Yonghaparkia sp. Root332
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2389 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Q6R9I8|A0A0Q6R9I8_9MICO 50S ribosomal protein L18 OS=Yonghaparkia sp. Root332 OX=1736516 GN=rplR PE=3 SV=1
MM1 pKa = 7.49 RR2 pKa = 11.84 TRR4 pKa = 11.84 TRR6 pKa = 11.84 LSAVALPLVAVLALSGCVPTWLSGLTGQDD35 pKa = 2.68 TSTPTGEE42 pKa = 4.11 EE43 pKa = 3.8 VEE45 pKa = 4.58 AALEE49 pKa = 4.09 PYY51 pKa = 9.58 YY52 pKa = 10.61 RR53 pKa = 11.84 QALVWEE59 pKa = 4.49 RR60 pKa = 11.84 CGGGAQCATAIAPLDD75 pKa = 3.59 WDD77 pKa = 4.55 DD78 pKa = 5.09 PGAGDD83 pKa = 5.49 DD84 pKa = 4.4 IEE86 pKa = 4.53 LALVRR91 pKa = 11.84 YY92 pKa = 8.72 RR93 pKa = 11.84 ATDD96 pKa = 3.33 EE97 pKa = 4.27 AQGSLFVNPGGPGASGFDD115 pKa = 3.64 FVRR118 pKa = 11.84 DD119 pKa = 3.69 SVDD122 pKa = 3.73 FAVSEE127 pKa = 4.04 RR128 pKa = 11.84 LRR130 pKa = 11.84 ANFDD134 pKa = 3.73 VIGWDD139 pKa = 3.26 PRR141 pKa = 11.84 GVGRR145 pKa = 11.84 SSAVTCFTDD154 pKa = 3.73 PADD157 pKa = 3.68 MDD159 pKa = 3.75 EE160 pKa = 5.21 FIYY163 pKa = 9.98 GVPEE167 pKa = 4.3 AEE169 pKa = 4.36 PEE171 pKa = 4.18 TPEE174 pKa = 3.53 WVAEE178 pKa = 4.26 VEE180 pKa = 4.4 RR181 pKa = 11.84 SAQRR185 pKa = 11.84 FVDD188 pKa = 3.91 ACAEE192 pKa = 4.1 NTGPLLEE199 pKa = 5.3 HH200 pKa = 7.03 VDD202 pKa = 3.38 TDD204 pKa = 3.78 STVRR208 pKa = 11.84 DD209 pKa = 3.69 LDD211 pKa = 3.38 MLRR214 pKa = 11.84 AVVGDD219 pKa = 3.34 EE220 pKa = 3.46 ALNYY224 pKa = 10.0 FGYY227 pKa = 10.43 SYY229 pKa = 9.97 GTEE232 pKa = 3.36 IGARR236 pKa = 11.84 YY237 pKa = 9.8 ADD239 pKa = 3.94 RR240 pKa = 11.84 FPDD243 pKa = 3.26 RR244 pKa = 11.84 VGRR247 pKa = 11.84 LVLDD251 pKa = 4.61 GATDD255 pKa = 3.48 PTTTQFEE262 pKa = 4.64 VVLAQSIGFEE272 pKa = 4.16 AALTTYY278 pKa = 9.33 LTEE281 pKa = 4.3 CSAAASCPFPTDD293 pKa = 2.95 TTAALAIVEE302 pKa = 3.98 QLYY305 pKa = 9.39 QQLEE309 pKa = 4.16 ADD311 pKa = 5.59 PIPAADD317 pKa = 3.56 GRR319 pKa = 11.84 LFTDD323 pKa = 3.44 SVLDD327 pKa = 3.59 IAVATALYY335 pKa = 10.68 DD336 pKa = 3.6 EE337 pKa = 5.37 ASWEE341 pKa = 4.16 FLSQMFTEE349 pKa = 4.53 LRR351 pKa = 11.84 TGVVEE356 pKa = 4.3 TGFLLADD363 pKa = 4.25 FYY365 pKa = 11.6 YY366 pKa = 10.74 GRR368 pKa = 11.84 EE369 pKa = 3.75 NGEE372 pKa = 3.99 YY373 pKa = 10.41 VDD375 pKa = 4.47 NSLEE379 pKa = 3.8 AFIAINCLDD388 pKa = 3.98 YY389 pKa = 10.84 PVEE392 pKa = 4.74 RR393 pKa = 11.84 DD394 pKa = 3.14 PEE396 pKa = 4.53 TIVEE400 pKa = 4.08 QNAAIAEE407 pKa = 4.28 AAPVTSDD414 pKa = 3.27 PSILGDD420 pKa = 3.41 