Desertihabitans brevis
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3682 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A367YYA9|A0A367YYA9_9ACTN DNA-(apurinic or apyrimidinic site) lyase OS=Desertihabitans brevis OX=2268447 GN=DT076_08310 PE=3 SV=1
MM1 pKa = 7.76 ALTACGTRR9 pKa = 11.84 AEE11 pKa = 4.36 EE12 pKa = 4.63 GGGGAEE18 pKa = 4.39 GGAGGGGGEE27 pKa = 4.57 TKK29 pKa = 9.84 TAVIGVVAPLSGDD42 pKa = 3.91 LAPLGLGIQHH52 pKa = 5.84 SVEE55 pKa = 4.26 LAVQQANEE63 pKa = 4.15 SGAIPGWTLEE73 pKa = 4.09 VQPEE77 pKa = 4.04 DD78 pKa = 4.06 DD79 pKa = 3.7 QATPDD84 pKa = 3.46 VGRR87 pKa = 11.84 NAATSLSSDD96 pKa = 3.3 ADD98 pKa = 3.72 VVAMVGPLNSSVGQAVQPVFDD119 pKa = 4.21 AAGILQVSPANTNPSLTRR137 pKa = 11.84 GANFDD142 pKa = 3.78 TAPEE146 pKa = 4.14 RR147 pKa = 11.84 TYY149 pKa = 9.06 PTYY152 pKa = 10.77 FRR154 pKa = 11.84 TCTTDD159 pKa = 3.68 SIQGPFAARR168 pKa = 11.84 YY169 pKa = 8.76 LYY171 pKa = 7.87 EE172 pKa = 3.93 TAGITRR178 pKa = 11.84 IATVHH183 pKa = 6.48 DD184 pKa = 4.01 KK185 pKa = 10.79 KK186 pKa = 11.08 AYY188 pKa = 9.12 GQGLVEE194 pKa = 4.32 AFSAEE199 pKa = 3.98 FEE201 pKa = 4.33 EE202 pKa = 5.12 LGGEE206 pKa = 3.96 IVAAEE211 pKa = 4.44 TINPEE216 pKa = 4.18 DD217 pKa = 4.4 DD218 pKa = 3.56 NFSAVITSVGSGDD231 pKa = 4.16 PEE233 pKa = 3.64 ALYY236 pKa = 11.11 YY237 pKa = 10.59 GGEE240 pKa = 4.23 YY241 pKa = 9.09 PQSGPLSQQMKK252 pKa = 10.23 AAGLDD257 pKa = 3.38 IPLMGGDD264 pKa = 3.66 GMYY267 pKa = 10.35 SPEE270 pKa = 4.3 YY271 pKa = 10.16 IEE273 pKa = 5.23 LAGEE277 pKa = 3.98 QSEE280 pKa = 4.86 GDD282 pKa = 3.48 LATSVGAPVEE292 pKa = 4.39 DD293 pKa = 4.9 LPSAQQFVTDD303 pKa = 3.71 YY304 pKa = 9.88 EE305 pKa = 4.35 AAGFPDD311 pKa = 4.53 PYY313 pKa = 9.75 EE314 pKa = 4.59 AYY316 pKa = 10.19 GAYY319 pKa = 10.2 SYY321 pKa = 11.33 DD322 pKa = 3.3 AAQAVIEE329 pKa = 4.07 ALKK332 pKa = 10.89 VSLPEE337 pKa = 4.8 AEE339 pKa = 4.22 TAEE342 pKa = 4.52 DD343 pKa = 3.39 ARR345 pKa = 11.84 EE346 pKa = 4.12 ATVEE350 pKa = 3.87 AMASVSFEE358 pKa = 3.98 GATGPVAFDD367 pKa = 3.34 EE368 pKa = 5.33 FGDD371 pKa = 3.55 NTTRR375 pKa = 11.84 ILTAYY380 pKa = 9.08 KK381 pKa = 10.37 VEE383 pKa = 4.25 GGAWVADD390 pKa = 3.57 QTDD393 pKa = 3.69 EE394 pKa = 5.47 FEE396 pKa = 4.58 DD397 pKa = 3.64
Molecular weight: 40.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.732
IPC_protein 3.719
Toseland 3.528
ProMoST 3.834
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.579
Rodwell 3.554
Grimsley 3.439
Solomon 3.681
Lehninger 3.643
Nozaki 3.795
DTASelect 3.973
Thurlkill 3.554
EMBOSS 3.592
Sillero 3.834
Patrickios 0.947
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|A0A367YR83|A0A367YR83_9ACTN ABC transporter permease OS=Desertihabitans brevis OX=2268447 GN=DT076_16485 PE=3 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.31 VRR4 pKa = 11.84 NSLRR8 pKa = 11.84 ALKK11 pKa = 9.65 KK12 pKa = 10.17 IPGAQIVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GRR24 pKa = 11.84 TFVINKK30 pKa = 8.79 RR31 pKa = 11.84 NPRR34 pKa = 11.84 FKK36 pKa = 10.78 ARR38 pKa = 11.84 QGG40 pKa = 3.28
Molecular weight: 4.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.155
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.618
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.34
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3682
0
3682
1212445
31
2815
329.3
35.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.039 ± 0.05
0.711 ± 0.011
6.201 ± 0.037
5.978 ± 0.036
2.642 ± 0.026
9.343 ± 0.038
2.158 ± 0.022
2.773 ± 0.033
1.294 ± 0.021
10.737 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.64 ± 0.017
1.545 ± 0.023
6.164 ± 0.037
3.024 ± 0.022
8.277 ± 0.055
5.092 ± 0.03
6.188 ± 0.036
9.712 ± 0.041
1.642 ± 0.017
1.84 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here