Bailinhaonella thermotolerans
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7783 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3A4A903|A0A3A4A903_9ACTN FtsX-like permease family protein OS=Bailinhaonella thermotolerans OX=1070861 GN=D5H75_29370 PE=4 SV=1
MM1 pKa = 7.21 AVEE4 pKa = 5.16 LGMEE8 pKa = 4.21 SGAVLVLSWAMDD20 pKa = 3.7 GFNEE24 pKa = 4.14 GMAIEE29 pKa = 4.23 FRR31 pKa = 11.84 SPGEE35 pKa = 4.14 AGASLPGDD43 pKa = 4.47 PIDD46 pKa = 5.05 VSDD49 pKa = 3.77 HH50 pKa = 6.02 VDD52 pKa = 2.56 WGRR55 pKa = 11.84 FLGAPIVSIGIAWHH69 pKa = 6.61 IPNEE73 pKa = 4.57 GCPEE77 pKa = 4.06 MPWAYY82 pKa = 11.13 NFGFPDD88 pKa = 4.23 GSNLVIALGEE98 pKa = 4.03 AEE100 pKa = 4.48 GAGFTYY106 pKa = 10.03 MPDD109 pKa = 3.21 ALLVIFDD116 pKa = 4.41 KK117 pKa = 11.32 SLAAAYY123 pKa = 7.84 KK124 pKa = 9.6 IPASATSSCGG134 pKa = 3.26
Molecular weight: 13.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.961
IPC_protein 3.872
Toseland 3.694
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.77
Rodwell 3.719
Grimsley 3.605
Solomon 3.834
Lehninger 3.795
Nozaki 3.986
DTASelect 4.139
Thurlkill 3.745
EMBOSS 3.783
Sillero 3.999
Patrickios 1.888
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.888
Protein with the highest isoelectric point:
>tr|A0A3A4A316|A0A3A4A316_9ACTN Uncharacterized protein OS=Bailinhaonella thermotolerans OX=1070861 GN=D5H75_40245 PE=4 SV=1
MM1 pKa = 6.96 TPRR4 pKa = 11.84 RR5 pKa = 11.84 AARR8 pKa = 11.84 PPRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 PRR16 pKa = 11.84 RR17 pKa = 11.84 VTGIRR22 pKa = 11.84 FPRR25 pKa = 11.84 RR26 pKa = 11.84 AARR29 pKa = 11.84 PRR31 pKa = 11.84 PARR34 pKa = 11.84 HH35 pKa = 6.36 PPRR38 pKa = 11.84 ALRR41 pKa = 11.84 IWSGRR46 pKa = 11.84 AGRR49 pKa = 11.84 WRR51 pKa = 11.84 GSRR54 pKa = 11.84 PPWPARR60 pKa = 11.84 RR61 pKa = 11.84 SHH63 pKa = 5.62 GCVRR67 pKa = 11.84 RR68 pKa = 11.84 PRR70 pKa = 11.84 PGPRR74 pKa = 11.84 RR75 pKa = 11.84 PGPRR79 pKa = 11.84 PDD81 pKa = 3.5 PPP83 pKa = 4.72
Molecular weight: 9.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.155
IPC_protein 12.778
Toseland 12.939
ProMoST 13.437
Dawson 12.939
Bjellqvist 12.939
Wikipedia 13.422
Rodwell 12.442
Grimsley 12.983
Solomon 13.437
Lehninger 13.334
Nozaki 12.939
DTASelect 12.939
Thurlkill 12.939
EMBOSS 13.437
Sillero 12.939
Patrickios 12.149
IPC_peptide 13.437
IPC2_peptide 12.427
IPC2.peptide.svr19 9.195
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7783
0
7783
2495893
26
4029
320.7
34.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.422 ± 0.049
0.754 ± 0.007
5.599 ± 0.021
5.798 ± 0.028
2.749 ± 0.018
9.743 ± 0.033
2.166 ± 0.013
3.272 ± 0.017
1.725 ± 0.019
10.654 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.773 ± 0.011
1.543 ± 0.013
6.592 ± 0.027
2.226 ± 0.016
8.995 ± 0.033
4.534 ± 0.018
5.36 ± 0.018
8.524 ± 0.027
1.543 ± 0.013
2.03 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here