VVCQRR425 pKa = 11.84 WPFAFEE431 pKa = 4.08 GQLGPVSAEE440 pKa = 3.71 GAPPILVVGTTGDD453 pKa = 3.54 PATPYY458 pKa = 10.23 VWAEE462 pKa = 3.85 ALASQLDD469 pKa = 3.95 SGVLLTYY476 pKa = 10.59 DD477 pKa = 3.85 GEE479 pKa = 4.33 GHH481 pKa = 6.43 IAYY484 pKa = 10.04 DD485 pKa = 4.62 EE486 pKa = 4.59 GDD488 pKa = 3.45 PCINEE493 pKa = 4.32 LVDD496 pKa = 4.67 DD497 pKa = 4.38 YY498 pKa = 11.18 FVTGAVPARR507 pKa = 11.84 DD508 pKa = 3.84 PVCAGG513 pKa = 3.26
Molecular weight: 55.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.567
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.088
Thurlkill 3.605
EMBOSS 3.681
Sillero 3.897
Patrickios 0.973
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.812
Protein with the highest isoelectric point:
>tr|A0A0Q6RN09|A0A0Q6RN09_9MICO Malto-oligosyltrehalose trehalohydrolase OS=Yonghaparkia sp. Root332 OX=1736516 GN=ASC54_02485 PE=3 SV=1
MM1 pKa = 7.07 TRR3 pKa = 11.84 SLHH6 pKa = 5.25 FVRR9 pKa = 11.84 SARR12 pKa = 11.84 PHH14 pKa = 6.65 ALLACAGAPSRR25 pKa = 11.84 AVAPLGPRR33 pKa = 11.84 TARR36 pKa = 11.84 RR37 pKa = 11.84 VIAPRR42 pKa = 11.84 TVVARR47 pKa = 11.84 RR48 pKa = 11.84 IPVALPGAVAARR60 pKa = 11.84 GAVLLPRR67 pKa = 11.84 AVASRR72 pKa = 11.84 RR73 pKa = 11.84 IAVTARR79 pKa = 11.84 TIGAVRR85 pKa = 11.84 PCPARR90 pKa = 11.84 SIAVTLPRR98 pKa = 11.84 LIATRR103 pKa = 11.84 SLAIPLPRR111 pKa = 11.84 PVAARR116 pKa = 11.84 RR117 pKa = 11.84 AVLLPRR123 pKa = 11.84 AIAAGRR129 pKa = 11.84 VALTAGPIGAIRR141 pKa = 11.84 ASATRR146 pKa = 11.84 SLAIALPRR154 pKa = 11.84 LTATRR159 pKa = 11.84 GLAVPLPGLVATRR172 pKa = 11.84 GLAVPPGPIGAFRR185 pKa = 11.84 ASTTRR190 pKa = 11.84 SLAVPLPRR198 pKa = 11.84 AIPSGRR204 pKa = 11.84 VALPTGAA211 pKa = 4.38
Molecular weight: 21.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.501
Grimsley 13.042
Solomon 13.495
Lehninger 13.393
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.208
IPC_peptide 13.51
IPC2_peptide 12.501
IPC2.peptide.svr19 9.222
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2389
0
2389
775821
27
1968
324.7
34.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.215 ± 0.084
0.457 ± 0.012
6.114 ± 0.043
6.033 ± 0.04
2.922 ± 0.029
8.999 ± 0.045
1.943 ± 0.022
4.643 ± 0.031
1.644 ± 0.039
10.393 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.729 ± 0.019
1.698 ± 0.028
5.569 ± 0.041
2.559 ± 0.029
7.629 ± 0.051
5.551 ± 0.03
5.678 ± 0.038
9.0 ± 0.048
1.385 ± 0.022
1.838 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here