Amino acid dipepetide frequency for Phyllobacterium sp. YR620

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.674AlaAla: 14.674 ± 0.139	0.876AlaCys: 0.876 ± 0.027	6.301AlaAsp: 6.301 ± 0.075	6.899AlaGlu: 6.899 ± 0.078	4.526AlaPhe: 4.526 ± 0.061	9.795AlaGly: 9.795 ± 0.097	2.075AlaHis: 2.075 ± 0.044	7.121AlaIle: 7.121 ± 0.07	4.933AlaLys: 4.933 ± 0.07	11.917AlaLeu: 11.917 ± 0.113	3.301AlaMet: 3.301 ± 0.051	3.371AlaAsn: 3.371 ± 0.05	4.847AlaPro: 4.847 ± 0.08	3.483AlaGln: 3.483 ± 0.049	7.147AlaArg: 7.147 ± 0.076	6.506AlaSer: 6.506 ± 0.083	5.853AlaThr: 5.853 ± 0.071	8.006AlaVal: 8.006 ± 0.08	1.341AlaTrp: 1.341 ± 0.027	2.635AlaTyr: 2.635 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.827CysAla: 0.827 ± 0.025	0.093CysCys: 0.093 ± 0.008	0.449CysAsp: 0.449 ± 0.018	0.394CysGlu: 0.394 ± 0.018	0.324CysPhe: 0.324 ± 0.017	0.835CysGly: 0.835 ± 0.027	0.203CysHis: 0.203 ± 0.013	0.414CysIle: 0.414 ± 0.019	0.212CysLys: 0.212 ± 0.012	0.722CysLeu: 0.722 ± 0.024	0.163CysMet: 0.163 ± 0.011	0.185CysAsn: 0.185 ± 0.012	0.375CysPro: 0.375 ± 0.017	0.198CysGln: 0.198 ± 0.013	0.492CysArg: 0.492 ± 0.016	0.437CysSer: 0.437 ± 0.019	0.397CysThr: 0.397 ± 0.019	0.538CysVal: 0.538 ± 0.018	0.109CysTrp: 0.109 ± 0.01	0.197CysTyr: 0.197 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	6.41AspAla: 6.41 ± 0.083	0.44AspCys: 0.44 ± 0.021	3.337AspAsp: 3.337 ± 0.058	3.532AspGlu: 3.532 ± 0.062	2.241AspPhe: 2.241 ± 0.038	4.9AspGly: 4.9 ± 0.074	1.287AspHis: 1.287 ± 0.032	3.615AspIle: 3.615 ± 0.05	2.236AspLys: 2.236 ± 0.044	5.671AspLeu: 5.671 ± 0.071	1.374AspMet: 1.374 ± 0.028	1.659AspAsn: 1.659 ± 0.035	3.294AspPro: 3.294 ± 0.053	1.765AspGln: 1.765 ± 0.034	4.016AspArg: 4.016 ± 0.064	2.279AspSer: 2.279 ± 0.039	2.773AspThr: 2.773 ± 0.05	4.187AspVal: 4.187 ± 0.064	0.919AspTrp: 0.919 ± 0.029	1.588AspTyr: 1.588 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	6.998GluAla: 6.998 ± 0.076	0.341GluCys: 0.341 ± 0.018	2.767GluAsp: 2.767 ± 0.046	3.277GluGlu: 3.277 ± 0.061	1.888GluPhe: 1.888 ± 0.041	4.078GluGly: 4.078 ± 0.064	1.21GluHis: 1.21 ± 0.027	3.798GluIle: 3.798 ± 0.054	2.749GluLys: 2.749 ± 0.054	5.393GluLeu: 5.393 ± 0.065	1.508GluMet: 1.508 ± 0.033	1.875GluAsn: 1.875 ± 0.035	2.693GluPro: 2.693 ± 0.05	2.092GluGln: 2.092 ± 0.044	4.586GluArg: 4.586 ± 0.063	2.53GluSer: 2.53 ± 0.047	3.59GluThr: 3.59 ± 0.047	3.58GluVal: 3.58 ± 0.057	0.702GluTrp: 0.702 ± 0.02	1.142GluTyr: 1.142 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	4.621PheAla: 4.621 ± 0.055	0.35PheCys: 0.35 ± 0.015	2.814PheAsp: 2.814 ± 0.048	2.246PheGlu: 2.246 ± 0.039	1.578PhePhe: 1.578 ± 0.04	3.816PheGly: 3.816 ± 0.067	0.821PheHis: 0.821 ± 0.022	2.125PheIle: 2.125 ± 0.045	1.308PheLys: 1.308 ± 0.029	3.556PheLeu: 3.556 ± 0.06	0.879PheMet: 0.879 ± 0.028	1.295PheAsn: 1.295 ± 0.033	1.671PhePro: 1.671 ± 0.032	1.093PheGln: 1.093 ± 0.029	2.221PheArg: 2.221 ± 0.044	2.457PheSer: 2.457 ± 0.046	2.106PheThr: 2.106 ± 0.039	2.99PheVal: 2.99 ± 0.048	0.592PheTrp: 0.592 ± 0.022	1.068PheTyr: 1.068 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	8.165GlyAla: 8.165 ± 0.088	0.76GlyCys: 0.76 ± 0.023	4.402GlyAsp: 4.402 ± 0.074	4.631GlyGlu: 4.631 ± 0.06	3.709GlyPhe: 3.709 ± 0.058	6.905GlyGly: 6.905 ± 0.123	1.81GlyHis: 1.81 ± 0.037	5.137GlyIle: 5.137 ± 0.063	4.043GlyLys: 4.043 ± 0.072	8.39GlyLeu: 8.39 ± 0.078	2.21GlyMet: 2.21 ± 0.046	2.667GlyAsn: 2.667 ± 0.057	3.184GlyPro: 3.184 ± 0.052	2.662GlyGln: 2.662 ± 0.048	5.198GlyArg: 5.198 ± 0.062	4.937GlySer: 4.937 ± 0.06	4.763GlyThr: 4.763 ± 0.078	5.819GlyVal: 5.819 ± 0.068	1.235GlyTrp: 1.235 ± 0.033	2.408GlyTyr: 2.408 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.167HisAla: 2.167 ± 0.038	0.199HisCys: 0.199 ± 0.012	1.252HisAsp: 1.252 ± 0.034	1.088HisGlu: 1.088 ± 0.028	0.894HisPhe: 0.894 ± 0.023	1.879HisGly: 1.879 ± 0.046	0.581HisHis: 0.581 ± 0.027	1.061HisIle: 1.061 ± 0.031	0.589HisLys: 0.589 ± 0.019	2.055HisLeu: 2.055 ± 0.043	0.522HisMet: 0.522 ± 0.02	0.522HisAsn: 0.522 ± 0.022	1.26HisPro: 1.26 ± 0.032	0.542HisGln: 0.542 ± 0.019	1.291HisArg: 1.291 ± 0.03	1.025HisSer: 1.025 ± 0.025	0.83HisThr: 0.83 ± 0.026	1.545HisVal: 1.545 ± 0.033	0.316HisTrp: 0.316 ± 0.015	0.591HisTyr: 0.591 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	8.069IleAla: 8.069 ± 0.081	0.554IleCys: 0.554 ± 0.017	4.086IleAsp: 4.086 ± 0.057	3.973IleGlu: 3.973 ± 0.057	2.17IlePhe: 2.17 ± 0.044	5.405IleGly: 5.405 ± 0.077	1.027IleHis: 1.027 ± 0.026	3.238IleIle: 3.238 ± 0.065	1.952IleLys: 1.952 ± 0.037	5.161IleLeu: 5.161 ± 0.07	1.19IleMet: 1.19 ± 0.034	1.817IleAsn: 1.817 ± 0.037	2.558IlePro: 2.558 ± 0.047	1.349IleGln: 1.349 ± 0.03	3.399IleArg: 3.399 ± 0.05	3.513IleSer: 3.513 ± 0.051	3.081IleThr: 3.081 ± 0.052	4.912IleVal: 4.912 ± 0.058	0.655IleTrp: 0.655 ± 0.022	1.319IleTyr: 1.319 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	4.658LysAla: 4.658 ± 0.064	0.192LysCys: 0.192 ± 0.014	2.227LysAsp: 2.227 ± 0.041	2.059LysGlu: 2.059 ± 0.044	1.227LysPhe: 1.227 ± 0.031	3.137LysGly: 3.137 ± 0.059	0.697LysHis: 0.697 ± 0.023	2.3LysIle: 2.3 ± 0.043	1.829LysLys: 1.829 ± 0.047	4.004LysLeu: 4.004 ± 0.062	0.953LysMet: 0.953 ± 0.027	1.239LysAsn: 1.239 ± 0.035	2.553LysPro: 2.553 ± 0.044	1.363LysGln: 1.363 ± 0.032	2.788LysArg: 2.788 ± 0.049	2.514LysSer: 2.514 ± 0.048	2.327LysThr: 2.327 ± 0.044	2.8LysVal: 2.8 ± 0.054	0.459LysTrp: 0.459 ± 0.02	0.842LysTyr: 0.842 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	12.296LeuAla: 12.296 ± 0.105	0.789LeuCys: 0.789 ± 0.022	5.819LeuAsp: 5.819 ± 0.079	5.117LeuGlu: 5.117 ± 0.066	3.757LeuPhe: 3.757 ± 0.06	8.03LeuGly: 8.03 ± 0.093	1.777LeuHis: 1.777 ± 0.033	5.673LeuIle: 5.673 ± 0.07	4.205LeuLys: 4.205 ± 0.055	9.115LeuLeu: 9.115 ± 0.101	2.309LeuMet: 2.309 ± 0.042	2.903LeuAsn: 2.903 ± 0.049	5.09LeuPro: 5.09 ± 0.066	2.836LeuGln: 2.836 ± 0.048	6.044LeuArg: 6.044 ± 0.07	6.409LeuSer: 6.409 ± 0.078	5.495LeuThr: 5.495 ± 0.059	7.386LeuVal: 7.386 ± 0.082	1.069LeuTrp: 1.069 ± 0.028	2.201LeuTyr: 2.201 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.871MetAla: 2.871 ± 0.045	0.149MetCys: 0.149 ± 0.01	1.175MetAsp: 1.175 ± 0.029	1.146MetGlu: 1.146 ± 0.027	0.75MetPhe: 0.75 ± 0.025	1.727MetGly: 1.727 ± 0.033	0.505MetHis: 0.505 ± 0.02	1.598MetIle: 1.598 ± 0.037	1.094MetLys: 1.094 ± 0.032	2.5MetLeu: 2.5 ± 0.049	0.677MetMet: 0.677 ± 0.024	0.944MetAsn: 0.944 ± 0.028	1.475MetPro: 1.475 ± 0.033	0.912MetGln: 0.912 ± 0.025	1.789MetArg: 1.789 ± 0.034	1.713MetSer: 1.713 ± 0.033	1.872MetThr: 1.872 ± 0.034	1.677MetVal: 1.677 ± 0.032	0.217MetTrp: 0.217 ± 0.012	0.33MetTyr: 0.33 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.586AsnAla: 3.586 ± 0.06	0.246AsnCys: 0.246 ± 0.014	1.76AsnAsp: 1.76 ± 0.036	1.615AsnGlu: 1.615 ± 0.036	1.208AsnPhe: 1.208 ± 0.031	2.77AsnGly: 2.77 ± 0.054	0.604AsnHis: 0.604 ± 0.021	1.793AsnIle: 1.793 ± 0.033	0.97AsnLys: 0.97 ± 0.029	2.903AsnLeu: 2.903 ± 0.042	0.733AsnMet: 0.733 ± 0.023	0.98AsnAsn: 0.98 ± 0.031	2.068AsnPro: 2.068 ± 0.038	0.927AsnGln: 0.927 ± 0.027	1.995AsnArg: 1.995 ± 0.041	1.594AsnSer: 1.594 ± 0.037	1.488AsnThr: 1.488 ± 0.033	2.292AsnVal: 2.292 ± 0.043	0.545AsnTrp: 0.545 ± 0.02	0.822AsnTyr: 0.822 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	5.602ProAla: 5.602 ± 0.079	0.278ProCys: 0.278 ± 0.014	3.467ProAsp: 3.467 ± 0.047	3.488ProGlu: 3.488 ± 0.052	2.022ProPhe: 2.022 ± 0.039	4.077ProGly: 4.077 ± 0.053	1.053ProHis: 1.053 ± 0.029	2.569ProIle: 2.569 ± 0.053	1.963ProLys: 1.963 ± 0.04	4.626ProLeu: 4.626 ± 0.067	1.118ProMet: 1.118 ± 0.031	1.507ProAsn: 1.507 ± 0.035	2.055ProPro: 2.055 ± 0.051	1.696ProGln: 1.696 ± 0.037	2.473ProArg: 2.473 ± 0.042	2.806ProSer: 2.806 ± 0.047	2.405ProThr: 2.405 ± 0.042	4.125ProVal: 4.125 ± 0.057	0.609ProTrp: 0.609 ± 0.022	1.273ProTyr: 1.273 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.646GlnAla: 3.646 ± 0.057	0.182GlnCys: 0.182 ± 0.012	1.366GlnAsp: 1.366 ± 0.033	1.508GlnGlu: 1.508 ± 0.037	1.227GlnPhe: 1.227 ± 0.028	2.195GlnGly: 2.195 ± 0.039	0.637GlnHis: 0.637 ± 0.024	1.91GlnIle: 1.91 ± 0.034	1.376GlnLys: 1.376 ± 0.032	2.99GlnLeu: 2.99 ± 0.053	0.925GlnMet: 0.925 ± 0.022	1.006GlnAsn: 1.006 ± 0.026	1.642GlnPro: 1.642 ± 0.037	1.19GlnGln: 1.19 ± 0.035	2.311GlnArg: 2.311 ± 0.047	1.981GlnSer: 1.981 ± 0.04	1.753GlnThr: 1.753 ± 0.035	2.049GlnVal: 2.049 ± 0.041	0.391GlnTrp: 0.391 ± 0.016	0.703GlnTyr: 0.703 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	6.479ArgAla: 6.479 ± 0.076	0.377ArgCys: 0.377 ± 0.016	3.696ArgAsp: 3.696 ± 0.05	3.795ArgGlu: 3.795 ± 0.062	2.828ArgPhe: 2.828 ± 0.048	4.215ArgGly: 4.215 ± 0.059	1.516ArgHis: 1.516 ± 0.036	4.03ArgIle: 4.03 ± 0.055	2.594ArgLys: 2.594 ± 0.046	6.883ArgLeu: 6.883 ± 0.076	1.712ArgMet: 1.712 ± 0.038	2.184ArgAsn: 2.184 ± 0.042	3.037ArgPro: 3.037 ± 0.057	2.422ArgGln: 2.422 ± 0.039	4.558ArgArg: 4.558 ± 0.067	3.789ArgSer: 3.789 ± 0.057	3.168ArgThr: 3.168 ± 0.051	4.184ArgVal: 4.184 ± 0.059	0.827ArgTrp: 0.827 ± 0.023	1.667ArgTyr: 1.667 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	6.231SerAla: 6.231 ± 0.068	0.396SerCys: 0.396 ± 0.019	3.301SerAsp: 3.301 ± 0.055	3.111SerGlu: 3.111 ± 0.049	2.483SerPhe: 2.483 ± 0.05	5.784SerGly: 5.784 ± 0.076	1.127SerHis: 1.127 ± 0.029	3.429SerIle: 3.429 ± 0.056	2.061SerLys: 2.061 ± 0.039	5.67SerLeu: 5.67 ± 0.071	1.511SerMet: 1.511 ± 0.033	1.729SerAsn: 1.729 ± 0.038	2.773SerPro: 2.773 ± 0.046	1.669SerGln: 1.669 ± 0.035	3.49SerArg: 3.49 ± 0.047	3.198SerSer: 3.198 ± 0.054	2.927SerThr: 2.927 ± 0.043	4.31SerVal: 4.31 ± 0.065	0.75SerTrp: 0.75 ± 0.024	1.445SerTyr: 1.445 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	6.068ThrAla: 6.068 ± 0.075	0.412ThrCys: 0.412 ± 0.019	2.967ThrAsp: 2.967 ± 0.048	2.618ThrGlu: 2.618 ± 0.038	2.241ThrPhe: 2.241 ± 0.045	5.016ThrGly: 5.016 ± 0.072	1.055ThrHis: 1.055 ± 0.029	3.536ThrIle: 3.536 ± 0.053	1.859ThrLys: 1.859 ± 0.041	5.655ThrLeu: 5.655 ± 0.071	1.27ThrMet: 1.27 ± 0.028	1.583ThrAsn: 1.583 ± 0.035	3.125ThrPro: 3.125 ± 0.043	1.444ThrGln: 1.444 ± 0.034	3.08ThrArg: 3.08 ± 0.052	3.065ThrSer: 3.065 ± 0.054	3.09ThrThr: 3.09 ± 0.056	4.282ThrVal: 4.282 ± 0.06	0.651ThrTrp: 0.651 ± 0.023	1.297ThrTyr: 1.297 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	8.581ValAla: 8.581 ± 0.087	0.586ValCys: 0.586 ± 0.021	4.131ValAsp: 4.131 ± 0.058	4.42ValGlu: 4.42 ± 0.076	2.93ValPhe: 2.93 ± 0.047	5.376ValGly: 5.376 ± 0.064	1.394ValHis: 1.394 ± 0.031	4.31ValIle: 4.31 ± 0.06	2.815ValLys: 2.815 ± 0.052	7.07ValLeu: 7.07 ± 0.07	1.841ValMet: 1.841 ± 0.04	2.229ValAsn: 2.229 ± 0.044	3.706ValPro: 3.706 ± 0.057	1.961ValGln: 1.961 ± 0.034	4.42ValArg: 4.42 ± 0.047	4.494ValSer: 4.494 ± 0.06	4.437ValThr: 4.437 ± 0.063	5.705ValVal: 5.705 ± 0.078	0.858ValTrp: 0.858 ± 0.023	1.585ValTyr: 1.585 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.136TrpAla: 1.136 ± 0.03	0.112TrpCys: 0.112 ± 0.009	0.635TrpAsp: 0.635 ± 0.025	0.529TrpGlu: 0.529 ± 0.021	0.526TrpPhe: 0.526 ± 0.021	0.84TrpGly: 0.84 ± 0.024	0.316TrpHis: 0.316 ± 0.018	0.692TrpIle: 0.692 ± 0.023	0.546TrpLys: 0.546 ± 0.018	1.583TrpLeu: 1.583 ± 0.037	0.37TrpMet: 0.37 ± 0.015	0.486TrpAsn: 0.486 ± 0.018	0.637TrpPro: 0.637 ± 0.022	0.578TrpGln: 0.578 ± 0.021	0.967TrpArg: 0.967 ± 0.029	0.823TrpSer: 0.823 ± 0.026	0.753TrpThr: 0.753 ± 0.026	0.759TrpVal: 0.759 ± 0.026	0.22TrpTrp: 0.22 ± 0.012	0.341TrpTyr: 0.341 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.521TyrAla: 2.521 ± 0.04	0.235TyrCys: 0.235 ± 0.012	1.57TyrAsp: 1.57 ± 0.044	1.354TyrGlu: 1.354 ± 0.033	1.077TyrPhe: 1.077 ± 0.028	2.254TyrGly: 2.254 ± 0.041	0.513TyrHis: 0.513 ± 0.021	1.075TyrIle: 1.075 ± 0.027	0.835TyrLys: 0.835 ± 0.023	2.456TyrLeu: 2.456 ± 0.042	0.554TyrMet: 0.554 ± 0.019	0.717TyrAsn: 0.717 ± 0.023	1.171TyrPro: 1.171 ± 0.032	0.807TyrGln: 0.807 ± 0.024	1.698TyrArg: 1.698 ± 0.035	1.324TyrSer: 1.324 ± 0.032	1.208TyrThr: 1.208 ± 0.038	1.701TyrVal: 1.701 ± 0.04	0.384TyrTrp: 0.384 ± 0.018	0.697TyrTyr: 0.697 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4392 proteins (1371109 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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