Tissierella creatinini
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2472 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4T9WIG4|A0A4T9WIG4_9FIRM DUF5067 domain-containing protein OS=Tissierella creatinini OX=43143 GN=E9840_01910 PE=4 SV=1
MM1 pKa = 7.18 SACTAANDD9 pKa = 3.66 SDD11 pKa = 5.43 LEE13 pKa = 4.22 EE14 pKa = 4.14 EE15 pKa = 4.27 LQAKK19 pKa = 9.91 KK20 pKa = 10.58 EE21 pKa = 4.06 IIATLEE27 pKa = 4.03 SEE29 pKa = 4.16 NEE31 pKa = 3.91 KK32 pKa = 10.76 LQEE35 pKa = 4.85 EE36 pKa = 4.4 INKK39 pKa = 9.7 LNNRR43 pKa = 11.84 IEE45 pKa = 4.16 EE46 pKa = 4.53 LEE48 pKa = 4.36 GEE50 pKa = 4.64 TPSQTQATSYY60 pKa = 9.3 LTAALEE66 pKa = 4.1 AVEE69 pKa = 5.35 LLSNDD74 pKa = 4.04 DD75 pKa = 3.95 MNGLASMAHH84 pKa = 5.71 PVKK87 pKa = 10.67 GIRR90 pKa = 11.84 FTPYY94 pKa = 10.53 SYY96 pKa = 10.84 IDD98 pKa = 3.46 TSTDD102 pKa = 3.24 QVFTANQISNLMTDD116 pKa = 3.32 TTVYY120 pKa = 9.26 TWGNYY125 pKa = 9.41 DD126 pKa = 4.09 GSGEE130 pKa = 4.34 PINKK134 pKa = 8.37 TFADD138 pKa = 4.29 YY139 pKa = 11.31 YY140 pKa = 11.39 DD141 pKa = 3.66 EE142 pKa = 4.71 FVYY145 pKa = 10.99 DD146 pKa = 3.62 EE147 pKa = 5.91 DD148 pKa = 4.14 YY149 pKa = 11.59 ANPHH153 pKa = 6.59 IIGNNTLIGKK163 pKa = 9.29 GNSIDD168 pKa = 3.93 NLTDD172 pKa = 2.98 VYY174 pKa = 11.24 TNGEE178 pKa = 3.87 FVEE181 pKa = 4.85 FHH183 pKa = 5.67 FTGFDD188 pKa = 3.23 AQYY191 pKa = 11.32 EE192 pKa = 4.43 GMDD195 pKa = 3.33 WSSLKK200 pKa = 10.83 LVFEE204 pKa = 4.58 EE205 pKa = 6.21 DD206 pKa = 2.97 SGIWYY211 pKa = 9.45 LVAIIHH217 pKa = 5.55 SQWTII222 pKa = 2.94
Molecular weight: 24.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.923
IPC_protein 3.897
Toseland 3.706
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.745
Rodwell 3.719
Grimsley 3.617
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.139
Thurlkill 3.732
EMBOSS 3.757
Sillero 3.999
Patrickios 0.871
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.902
Protein with the highest isoelectric point:
>tr|A0A4T9WA50|A0A4T9WA50_9FIRM Uncharacterized protein OS=Tissierella creatinini OX=43143 GN=E9840_12540 PE=4 SV=1
RR1 pKa = 7.14 NGKK4 pKa = 6.76 TLEE7 pKa = 4.17 SRR9 pKa = 11.84 SGEE12 pKa = 3.8 AWSEE16 pKa = 3.82 GFIRR20 pKa = 11.84 DD21 pKa = 3.85 LEE23 pKa = 5.37 AITKK27 pKa = 7.1 MTFKK31 pKa = 10.5 RR32 pKa = 11.84 RR33 pKa = 11.84 NGGRR37 pKa = 11.84 NKK39 pKa = 10.07 HH40 pKa = 5.44 GRR42 pKa = 11.84 GHH44 pKa = 5.86 VKK46 pKa = 10.46 FIRR49 pKa = 11.84 CSNCGKK55 pKa = 10.17 CCPKK59 pKa = 10.69 DD60 pKa = 3.53 KK61 pKa = 10.6 AIKK64 pKa = 10.12 RR65 pKa = 11.84 FLVRR69 pKa = 11.84 NIVEE73 pKa = 4.03 QAAVRR78 pKa = 11.84 DD79 pKa = 4.08 VQEE82 pKa = 3.95 ACAFEE87 pKa = 5.95 SYY89 pKa = 9.58 TLPKK93 pKa = 10.3 LYY95 pKa = 11.06 VKK97 pKa = 9.54 MQYY100 pKa = 10.13 CVSCAIHH107 pKa = 6.07 SKK109 pKa = 8.2 VVRR112 pKa = 11.84 VRR114 pKa = 11.84 SRR116 pKa = 11.84 TDD118 pKa = 2.68 RR119 pKa = 11.84 RR120 pKa = 11.84 NRR122 pKa = 11.84 EE123 pKa = 3.46 PPQRR127 pKa = 11.84 FRR129 pKa = 11.84 RR130 pKa = 11.84 RR131 pKa = 11.84 DD132 pKa = 3.51 DD133 pKa = 3.48 AQKK136 pKa = 10.34 PGQAPRR142 pKa = 11.84 LAGAAGPPRR151 pKa = 11.84 PP152 pKa = 4.07
Molecular weight: 17.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.648
IPC_protein 10.409
Toseland 10.891
ProMoST 10.774
Dawson 10.935
Bjellqvist 10.657
Wikipedia 11.155
Rodwell 11.155
Grimsley 10.965
Solomon 11.111
Lehninger 11.067
Nozaki 10.891
DTASelect 10.643
Thurlkill 10.862
EMBOSS 11.301
Sillero 10.891
Patrickios 10.877
IPC_peptide 11.111
IPC2_peptide 9.897
IPC2.peptide.svr19 8.761
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2472
0
2472
744290
22
1444
301.1
33.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.904 ± 0.052
0.925 ± 0.018
5.995 ± 0.044
7.55 ± 0.058
4.183 ± 0.037
6.96 ± 0.046
1.534 ± 0.019
9.583 ± 0.057
7.986 ± 0.05
9.511 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.731 ± 0.022
5.438 ± 0.034
3.219 ± 0.023
2.457 ± 0.022
4.003 ± 0.028
6.122 ± 0.037
4.842 ± 0.031
6.384 ± 0.048
0.672 ± 0.015
4.002 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here