa = 10.62HH21 pKa = 7.03VGLMLAKK28 pKa = 10.45DD29 pKa = 3.43MKK31 pKa = 10.81KK32 pKa = 10.4RR33 pKa = 11.84LDD35 pKa = 3.56GLNNPRR41 pKa = 11.84QFGKK45 pKa = 10.72ALTGQYY51 pKa = 10.18KK52 pKa = 9.89GLWRR56 pKa = 11.84YY57 pKa = 9.01RR58 pKa = 11.84VGNYY62 pKa = 8.92RR63 pKa = 11.84VICDD67 pKa = 3.2IVDD70 pKa = 3.21NEE72 pKa = 4.36MIILALEE79 pKa = 3.99VGHH82 pKa = 6.66RR83 pKa = 11.84KK84 pKa = 9.41EE85 pKa = 3.61IYY87 pKa = 9.84KK88 pKa = 10.44RR89 pKa = 3.48

Molecular weight: 10.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

3269

52

537

130.8

15.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.506 ± 0.475

0.642 ± 0.161

6.424 ± 0.543

8.015 ± 0.708

4.283 ± 0.381

4.68 ± 0.569

2.019 ± 0.183

6.608 ± 0.311

9.391 ± 0.481

9.789 ± 0.58

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.539 ± 0.252

5.262 ± 0.489

2.355 ± 0.259

4.589 ± 0.303

4.956 ± 0.262

5.476 ± 0.367

5.751 ± 0.513

5.659 ± 0.319

1.071 ± 0.143

4.986 ± 0.332
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski