Beihai tombus-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFW4|A0A1L3KFW4_9VIRU Capsid protein OS=Beihai tombus-like virus 3 OX=1922724 PE=3 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84RR3 pKa = 11.84KK4 pKa = 8.07TNNARR9 pKa = 11.84RR10 pKa = 11.84VSAPIANGLRR20 pKa = 11.84TSGGRR25 pKa = 11.84PQMTPAGAGSTRR37 pKa = 11.84VRR39 pKa = 11.84HH40 pKa = 6.01RR41 pKa = 11.84EE42 pKa = 3.48FVQNITSDD50 pKa = 3.4HH51 pKa = 5.87TFGSEE56 pKa = 4.42LLALGINVGDD66 pKa = 3.84TKK68 pKa = 10.68TFPWLSKK75 pKa = 9.83IANGYY80 pKa = 8.17EE81 pKa = 4.3RR82 pKa = 11.84YY83 pKa = 9.06CVHH86 pKa = 6.91SMTISYY92 pKa = 10.38EE93 pKa = 4.08PFVSTTEE100 pKa = 3.74TGAILMLADD109 pKa = 4.65YY110 pKa = 10.28DD111 pKa = 4.49PNDD114 pKa = 4.32PPPTSKK120 pKa = 11.34SNMLNSMGASRR131 pKa = 11.84SAVWMNTTMPLDD143 pKa = 4.0RR144 pKa = 11.84KK145 pKa = 9.58EE146 pKa = 4.55LSYY149 pKa = 11.46DD150 pKa = 2.96DD151 pKa = 4.48HH152 pKa = 8.62LFVRR156 pKa = 11.84HH157 pKa = 4.97QTRR160 pKa = 11.84EE161 pKa = 4.01TLTEE165 pKa = 3.77NLKK168 pKa = 10.89LYY170 pKa = 10.68DD171 pKa = 3.56VGTVFVGVTDD181 pKa = 3.97NEE183 pKa = 4.46PVSGTKK189 pKa = 9.84QYY191 pKa = 11.11GEE193 pKa = 3.71VWITYY198 pKa = 9.92DD199 pKa = 3.29VTLMVPAFHH208 pKa = 6.79TSNPNSAEE216 pKa = 4.09TKK218 pKa = 9.92VNGAIAPDD226 pKa = 3.95FLGGIQSSNRR236 pKa = 11.84AAMKK240 pKa = 10.54DD241 pKa = 3.33GSAVNFSTAHH251 pKa = 6.05NGDD254 pKa = 3.77DD255 pKa = 3.39QVAITFHH262 pKa = 6.55EE263 pKa = 4.85PFTGLLYY270 pKa = 10.06MEE272 pKa = 4.29QSGYY276 pKa = 11.54ALDD279 pKa = 4.46DD280 pKa = 3.67TTHH283 pKa = 5.6VQLEE287 pKa = 4.68PEE289 pKa = 4.19TSPTDD294 pKa = 3.21GWISKK299 pKa = 9.15LAKK302 pKa = 10.32LGGITADD309 pKa = 3.75YY310 pKa = 10.08FFQEE314 pKa = 4.38NTWKK318 pKa = 10.93YY319 pKa = 10.34LMEE322 pKa = 4.28VVADD326 pKa = 3.93AGEE329 pKa = 4.54SIVFSALGVGAADD342 pKa = 3.03IATWIGEE349 pKa = 4.54VAWLLAPYY357 pKa = 10.77AEE359 pKa = 4.26TLMFAPLLVLNKK371 pKa = 10.14ASAIDD376 pKa = 3.48VRR378 pKa = 11.84ARR380 pKa = 11.84LQARR384 pKa = 11.84SLNGILPANEE394 pKa = 3.77VLPRR398 pKa = 11.84LDD400 pKa = 3.04WVLQQSNQDD409 pKa = 3.48PEE411 pKa = 4.18EE412 pKa = 4.0

Molecular weight: 45.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFW4|A0A1L3KFW4_9VIRU Capsid protein OS=Beihai tombus-like virus 3 OX=1922724 PE=3 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84ASLFPKK8 pKa = 9.0RR9 pKa = 11.84TTGEE13 pKa = 4.02VVGLVDD19 pKa = 4.92GPVPTPQEE27 pKa = 3.89VRR29 pKa = 11.84GGPFSWDD36 pKa = 3.62KK37 pKa = 11.48AWLRR41 pKa = 11.84CMNTLKK47 pKa = 10.78QNLQSEE53 pKa = 4.65IVGCVNHH60 pKa = 5.96NHH62 pKa = 5.67VDD64 pKa = 3.7PRR66 pKa = 11.84PEE68 pKa = 3.34WEE70 pKa = 3.87IAVTEE75 pKa = 4.39GLASTGNVIINNTIRR90 pKa = 11.84STGFNVSVTAGGAVGTVGVICGMGVVVSYY119 pKa = 11.1APLVAGVAATWAVSALRR136 pKa = 11.84RR137 pKa = 11.84EE138 pKa = 4.04RR139 pKa = 11.84SHH141 pKa = 7.46AVDD144 pKa = 3.28VTLSVLNDD152 pKa = 3.52DD153 pKa = 5.11VRR155 pKa = 11.84IGDD158 pKa = 4.35LKK160 pKa = 10.9AAGGKK165 pKa = 9.43EE166 pKa = 3.93EE167 pKa = 5.77AGGEE171 pKa = 3.92DD172 pKa = 3.44QAAVDD177 pKa = 4.56SEE179 pKa = 4.79DD180 pKa = 3.7NPAEE184 pKa = 4.11STRR187 pKa = 11.84EE188 pKa = 3.59RR189 pKa = 11.84RR190 pKa = 11.84EE191 pKa = 3.58FEE193 pKa = 3.76VRR195 pKa = 11.84RR196 pKa = 11.84RR197 pKa = 11.84KK198 pKa = 9.86PFFGPQPRR206 pKa = 11.84NHH208 pKa = 5.57GRR210 pKa = 11.84IPVMAGEE217 pKa = 4.24VANLLKK223 pKa = 10.53LRR225 pKa = 11.84HH226 pKa = 6.07IGLRR230 pKa = 11.84DD231 pKa = 3.39TPEE234 pKa = 3.39NRR236 pKa = 11.84FLIRR240 pKa = 11.84ADD242 pKa = 3.04AGKK245 pKa = 10.07RR246 pKa = 11.84AEE248 pKa = 3.84ALRR251 pKa = 11.84RR252 pKa = 11.84EE253 pKa = 4.84GEE255 pKa = 4.31RR256 pKa = 11.84PWNTVRR262 pKa = 11.84NAEE265 pKa = 4.19LLSIAMHH272 pKa = 6.45ASEE275 pKa = 5.21MYY277 pKa = 9.72WILSRR282 pKa = 11.84DD283 pKa = 3.36EE284 pKa = 4.51EE285 pKa = 4.91YY286 pKa = 11.18VGEE289 pKa = 4.52LYY291 pKa = 10.68SEE293 pKa = 4.22SLLRR297 pKa = 11.84GLRR300 pKa = 11.84RR301 pKa = 11.84RR302 pKa = 11.84RR303 pKa = 11.84NRR305 pKa = 11.84WVSSSVSSKK314 pKa = 11.17

Molecular weight: 34.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1270

314

544

423.3

47.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.165 ± 1.394

1.339 ± 0.587

5.827 ± 0.659

6.929 ± 0.612

3.858 ± 0.687

7.953 ± 0.458

2.047 ± 0.062

4.646 ± 0.479

4.803 ± 1.288

8.189 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.992 ± 0.395

4.409 ± 0.702

5.039 ± 0.131

2.598 ± 0.362

7.48 ± 1.39

7.008 ± 0.126

5.197 ± 1.568

7.323 ± 1.193

1.732 ± 0.252

3.465 ± 0.928
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski