Pythium brassicum

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Pythiales; Pythiaceae; Pythium

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12998 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5D6XSK8|A0A5D6XSK8_9STRA Uncharacterized protein OS=Pythium brassicum OX=1485010 GN=PybrP1_010047 PE=4 SV=1
MM1 pKa = 7.4TKK3 pKa = 10.1SLHH6 pKa = 5.76LSXVAVTAAAVALLVDD22 pKa = 3.73SGIDD26 pKa = 3.05RR27 pKa = 11.84FGSVGVFAQTTGTAAATVAICRR49 pKa = 11.84APASSLTDD57 pKa = 3.3YY58 pKa = 11.35GVGIVSDD65 pKa = 4.48PACATPSSAASSSCIDD81 pKa = 3.63DD82 pKa = 3.14QCRR85 pKa = 11.84FCQVFRR91 pKa = 11.84TDD93 pKa = 3.2LSKK96 pKa = 11.21GYY98 pKa = 9.79LPCTTPPVLVSGGAGVPPSEE118 pKa = 4.57TPPSPSVPPTTLVPTLASALDD139 pKa = 3.87PTPTAPAAVTFEE151 pKa = 4.29PTVVAPAPASVSMTPVPSAVGIALGAVPATAPPISPLLIDD191 pKa = 4.63CSAEE195 pKa = 3.87LSLEE199 pKa = 3.9AMAAGVYY206 pKa = 10.15AVLDD210 pKa = 4.3PSCQADD216 pKa = 3.71DD217 pKa = 4.54DD218 pKa = 4.49AAQASCQDD226 pKa = 3.64EE227 pKa = 4.56FCRR230 pKa = 11.84LCRR233 pKa = 11.84VGEE236 pKa = 3.95SDD238 pKa = 3.97YY239 pKa = 11.81AANTDD244 pKa = 4.63DD245 pKa = 5.38AGADD249 pKa = 3.82PGTDD253 pKa = 3.58DD254 pKa = 4.57IGANSSADD262 pKa = 3.64NPGTDD267 pKa = 4.25PGTDD271 pKa = 4.43DD272 pKa = 4.33IDD274 pKa = 5.47ADD276 pKa = 4.06SSPDD280 pKa = 4.2DD281 pKa = 3.89VDD283 pKa = 4.94ADD285 pKa = 4.22SDD287 pKa = 4.06TDD289 pKa = 5.84DD290 pKa = 4.13IDD292 pKa = 5.89ADD294 pKa = 3.73SSTNNDD300 pKa = 3.41GADD303 pKa = 3.36TNTNIVGANTRR314 pKa = 11.84TNNASADD321 pKa = 3.55PRR323 pKa = 11.84ANNAGADD330 pKa = 3.75SSANDD335 pKa = 3.54HH336 pKa = 6.96DD337 pKa = 5.33ANPNADD343 pKa = 3.89DD344 pKa = 4.48FGAGSSPDD352 pKa = 3.49DD353 pKa = 4.33HH354 pKa = 6.6GTDD357 pKa = 4.06PGTDD361 pKa = 4.32DD362 pKa = 4.31IDD364 pKa = 5.38ADD366 pKa = 3.76SSTDD370 pKa = 3.9FVSDD374 pKa = 3.63PTADD378 pKa = 3.56NTPEE382 pKa = 4.05PTPVPTTLVPTPLPTTLAPTPVPTTLAPTPAPTTLAPTPVPTTAAPAPTTGAPVNFDD439 pKa = 5.67LIDD442 pKa = 4.11CNEE445 pKa = 4.6NISIDD450 pKa = 3.54AMSAGIYY457 pKa = 10.12SVLDD461 pKa = 3.57KK462 pKa = 11.25TCMTEE467 pKa = 4.44FDD469 pKa = 4.7PLSEE473 pKa = 4.29TTTCMTKK480 pKa = 9.99YY481 pKa = 10.48CRR483 pKa = 11.84LCKK486 pKa = 10.44VADD489 pKa = 3.88TEE491 pKa = 4.43DD492 pKa = 3.79TVGLKK497 pKa = 10.1SCSQFFSFRR506 pKa = 11.84RR507 pKa = 11.84HH508 pKa = 4.51LVRR511 pKa = 11.84RR512 pKa = 11.84AAA514 pKa = 3.78

Molecular weight: 51.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5D6Y9F7|A0A5D6Y9F7_9STRA Uncharacterized protein OS=Pythium brassicum OX=1485010 GN=PybrP1_007566 PE=4 SV=1
MM1 pKa = 7.44RR2 pKa = 11.84QRR4 pKa = 11.84LRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84PCRR11 pKa = 11.84PRR13 pKa = 11.84VRR15 pKa = 11.84QRR17 pKa = 11.84LCRR20 pKa = 11.84PLACRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84CRR29 pKa = 11.84HH30 pKa = 4.86PVCRR34 pKa = 11.84PVRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84LPRR42 pKa = 11.84PRR44 pKa = 11.84VRR46 pKa = 11.84RR47 pKa = 11.84PPARR51 pKa = 11.84RR52 pKa = 11.84SASSTATTRR61 pKa = 11.84TTAPGTGRR69 pKa = 11.84RR70 pKa = 11.84TRR72 pKa = 11.84ASATT76 pKa = 3.39

Molecular weight: 9.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12998

0

12998

6730507

8

7627

517.8

56.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.36 ± 0.034

1.599 ± 0.01

5.759 ± 0.013

5.94 ± 0.022

3.656 ± 0.015

6.459 ± 0.019

2.569 ± 0.011

3.121 ± 0.013

4.089 ± 0.017

9.782 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.886 ± 0.009

2.643 ± 0.011

4.999 ± 0.019

3.965 ± 0.014

7.237 ± 0.022

8.021 ± 0.027

5.23 ± 0.016

7.385 ± 0.02

1.12 ± 0.006

2.165 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski