Beauveria bassiana polymycovirus 1
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V1IEY9|A0A1V1IEY9_9VIRU RNA dependent RNA polymerase OS=Beauveria bassiana polymycovirus 1 OX=1740646 GN=RdRp PE=4 SV=1
MM1 pKa = 7.89 ADD3 pKa = 3.79 LTRR6 pKa = 11.84 LSAIAVDD13 pKa = 4.05 VGSPAPAVAAVLDD26 pKa = 4.18 LFGVSDD32 pKa = 3.81 PTGQCLSSAQVMSVVEE48 pKa = 4.73 WIAGFPLQSAPGLMPAYY65 pKa = 9.55 VFEE68 pKa = 4.37 YY69 pKa = 10.21 VKK71 pKa = 10.92 SKK73 pKa = 10.86 AGSLVASAGVCDD85 pKa = 4.07 DD86 pKa = 2.96 VAYY89 pKa = 10.26 FLRR92 pKa = 11.84 HH93 pKa = 5.74 FEE95 pKa = 4.87 APDD98 pKa = 3.1 EE99 pKa = 4.9 HH100 pKa = 6.07 YY101 pKa = 9.28 TKK103 pKa = 10.22 SGRR106 pKa = 11.84 SARR109 pKa = 11.84 VAQVALNKK117 pKa = 9.41 PLKK120 pKa = 10.61 GDD122 pKa = 3.81 GAHH125 pKa = 6.94 LGAVEE130 pKa = 4.76 LALLDD135 pKa = 3.56 AVTRR139 pKa = 11.84 VGYY142 pKa = 9.93 VGPAGALASVARR154 pKa = 11.84 LRR156 pKa = 11.84 TMEE159 pKa = 4.11 VLRR162 pKa = 11.84 HH163 pKa = 4.85 YY164 pKa = 10.95 AVDD167 pKa = 3.42 VRR169 pKa = 11.84 QRR171 pKa = 11.84 ACAQYY176 pKa = 10.67 RR177 pKa = 11.84 VTCQVCLPGRR187 pKa = 11.84 DD188 pKa = 3.82 SYY190 pKa = 11.84 PLITPWCVSRR200 pKa = 11.84 QSATICAGVILASVGGEE217 pKa = 3.64 AATKK221 pKa = 9.61 AVQRR225 pKa = 11.84 RR226 pKa = 11.84 AVTVASHH233 pKa = 7.06 AARR236 pKa = 11.84 IGLDD240 pKa = 3.1 AVRR243 pKa = 11.84 AIVAPACRR251 pKa = 11.84 LVAEE255 pKa = 4.44 YY256 pKa = 11.03 SFDD259 pKa = 3.68 PVRR262 pKa = 11.84 LVLTDD267 pKa = 3.5 RR268 pKa = 11.84 YY269 pKa = 10.28 GRR271 pKa = 11.84 VRR273 pKa = 11.84 EE274 pKa = 4.38 RR275 pKa = 11.84 ITSRR279 pKa = 11.84 SPAATVAFAYY289 pKa = 7.65 LTSRR293 pKa = 11.84 GSADD297 pKa = 3.79 PLLAMASEE305 pKa = 4.56 RR306 pKa = 11.84 VEE308 pKa = 4.08 RR309 pKa = 11.84 MGPVEE314 pKa = 5.63 LLPQCVQDD322 pKa = 3.3 YY323 pKa = 9.09 WNQSEE328 pKa = 5.53 DD329 pKa = 3.49 IVGMLSLFCRR339 pKa = 11.84 ALHH342 pKa = 5.17 LTRR345 pKa = 11.84 VEE347 pKa = 4.01 RR348 pKa = 11.84 EE349 pKa = 3.68 EE350 pKa = 4.05 EE351 pKa = 3.85 EE352 pKa = 4.31 RR353 pKa = 11.84 GRR355 pKa = 11.84 DD356 pKa = 3.77 VNKK359 pKa = 10.41 GPCLGYY365 pKa = 10.38 LAVGRR370 pKa = 11.84 KK371 pKa = 9.24 KK372 pKa = 10.21 RR373 pKa = 11.84 DD374 pKa = 3.22 RR375 pKa = 11.84 NTASCINRR383 pKa = 11.84 LCEE386 pKa = 3.97 LMCEE390 pKa = 4.22 VRR392 pKa = 11.84 ALGQSPEE399 pKa = 4.03 DD400 pKa = 3.35 LLFVVEE406 pKa = 4.62 WGGQIDD412 pKa = 4.22 SAAVPAFCAMARR424 pKa = 11.84 VDD426 pKa = 3.63 CAFDD430 pKa = 3.44 VGASGVDD437 pKa = 2.97 IGGVADD443 pKa = 4.12 PGKK446 pKa = 10.3 SLEE449 pKa = 4.19 VHH451 pKa = 6.87 HH452 pKa = 5.79 YY453 pKa = 8.72 TAVLTHH459 pKa = 6.27 RR460 pKa = 11.84 VGRR463 pKa = 11.84 TLSVCCEE470 pKa = 3.58 VPYY473 pKa = 11.13 AKK475 pKa = 10.46 DD476 pKa = 2.84 SGVNDD481 pKa = 5.17 RR482 pKa = 11.84 IRR484 pKa = 11.84 TVVDD488 pKa = 3.44 AVGEE492 pKa = 4.32 GVKK495 pKa = 10.8 GFVYY499 pKa = 10.66 VSGGIPAVASMSAEE513 pKa = 3.66 QSVNDD518 pKa = 3.82 AHH520 pKa = 7.93 SDD522 pKa = 2.94 IYY524 pKa = 11.23 AALVGDD530 pKa = 4.83 APLKK534 pKa = 10.79 CILFSTDD541 pKa = 4.56 LIIPPACPHH550 pKa = 6.78 CEE552 pKa = 4.06 TVDD555 pKa = 3.81 LDD557 pKa = 3.61 HH558 pKa = 7.02 FLASRR563 pKa = 11.84 GVLSEE568 pKa = 4.14 DD569 pKa = 3.48 CRR571 pKa = 11.84 EE572 pKa = 4.02 CTVFGRR578 pKa = 11.84 ALRR581 pKa = 11.84 LMSEE585 pKa = 4.45 LMSTPGVRR593 pKa = 11.84 LVKK596 pKa = 9.68 TRR598 pKa = 11.84 SAFAHH603 pKa = 6.02 NSQFDD608 pKa = 3.97 LEE610 pKa = 4.43 YY611 pKa = 11.14 SSLLRR616 pKa = 11.84 NTLTDD621 pKa = 2.84 SSSAVDD627 pKa = 3.36 SAAFSTGARR636 pKa = 11.84 NRR638 pKa = 11.84 TWGGGMPVGSDD649 pKa = 3.62 DD650 pKa = 4.97 LVAAQAIGGTVDD662 pKa = 3.16 GEE664 pKa = 4.48 FYY666 pKa = 11.24 DD667 pKa = 3.74 RR668 pKa = 11.84 MVGIRR673 pKa = 11.84 RR674 pKa = 11.84 NVYY677 pKa = 10.19 ALLAGGAQPRR687 pKa = 11.84 PALADD692 pKa = 3.38 DD693 pKa = 3.96 TFSDD697 pKa = 4.09 VVRR700 pKa = 11.84 SVGAA704 pKa = 3.55
Molecular weight: 74.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.68
IPC2_protein 5.741
IPC_protein 5.779
Toseland 5.995
ProMoST 6.122
Dawson 6.033
Bjellqvist 6.059
Wikipedia 6.033
Rodwell 6.02
Grimsley 6.071
Solomon 6.033
Lehninger 6.033
Nozaki 6.3
DTASelect 6.478
Thurlkill 6.478
EMBOSS 6.44
Sillero 6.402
Patrickios 3.948
IPC_peptide 6.046
IPC2_peptide 6.402
IPC2.peptide.svr19 6.334
Protein with the highest isoelectric point:
>tr|A0A1V1IEY9|A0A1V1IEY9_9VIRU RNA dependent RNA polymerase OS=Beauveria bassiana polymycovirus 1 OX=1740646 GN=RdRp PE=4 SV=1
MM1 pKa = 7.24 SVSTVTSEE9 pKa = 3.78 IAHH12 pKa = 5.31 VQVACRR18 pKa = 11.84 PVSEE22 pKa = 4.65 MAPHH26 pKa = 6.9 LQRR29 pKa = 11.84 ASQDD33 pKa = 3.57 TVQGALKK40 pKa = 9.67 WVADD44 pKa = 3.88 KK45 pKa = 11.14 YY46 pKa = 10.93 SRR48 pKa = 11.84 GGVRR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 NLTVHH59 pKa = 6.54 LSGVRR64 pKa = 11.84 GRR66 pKa = 11.84 ADD68 pKa = 3.45 DD69 pKa = 3.78 VVLIPSVIEE78 pKa = 4.08 ANSVLPEE85 pKa = 4.33 APVHH89 pKa = 5.77 RR90 pKa = 11.84 APYY93 pKa = 10.07 VDD95 pKa = 3.88 IGGDD99 pKa = 3.65 SADD102 pKa = 3.39 SRR104 pKa = 11.84 YY105 pKa = 10.6 EE106 pKa = 3.9 GLKK109 pKa = 11.06 GLDD112 pKa = 2.93 KK113 pKa = 11.06 VQNKK117 pKa = 7.83 GAKK120 pKa = 9.65 GGAEE124 pKa = 4.24 FEE126 pKa = 4.54 RR127 pKa = 11.84 KK128 pKa = 8.72 SSRR131 pKa = 11.84 MEE133 pKa = 3.4 GAIRR137 pKa = 11.84 KK138 pKa = 9.45 RR139 pKa = 11.84 GMLRR143 pKa = 11.84 NPSFQVCRR151 pKa = 11.84 YY152 pKa = 7.8 QLPFSYY158 pKa = 9.95 GYY160 pKa = 9.63 KK161 pKa = 9.77 ARR163 pKa = 11.84 RR164 pKa = 11.84 VTTEE168 pKa = 3.32 RR169 pKa = 11.84 HH170 pKa = 5.28 LLGPTLDD177 pKa = 3.65 ALHH180 pKa = 6.45 RR181 pKa = 11.84 AKK183 pKa = 10.43 RR184 pKa = 11.84 RR185 pKa = 11.84 PGYY188 pKa = 8.54 PQVVKK193 pKa = 10.91 DD194 pKa = 4.21 SLDD197 pKa = 3.55 EE198 pKa = 4.31 AVDD201 pKa = 3.71 LGDD204 pKa = 4.04 YY205 pKa = 7.41 TTHH208 pKa = 7.86 DD209 pKa = 4.61 PDD211 pKa = 3.88 TLHH214 pKa = 7.07 KK215 pKa = 10.59 RR216 pKa = 11.84 FLEE219 pKa = 4.4 YY220 pKa = 9.88 VHH222 pKa = 6.52 EE223 pKa = 4.29 RR224 pKa = 11.84 VEE226 pKa = 4.57 GVSADD231 pKa = 3.46 TKK233 pKa = 10.7 TDD235 pKa = 3.57 FLVAVQVMWRR245 pKa = 11.84 VWSDD249 pKa = 2.85 AGVAAEE255 pKa = 4.81 ARR257 pKa = 11.84 PFSDD261 pKa = 3.57 ATPEE265 pKa = 4.1 GLDD268 pKa = 4.15 AMFTTGNAGEE278 pKa = 4.07 YY279 pKa = 9.0 RR280 pKa = 11.84 KK281 pKa = 10.3 HH282 pKa = 5.89 GVEE285 pKa = 4.0 SRR287 pKa = 11.84 RR288 pKa = 11.84 DD289 pKa = 3.48 PRR291 pKa = 11.84 ALDD294 pKa = 3.42 MLSKK298 pKa = 10.13 HH299 pKa = 5.07 VQGFTVAGRR308 pKa = 11.84 NMPTGMSPPAFVDD321 pKa = 3.4 RR322 pKa = 11.84 LDD324 pKa = 3.9 HH325 pKa = 5.66 VTTSFGKK332 pKa = 10.4 RR333 pKa = 11.84 EE334 pKa = 4.0 PKK336 pKa = 9.37 KK337 pKa = 10.55 AKK339 pKa = 9.36 IVNGQRR345 pKa = 11.84 VAPVPRR351 pKa = 11.84 FIFNPSPCSYY361 pKa = 11.68 AMGAFLHH368 pKa = 6.68 SDD370 pKa = 3.1 ISHH373 pKa = 7.27 ALQDD377 pKa = 3.95 RR378 pKa = 11.84 DD379 pKa = 3.88 PLHH382 pKa = 6.87 GPGFGPGRR390 pKa = 11.84 GRR392 pKa = 11.84 AVKK395 pKa = 8.62 FTRR398 pKa = 11.84 IVEE401 pKa = 4.19 AACPDD406 pKa = 3.56 GSRR409 pKa = 11.84 LRR411 pKa = 11.84 GGCKK415 pKa = 9.86 AVMSDD420 pKa = 3.81 IEE422 pKa = 4.16 KK423 pKa = 9.89 WDD425 pKa = 3.74 ANMSEE430 pKa = 4.26 FLIGTSFDD438 pKa = 3.46 ALEE441 pKa = 4.33 AFVDD445 pKa = 3.94 KK446 pKa = 11.26 SKK448 pKa = 10.79 LAPLDD453 pKa = 3.47 RR454 pKa = 11.84 ASRR457 pKa = 11.84 EE458 pKa = 3.73 ASCRR462 pKa = 11.84 YY463 pKa = 7.43 MRR465 pKa = 11.84 RR466 pKa = 11.84 TLMEE470 pKa = 4.42 KK471 pKa = 10.29 LVEE474 pKa = 4.41 HH475 pKa = 6.74 PSGYY479 pKa = 10.2 LVHH482 pKa = 7.29 LYY484 pKa = 10.7 GCMPSGSFYY493 pKa = 10.81 TSLLNTVANTLLALALLARR512 pKa = 11.84 RR513 pKa = 11.84 MRR515 pKa = 11.84 LAEE518 