Myxococcales bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Myxococcales; unclassified Myxococcales

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7151 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q5X5Z9|A0A4Q5X5Z9_9DELT Antibiotic biosynthesis monooxygenase OS=Myxococcales bacterium OX=2026763 GN=EOO73_25305 PE=4 SV=1
MM1 pKa = 7.28TKK3 pKa = 10.22SISAIFLTCAVIATQGTLNAAVRR26 pKa = 11.84SDD28 pKa = 3.09IVALLEE34 pKa = 3.83VDD36 pKa = 3.8PAGSTLVVVDD46 pKa = 5.11GSAVNSTIKK55 pKa = 10.43AGKK58 pKa = 9.86VVLQANDD65 pKa = 4.14PNCDD69 pKa = 3.63PNVAPPCSYY78 pKa = 10.86VLKK81 pKa = 10.54KK82 pKa = 10.62LVIEE86 pKa = 4.26VTDD89 pKa = 4.25FSFKK93 pKa = 11.0DD94 pKa = 3.48EE95 pKa = 4.29NVTNGQFITTGPVNVVDD112 pKa = 4.34DD113 pKa = 4.33GGGIRR118 pKa = 11.84LPAGTPIGFAVTVGDD133 pKa = 4.53DD134 pKa = 3.22RR135 pKa = 11.84AGGIGSVAAPVLIQLDD151 pKa = 3.85VASQQMSLTGSFQGSLEE168 pKa = 4.13GFDD171 pKa = 3.25VAAAVAAQAQSPFVNVPPKK190 pKa = 10.41ISIEE194 pKa = 4.03KK195 pKa = 8.69TLQALEE201 pKa = 4.75CEE203 pKa = 5.06PIPLVASVTDD213 pKa = 3.76ATPAPNDD220 pKa = 3.99DD221 pKa = 3.54VFAPAWAIDD230 pKa = 3.61GVNQGFAPAGFASLAPGAHH249 pKa = 6.09FVQASVQDD257 pKa = 3.67FFGALAVDD265 pKa = 3.84SFVVNVSPDD274 pKa = 3.21EE275 pKa = 4.39APPVFTFVPSDD286 pKa = 3.39IKK288 pKa = 10.27TSSCGGLNIGVATAVDD304 pKa = 3.32NCGEE308 pKa = 4.2VEE310 pKa = 4.41VTSDD314 pKa = 3.81APAKK318 pKa = 9.6FAAGTTVVSWTARR331 pKa = 11.84DD332 pKa = 3.38ARR334 pKa = 11.84GNEE337 pKa = 3.88TVAQQVVTASLKK349 pKa = 10.83DD350 pKa = 3.72DD351 pKa = 4.6ASCCPVGSNVLVGSANNDD369 pKa = 3.32NLVGSSGPDD378 pKa = 3.26CILGLRR384 pKa = 11.84GQDD387 pKa = 3.42QIDD390 pKa = 3.76GGGGDD395 pKa = 4.46DD396 pKa = 4.71VISGGDD402 pKa = 3.26GDD404 pKa = 5.43DD405 pKa = 3.45ILRR408 pKa = 11.84AGAGNDD414 pKa = 4.0LVWAGPGQDD423 pKa = 3.58QLFGGIGDD431 pKa = 4.97DD432 pKa = 4.32LLSGNDD438 pKa = 3.6GDD440 pKa = 5.0DD441 pKa = 3.6LLRR444 pKa = 11.84GEE446 pKa = 5.14GGLDD450 pKa = 3.3TLVGGQGQDD459 pKa = 2.63ILYY462 pKa = 10.75GGDD465 pKa = 4.05DD466 pKa = 3.69NDD468 pKa = 3.97ILTGEE473 pKa = 4.71AGDD476 pKa = 3.95DD477 pKa = 3.62QLFGEE482 pKa = 5.07AGDD485 pKa = 4.49DD486 pKa = 4.0VLDD489 pKa = 5.07GGDD492 pKa = 4.31NNDD495 pKa = 3.32KK496 pKa = 10.01CTGGSGYY503 pKa = 9.05DD504 pKa = 3.38TMLSCTALDD513 pKa = 3.61TLEE516 pKa = 4.79NPGPPSFPGSPSYY529 pKa = 9.94EE530 pKa = 4.06VCEE533 pKa = 4.16CRR535 pKa = 11.84PNKK538 pKa = 10.31CADD541 pKa = 3.47CAAPVATCDD550 pKa = 4.04ASNGCAQIVQCVRR563 pKa = 11.84QAANCNLPHH572 pKa = 6.62EE573 pKa = 4.91CSALCEE579 pKa = 4.28SGRR582 pKa = 11.84TQSAIGAARR591 pKa = 11.84QLASCFGGCC600 pKa = 4.91

Molecular weight: 60.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q5XLE6|A0A4Q5XLE6_9DELT Uncharacterized protein OS=Myxococcales bacterium OX=2026763 GN=EOO73_00325 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84HH3 pKa = 5.35GGGWSSRR10 pKa = 11.84EE11 pKa = 3.65RR12 pKa = 11.84HH13 pKa = 5.61LSGHH17 pKa = 6.75AGRR20 pKa = 11.84GRR22 pKa = 11.84AGRR25 pKa = 11.84GRR27 pKa = 11.84AGRR30 pKa = 11.84GRR32 pKa = 11.84AGRR35 pKa = 11.84GRR37 pKa = 11.84AGRR40 pKa = 11.84GRR42 pKa = 11.84AGRR45 pKa = 11.84GRR47 pKa = 11.84AGRR50 pKa = 11.84GRR52 pKa = 11.84AGRR55 pKa = 11.84GRR57 pKa = 11.84AGRR60 pKa = 11.84GRR62 pKa = 11.84AGHH65 pKa = 6.92RR66 pKa = 11.84GRR68 pKa = 11.84GG69 pKa = 3.43

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7151

0

7151

2565783

28

4227

358.8

38.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.857 ± 0.042

1.189 ± 0.021

5.2 ± 0.023

6.267 ± 0.029

3.29 ± 0.017

8.878 ± 0.038

1.92 ± 0.014

3.231 ± 0.018

3.06 ± 0.025

10.81 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.662 ± 0.012

2.096 ± 0.018

5.922 ± 0.028

3.244 ± 0.015

7.637 ± 0.036

6.614 ± 0.026

5.066 ± 0.023

7.73 ± 0.023

1.234 ± 0.011

2.095 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski