Streptococcus satellite phage Javan615
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZT48|A0A4D5ZT48_9VIRU PriCT_1 domain-containing protein OS=Streptococcus satellite phage Javan615 OX=2558785 GN=JavanS615_0007 PE=4 SV=1
MM1 pKa = 7.37 TEE3 pKa = 4.19 KK4 pKa = 10.67 FNLPAEE10 pKa = 4.3 RR11 pKa = 11.84 AKK13 pKa = 11.12 SFGLEE18 pKa = 3.88 LEE20 pKa = 4.19 EE21 pKa = 5.35 AYY23 pKa = 9.57 NTMVVFSLEE32 pKa = 4.16 NKK34 pKa = 9.48 FDD36 pKa = 4.17 CYY38 pKa = 10.58 PPQDD42 pKa = 3.48 RR43 pKa = 11.84 KK44 pKa = 10.8 KK45 pKa = 10.55 LEE47 pKa = 4.16 SVLEE51 pKa = 4.03 FLMDD55 pKa = 3.71 TTDD58 pKa = 2.47 MWMNGQIMVSSQEE71 pKa = 3.73 RR72 pKa = 11.84 GVNEE76 pKa = 4.04 KK77 pKa = 10.52 KK78 pKa = 10.75
Molecular weight: 9.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.884
IPC2_protein 4.889
IPC_protein 4.685
Toseland 4.546
ProMoST 4.762
Dawson 4.609
Bjellqvist 4.762
Wikipedia 4.444
Rodwell 4.52
Grimsley 4.457
Solomon 4.596
Lehninger 4.558
Nozaki 4.724
DTASelect 4.8
Thurlkill 4.533
EMBOSS 4.469
Sillero 4.787
Patrickios 4.253
IPC_peptide 4.609
IPC2_peptide 4.787
IPC2.peptide.svr19 4.752
Protein with the highest isoelectric point:
>tr|A0A4D5ZSK7|A0A4D5ZSK7_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan615 OX=2558785 GN=JavanS615_0002 PE=4 SV=1
MM1 pKa = 7.78 KK2 pKa = 10.26 INEE5 pKa = 4.22 IKK7 pKa = 10.67 KK8 pKa = 9.76 KK9 pKa = 10.54 DD10 pKa = 3.3 GSTVYY15 pKa = 10.36 RR16 pKa = 11.84 ANIYY20 pKa = 10.62 LGVDD24 pKa = 3.95 VITGKK29 pKa = 10.74 KK30 pKa = 7.55 VTTKK34 pKa = 9.32 VTARR38 pKa = 11.84 TKK40 pKa = 10.91 KK41 pKa = 9.48 EE42 pKa = 4.24 LKK44 pKa = 9.67 TKK46 pKa = 10.1 AQQAQFDD53 pKa = 4.54 FKK55 pKa = 11.59 ANGSTRR61 pKa = 11.84 YY62 pKa = 9.72 KK63 pKa = 9.99 EE64 pKa = 4.21 VAIEE68 pKa = 4.25 TYY70 pKa = 10.63 EE71 pKa = 3.95 EE72 pKa = 4.45 LAISWWDD79 pKa = 3.38 SYY81 pKa = 11.84 KK82 pKa = 10.19 NTVKK86 pKa = 10.93 ANTRR90 pKa = 11.84 NTQKK94 pKa = 11.26 GLLNNHH100 pKa = 5.72 VLPLFGEE107 pKa = 4.67 FKK109 pKa = 10.56 LDD111 pKa = 3.59 KK112 pKa = 10.06 LTTPLIQSTMNKK124 pKa = 9.37 LANSANTGEE133 pKa = 4.27 AGAYY137 pKa = 8.81 LHH139 pKa = 6.29 YY140 pKa = 11.12 SKK142 pKa = 10.41 IHH144 pKa = 5.29 TLNKK148 pKa = 10.12 RR149 pKa = 11.84 ILQYY153 pKa = 11.17 GVILQVIPTNPANNVVLPRR172 pKa = 11.84 NTQKK176 pKa = 11.1 DD177 pKa = 3.22 KK178 pKa = 10.56 KK179 pKa = 10.89 AKK181 pKa = 9.81 VKK183 pKa = 10.6 HH184 pKa = 5.76 FNNDD188 pKa = 2.8 EE189 pKa = 4.08 LKK191 pKa = 10.82 QFLTYY196 pKa = 10.53 LDD198 pKa = 4.07 SLDD201 pKa = 3.34 NSKK204 pKa = 11.04 YY205 pKa = 10.38 KK206 pKa = 10.64 NYY208 pKa = 10.65 YY209 pKa = 10.47 DD210 pKa = 3.03 ITLYY214 pKa = 10.98 KK215 pKa = 10.3 FLLATGCRR223 pKa = 11.84 VNEE226 pKa = 3.97 ALALSWSDD234 pKa = 2.86 IDD236 pKa = 4.49 LDD238 pKa = 3.79 NSVVHH243 pKa = 5.55 ITKK246 pKa = 9.12 TLNCEE251 pKa = 4.28 LEE253 pKa = 4.31 LNSPKK258 pKa = 10.53 SKK260 pKa = 10.28 SGYY263 pKa = 9.62 RR264 pKa = 11.84 DD265 pKa = 2.83 IDD267 pKa = 3.15 IDD269 pKa = 3.87 KK270 pKa = 9.04 QTVTMMKK277 pKa = 9.91 RR278 pKa = 11.84 YY279 pKa = 7.4 QHH281 pKa = 6.32 KK282 pKa = 8.56 QTQEE286 pKa = 3.26 SWKK289 pKa = 10.31 LGRR292 pKa = 11.84 TEE294 pKa = 3.98 TVVFSDD300 pKa = 5.91 FINAYY305 pKa = 9.49 PNYY308 pKa = 9.65 KK309 pKa = 10.2 SLAGRR314 pKa = 11.84 LKK316 pKa = 10.61 SHH318 pKa = 7.24 FKK320 pKa = 10.4 RR321 pKa = 11.84 AGVNNIGFHH330 pKa = 5.79 GFRR333 pKa = 11.84 HH334 pKa = 4.87 THH336 pKa = 6.58 ASLLLNSGIPYY347 pKa = 10.0 KK348 pKa = 10.34 EE349 pKa = 3.8 LQHH352 pKa = 6.73 RR353 pKa = 11.84 LGHH356 pKa = 5.68 STLSMTMDD364 pKa = 4.17 TYY366 pKa = 11.8 SHH368 pKa = 7.15 LSKK371 pKa = 11.01 EE372 pKa = 4.3 NAKK375 pKa = 10.31 KK376 pKa = 9.35 ATSFYY381 pKa = 10.1 EE382 pKa = 3.9 QALKK386 pKa = 10.8 SII388 pKa = 4.54
Molecular weight: 44.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.024
IPC2_protein 9.151
IPC_protein 9.004
Toseland 9.823
ProMoST 9.502
Dawson 10.043
Bjellqvist 9.692
Wikipedia 10.189
Rodwell 10.555
Grimsley 10.116
Solomon 10.058
Lehninger 10.014
Nozaki 9.809
DTASelect 9.677
Thurlkill 9.882
EMBOSS 10.233
Sillero 9.94
Patrickios 9.736
IPC_peptide 10.058
IPC2_peptide 8.141
IPC2.peptide.svr19 8.064
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1563
78
388
195.4
22.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.374 ± 0.355
0.576 ± 0.192
5.95 ± 0.573
7.55 ± 1.135
3.519 ± 0.419
5.758 ± 0.505
1.663 ± 0.44
6.334 ± 0.755
9.789 ± 0.763
9.789 ± 0.857
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.239 ± 0.465
5.886 ± 0.664
3.263 ± 0.799
4.351 ± 0.639
4.862 ± 0.495
5.502 ± 0.399
6.59 ± 0.759
5.246 ± 0.399
0.832 ± 0.186
4.926 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here