pKa = 4.07 KK519 pKa = 9.96 PIDD522 pKa = 3.45 IAAMARR528 pKa = 11.84 SADD531 pKa = 3.75 GALLSYY537 pKa = 11.04 GDD539 pKa = 3.8 NQLIITSLFEE549 pKa = 3.97 AHH551 pKa = 6.01 GVEE554 pKa = 5.07 YY555 pKa = 11.16 DD556 pKa = 3.56 MDD558 pKa = 3.85 DD559 pKa = 3.48 HH560 pKa = 8.55 AEE562 pKa = 4.07 LLSEE566 pKa = 4.16 VGMRR570 pKa = 11.84 LKK572 pKa = 10.52 KK573 pKa = 10.73 DD574 pKa = 3.23 EE575 pKa = 4.62 TEE577 pKa = 3.95 VSDD580 pKa = 3.56 RR581 pKa = 11.84 LDD583 pKa = 3.92 RR584 pKa = 11.84 IRR586 pKa = 11.84 FCSRR590 pKa = 11.84 AVVRR594 pKa = 11.84 TPAGMAITRR603 pKa = 11.84 PHH605 pKa = 6.62 GDD607 pKa = 3.07 VVAKK611 pKa = 10.17 LVARR615 pKa = 11.84 PTEE618 pKa = 3.76 NSVDD622 pKa = 3.44 DD623 pKa = 4.17 KK624 pKa = 11.6 LYY626 pKa = 11.07 VRR628 pKa = 11.84 ALMADD633 pKa = 3.45 YY634 pKa = 10.18 MGVDD638 pKa = 4.52 PIVHH642 pKa = 7.12 RR643 pKa = 11.84 ILADD647 pKa = 3.08 VDD649 pKa = 3.15 ATIRR653 pKa = 11.84 VTIDD657 pKa = 2.81 AVRR660 pKa = 11.84 ASEE663 pKa = 4.04 RR664 pKa = 11.84 HH665 pKa = 5.91 RR666 pKa = 11.84 DD667 pKa = 3.77 TLRR670 pKa = 11.84 SAASQVFGRR679 pKa = 11.84 KK680 pKa = 9.5 DD681 pKa = 3.31 DD682 pKa = 4.18 DD683 pKa = 5.39 AIAATAQLMAKK694 pKa = 9.97 AVIDD698 pKa = 3.64 RR699 pKa = 11.84 RR700 pKa = 11.84 VLLEE704 pKa = 3.68 LTQPRR709 pKa = 11.84 SVNDD713 pKa = 3.06 VDD715 pKa = 4.98 SVLEE719 pKa = 3.99 RR720 pKa = 11.84 TGKK723 pKa = 7.83 VTGVRR728 pKa = 11.84 FRR730 pKa = 11.84 QRR732 pKa = 11.84 FAASVGIQTDD742 pKa = 3.94 VPSLVGPAAWLASQDD757 pKa = 3.41 EE758 pKa = 4.33 QQYY761 pKa = 11.14 LDD763 pKa = 3.59 YY764 pKa = 10.88 LVRR767 pKa = 11.84 TDD769 pKa = 3.46 QVGVMHH775 pKa = 7.4
Molecular weight: 85.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.04
IPC2_protein 8.141
IPC_protein 8.039
Toseland 8.302
ProMoST 8.565
Dawson 8.858
Bjellqvist 8.931
Wikipedia 8.931
Rodwell 8.931
Grimsley 8.463
Solomon 8.946
Lehninger 8.931
Nozaki 9.048
DTASelect 8.77
Thurlkill 8.843
EMBOSS 9.019
Sillero 9.077
Patrickios 4.8
IPC_peptide 8.946
IPC2_peptide 7.732
IPC2.peptide.svr19 7.719
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2395
306
775
598.8
64.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.608 ± 1.086
1.879 ± 0.549
7.516 ± 0.407
4.635 ± 0.05
3.215 ± 0.432
8.058 ± 0.505
2.255 ± 0.345
3.006 ± 0.104
3.048 ± 0.562
8.225 ± 0.39
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.296 ± 0.3
1.461 ± 0.168
5.136 ± 0.335
2.756 ± 0.164
8.894 ± 0.354
7.432 ± 0.237
4.676 ± 0.19
10.647 ± 0.857
0.752 ± 0.052
2.505 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here