Motiliproteus coralliicola
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3762 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A369WLB1|A0A369WLB1_9GAMM NCS2 family permease OS=Motiliproteus coralliicola OX=2283196 GN=DV711_09930 PE=3 SV=1
MM1 pKa = 7.55 RR2 pKa = 11.84 VINTGRR8 pKa = 11.84 SNRR11 pKa = 11.84 LLTVILQLGLLMLLAASAQAGRR33 pKa = 11.84 TINSATVNGGSSATVAPGASITVTLNVTTDD63 pKa = 3.61 GNGSNNDD70 pKa = 3.4 WEE72 pKa = 4.59 STDD75 pKa = 3.24 WLIGTSSSGSYY86 pKa = 8.5 TCNNHH91 pKa = 6.75 ADD93 pKa = 3.48 NTFTGTHH100 pKa = 5.68 TEE102 pKa = 4.1 SFTITAPSTPGTYY115 pKa = 9.82 NAYY118 pKa = 9.93 FWASHH123 pKa = 6.17 NDD125 pKa = 3.1 SCGGSGSNLFTLTNAITVSTGTTCSAEE152 pKa = 4.17 TVADD156 pKa = 3.76 NFDD159 pKa = 3.78 SVSFSQNDD167 pKa = 3.7 GSRR170 pKa = 11.84 NWSGDD175 pKa = 2.74 WQEE178 pKa = 4.35 IGEE181 pKa = 4.41 SDD183 pKa = 3.7 GASAGFVRR191 pKa = 11.84 VRR193 pKa = 11.84 NDD195 pKa = 2.69 SCSSGNCLRR204 pKa = 11.84 LGVISSEE211 pKa = 4.07 PAQSFSNVGASRR223 pKa = 11.84 EE224 pKa = 3.93 VDD226 pKa = 3.28 LSGATTATLTFNYY239 pKa = 7.74 RR240 pKa = 11.84 TGVIQNSEE248 pKa = 4.4 TVSLQVSANGGSSWTTLRR266 pKa = 11.84 TYY268 pKa = 9.23 TVSSTNSSATAEE280 pKa = 4.29 SVDD283 pKa = 3.72 LSSYY287 pKa = 9.92 IASNTQIRR295 pKa = 11.84 FLASGSNAIVGFYY308 pKa = 10.71 ADD310 pKa = 5.54 DD311 pKa = 3.78 IEE313 pKa = 5.3 ISYY316 pKa = 10.28 DD317 pKa = 3.41 PCSTLLAEE325 pKa = 4.27 WRR327 pKa = 11.84 LDD329 pKa = 3.43 EE330 pKa = 4.35 TSWNGTSDD338 pKa = 3.54 EE339 pKa = 4.45 VVDD342 pKa = 5.03 SEE344 pKa = 4.63 GSNHH348 pKa = 5.65 GVANSADD355 pKa = 3.78 TASGLLCNAADD366 pKa = 4.53 LSAAGTSDD374 pKa = 4.09 YY375 pKa = 11.17 ISLGSGALNGATDD388 pKa = 3.97 FTISAWIKK396 pKa = 8.44 TSNSGSQALISGANSSQANEE416 pKa = 4.3 LIMWFPSATSFEE428 pKa = 4.28 PWLKK432 pKa = 10.78 GVDD435 pKa = 3.7 PSSISIGNIADD446 pKa = 4.07 NQWHH450 pKa = 6.23 HH451 pKa = 6.49 LVWTRR456 pKa = 11.84 SGSQNCIYY464 pKa = 10.44 RR465 pKa = 11.84 DD466 pKa = 3.62 ASLQGCTSGSTSALNIDD483 pKa = 3.72 SGGLILGQEE492 pKa = 4.09 QDD494 pKa = 3.65 SVGGGFDD501 pKa = 5.36 LGQDD505 pKa = 2.8 WRR507 pKa = 11.84 GLVDD511 pKa = 3.9 EE512 pKa = 5.24 LLIFNEE518 pKa = 4.53 AVSASQITSIYY529 pKa = 10.26 NNTLAGDD536 pKa = 3.53 NWDD539 pKa = 3.56 GTEE542 pKa = 4.0 RR543 pKa = 11.84 DD544 pKa = 4.59 CPSGSCSVTYY554 pKa = 10.94 SDD556 pKa = 5.35 DD557 pKa = 3.32 FSAASFTNSSGSVNWSGGWTEE578 pKa = 5.21 HH579 pKa = 6.61 DD580 pKa = 3.7 TASTGAAAGYY590 pKa = 10.25 VSISGGKK597 pKa = 8.97 LHH599 pKa = 6.55 LQNYY603 pKa = 6.65 TPSEE607 pKa = 4.16 SSSRR611 pKa = 11.84 PGVARR616 pKa = 11.84 GFSLADD622 pKa = 3.25 ASEE625 pKa = 4.39 AVVSYY630 pKa = 10.63 SYY632 pKa = 11.63 SLTDD636 pKa = 4.79 AVDD639 pKa = 3.54 SDD641 pKa = 4.47 DD642 pKa = 3.95 SVLVEE647 pKa = 4.24 ISDD650 pKa = 4.61 DD651 pKa = 4.28 GGSSWTTIDD660 pKa = 3.93 TITGLGGGSHH670 pKa = 6.68 SNSISLSSFSSISFTNNMQISIRR693 pKa = 11.84 ISGNINSGSCCYY705 pKa = 10.47 GGSNEE710 pKa = 4.19 IISFEE715 pKa = 4.14 FVTITTQEE723 pKa = 3.98 PCSASDD729 pKa = 3.32 VDD731 pKa = 4.92 HH732 pKa = 6.43 YY733 pKa = 11.42 AISHH737 pKa = 6.18 SGQGVTCEE745 pKa = 4.56 AEE747 pKa = 4.46 SITITAHH754 pKa = 6.62 DD755 pKa = 3.98 SSEE758 pKa = 4.12 GTVEE762 pKa = 4.11 PGNSVSLTLNTGTGIGDD779 pKa = 3.37 WSLITGNGSLANGTAGDD796 pKa = 5.06 GIASYY801 pKa = 10.63 SWGSGEE807 pKa = 4.24 SAVVLALAHH816 pKa = 6.28 PAVTTAPHH824 pKa = 6.14 MDD826 pKa = 3.27 IDD828 pKa = 4.77 LVDD831 pKa = 4.05 NNSATDD837 pKa = 3.53 KK838 pKa = 11.41 DD839 pKa = 3.96 GDD841 pKa = 3.87 GTEE844 pKa = 4.11 DD845 pKa = 3.41 PKK847 pKa = 11.32 LAFSDD852 pKa = 3.31 AGFRR856 pKa = 11.84 FYY858 pKa = 11.39 GAGVANNIGNQIAGKK873 pKa = 9.54 EE874 pKa = 4.26 SNLAPGNQTIEE885 pKa = 3.71 IRR887 pKa = 11.84 AVQTNTDD894 pKa = 3.22 TGACEE899 pKa = 4.04 ARR901 pKa = 11.84 LTNTQIVQMAYY912 pKa = 8.73 QCRR915 pKa = 11.84 NPATCQGDD923 pKa = 3.56 NKK925 pKa = 9.94 LTLNDD930 pKa = 3.63 STTLRR935 pKa = 11.84 ANPATGVTNYY945 pKa = 10.9 SNVSLNFGSNGQASFTFDD963 pKa = 3.27 YY964 pKa = 11.04 SDD966 pKa = 3.72 AGLIQLHH973 pKa = 6.21 ASKK976 pKa = 9.33 TLAASGEE983 pKa = 4.26 DD984 pKa = 3.6 PQVTLSGSSNEE995 pKa = 3.9 FVVRR999 pKa = 11.84 PFGFHH1004 pKa = 6.54 IDD1006 pKa = 3.71 VPGNPAAISAAGDD1019 pKa = 3.55 PFKK1022 pKa = 11.05 AAGEE1026 pKa = 4.09 AFAATVTAVLWASGDD1041 pKa = 3.84 DD1042 pKa = 3.81 SDD1044 pKa = 5.94 SDD1046 pKa = 4.14 GVPDD1050 pKa = 4.68 SGADD1054 pKa = 3.42 LSDD1057 pKa = 3.63 NGVTANFGQEE1067 pKa = 3.94 STAEE1071 pKa = 4.36 TVTLTQGLVLPTNGTNPALSNGTFSGFSAGASTKK1105 pKa = 10.96 SNLSWSEE1112 pKa = 3.61 VGIISLTANVTDD1124 pKa = 4.65 GSYY1127 pKa = 10.75 LGAGAVTGTLPYY1139 pKa = 10.15 VGRR1142 pKa = 11.84 FYY1144 pKa = 10.59 PSHH1147 pKa = 6.48 FRR1149 pKa = 11.84 LSAATLTNRR1158 pKa = 11.84 TDD1160 pKa = 3.54 LASCSSSSFTYY1171 pKa = 9.04 MGEE1174 pKa = 4.06 PFGVQYY1180 pKa = 10.96 SLSAEE1185 pKa = 4.06 NSSGVITRR1193 pKa = 11.84 NYY1195 pKa = 9.89 AGNFAKK1201 pKa = 10.6 LDD1203 pKa = 3.62 TSGEE1207 pKa = 4.21 LNLGAVDD1214 pKa = 4.01 GTTDD1218 pKa = 3.5 LSSRR1222 pKa = 11.84 LTLDD1226 pKa = 2.92 SASIVWPALDD1236 pKa = 3.4 HH1237 pKa = 6.46 TGAGTAAIDD1246 pKa = 3.5 LNLRR1250 pKa = 11.84 IDD1252 pKa = 4.09 RR1253 pKa = 11.84 DD1254 pKa = 4.06 SLPDD1258 pKa = 3.58 GPFAALSVGTAPNDD1272 pKa = 3.04 EE1273 pKa = 4.26 DD1274 pKa = 4.0 AQLNSFDD1281 pKa = 5.49 LDD1283 pKa = 3.7 VSGDD1287 pKa = 3.58 TFFDD1291 pKa = 3.53 HH1292 pKa = 7.11 GLLGSTRR1299 pKa = 11.84 IRR1301 pKa = 11.84 YY1302 pKa = 8.32 GRR1304 pKa = 11.84 LLVGNTFGPEE1314 pKa = 4.01 TEE1316 pKa = 4.34 DD1317 pKa = 5.02 LEE1319 pKa = 5.13 VPLQLEE1325 pKa = 4.44 YY1326 pKa = 10.87 FDD1328 pKa = 5.4 GINFILNTDD1337 pKa = 4.26 DD1338 pKa = 4.9 SCTTYY1343 pKa = 10.98 INTSTSLDD1351 pKa = 3.37 HH1352 pKa = 7.34 SNYY1355 pKa = 10.38 GSDD1358 pKa = 3.31 NLEE1361 pKa = 4.4 AGDD1364 pKa = 4.16 TSVTSPNTEE1373 pKa = 4.01 TEE1375 pKa = 4.48 VVAGQSPSSAPLVLSAPGVGNEE1397 pKa = 4.12 GQVDD1401 pKa = 5.52 LIYY1404 pKa = 10.6 AAPSWLEE1411 pKa = 3.58 FDD1413 pKa = 3.53 WFGGGDD1419 pKa = 3.58 TDD1421 pKa = 4.84 PRR1423 pKa = 11.84 GQATFGRR1430 pKa = 11.84 YY1431 pKa = 9.06 RR1432 pKa = 11.84 GNDD1435 pKa = 2.79 RR1436 pKa = 11.84 MIFWQEE1442 pKa = 3.56 NFSQQ1446 pKa = 3.6
Molecular weight: 150.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.923
IPC_protein 3.961
Toseland 3.732
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.872
Rodwell 3.783
Grimsley 3.643
Solomon 3.948
Lehninger 3.897
Nozaki 4.05
DTASelect 4.317
Thurlkill 3.783
EMBOSS 3.884
Sillero 4.075
Patrickios 1.341
IPC_peptide 3.948
IPC2_peptide 4.062
IPC2.peptide.svr19 3.955
Protein with the highest isoelectric point:
>tr|A0A369WA03|A0A369WA03_9GAMM Cupin domain-containing protein OS=Motiliproteus coralliicola OX=2283196 GN=DV711_14570 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 8.98 RR4 pKa = 11.84 TFQPSVLKK12 pKa = 10.46 RR13 pKa = 11.84 ARR15 pKa = 11.84 NHH17 pKa = 5.26 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.21 SGRR29 pKa = 11.84 QVLTRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.12 GRR40 pKa = 11.84 KK41 pKa = 8.87 SLSAA45 pKa = 3.86
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3762
0
3762
1227948
23
3900
326.4
36.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.433 ± 0.042
1.09 ± 0.016
5.729 ± 0.035
6.335 ± 0.04
3.636 ± 0.027
7.434 ± 0.034
2.191 ± 0.017
5.452 ± 0.035
3.834 ± 0.033
11.507 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.437 ± 0.02
3.333 ± 0.024
4.566 ± 0.027
5.443 ± 0.045
5.948 ± 0.036
6.284 ± 0.031
4.701 ± 0.032
6.675 ± 0.034
1.28 ± 0.015
2.692 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here