Prevotella sp. P5-92
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2803 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A255SPL9|A0A255SPL9_9BACT Uncharacterized protein OS=Prevotella sp. P5-92 OX=2024222 GN=CIK99_12900 PE=4 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.4 KK3 pKa = 10.19 FVIHH7 pKa = 5.41 YY8 pKa = 9.38 NYY10 pKa = 10.47 YY11 pKa = 8.41 ATADD15 pKa = 3.62 VTVLANSKK23 pKa = 10.54 EE24 pKa = 4.11 EE25 pKa = 4.6 AIEE28 pKa = 4.03 KK29 pKa = 10.11 ADD31 pKa = 4.25 QIEE34 pKa = 4.22 IPNDD38 pKa = 3.62 EE39 pKa = 4.82 FDD41 pKa = 4.41 LEE43 pKa = 4.26 YY44 pKa = 11.08 DD45 pKa = 3.24 NRR47 pKa = 11.84 EE48 pKa = 3.92 AFEE51 pKa = 5.02 LEE53 pKa = 4.52 DD54 pKa = 3.58 VPEE57 pKa = 4.26 LQEE60 pKa = 4.76 VIDD63 pKa = 4.25 KK64 pKa = 9.1 ATAIIKK70 pKa = 9.95 KK71 pKa = 8.99 FNEE74 pKa = 4.2 GAGQEE79 pKa = 4.32 DD80 pKa = 5.94 FYY82 pKa = 11.76 SVPCYY87 pKa = 8.72 PTVTTYY93 pKa = 10.56 CWNGDD98 pKa = 3.33 EE99 pKa = 4.48 MVKK102 pKa = 10.68 NKK104 pKa = 10.25 NAVEE108 pKa = 3.87 DD109 pKa = 4.46 FYY111 pKa = 11.86 YY112 pKa = 10.91 DD113 pKa = 3.51 SDD115 pKa = 3.92 KK116 pKa = 11.56 GLMMDD121 pKa = 3.33 VGEE124 pKa = 4.31 GFEE127 pKa = 4.22 VEE129 pKa = 3.91 ISEE132 pKa = 5.01 LSDD135 pKa = 3.52 VEE137 pKa = 4.13 QLNVCQVIINSAQANGIEE155 pKa = 4.12 LL156 pKa = 3.81
Molecular weight: 17.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.776
IPC2_protein 3.935
IPC_protein 3.897
Toseland 3.706
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.745
Rodwell 3.719
Grimsley 3.617
Solomon 3.846
Lehninger 3.795
Nozaki 3.973
DTASelect 4.126
Thurlkill 3.732
EMBOSS 3.757
Sillero 3.999
Patrickios 1.875
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.911
Protein with the highest isoelectric point:
>tr|A0A255T3A8|A0A255T3A8_9BACT Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase OS=Prevotella sp. P5-92 OX=2024222 GN=cobT PE=3 SV=1
MM1 pKa = 7.46 SIAVEE6 pKa = 4.47 GWSEE10 pKa = 4.08 TEE12 pKa = 3.65 QQYY15 pKa = 11.21 EE16 pKa = 4.19 EE17 pKa = 5.35 LGDD20 pKa = 4.03 ANTMKK25 pKa = 10.51 RR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 THH30 pKa = 6.5 KK31 pKa = 9.81 EE32 pKa = 3.13 GNMRR36 pKa = 11.84 KK37 pKa = 8.3 AHH39 pKa = 5.98 LGIRR43 pKa = 11.84 MSRR46 pKa = 11.84 VNAMRR51 pKa = 11.84 FLQRR55 pKa = 11.84 FTEE58 pKa = 4.16 AKK60 pKa = 9.52 EE61 pKa = 3.89 RR62 pKa = 11.84 SNSARR67 pKa = 11.84 RR68 pKa = 11.84 KK69 pKa = 9.57 RR70 pKa = 11.84 RR71 pKa = 11.84 TDD73 pKa = 3.24 PAVNEE78 pKa = 4.14 QPSGSTYY85 pKa = 11.23 GMM87 pKa = 4.17
Molecular weight: 10.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.604
IPC_protein 10.511
Toseland 10.774
ProMoST 10.526
Dawson 10.847
Bjellqvist 10.57
Wikipedia 11.082
Rodwell 10.979
Grimsley 10.891
Solomon 11.023
Lehninger 10.979
Nozaki 10.745
DTASelect 10.57
Thurlkill 10.76
EMBOSS 11.184
Sillero 10.789
Patrickios 10.76
IPC_peptide 11.023
IPC2_peptide 9.37
IPC2.peptide.svr19 8.765
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2803
0
2803
1003906
31
4508
358.2
40.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.351 ± 0.045
1.447 ± 0.02
6.103 ± 0.033
6.284 ± 0.045
4.297 ± 0.033
6.835 ± 0.045
1.953 ± 0.022
6.746 ± 0.039
6.53 ± 0.039
8.456 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.026 ± 0.022
5.101 ± 0.044
3.566 ± 0.025
3.302 ± 0.026
4.831 ± 0.04
6.091 ± 0.045
5.882 ± 0.039
6.674 ± 0.034
1.265 ± 0.019
4.258 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here