Bean golden yellow mosaic virus (isolate Puerto Rico-Japan) (BGYMV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Bean golden yellow mosaic virus

Average proteome isoelectric point is 8.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P0CK42|NSP_BGYMJ Nuclear shuttle protein OS=Bean golden yellow mosaic virus (isolate Puerto Rico-Japan) OX=222449 GN=BR1 PE=3 SV=1
MM1 pKa = 7.94PPPQRR6 pKa = 11.84FRR8 pKa = 11.84VQSKK12 pKa = 10.67NYY14 pKa = 8.0FLTYY18 pKa = 9.27PRR20 pKa = 11.84CTIPKK25 pKa = 9.85EE26 pKa = 3.98EE27 pKa = 5.24ALSQLQKK34 pKa = 10.6IHH36 pKa = 5.55TTTNKK41 pKa = 10.13KK42 pKa = 9.85FIKK45 pKa = 10.08VCEE48 pKa = 3.97EE49 pKa = 3.6RR50 pKa = 11.84HH51 pKa = 6.79DD52 pKa = 3.77NGEE55 pKa = 4.06PHH57 pKa = 6.77LHH59 pKa = 6.7ALIQFEE65 pKa = 4.82GKK67 pKa = 9.97FICTNKK73 pKa = 10.33RR74 pKa = 11.84LFDD77 pKa = 3.98LVSTTRR83 pKa = 11.84SAHH86 pKa = 4.94FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.04SSSDD99 pKa = 3.07VKK101 pKa = 10.98EE102 pKa = 4.53YY103 pKa = 10.36IDD105 pKa = 3.91KK106 pKa = 11.23DD107 pKa = 3.59GVTIEE112 pKa = 3.92WGQFQVDD119 pKa = 3.41GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQSANDD132 pKa = 3.98SYY134 pKa = 12.06AKK136 pKa = 10.56ALNADD141 pKa = 4.31SIEE144 pKa = 4.23SALTILKK151 pKa = 10.12EE152 pKa = 4.2EE153 pKa = 4.03QPKK156 pKa = 10.91DD157 pKa = 3.6YY158 pKa = 11.08VLQNHH163 pKa = 6.96NIRR166 pKa = 11.84SNLEE170 pKa = 3.8RR171 pKa = 11.84IFFKK175 pKa = 11.01VPEE178 pKa = 4.16PWVPPFPLSSFVNIPVVMQDD198 pKa = 2.6WVDD201 pKa = 3.64DD202 pKa = 3.86YY203 pKa = 11.56FGRR206 pKa = 11.84GSAARR211 pKa = 11.84PEE213 pKa = 4.12RR214 pKa = 11.84PISIIVEE221 pKa = 4.05GDD223 pKa = 3.08SRR225 pKa = 11.84TGKK228 pKa = 8.55TMWARR233 pKa = 11.84ALGPHH238 pKa = 6.63NYY240 pKa = 10.14LSGHH244 pKa = 6.9LDD246 pKa = 3.48FNSRR250 pKa = 11.84VYY252 pKa = 11.17SNSVEE257 pKa = 4.11YY258 pKa = 10.88NVIDD262 pKa = 5.45DD263 pKa = 4.49ISPNYY268 pKa = 10.3LKK270 pKa = 10.74LKK272 pKa = 9.13HH273 pKa = 5.7WKK275 pKa = 9.67EE276 pKa = 3.98LIGAQKK282 pKa = 10.57DD283 pKa = 3.42WQSNCKK289 pKa = 9.38YY290 pKa = 10.21GKK292 pKa = 9.18PVQIKK297 pKa = 10.19GGIPSIVLCNPGEE310 pKa = 4.21GSSYY314 pKa = 11.32KK315 pKa = 10.77DD316 pKa = 3.25FLNKK320 pKa = 10.0EE321 pKa = 4.29EE322 pKa = 4.93NRR324 pKa = 11.84ALHH327 pKa = 6.09NWTIHH332 pKa = 4.93NAIFVTLTAPLYY344 pKa = 10.54QSTAQDD350 pKa = 3.73CQTT353 pKa = 3.7

Molecular weight: 40.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0CK36|REN_BGYMJ Replication enhancer protein OS=Bean golden yellow mosaic virus (isolate Puerto Rico-Japan) OX=222449 GN=AC3 PE=3 SV=1
MM1 pKa = 7.98PKK3 pKa = 9.59RR4 pKa = 11.84DD5 pKa = 3.7APWRR9 pKa = 11.84HH10 pKa = 5.12MAGTSKK16 pKa = 10.53VSRR19 pKa = 11.84SGNYY23 pKa = 9.53SPSGGMGSKK32 pKa = 10.27SNKK35 pKa = 8.59ANAWVNRR42 pKa = 11.84PMYY45 pKa = 9.63RR46 pKa = 11.84KK47 pKa = 8.62PRR49 pKa = 11.84IYY51 pKa = 10.78RR52 pKa = 11.84MYY54 pKa = 10.63KK55 pKa = 10.15SPDD58 pKa = 3.3VPKK61 pKa = 10.78GCEE64 pKa = 4.39GPCKK68 pKa = 9.35VQSYY72 pKa = 6.94EE73 pKa = 3.74QRR75 pKa = 11.84HH76 pKa = 6.38DD77 pKa = 3.16ISHH80 pKa = 6.13VGKK83 pKa = 10.58VMCISDD89 pKa = 3.41ITRR92 pKa = 11.84GNGITHH98 pKa = 6.78RR99 pKa = 11.84VGKK102 pKa = 9.44RR103 pKa = 11.84FCVKK107 pKa = 9.92SVYY110 pKa = 10.16ILGKK114 pKa = 9.37IWMDD118 pKa = 3.39EE119 pKa = 3.87NIMLKK124 pKa = 9.9NHH126 pKa = 5.62TNSVIFWLVRR136 pKa = 11.84DD137 pKa = 3.82RR138 pKa = 11.84RR139 pKa = 11.84PYY141 pKa = 8.05GTPMDD146 pKa = 4.6FGQVFNMFDD155 pKa = 3.99NEE157 pKa = 4.11PSTATVKK164 pKa = 10.81NDD166 pKa = 2.84LRR168 pKa = 11.84DD169 pKa = 3.6RR170 pKa = 11.84YY171 pKa = 10.46QVMHH175 pKa = 7.19RR176 pKa = 11.84FNAKK180 pKa = 9.84VSGGQYY186 pKa = 10.81ASNEE190 pKa = 3.84QALVRR195 pKa = 11.84RR196 pKa = 11.84FWKK199 pKa = 10.4VNNHH203 pKa = 4.48VVYY206 pKa = 10.67NHH208 pKa = 5.8QEE210 pKa = 3.47AGKK213 pKa = 10.41YY214 pKa = 8.39EE215 pKa = 4.09NHH217 pKa = 6.52TEE219 pKa = 3.99NALLLYY225 pKa = 7.29MACTHH230 pKa = 7.07ASNPVYY236 pKa = 9.86ATLKK240 pKa = 9.47IRR242 pKa = 11.84IYY244 pKa = 10.59FYY246 pKa = 11.38DD247 pKa = 4.2SITNN251 pKa = 3.6

Molecular weight: 29.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1755

79

353

195.0

22.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.615 ± 0.383

2.108 ± 0.293

5.185 ± 0.495

3.875 ± 0.403

4.16 ± 0.327

4.957 ± 0.474

4.16 ± 0.322

7.009 ± 0.568

6.439 ± 0.453

6.838 ± 0.715

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.621 ± 0.628

5.698 ± 0.694

5.185 ± 0.557

4.33 ± 0.482

6.325 ± 0.578

9.117 ± 0.992

5.356 ± 0.599

6.667 ± 0.732

1.425 ± 0.182

3.932 ± 0.528
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski