Legionella drancourtii LLAP12
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3959 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9EKE5|G9EKE5_9GAMM Uncharacterized protein OS=Legionella drancourtii LLAP12 OX=658187 GN=LDG_5674 PE=4 SV=1
MM1 pKa = 7.79 IFDD4 pKa = 4.0 TGISGLQAASNNLDD18 pKa = 3.54 VIGNNIANSSTVGFKK33 pKa = 10.62 GSRR36 pKa = 11.84 ANFGDD41 pKa = 3.54 IYY43 pKa = 11.24 SFGGYY48 pKa = 9.26 GSSGTTPGGGVMLTSIQQSFASGTMTSTNNTLDD81 pKa = 3.75 LAISGNGFFILNNQGATSYY100 pKa = 11.16 SRR102 pKa = 11.84 AGQFTLNNDD111 pKa = 3.28 NYY113 pKa = 8.85 ITNSSGQFLTGYY125 pKa = 10.04 LADD128 pKa = 3.72 AAGNITGASGNIQVNMANLSPKK150 pKa = 9.51 ATTLVSTGLNLNSQSKK166 pKa = 9.89 PPAADD171 pKa = 2.98 WSGGAAPVTDD181 pKa = 4.44 TYY183 pKa = 11.95 NNTTSMTIYY192 pKa = 10.67 DD193 pKa = 4.08 SLGNSHH199 pKa = 6.36 VLSMYY204 pKa = 10.67 FIMADD209 pKa = 3.28 STATAGQPDD218 pKa = 3.81 AASPVGQTNQWYY230 pKa = 9.58 VAFQIDD236 pKa = 4.0 NQDD239 pKa = 3.07 VPSIVTPGNSSNLFAANFNSDD260 pKa = 3.25 GTFTSVQDD268 pKa = 3.75 TTGTAIPTNLIPLSYY283 pKa = 10.3 TLTNGANPLSLSIDD297 pKa = 3.64 LSSCTQFGSPFAVQSANASGYY318 pKa = 5.85 TTGSLAGLQVDD329 pKa = 3.83 AAGVISGSYY338 pKa = 11.07 SNGQTLAMGQIQLANFADD356 pKa = 4.56 LNGLQNIGNVCWTATATSGQALIGTGGTGSFGTLLSGRR394 pKa = 11.84 LEE396 pKa = 4.06 EE397 pKa = 4.6 SNVDD401 pKa = 3.15 ITSEE405 pKa = 3.99 LVDD408 pKa = 5.26 LIGAQRR414 pKa = 11.84 DD415 pKa = 3.97 FQANAQTIRR424 pKa = 11.84 AGDD427 pKa = 4.22 TITQTIINIRR437 pKa = 3.51
Molecular weight: 44.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.732
IPC_protein 3.757
Toseland 3.516
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.77
Rodwell 3.579
Grimsley 3.427
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.228
Thurlkill 3.592
EMBOSS 3.77
Sillero 3.884
Patrickios 0.439
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|G9EK50|G9EK50_9GAMM Histidine biosynthesis bifunctional protein HisIE OS=Legionella drancourtii LLAP12 OX=658187 GN=hisI PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.18 RR12 pKa = 11.84 KK13 pKa = 8.79 RR14 pKa = 11.84 DD15 pKa = 3.31 HH16 pKa = 6.72 GFRR19 pKa = 11.84 EE20 pKa = 4.28 RR21 pKa = 11.84 MATRR25 pKa = 11.84 AGRR28 pKa = 11.84 LVIKK32 pKa = 10.41 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.84 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 10.73
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.193
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.93
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.116
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3959
0
3959
1146871
37
4687
289.7
32.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.026 ± 0.042
1.183 ± 0.013
4.802 ± 0.029
5.916 ± 0.043
4.396 ± 0.028
5.853 ± 0.045
2.598 ± 0.019
7.303 ± 0.038
6.256 ± 0.046
10.969 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.439 ± 0.022
4.986 ± 0.031
4.028 ± 0.024
4.769 ± 0.039
4.196 ± 0.029
6.438 ± 0.03
5.393 ± 0.032
5.897 ± 0.036
1.12 ± 0.014
3.432 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here