Acaromyces ingoldii
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8019 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A316YF16|A0A316YF16_9BASI Cir_N domain-containing protein OS=Acaromyces ingoldii OX=215250 GN=FA10DRAFT_174389 PE=3 SV=1
MM1 pKa = 8.02 DD2 pKa = 4.47 NMINKK7 pKa = 9.47 AKK9 pKa = 10.18 EE10 pKa = 3.96 FASSDD15 pKa = 3.33 QGRR18 pKa = 11.84 DD19 pKa = 3.28 MLNKK23 pKa = 10.35 FGGGGNNNNNDD34 pKa = 3.73 NNNSGSGGFGSGGNSGSDD52 pKa = 3.37 SYY54 pKa = 12.15 GSGGNSGNDD63 pKa = 3.33 SYY65 pKa = 12.18 GSGGNSGNDD74 pKa = 3.33 SYY76 pKa = 12.18 GSGGNSGSGGGFGGSGGRR94 pKa = 11.84 NNDD97 pKa = 3.26 DD98 pKa = 3.73 SYY100 pKa = 12.15 GGGNSGSGGGFGGNDD115 pKa = 3.36 STSSGGFGGNNQGDD129 pKa = 4.47 SYY131 pKa = 11.75 SSGNQRR137 pKa = 11.84 GGSGGGQFGSSDD149 pKa = 3.42 SDD151 pKa = 3.89 SYY153 pKa = 12.12 GSGNQQGGGGGFGSGNQGGNDD174 pKa = 3.48 SYY176 pKa = 12.08 GSGNQGSGGYY186 pKa = 10.3 GGGNQDD192 pKa = 3.47 NYY194 pKa = 11.75
Molecular weight: 18.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.694
IPC_protein 3.694
Toseland 3.452
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.745
Rodwell 3.516
Grimsley 3.363
Solomon 3.706
Lehninger 3.668
Nozaki 3.859
DTASelect 4.19
Thurlkill 3.541
EMBOSS 3.732
Sillero 3.821
Patrickios 0.54
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.767
Protein with the highest isoelectric point:
>tr|A0A316YWW8|A0A316YWW8_9BASI Histone H3 OS=Acaromyces ingoldii OX=215250 GN=FA10DRAFT_237339 PE=3 SV=1
MM1 pKa = 8.11 DD2 pKa = 4.94 SALQEE7 pKa = 4.12 QIRR10 pKa = 11.84 KK11 pKa = 9.69 GKK13 pKa = 9.37 GLKK16 pKa = 8.44 KK17 pKa = 8.67 TQTNDD22 pKa = 2.77 RR23 pKa = 11.84 SKK25 pKa = 10.5 PAVAGAVGGSGGGGGGSGGSGLAKK49 pKa = 10.33 APPVPGGGGAAAAPSSGGPPALAGLFAGGMPKK81 pKa = 10.45 LKK83 pKa = 10.39 PSGGPGAPPPTVPAARR99 pKa = 11.84 PPRR102 pKa = 11.84 PPGSAAPPPPAPPAPAAPKK121 pKa = 10.13 RR122 pKa = 11.84 PGGAPPPPPPPPAPASSVATPPRR145 pKa = 11.84 TAPPPPRR152 pKa = 11.84 PPGRR156 pKa = 11.84 GPPAPTSAPPPPRR169 pKa = 11.84 PPPSLPGRR177 pKa = 11.84 APAAPPAPPPPPPPASSSPAPPARR201 pKa = 11.84 TVPQAPAPPKK211 pKa = 10.16 RR212 pKa = 11.84 PGGAAPPPPPSRR224 pKa = 11.84 PMSSSSTVGRR234 pKa = 11.84 TVPPPPPGRR243 pKa = 11.84 GSPSSPSTAGPTRR256 pKa = 11.84 SVPPPPPRR264 pKa = 11.84 ASAASSAPPPAPPRR278 pKa = 11.84 APPAPSRR285 pKa = 11.84 MPAAPPPPAPQRR297 pKa = 11.84 GPAAPPPTAPSRR309 pKa = 11.84 APAAPPPPAPSRR321 pKa = 11.84 APATPPSRR329 pKa = 11.84 TPAAPPPPPPGRR341 pKa = 11.84 APPPTAAAPPPPSAAPPAPSSRR363 pKa = 11.84 ASLQPVARR371 pKa = 11.84 ANGFGTVGNGTGNGTASSTAPSGKK395 pKa = 10.05 SVV397 pKa = 2.87
Molecular weight: 37.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.458
IPC2_protein 10.994
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.413
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.135
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.108
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8019
0
8019
4210363
49
5290
525.0
57.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.379 ± 0.03
1.049 ± 0.009
5.777 ± 0.019
6.219 ± 0.026
3.252 ± 0.016
7.648 ± 0.025
2.38 ± 0.012
3.777 ± 0.018
4.515 ± 0.025
8.832 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.993 ± 0.008
2.933 ± 0.014
5.849 ± 0.028
4.328 ± 0.025
6.784 ± 0.024
9.301 ± 0.044
5.536 ± 0.019
6.0 ± 0.022
1.263 ± 0.009
2.186 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here