Amino acid dipepetide frequency for Pythium brassicum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.603AlaAla: 19.603 ± 0.11	1.654AlaCys: 1.654 ± 0.022	6.264AlaAsp: 6.264 ± 0.04	6.137AlaGlu: 6.137 ± 0.042	4.246AlaPhe: 4.246 ± 0.025	7.239AlaGly: 7.239 ± 0.037	2.95AlaHis: 2.95 ± 0.025	3.654AlaIle: 3.654 ± 0.023	4.932AlaLys: 4.932 ± 0.035	12.635AlaLeu: 12.635 ± 0.069	2.346AlaMet: 2.346 ± 0.02	2.978AlaAsn: 2.978 ± 0.026	6.548AlaPro: 6.548 ± 0.051	4.583AlaGln: 4.583 ± 0.033	8.316AlaArg: 8.316 ± 0.052	10.482AlaSer: 10.482 ± 0.052	7.436AlaThr: 7.436 ± 0.041	8.383AlaVal: 8.383 ± 0.044	1.12AlaTrp: 1.12 ± 0.015	2.093AlaTyr: 2.093 ± 0.02	0.003AlaXaa: 0.003 ± 0.001
Cys	1.835CysAla: 1.835 ± 0.021	0.396CysCys: 0.396 ± 0.01	0.895CysAsp: 0.895 ± 0.016	0.934CysGlu: 0.934 ± 0.014	0.666CysPhe: 0.666 ± 0.011	1.239CysGly: 1.239 ± 0.015	0.35CysHis: 0.35 ± 0.007	0.545CysIle: 0.545 ± 0.009	0.58CysLys: 0.58 ± 0.012	1.346CysLeu: 1.346 ± 0.016	0.305CysMet: 0.305 ± 0.007	0.409CysAsn: 0.409 ± 0.008	0.719CysPro: 0.719 ± 0.023	0.467CysGln: 0.467 ± 0.01	1.098CysArg: 1.098 ± 0.014	1.278CysSer: 1.278 ± 0.018	0.86CysThr: 0.86 ± 0.014	1.476CysVal: 1.476 ± 0.016	0.216CysTrp: 0.216 ± 0.006	0.374CysTyr: 0.374 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	8.657AspAla: 8.657 ± 0.051	0.739AspCys: 0.739 ± 0.013	5.817AspAsp: 5.817 ± 0.059	4.718AspGlu: 4.718 ± 0.036	1.999AspPhe: 1.999 ± 0.019	4.446AspGly: 4.446 ± 0.033	1.093AspHis: 1.093 ± 0.012	1.834AspIle: 1.834 ± 0.02	1.934AspLys: 1.934 ± 0.019	4.292AspLeu: 4.292 ± 0.027	1.012AspMet: 1.012 ± 0.014	1.412AspAsn: 1.412 ± 0.017	2.516AspPro: 2.516 ± 0.022	1.389AspGln: 1.389 ± 0.016	3.062AspArg: 3.062 ± 0.022	3.941AspSer: 3.941 ± 0.029	2.54AspThr: 2.54 ± 0.02	4.511AspVal: 4.511 ± 0.026	0.613AspTrp: 0.613 ± 0.011	1.066AspTyr: 1.066 ± 0.013	0.002AspXaa: 0.002 ± 0.0
Glu	6.065GluAla: 6.065 ± 0.045	0.93GluCys: 0.93 ± 0.015	3.386GluAsp: 3.386 ± 0.028	4.15GluGlu: 4.15 ± 0.036	2.087GluPhe: 2.087 ± 0.017	2.685GluGly: 2.685 ± 0.025	1.65GluHis: 1.65 ± 0.02	2.261GluIle: 2.261 ± 0.023	2.735GluLys: 2.735 ± 0.028	7.018GluLeu: 7.018 ± 0.046	1.303GluMet: 1.303 ± 0.016	1.65GluAsn: 1.65 ± 0.017	2.539GluPro: 2.539 ± 0.021	2.902GluGln: 2.902 ± 0.029	5.275GluArg: 5.275 ± 0.04	4.248GluSer: 4.248 ± 0.029	2.843GluThr: 2.843 ± 0.023	3.545GluVal: 3.545 ± 0.029	0.747GluTrp: 0.747 ± 0.012	1.379GluTyr: 1.379 ± 0.016	0.001GluXaa: 0.001 ± 0.0
Phe	4.216PheAla: 4.216 ± 0.032	0.701PheCys: 0.701 ± 0.011	2.239PheAsp: 2.239 ± 0.02	2.244PheGlu: 2.244 ± 0.018	1.589PhePhe: 1.589 ± 0.019	2.672PheGly: 2.672 ± 0.028	0.936PheHis: 0.936 ± 0.012	1.094PheIle: 1.094 ± 0.014	1.22PheLys: 1.22 ± 0.015	3.603PheLeu: 3.603 ± 0.032	0.686PheMet: 0.686 ± 0.011	0.975PheAsn: 0.975 ± 0.012	1.615PhePro: 1.615 ± 0.017	1.419PheGln: 1.419 ± 0.016	2.276PheArg: 2.276 ± 0.023	2.63PheSer: 2.63 ± 0.02	1.78PheThr: 1.78 ± 0.017	3.237PheVal: 3.237 ± 0.025	0.415PheTrp: 0.415 ± 0.009	1.009PheTyr: 1.009 ± 0.013	0.0PheXaa: 0.0 ± 0.0
Gly	8.229GlyAla: 8.229 ± 0.048	1.103GlyCys: 1.103 ± 0.017	4.536GlyAsp: 4.536 ± 0.03	3.351GlyGlu: 3.351 ± 0.024	2.455GlyPhe: 2.455 ± 0.024	7.277GlyGly: 7.277 ± 0.065	1.563GlyHis: 1.563 ± 0.019	2.058GlyIle: 2.058 ± 0.019	2.577GlyLys: 2.577 ± 0.023	4.779GlyLeu: 4.779 ± 0.034	1.173GlyMet: 1.173 ± 0.017	1.777GlyAsn: 1.777 ± 0.018	2.109GlyPro: 2.109 ± 0.023	1.627GlyGln: 1.627 ± 0.018	4.053GlyArg: 4.053 ± 0.034	5.728GlySer: 5.728 ± 0.033	2.906GlyThr: 2.906 ± 0.022	5.037GlyVal: 5.037 ± 0.033	0.705GlyTrp: 0.705 ± 0.012	1.54GlyTyr: 1.54 ± 0.019	0.002GlyXaa: 0.002 ± 0.001
His	3.164HisAla: 3.164 ± 0.028	0.447HisCys: 0.447 ± 0.008	1.405HisAsp: 1.405 ± 0.017	1.749HisGlu: 1.749 ± 0.02	1.02HisPhe: 1.02 ± 0.013	1.8HisGly: 1.8 ± 0.021	0.983HisHis: 0.983 ± 0.019	0.767HisIle: 0.767 ± 0.011	0.895HisLys: 0.895 ± 0.014	2.354HisLeu: 2.354 ± 0.022	0.445HisMet: 0.445 ± 0.008	0.604HisAsn: 0.604 ± 0.01	1.309HisPro: 1.309 ± 0.018	1.041HisGln: 1.041 ± 0.014	1.829HisArg: 1.829 ± 0.017	1.751HisSer: 1.751 ± 0.017	1.123HisThr: 1.123 ± 0.012	2.11HisVal: 2.11 ± 0.021	0.322HisTrp: 0.322 ± 0.007	0.577HisTyr: 0.577 ± 0.009	0.0HisXaa: 0.0 ± 0.0
Ile	3.658IleAla: 3.658 ± 0.026	0.601IleCys: 0.601 ± 0.01	1.986IleAsp: 1.986 ± 0.018	2.061IleGlu: 2.061 ± 0.019	1.156IlePhe: 1.156 ± 0.016	2.082IleGly: 2.082 ± 0.02	0.719IleHis: 0.719 ± 0.012	1.015IleIle: 1.015 ± 0.014	1.298IleLys: 1.298 ± 0.015	2.735IleLeu: 2.735 ± 0.027	0.587IleMet: 0.587 ± 0.01	0.933IleAsn: 0.933 ± 0.013	1.564IlePro: 1.564 ± 0.018	1.23IleGln: 1.23 ± 0.015	2.066IleArg: 2.066 ± 0.02	2.167IleSer: 2.167 ± 0.02	1.617IleThr: 1.617 ± 0.017	2.612IleVal: 2.612 ± 0.023	0.336IleTrp: 0.336 ± 0.007	0.791IleTyr: 0.791 ± 0.012	0.0IleXaa: 0.0 ± 0.0
Lys	4.164LysAla: 4.164 ± 0.031	0.656LysCys: 0.656 ± 0.012	1.915LysAsp: 1.915 ± 0.022	2.315LysGlu: 2.315 ± 0.026	1.362LysPhe: 1.362 ± 0.016	1.87LysGly: 1.87 ± 0.021	1.137LysHis: 1.137 ± 0.015	1.484LysIle: 1.484 ± 0.017	3.028LysLys: 3.028 ± 0.03	4.256LysLeu: 4.256 ± 0.033	0.867LysMet: 0.867 ± 0.013	1.262LysAsn: 1.262 ± 0.016	1.937LysPro: 1.937 ± 0.02	1.95LysGln: 1.95 ± 0.021	3.621LysArg: 3.621 ± 0.029	2.808LysSer: 2.808 ± 0.026	2.186LysThr: 2.186 ± 0.02	2.439LysVal: 2.439 ± 0.02	0.559LysTrp: 0.559 ± 0.01	1.071LysTyr: 1.071 ± 0.013	0.001LysXaa: 0.001 ± 0.0
Leu	11.474LeuAla: 11.474 ± 0.059	1.674LeuCys: 1.674 ± 0.018	5.104LeuAsp: 5.104 ± 0.036	6.479LeuGlu: 6.479 ± 0.043	3.584LeuPhe: 3.584 ± 0.034	5.389LeuGly: 5.389 ± 0.03	2.847LeuHis: 2.847 ± 0.024	2.429LeuIle: 2.429 ± 0.026	3.896LeuLys: 3.896 ± 0.029	11.159LeuLeu: 11.159 ± 0.067	1.665LeuMet: 1.665 ± 0.017	2.449LeuAsn: 2.449 ± 0.021	4.961LeuPro: 4.961 ± 0.037	4.753LeuGln: 4.753 ± 0.033	7.68LeuArg: 7.68 ± 0.046	7.437LeuSer: 7.437 ± 0.044	4.387LeuThr: 4.387 ± 0.026	7.31LeuVal: 7.31 ± 0.044	1.047LeuTrp: 1.047 ± 0.013	2.099LeuTyr: 2.099 ± 0.023	0.002LeuXaa: 0.002 ± 0.001
Met	2.056MetAla: 2.056 ± 0.016	0.294MetCys: 0.294 ± 0.007	1.108MetAsp: 1.108 ± 0.015	1.136MetGlu: 1.136 ± 0.013	0.641MetPhe: 0.641 ± 0.01	1.06MetGly: 1.06 ± 0.016	0.495MetHis: 0.495 ± 0.009	0.637MetIle: 0.637 ± 0.011	0.945MetLys: 0.945 ± 0.013	2.003MetLeu: 2.003 ± 0.021	0.47MetMet: 0.47 ± 0.009	0.642MetAsn: 0.642 ± 0.01	0.821MetPro: 0.821 ± 0.012	0.823MetGln: 0.823 ± 0.013	1.396MetArg: 1.396 ± 0.014	1.371MetSer: 1.371 ± 0.015	1.061MetThr: 1.061 ± 0.015	1.201MetVal: 1.201 ± 0.012	0.227MetTrp: 0.227 ± 0.006	0.476MetTyr: 0.476 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	3.364AsnAla: 3.364 ± 0.022	0.462AsnCys: 0.462 ± 0.009	1.639AsnAsp: 1.639 ± 0.017	1.546AsnGlu: 1.546 ± 0.018	0.963AsnPhe: 0.963 ± 0.014	2.149AsnGly: 2.149 ± 0.023	0.607AsnHis: 0.607 ± 0.011	0.942AsnIle: 0.942 ± 0.012	1.046AsnLys: 1.046 ± 0.016	2.222AsnLeu: 2.222 ± 0.024	0.531AsnMet: 0.531 ± 0.009	0.906AsnAsn: 0.906 ± 0.015	1.379AsnPro: 1.379 ± 0.016	0.916AsnGln: 0.916 ± 0.011	1.608AsnArg: 1.608 ± 0.017	1.867AsnSer: 1.867 ± 0.019	1.284AsnThr: 1.284 ± 0.016	2.016AsnVal: 2.016 ± 0.018	0.333AsnTrp: 0.333 ± 0.008	0.651AsnTyr: 0.651 ± 0.011	0.0AsnXaa: 0.0 ± 0.0
Pro	6.147ProAla: 6.147 ± 0.046	0.555ProCys: 0.555 ± 0.014	2.292ProAsp: 2.292 ± 0.022	2.654ProGlu: 2.654 ± 0.024	1.651ProPhe: 1.651 ± 0.017	2.724ProGly: 2.724 ± 0.026	1.249ProHis: 1.249 ± 0.013	1.377ProIle: 1.377 ± 0.017	1.787ProLys: 1.787 ± 0.02	4.72ProLeu: 4.72 ± 0.031	0.853ProMet: 0.853 ± 0.013	1.204ProAsn: 1.204 ± 0.012	4.574ProPro: 4.574 ± 0.057	1.848ProGln: 1.848 ± 0.022	3.389ProArg: 3.389 ± 0.026	4.792ProSer: 4.792 ± 0.038	3.393ProThr: 3.393 ± 0.036	3.477ProVal: 3.477 ± 0.029	0.458ProTrp: 0.458 ± 0.008	0.841ProTyr: 0.841 ± 0.013	0.003ProXaa: 0.003 ± 0.001
Gln	3.662GlnAla: 3.662 ± 0.027	0.622GlnCys: 0.622 ± 0.011	1.654GlnAsp: 1.654 ± 0.02	2.198GlnGlu: 2.198 ± 0.024	1.398GlnPhe: 1.398 ± 0.015	1.579GlnGly: 1.579 ± 0.017	1.401GlnHis: 1.401 ± 0.017	1.272GlnIle: 1.272 ± 0.018	1.455GlnLys: 1.455 ± 0.016	4.953GlnLeu: 4.953 ± 0.034	0.771GlnMet: 0.771 ± 0.011	0.879GlnAsn: 0.879 ± 0.013	2.01GlnPro: 2.01 ± 0.021	3.543GlnGln: 3.543 ± 0.043	3.786GlnArg: 3.786 ± 0.031	2.761GlnSer: 2.761 ± 0.024	1.759GlnThr: 1.759 ± 0.018	2.452GlnVal: 2.452 ± 0.02	0.553GlnTrp: 0.553 ± 0.011	0.942GlnTyr: 0.942 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	8.504ArgAla: 8.504 ± 0.05	1.203ArgCys: 1.203 ± 0.017	3.877ArgAsp: 3.877 ± 0.026	4.806ArgGlu: 4.806 ± 0.037	2.652ArgPhe: 2.652 ± 0.022	4.398ArgGly: 4.398 ± 0.034	2.047ArgHis: 2.047 ± 0.023	2.234ArgIle: 2.234 ± 0.019	3.23ArgLys: 3.23 ± 0.025	6.755ArgLeu: 6.755 ± 0.036	1.347ArgMet: 1.347 ± 0.017	1.793ArgAsn: 1.793 ± 0.017	2.813ArgPro: 2.813 ± 0.025	3.031ArgGln: 3.031 ± 0.024	6.879ArgArg: 6.879 ± 0.059	5.459ArgSer: 5.459 ± 0.037	3.246ArgThr: 3.246 ± 0.023	5.78ArgVal: 5.78 ± 0.036	0.886ArgTrp: 0.886 ± 0.01	1.431ArgTyr: 1.431 ± 0.015	0.003ArgXaa: 0.003 ± 0.001
Ser	10.115SerAla: 10.115 ± 0.047	1.167SerCys: 1.167 ± 0.017	4.705SerAsp: 4.705 ± 0.034	4.141SerGlu: 4.141 ± 0.029	2.824SerPhe: 2.824 ± 0.024	6.031SerGly: 6.031 ± 0.036	1.644SerHis: 1.644 ± 0.017	2.457SerIle: 2.457 ± 0.022	2.924SerLys: 2.924 ± 0.024	6.703SerLeu: 6.703 ± 0.04	1.426SerMet: 1.426 ± 0.015	2.073SerAsn: 2.073 ± 0.02	4.407SerPro: 4.407 ± 0.035	2.278SerGln: 2.278 ± 0.021	4.943SerArg: 4.943 ± 0.034	9.76SerSer: 9.76 ± 0.075	4.569SerThr: 4.569 ± 0.032	5.669SerVal: 5.669 ± 0.035	0.828SerTrp: 0.828 ± 0.013	1.545SerTyr: 1.545 ± 0.015	0.003SerXaa: 0.003 ± 0.001
Thr	6.236ThrAla: 6.236 ± 0.036	0.815ThrCys: 0.815 ± 0.012	2.439ThrAsp: 2.439 ± 0.019	2.498ThrGlu: 2.498 ± 0.023	1.805ThrPhe: 1.805 ± 0.019	3.355ThrGly: 3.355 ± 0.025	1.274ThrHis: 1.274 ± 0.013	1.836ThrIle: 1.836 ± 0.019	2.218ThrLys: 2.218 ± 0.021	5.056ThrLeu: 5.056 ± 0.032	0.995ThrMet: 0.995 ± 0.013	1.446ThrAsn: 1.446 ± 0.016	3.517ThrPro: 3.517 ± 0.038	1.83ThrGln: 1.83 ± 0.017	3.54ThrArg: 3.54 ± 0.026	4.422ThrSer: 4.422 ± 0.031	3.924ThrThr: 3.924 ± 0.038	3.474ThrVal: 3.474 ± 0.029	0.557ThrTrp: 0.557 ± 0.01	1.06ThrTyr: 1.06 ± 0.013	0.002ThrXaa: 0.002 ± 0.0
Val	9.301ValAla: 9.301 ± 0.045	1.321ValCys: 1.321 ± 0.018	4.392ValAsp: 4.392 ± 0.026	4.334ValGlu: 4.334 ± 0.03	2.954ValPhe: 2.954 ± 0.027	4.512ValGly: 4.512 ± 0.031	1.882ValHis: 1.882 ± 0.019	2.159ValIle: 2.159 ± 0.02	2.754ValLys: 2.754 ± 0.026	7.855ValLeu: 7.855 ± 0.049	1.313ValMet: 1.313 ± 0.015	1.942ValAsn: 1.942 ± 0.018	3.37ValPro: 3.37 ± 0.025	2.8ValGln: 2.8 ± 0.021	5.12ValArg: 5.12 ± 0.035	4.966ValSer: 4.966 ± 0.033	3.615ValThr: 3.615 ± 0.034	6.703ValVal: 6.703 ± 0.042	0.83ValTrp: 0.83 ± 0.011	1.719ValTyr: 1.719 ± 0.018	0.002ValXaa: 0.002 ± 0.001
Trp	0.824TrpAla: 0.824 ± 0.011	0.201TrpCys: 0.201 ± 0.006	0.623TrpAsp: 0.623 ± 0.01	0.602TrpGlu: 0.602 ± 0.01	0.443TrpPhe: 0.443 ± 0.008	0.631TrpGly: 0.631 ± 0.011	0.29TrpHis: 0.29 ± 0.007	0.422TrpIle: 0.422 ± 0.008	0.611TrpLys: 0.611 ± 0.011	1.298TrpLeu: 1.298 ± 0.02	0.291TrpMet: 0.291 ± 0.007	0.394TrpAsn: 0.394 ± 0.009	0.373TrpPro: 0.373 ± 0.009	0.442TrpGln: 0.442 ± 0.008	1.039TrpArg: 1.039 ± 0.014	0.846TrpSer: 0.846 ± 0.013	0.671TrpThr: 0.671 ± 0.012	0.733TrpVal: 0.733 ± 0.012	0.172TrpTrp: 0.172 ± 0.006	0.297TrpTyr: 0.297 ± 0.007	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.326TyrAla: 2.326 ± 0.02	0.448TyrCys: 0.448 ± 0.012	1.313TyrAsp: 1.313 ± 0.014	1.345TyrGlu: 1.345 ± 0.015	1.06TyrPhe: 1.06 ± 0.014	1.447TyrGly: 1.447 ± 0.016	0.576TyrHis: 0.576 ± 0.008	0.735TyrIle: 0.735 ± 0.012	0.824TyrLys: 0.824 ± 0.012	2.082TyrLeu: 2.082 ± 0.021	0.475TyrMet: 0.475 ± 0.009	0.704TyrAsn: 0.704 ± 0.01	0.905TyrPro: 0.905 ± 0.015	0.778TyrGln: 0.778 ± 0.012	1.394TyrArg: 1.394 ± 0.017	1.493TyrSer: 1.493 ± 0.015	1.093TyrThr: 1.093 ± 0.015	1.68TyrVal: 1.68 ± 0.018	0.281TyrTrp: 0.281 ± 0.007	0.693TyrTyr: 0.693 ± 0.011	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.002XaaLeu: 0.002 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.001	0.001XaaGln: 0.001 ± 0.0	0.003XaaArg: 0.003 ± 0.001	0.001XaaSer: 0.001 ± 0.0	0.002XaaThr: 0.002 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.113XaaXaa: 0.113 ± 0.013

Statistics based on 12998 proteins (6730507 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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